BacMap

Definition	Vibrio fischeri ES114 chromosome I, complete genome.
Accession	NC_006840
Length	2,897,536

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The map label for this gene is cysJ

Identifier: 59710917

GI number: 59710917

Start: 318660

End: 320474

Strand: Direct

Name: cysJ

Synonym: VF_0310

Alternate gene names: 59710917

Gene position: 318660-320474 (Clockwise)

Preceding gene: 59710916

Following gene: 59710918

Centisome position: 11.0

GC content: 41.93

Gene sequence:

>1815_bases
ATGTTATTAAAGGAACTCTCCGCACTCGCTAGTCCACTAAATGACCAACAAATAGGTCAGTTACAGCAAGCCGCTTCTGA
ATTGTCGCCACAGCAATTGGCGTGGGTCAGTGGCTACTTCTGGGGGATAAGCCAAACATCAGGTGCAACACAACCAATCA
ATCAAGCTGCCGCAGCGGTATCAAGCCAACCTGCTGGTAAGTTATCTATTATTTTTGCCTCTCAAACCGGTAATGCCAAA
GGCGTTGCTGAAGCATTAAAAGAAGAAGCGGCAGCGGCAGGTATTGCTGTTGAGCTTTTTGATGCCAGTGATTACAAAGG
TAAACATTTAGCAAAAGAAACTCATGTCATTATTGTCGCTTCAACCAATGGTGAAGGTGAGGCACCTGATAATGCCATTG
AGTTACATGAATTTTTACAATCGAAAAAGGCACCTAAGCTCGATAATTTACATTACGGTGTTATTGGTCTAGGGGATTCA
AGTTATGAGTTTTTCTGCCAAACAGGTAAAGATTTTGATGCCTTCTTATCAAAACAAGGGGCAACACCGTTTATTGAACG
TGTTGATCTTGATGTAGATTATGAAGCTCCGGCTGCAGAGTGGCGAAAGCAAGCTCTTGATAAAGTAAAAGAGGCATTAG
CTTCTGAAGCACAAAGTGCGCAAGTGGTTCAGCTTCCTGTTGGACAAGCCGCTCATACCTCTCAATACTCAAAACAAAAT
CCGTATACCGCTACGCTATTAACTAGCCAAAAAATCACAGGGCGAGATTCAGGTAAAGATGTTCGTCATATCGAAATTGA
TTTAGATGGTTCAGGATTAACTTATCAACCGGGTGATGCTCTTGGTGTGTGGTTTGAAAATAGCCCAGAGCTCGCTTCAG
CAATTTTGAAGCAAGTCGGTTTAACGGGTGATGAAGCGGTTGAGGTAGATGGTGATTCAATATCGCTTCAAAAAGCCTTG
GTTGAAAAATATGAAATCACCTCAGCAAATCCGCAATTAGTGACTCAATATGCTGAGTTGTCAGGCAGCAAAAAGTTAGA
AAAGCTAGCAGCAGATAAAGATAAGCTTCGCCAGTATTCAGGGAATACTCAAGTTATTGATGTATTGTCTGAGAAAAAAG
CAAAACTCACCGCAGAGCAACTGGTTGGTTTGCTTCGTCGCTTAACTCCTCGTCTATATTCTATTGCATCAAGTCAGTCT
GAAGTCGATGAAGAAGTGCATTTGACGGTAGGGGTTGTGGAGTATCTGCAAGGAGATGAAACACGTTTTGGTGGTGCATC
TAGCTTTTTATCTCATCGCTTAGAAGAAGGCGATGATGTGAAAGTATTTGTTGAACACAATAACAATTTCAAATTACCTC
AAGATGATAATGCCCCAGTGATAATGATTGGACCAGGTACTGGTATTGCCCCATTCCGCTCATTTGTACAAGAGCGTGAT
AACCGTGATGCAGAAGGTAAAAACTGGTTATTCTTTGGTGATAGAATCTTTACTCAAGATTTTTTATATCAGGTGGAGTG
GCAGAAATACCTTAAATCGGGAATTGTGAATCAGCTTGATGTTGCTTTTAGTCGTGACCAACAAGAAAAAGTATATGTAC
AACACCGAATCTTAGAGCATGCCGCACAAGTATGGCAGTGGCTGCAAGATGGGGCGTATATCTATGTATGTGGTGACGCA
ACTCGTATGGCGAAAGACGTACACGAAGCATTGATTTGCGTTGTAGAGCAACATGGACAGAAAACGAGAGAAGAAGCAGA
GCAATTTGTAAATGAGCTGCGTAAGGCAAAACGTTATCAAAGGGATGTGTACTAA

Upstream 100 bases:

>100_bases
TTTCTTTCTTCCAAAAAGTTATAAAACATACTTATCTATTCTTTCTTGTTATAGAAAATTCTCGCTAATCTAACATGACG
CAATGAATAGGGATGTCGTC

Downstream 100 bases:

>100_bases
TGAGCGAATTAATTGACGTTAAAACGATTTTATCAGGGACAGAGCTTGGTCCACTTGCTGATAACGAGCGATTAAAGCGT
GAAAGTAAGTATTTACGTGG

Product: sulfite reductase, alpha subunit, flavoprotein

Products: NA

Alternate protein names: SiR-FP

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MLLKELSALASPLNDQQIGQLQQAASELSPQQLAWVSGYFWGISQTSGATQPINQAAAAVSSQPAGKLSIIFASQTGNAK
GVAEALKEEAAAAGIAVELFDASDYKGKHLAKETHVIIVASTNGEGEAPDNAIELHEFLQSKKAPKLDNLHYGVIGLGDS
SYEFFCQTGKDFDAFLSKQGATPFIERVDLDVDYEAPAAEWRKQALDKVKEALASEAQSAQVVQLPVGQAAHTSQYSKQN
PYTATLLTSQKITGRDSGKDVRHIEIDLDGSGLTYQPGDALGVWFENSPELASAILKQVGLTGDEAVEVDGDSISLQKAL
VEKYEITSANPQLVTQYAELSGSKKLEKLAADKDKLRQYSGNTQVIDVLSEKKAKLTAEQLVGLLRRLTPRLYSIASSQS
EVDEEVHLTVGVVEYLQGDETRFGGASSFLSHRLEEGDDVKVFVEHNNNFKLPQDDNAPVIMIGPGTGIAPFRSFVQERD
NRDAEGKNWLFFGDRIFTQDFLYQVEWQKYLKSGIVNQLDVAFSRDQQEKVYVQHRILEHAAQVWQWLQDGAYIYVCGDA
TRMAKDVHEALICVVEQHGQKTREEAEQFVNELRKAKRYQRDVY

Sequences:

>Translated_604_residues
MLLKELSALASPLNDQQIGQLQQAASELSPQQLAWVSGYFWGISQTSGATQPINQAAAAVSSQPAGKLSIIFASQTGNAK
GVAEALKEEAAAAGIAVELFDASDYKGKHLAKETHVIIVASTNGEGEAPDNAIELHEFLQSKKAPKLDNLHYGVIGLGDS
SYEFFCQTGKDFDAFLSKQGATPFIERVDLDVDYEAPAAEWRKQALDKVKEALASEAQSAQVVQLPVGQAAHTSQYSKQN
PYTATLLTSQKITGRDSGKDVRHIEIDLDGSGLTYQPGDALGVWFENSPELASAILKQVGLTGDEAVEVDGDSISLQKAL
VEKYEITSANPQLVTQYAELSGSKKLEKLAADKDKLRQYSGNTQVIDVLSEKKAKLTAEQLVGLLRRLTPRLYSIASSQS
EVDEEVHLTVGVVEYLQGDETRFGGASSFLSHRLEEGDDVKVFVEHNNNFKLPQDDNAPVIMIGPGTGIAPFRSFVQERD
NRDAEGKNWLFFGDRIFTQDFLYQVEWQKYLKSGIVNQLDVAFSRDQQEKVYVQHRILEHAAQVWQWLQDGAYIYVCGDA
TRMAKDVHEALICVVEQHGQKTREEAEQFVNELRKAKRYQRDVY
>Mature_604_residues
MLLKELSALASPLNDQQIGQLQQAASELSPQQLAWVSGYFWGISQTSGATQPINQAAAAVSSQPAGKLSIIFASQTGNAK
GVAEALKEEAAAAGIAVELFDASDYKGKHLAKETHVIIVASTNGEGEAPDNAIELHEFLQSKKAPKLDNLHYGVIGLGDS
SYEFFCQTGKDFDAFLSKQGATPFIERVDLDVDYEAPAAEWRKQALDKVKEALASEAQSAQVVQLPVGQAAHTSQYSKQN
PYTATLLTSQKITGRDSGKDVRHIEIDLDGSGLTYQPGDALGVWFENSPELASAILKQVGLTGDEAVEVDGDSISLQKAL
VEKYEITSANPQLVTQYAELSGSKKLEKLAADKDKLRQYSGNTQVIDVLSEKKAKLTAEQLVGLLRRLTPRLYSIASSQS
EVDEEVHLTVGVVEYLQGDETRFGGASSFLSHRLEEGDDVKVFVEHNNNFKLPQDDNAPVIMIGPGTGIAPFRSFVQERD
NRDAEGKNWLFFGDRIFTQDFLYQVEWQKYLKSGIVNQLDVAFSRDQQEKVYVQHRILEHAAQVWQWLQDGAYIYVCGDA
TRMAKDVHEALICVVEQHGQKTREEAEQFVNELRKAKRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain

Homologues:

Organism=Homo sapiens, GI7657393, Length=601, Percent_Identity=29.450915141431, Blast_Score=225, Evalue=1e-58,
Organism=Homo sapiens, GI221316709, Length=601, Percent_Identity=29.450915141431, Blast_Score=221, Evalue=2e-57,
Organism=Homo sapiens, GI127139033, Length=621, Percent_Identity=28.6634460547504, Blast_Score=220, Evalue=3e-57,
Organism=Homo sapiens, GI221316705, Length=610, Percent_Identity=29.016393442623, Blast_Score=218, Evalue=2e-56,
Organism=Homo sapiens, GI24041029, Length=600, Percent_Identity=30.5, Blast_Score=211, Evalue=2e-54,
Organism=Homo sapiens, GI221316707, Length=531, Percent_Identity=30.8851224105461, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI10835173, Length=645, Percent_Identity=27.2868217054264, Blast_Score=185, Evalue=1e-46,
Organism=Homo sapiens, GI40254422, Length=658, Percent_Identity=26.7477203647416, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI169790956, Length=416, Percent_Identity=29.5673076923077, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI169790958, Length=415, Percent_Identity=29.1566265060241, Blast_Score=158, Evalue=2e-38,
Organism=Escherichia coli, GI1789123, Length=593, Percent_Identity=53.2883642495784, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI1789154, Length=134, Percent_Identity=33.5820895522388, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790181, Length=148, Percent_Identity=31.7567567567568, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17554134, Length=614, Percent_Identity=30.6188925081433, Blast_Score=248, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI17566446, Length=571, Percent_Identity=27.4956217162872, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17531441, Length=420, Percent_Identity=24.2857142857143, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32563863, Length=125, Percent_Identity=34.4, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321832, Length=656, Percent_Identity=29.8780487804878, Blast_Score=214, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6321143, Length=386, Percent_Identity=31.3471502590674, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6325305, Length=631, Percent_Identity=22.8209191759113, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17137192, Length=599, Percent_Identity=27.7128547579299, Blast_Score=213, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24582192, Length=512, Percent_Identity=27.9296875, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24660907, Length=597, Percent_Identity=28.9782244556114, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24660903, Length=597, Percent_Identity=28.9782244556114, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI78706872, Length=523, Percent_Identity=30.019120458891, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24583543, Length=523, Percent_Identity=30.019120458891, Blast_Score=172, Evalue=6e-43,
Organism=Drosophila melanogaster, GI78706876, Length=523, Percent_Identity=30.019120458891, Blast_Score=172, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24647438, Length=391, Percent_Identity=24.2966751918159, Blast_Score=82, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSJ_VIBF1 (Q5E841)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203693.1
- ProteinModelPortal:   Q5E841
- SMR:   Q5E841
- STRING:   Q5E841
- GeneID:   3277261
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0310
- NMPDR:   fig|312309.3.peg.310
- eggNOG:   COG0369
- HOGENOM:   HBG736048
- OMA:   DAPDDAI
- PhylomeDB:   Q5E841
- ProtClustDB:   CLSK873955
- BioCyc:   VFIS312309:VF0310-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01541
- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938
- Gene3D:   G3DSA:1.20.990.10
- PIRSF:   PIRSF000207
- PRINTS:   PR00369
- PRINTS:   PR00371
- TIGRFAMs:   TIGR01931

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =1.8.1.2

Molecular weight: Translated: 66652; Mature: 66652

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS51384 FAD_FR; PS50902 FLAVODOXIN_LIKE

Important sites: BINDING 494-494

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKELSALASPLNDQQIGQLQQAASELSPQQLAWVSGYFWGISQTSGATQPINQAAAAV
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCCHHHHHHHHH
SSQPAGKLSIIFASQTGNAKGVAEALKEEAAAAGIAVELFDASDYKGKHLAKETHVIIVA
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEEEE
STNGEGEAPDNAIELHEFLQSKKAPKLDNLHYGVIGLGDSSYEFFCQTGKDFDAFLSKQG
ECCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEECCCCCHHHHHHCCC
ATPFIERVDLDVDYEAPAAEWRKQALDKVKEALASEAQSAQVVQLPVGQAAHTSQYSKQN
CCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCC
PYTATLLTSQKITGRDSGKDVRHIEIDLDGSGLTYQPGDALGVWFENSPELASAILKQVG
CEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHC
LTGDEAVEVDGDSISLQKALVEKYEITSANPQLVTQYAELSGSKKLEKLAADKDKLRQYS
CCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHC
GNTQVIDVLSEKKAKLTAEQLVGLLRRLTPRLYSIASSQSEVDEEVHLTVGVVEYLQGDE
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCCC
TRFGGASSFLSHRLEEGDDVKVFVEHNNNFKLPQDDNAPVIMIGPGTGIAPFRSFVQERD
CCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHC
NRDAEGKNWLFFGDRIFTQDFLYQVEWQKYLKSGIVNQLDVAFSRDQQEKVYVQHRILEH
CCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHH
AAQVWQWLQDGAYIYVCGDATRMAKDVHEALICVVEQHGQKTREEAEQFVNELRKAKRYQ
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
RDVY
HCCC
>Mature Secondary Structure
MLLKELSALASPLNDQQIGQLQQAASELSPQQLAWVSGYFWGISQTSGATQPINQAAAAV
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCCHHHHHHHHH
SSQPAGKLSIIFASQTGNAKGVAEALKEEAAAAGIAVELFDASDYKGKHLAKETHVIIVA
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEEEE
STNGEGEAPDNAIELHEFLQSKKAPKLDNLHYGVIGLGDSSYEFFCQTGKDFDAFLSKQG
ECCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEECCCCCHHHHHHCCC
ATPFIERVDLDVDYEAPAAEWRKQALDKVKEALASEAQSAQVVQLPVGQAAHTSQYSKQN
CCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCC
PYTATLLTSQKITGRDSGKDVRHIEIDLDGSGLTYQPGDALGVWFENSPELASAILKQVG
CEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHC
LTGDEAVEVDGDSISLQKALVEKYEITSANPQLVTQYAELSGSKKLEKLAADKDKLRQYS
CCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHC
GNTQVIDVLSEKKAKLTAEQLVGLLRRLTPRLYSIASSQSEVDEEVHLTVGVVEYLQGDE
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCCC
TRFGGASSFLSHRLEEGDDVKVFVEHNNNFKLPQDDNAPVIMIGPGTGIAPFRSFVQERD
CCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHC
NRDAEGKNWLFFGDRIFTQDFLYQVEWQKYLKSGIVNQLDVAFSRDQQEKVYVQHRILEH
CCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHH
AAQVWQWLQDGAYIYVCGDATRMAKDVHEALICVVEQHGQKTREEAEQFVNELRKAKRYQ
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
RDVY
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA