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The map label for this gene is leuA [H]

Identifier: 59710902

GI number: 59710902

Start: 301188

End: 302735

Strand: Reverse

Name: leuA [H]

Synonym: VF_0295

Alternate gene names: 59710902

Gene position: 302735-301188 (Counterclockwise)

Preceding gene: 172087653

Following gene: 59710901

Centisome position: 10.45

GC content: 42.12

Gene sequence:

>1548_bases
ATGAGCGATCAAGTCATTATCTTCGACACCACCCTACGTGATGGCGAACAAGCACTATCAGCAAGTCTAACGGTTAAAGA
AAAATTGCAGATTGCTTATGCGCTTGAGCGTTTAGGAGTCGATGTCATTGAAGCCGGTTTTCCGGTGTCATCTCCGGGTG
ATTTTGAATCAGTAAAAACCATTGCCCAGCATATTAAAAACAGTCGAATTTGTGGTCTTTCTCGTGCTGTTGCAAAAGAC
ATTGATGCCGCAGCTGAGGCGCTAAAAGTCGCTGAAGCGTTCCGAATTCACACCTTCATTTCAACCTCTACCGTTCATGT
TCAAGATAAATTACGCCGTAGCTACGATGATGTGGTTGAAATGGGCGTAAAAGCAGTAAAACACGCACGTAAATATACGG
ATGATGTTGAGTTTTCTTGTGAAGATGCAGGCCGTACTCCTATCGATAATCTATGCCGCATGGTTGAAGCCGCTATTGAT
GCAGGAGCCAATACCATCAACATTCCTGACACCGTTGGCTATACCGTTCCAGGTGAATTTGGTGGCATTGTTCAAACCCT
ATTTAACCGTGTTCCTAATATAGATAAAGCCATCATTTCTGTTCACTGTCATGATGATTTGGGTATGTCAGTTGCCAACT
CCATTGCTGCTGTTCAAGCAGGCGCTCGACAAGTTGAAGGAACGATTAATGGTATTGGTGAACGTGCTGGTAATTGCTCT
CTAGAAGAGATCGCCATGATCCTAAAAACACGTTCTGAATATCTAGGTGTACACACCAACCTTAAACACGATGAGATTCA
CCGCACCAGTAAACTAGTGAGTCAGCTTTGTAATATGCCAATTCAAAGTAATAAGGCTATCGTAGGCGCAAACGCTTTTA
GCCATTCATCAGGTATTCACCAAGATGGCATGCTAAAGAATAAAAACACTTACGAAATCATGACACCAGAGTCGATTGGC
CTTAAAAACCAAGCACTAAACCTAACCAGTCGTTCAGGTCGTGCTGCGGTAAAAAGTCATATGGATGAAATGGGTTATAA
AGAAGACGAATACAACCTAGACACCCTTTATGCTGATTTCGTAAAGCTGGCAGATCGTAAAGGACAAGTATTTGATTACG
ATTTAGAAGCACTAATGCATTTTGCTAACCTGCGCGATGAAGATGACTTCTATAAGTTGAACTATCTAAGTGTGCAATCA
GGCAGCGTCATGGCAACCACGAGTATCAAATTACTTTGTGGTGACGAGGAGAAATGCGAAGCCGCTGTCGGTAATGGTCC
TGTAGACGCACTTTATCAATGTATCTACAAACTAACAGGCTACGACATCGTGCTAGATAAGTTTGATTTAACTGCTAAAG
GCGAAGGTGAAGATGGCTTAGGTCAAGCAGATATTATTGCCAATTACAAAGGTCGTAAATATCACGGTACCGGTTTAGCG
ACAGATATTATCGAAGCATCAGGACAAGCACTACTTCACGTAATCAATAGTATTCACCGTGCTGATCAAATTGCTGAAAT
TAAACAGAAAAAAGTAGAAACCGTTTAG

Upstream 100 bases:

>100_bases
AAAGAAACCATAAACCAAATTTTAAAACCCGCTAACCATGGCGGGTTTTTTTATGGATATCCGTCATCAAGGCAAATGAC
AAACACAAGGAAGCAGGCTC

Downstream 100 bases:

>100_bases
AACCTAAAAATTATAAATAAGCGGAGATAAACCTTTCTCCGCTTTAATTTGCCAAATTATATTGAAGATTAAAGGATTAA
CATGACAAACAAAACATACA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSDQVIIFDTTLRDGEQALSASLTVKEKLQIAYALERLGVDVIEAGFPVSSPGDFESVKTIAQHIKNSRICGLSRAVAKD
IDAAAEALKVAEAFRIHTFISTSTVHVQDKLRRSYDDVVEMGVKAVKHARKYTDDVEFSCEDAGRTPIDNLCRMVEAAID
AGANTINIPDTVGYTVPGEFGGIVQTLFNRVPNIDKAIISVHCHDDLGMSVANSIAAVQAGARQVEGTINGIGERAGNCS
LEEIAMILKTRSEYLGVHTNLKHDEIHRTSKLVSQLCNMPIQSNKAIVGANAFSHSSGIHQDGMLKNKNTYEIMTPESIG
LKNQALNLTSRSGRAAVKSHMDEMGYKEDEYNLDTLYADFVKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQS
GSVMATTSIKLLCGDEEKCEAAVGNGPVDALYQCIYKLTGYDIVLDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGLA
TDIIEASGQALLHVINSIHRADQIAEIKQKKVETV

Sequences:

>Translated_515_residues
MSDQVIIFDTTLRDGEQALSASLTVKEKLQIAYALERLGVDVIEAGFPVSSPGDFESVKTIAQHIKNSRICGLSRAVAKD
IDAAAEALKVAEAFRIHTFISTSTVHVQDKLRRSYDDVVEMGVKAVKHARKYTDDVEFSCEDAGRTPIDNLCRMVEAAID
AGANTINIPDTVGYTVPGEFGGIVQTLFNRVPNIDKAIISVHCHDDLGMSVANSIAAVQAGARQVEGTINGIGERAGNCS
LEEIAMILKTRSEYLGVHTNLKHDEIHRTSKLVSQLCNMPIQSNKAIVGANAFSHSSGIHQDGMLKNKNTYEIMTPESIG
LKNQALNLTSRSGRAAVKSHMDEMGYKEDEYNLDTLYADFVKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQS
GSVMATTSIKLLCGDEEKCEAAVGNGPVDALYQCIYKLTGYDIVLDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGLA
TDIIEASGQALLHVINSIHRADQIAEIKQKKVETV
>Mature_514_residues
SDQVIIFDTTLRDGEQALSASLTVKEKLQIAYALERLGVDVIEAGFPVSSPGDFESVKTIAQHIKNSRICGLSRAVAKDI
DAAAEALKVAEAFRIHTFISTSTVHVQDKLRRSYDDVVEMGVKAVKHARKYTDDVEFSCEDAGRTPIDNLCRMVEAAIDA
GANTINIPDTVGYTVPGEFGGIVQTLFNRVPNIDKAIISVHCHDDLGMSVANSIAAVQAGARQVEGTINGIGERAGNCSL
EEIAMILKTRSEYLGVHTNLKHDEIHRTSKLVSQLCNMPIQSNKAIVGANAFSHSSGIHQDGMLKNKNTYEIMTPESIGL
KNQALNLTSRSGRAAVKSHMDEMGYKEDEYNLDTLYADFVKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQSG
SVMATTSIKLLCGDEEKCEAAVGNGPVDALYQCIYKLTGYDIVLDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGLAT
DIIEASGQALLHVINSIHRADQIAEIKQKKVETV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=514, Percent_Identity=68.6770428015564, Blast_Score=744, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320071, Length=360, Percent_Identity=28.3333333333333, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320019, Length=374, Percent_Identity=27.5401069518717, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=572, Percent_Identity=24.4755244755245, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324682, Length=546, Percent_Identity=25.8241758241758, Blast_Score=127, Evalue=3e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56231; Mature: 56100

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQVIIFDTTLRDGEQALSASLTVKEKLQIAYALERLGVDVIEAGFPVSSPGDFESVKT
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHH
IAQHIKNSRICGLSRAVAKDIDAAAEALKVAEAFRIHTFISTSTVHVQDKLRRSYDDVVE
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEHHHHHHHHHHHHHH
MGVKAVKHARKYTDDVEFSCEDAGRTPIDNLCRMVEAAIDAGANTINIPDTVGYTVPGEF
HHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHH
GGIVQTLFNRVPNIDKAIISVHCHDDLGMSVANSIAAVQAGARQVEGTINGIGERAGNCS
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LEEIAMILKTRSEYLGVHTNLKHDEIHRTSKLVSQLCNMPIQSNKAIVGANAFSHSSGIH
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGMLKNKNTYEIMTPESIGLKNQALNLTSRSGRAAVKSHMDEMGYKEDEYNLDTLYADF
CCCCCCCCCEEEEECCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQSGSVMATTSIKLLCGDEEKCE
HHHHCCCCCEEEHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEEEEEEEECCCHHHH
AAVGNGPVDALYQCIYKLTGYDIVLDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGLA
HHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCCEEECCCHH
TDIIEASGQALLHVINSIHRADQIAEIKQKKVETV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDQVIIFDTTLRDGEQALSASLTVKEKLQIAYALERLGVDVIEAGFPVSSPGDFESVKT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHH
IAQHIKNSRICGLSRAVAKDIDAAAEALKVAEAFRIHTFISTSTVHVQDKLRRSYDDVVE
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEHHHHHHHHHHHHHH
MGVKAVKHARKYTDDVEFSCEDAGRTPIDNLCRMVEAAIDAGANTINIPDTVGYTVPGEF
HHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHH
GGIVQTLFNRVPNIDKAIISVHCHDDLGMSVANSIAAVQAGARQVEGTINGIGERAGNCS
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LEEIAMILKTRSEYLGVHTNLKHDEIHRTSKLVSQLCNMPIQSNKAIVGANAFSHSSGIH
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGMLKNKNTYEIMTPESIGLKNQALNLTSRSGRAAVKSHMDEMGYKEDEYNLDTLYADF
CCCCCCCCCEEEEECCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQSGSVMATTSIKLLCGDEEKCE
HHHHCCCCCEEEHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEEEEEEEECCCHHHH
AAVGNGPVDALYQCIYKLTGYDIVLDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGLA
HHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCCEEECCCHH
TDIIEASGQALLHVINSIHRADQIAEIKQKKVETV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA