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The map label for this gene is glnA [H]

Identifier: 59710705

GI number: 59710705

Start: 114336

End: 115745

Strand: Reverse

Name: glnA [H]

Synonym: VF_0098

Alternate gene names: 59710705

Gene position: 115745-114336 (Counterclockwise)

Preceding gene: 59710710

Following gene: 59710704

Centisome position: 3.99

GC content: 43.76

Gene sequence:

>1410_bases
ATGTCAGTAGAAAACGTACTAGCTCTGATTCAAGAGAACGAAGTTAAATTTATCGATTTGCGCTTTACCGATACAAAAGG
TAAAGAGCAACATATCTCAATCCCTTCTCACCAAATTGATGCGGACTTCTTTGAAGAAGGCAAAATGTTTGATGGTTCTT
CAGTTGCAGGCTGGAAAGGCATTAACGAATCTGACATGGTAATGATGCCAGATCCAGCAAGTGCGGTTCTTGACCCATTC
ACAGAAGACTCAACACTAAACATTCGTTGTGACATCCTAGAGCCAGCAACACTTCAAGGCTACGATCGCGATCCACGTTC
AATCGCTAAACGTTCTGAAGAATACATGCGCTCTACTGGTATTGCTGACACAGTTCTAATCGGTCCTGAGCCAGAATTCT
TCCTATTTGATGATGTACGTTTTAACACTGACATGTCTGGTTCTTTCTTCAAGATTGACGACATCGAAGCAGCTTGGAAC
TCAGGTACAGAATACGAAGGCGGTAACAAAGGTCACCGTCCTGGTGTTAAAGGCGGTTACTTCCCAGTAGCACCTGTTGA
TTCATCTCAAGACATCCGTTCTGCTATGTGTTTAATCATGGAAGAAATGGGCCTTGTTGTTGAAGCACACCACCACGAAG
TTGCTACTGCTGGTCAAAATGAGATCGCTACACGCTTTAATACGCTAACAACAAAAGCGGATGAAACTCAAATCTACAAA
TACGTTGTACATAACGTTGCACACGCATTTGGTAAAACAGCGACATTTATGCCTAAGCCACTTGTTGGTGATAACGGTTC
TGGTATGCACGTTCACCAATCTCTAGCAAAAGATGGCGTTAACCTATTTGCTGGCGATAAGTACGGCGGCCTATCTGAAT
TGGCTATCTACTACATTGGCGGTATCATCAAACACGCTCGTGCAATCAACGCATTTGCTAACCCATCAACTAACTCATAC
AAGCGTCTTGTACCTGGTTTTGAAGCACCAGTTATGCTTGCATACTCTGCACGTAACCGTTCAGCATCTATCCGTATCCC
AGTAGTACCAAGCCCGAAAGCTCGTCGTATCGAAGTTCGTTTTGGTGACCCAGCAGCGAACCCATACCTATGCTACTCAG
CAATGCTAATGGCTGGTCTTGACGGTATTAAGAACAAGATCCACCCAGGTGAAGCAATGGATAAAGATCTGTATGACCTT
CCAGCGGAAGAAGCAGCAGAAATCCCAACAGTTGCAGAATCTCTAGATATCGCACTTAAAGCACTAGACGAAGATCGTGA
GTTCCTAACCGCTGGTGGCGTATTCTCTGATGACTTTATCGATTCTTACATCGGTCTTAAATCTCAAGATGTGGAAGCAA
TCAATACAGCAGTTCACCCACTTGAGTTTGAAATGTACTACTCAGTTTAA

Upstream 100 bases:

>100_bases
TGATATTAATGGATTATTACTTTTGGCATGCTTCTAGCAATGTAGTTTATAACAGCTAAGCTGGCCAATATTTATATTAT
AACACCGGAGGTTATCCTAC

Downstream 100 bases:

>100_bases
TATTCACAATTTATTGCTGATTAAAGTAAAGAATTGATTAATTTTTAGGCTCGCCTCACAGGCGAGCCTTTTTTATATTG
GCAAAAAAGGATTTAAAAGC

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSVENVLALIQENEVKFIDLRFTDTKGKEQHISIPSHQIDADFFEEGKMFDGSSVAGWKGINESDMVMMPDPASAVLDPF
TEDSTLNIRCDILEPATLQGYDRDPRSIAKRSEEYMRSTGIADTVLIGPEPEFFLFDDVRFNTDMSGSFFKIDDIEAAWN
SGTEYEGGNKGHRPGVKGGYFPVAPVDSSQDIRSAMCLIMEEMGLVVEAHHHEVATAGQNEIATRFNTLTTKADETQIYK
YVVHNVAHAFGKTATFMPKPLVGDNGSGMHVHQSLAKDGVNLFAGDKYGGLSELAIYYIGGIIKHARAINAFANPSTNSY
KRLVPGFEAPVMLAYSARNRSASIRIPVVPSPKARRIEVRFGDPAANPYLCYSAMLMAGLDGIKNKIHPGEAMDKDLYDL
PAEEAAEIPTVAESLDIALKALDEDREFLTAGGVFSDDFIDSYIGLKSQDVEAINTAVHPLEFEMYYSV

Sequences:

>Translated_469_residues
MSVENVLALIQENEVKFIDLRFTDTKGKEQHISIPSHQIDADFFEEGKMFDGSSVAGWKGINESDMVMMPDPASAVLDPF
TEDSTLNIRCDILEPATLQGYDRDPRSIAKRSEEYMRSTGIADTVLIGPEPEFFLFDDVRFNTDMSGSFFKIDDIEAAWN
SGTEYEGGNKGHRPGVKGGYFPVAPVDSSQDIRSAMCLIMEEMGLVVEAHHHEVATAGQNEIATRFNTLTTKADETQIYK
YVVHNVAHAFGKTATFMPKPLVGDNGSGMHVHQSLAKDGVNLFAGDKYGGLSELAIYYIGGIIKHARAINAFANPSTNSY
KRLVPGFEAPVMLAYSARNRSASIRIPVVPSPKARRIEVRFGDPAANPYLCYSAMLMAGLDGIKNKIHPGEAMDKDLYDL
PAEEAAEIPTVAESLDIALKALDEDREFLTAGGVFSDDFIDSYIGLKSQDVEAINTAVHPLEFEMYYSV
>Mature_468_residues
SVENVLALIQENEVKFIDLRFTDTKGKEQHISIPSHQIDADFFEEGKMFDGSSVAGWKGINESDMVMMPDPASAVLDPFT
EDSTLNIRCDILEPATLQGYDRDPRSIAKRSEEYMRSTGIADTVLIGPEPEFFLFDDVRFNTDMSGSFFKIDDIEAAWNS
GTEYEGGNKGHRPGVKGGYFPVAPVDSSQDIRSAMCLIMEEMGLVVEAHHHEVATAGQNEIATRFNTLTTKADETQIYKY
VVHNVAHAFGKTATFMPKPLVGDNGSGMHVHQSLAKDGVNLFAGDKYGGLSELAIYYIGGIIKHARAINAFANPSTNSYK
RLVPGFEAPVMLAYSARNRSASIRIPVVPSPKARRIEVRFGDPAANPYLCYSAMLMAGLDGIKNKIHPGEAMDKDLYDLP
AEEAAEIPTVAESLDIALKALDEDREFLTAGGVFSDDFIDSYIGLKSQDVEAINTAVHPLEFEMYYSV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=78.6780383795309, Blast_Score=797, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=296, Percent_Identity=27.7027027027027, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51570; Mature: 51438

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVENVLALIQENEVKFIDLRFTDTKGKEQHISIPSHQIDADFFEEGKMFDGSSVAGWKG
CCHHHHHHHHCCCCEEEEEEEEECCCCCCCEECCCCCCCCCHHHHCCCCCCCCCCCCCCC
INESDMVMMPDPASAVLDPFTEDSTLNIRCDILEPATLQGYDRDPRSIAKRSEEYMRSTG
CCCCCEEEECCCHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
IADTVLIGPEPEFFLFDDVRFNTDMSGSFFKIDDIEAAWNSGTEYEGGNKGHRPGVKGGY
CCEEEEECCCCCEEEEECEEECCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDIRSAMCLIMEEMGLVVEAHHHEVATAGQNEIATRFNTLTTKADETQIYK
EECCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHCCCHHHHHHHHHHCCCCCHHHHHH
YVVHNVAHAFGKTATFMPKPLVGDNGSGMHVHQSLAKDGVNLFAGDKYGGLSELAIYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHH
GIIKHARAINAFANPSTNSYKRLVPGFEAPVMLAYSARNRSASIRIPVVPSPKARRIEVR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCEEEEEE
FGDPAANPYLCYSAMLMAGLDGIKNKIHPGEAMDKDLYDLPAEEAAEIPTVAESLDIALK
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCHHHHHCCCHHHHHHHHHHH
ALDEDREFLTAGGVFSDDFIDSYIGLKSQDVEAINTAVHPLEFEMYYSV
HHCCCCCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHCCCEEEEEEECC
>Mature Secondary Structure 
SVENVLALIQENEVKFIDLRFTDTKGKEQHISIPSHQIDADFFEEGKMFDGSSVAGWKG
CHHHHHHHHCCCCEEEEEEEEECCCCCCCEECCCCCCCCCHHHHCCCCCCCCCCCCCCC
INESDMVMMPDPASAVLDPFTEDSTLNIRCDILEPATLQGYDRDPRSIAKRSEEYMRSTG
CCCCCEEEECCCHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
IADTVLIGPEPEFFLFDDVRFNTDMSGSFFKIDDIEAAWNSGTEYEGGNKGHRPGVKGGY
CCEEEEECCCCCEEEEECEEECCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDIRSAMCLIMEEMGLVVEAHHHEVATAGQNEIATRFNTLTTKADETQIYK
EECCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHCCCHHHHHHHHHHCCCCCHHHHHH
YVVHNVAHAFGKTATFMPKPLVGDNGSGMHVHQSLAKDGVNLFAGDKYGGLSELAIYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHH
GIIKHARAINAFANPSTNSYKRLVPGFEAPVMLAYSARNRSASIRIPVVPSPKARRIEVR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCEEEEEE
FGDPAANPYLCYSAMLMAGLDGIKNKIHPGEAMDKDLYDLPAEEAAEIPTVAESLDIALK
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCHHHHHCCCHHHHHHHHHHH
ALDEDREFLTAGGVFSDDFIDSYIGLKSQDVEAINTAVHPLEFEMYYSV
HHCCCCCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]