Click here to switch to the map view.

The map label for this gene is trmH [H]

Identifier: 59710710

GI number: 59710710

Start: 121501

End: 122190

Strand: Reverse

Name: trmH [H]

Synonym: VF_0103

Alternate gene names: 59710710

Gene position: 122190-121501 (Counterclockwise)

Preceding gene: 59710711

Following gene: 59710705

Centisome position: 4.22

GC content: 42.03

Gene sequence:

>690_bases
ATGAATTTAGAACGCTACCAACGAATTACTTCTGTATTAAAAGCTCGTCAAACTGATCTCACTCTTTGTTTAGAAGAAGT
ACATAAAGCCAATAACGTTTCTGCGATTGTTCGTACTGCTGATGCAACAGGCATCCATAAAATTCATGCGGTTTGGCCTA
CAGAGCAGATGCAGATGCTAGGACATACTTCTGCTGGTGCTCGTAATTGGGTTGATGTGGAAACACATAAAAATATTGAA
GATGCTTTTGCTACGCTTAAAGAACAAGGCATGCAGATTCTAGCGACAAATCTATCGGATACAGCTGTAGATTTTCGAGA
AATTGATTACACCAAACCAACTGCCATCATTCTTGGTAGTGAAAAAACAGGCATCAGCAAAAAAGCATTAGCAATGGCTG
ATCAAGACATCATCATTCCTATGGTAGGTATGGTGCAATCACTAAATGTATCGGTTGCAAGTGCGCTTATTTTATATGAA
GCGCAGCGTCAGCGTGATTTAGCGGGTATGTATGATCGTGAAGAGAGTATGCTGCCAAAGGAATACATCAATAAGGTGTT
GTTTGAACGCGGCCACCCTGTTCTTGCGAAAGTAGCCAAACGTAAAGGTCTTGAATTACCACAGCTTGATGAAACTGGGC
AGATCGTTGCTGATGAAGAGTGGTGGGCAAGAATGCAGGCGGCGGATTAG

Upstream 100 bases:

>100_bases
GTCTCTAGCTAGCGCAGCGTATAGTTTCTAGTTCCATTTTGACTACCTCTTTCTCGTCCCTAGAACCTAGTCCCTCCAAC
CTATCTCAAGAATTAAAATT

Downstream 100 bases:

>100_bases
GAGTGAGATCTTAGGATTCAGAGGTAATCGCTGTGATTTCAGCGTATAAATCTGAAAATAGAGACTATAAGAGGTTTCAG
GCTTAAGGTTTCAGGAAGAG

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MNLERYQRITSVLKARQTDLTLCLEEVHKANNVSAIVRTADATGIHKIHAVWPTEQMQMLGHTSAGARNWVDVETHKNIE
DAFATLKEQGMQILATNLSDTAVDFREIDYTKPTAIILGSEKTGISKKALAMADQDIIIPMVGMVQSLNVSVASALILYE
AQRQRDLAGMYDREESMLPKEYINKVLFERGHPVLAKVAKRKGLELPQLDETGQIVADEEWWARMQAAD

Sequences:

>Translated_229_residues
MNLERYQRITSVLKARQTDLTLCLEEVHKANNVSAIVRTADATGIHKIHAVWPTEQMQMLGHTSAGARNWVDVETHKNIE
DAFATLKEQGMQILATNLSDTAVDFREIDYTKPTAIILGSEKTGISKKALAMADQDIIIPMVGMVQSLNVSVASALILYE
AQRQRDLAGMYDREESMLPKEYINKVLFERGHPVLAKVAKRKGLELPQLDETGQIVADEEWWARMQAAD
>Mature_229_residues
MNLERYQRITSVLKARQTDLTLCLEEVHKANNVSAIVRTADATGIHKIHAVWPTEQMQMLGHTSAGARNWVDVETHKNIE
DAFATLKEQGMQILATNLSDTAVDFREIDYTKPTAIILGSEKTGISKKALAMADQDIIIPMVGMVQSLNVSVASALILYE
AQRQRDLAGMYDREESMLPKEYINKVLFERGHPVLAKVAKRKGLELPQLDETGQIVADEEWWARMQAAD

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=228, Percent_Identity=66.2280701754386, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI1790623, Length=148, Percent_Identity=31.7567567567568, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25660; Mature: 25660

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLERYQRITSVLKARQTDLTLCLEEVHKANNVSAIVRTADATGIHKIHAVWPTEQMQML
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCHHHHHHH
GHTSAGARNWVDVETHKNIEDAFATLKEQGMQILATNLSDTAVDFREIDYTKPTAIILGS
HCCCCCCCCCEECCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCEEEEECC
EKTGISKKALAMADQDIIIPMVGMVQSLNVSVASALILYEAQRQRDLAGMYDREESMLPK
CCCCCCHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCH
EYINKVLFERGHPVLAKVAKRKGLELPQLDETGQIVADEEWWARMQAAD
HHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHCCC
>Mature Secondary Structure
MNLERYQRITSVLKARQTDLTLCLEEVHKANNVSAIVRTADATGIHKIHAVWPTEQMQML
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCHHHHHHH
GHTSAGARNWVDVETHKNIEDAFATLKEQGMQILATNLSDTAVDFREIDYTKPTAIILGS
HCCCCCCCCCEECCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCEEEEECC
EKTGISKKALAMADQDIIIPMVGMVQSLNVSVASALILYEAQRQRDLAGMYDREESMLPK
CCCCCCHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCH
EYINKVLFERGHPVLAKVAKRKGLELPQLDETGQIVADEEWWARMQAAD
HHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]