Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 59710609

GI number: 59710609

Start: 817

End: 2184

Strand: Reverse

Name: trmE

Synonym: VF_0002

Alternate gene names: 59710609

Gene position: 2184-817 (Counterclockwise)

Preceding gene: 59710610

Following gene: 59710608

Centisome position: 0.08

GC content: 42.32

Gene sequence:

>1368_bases
ATGACACTACATACAGACACCATTGTTGCACAAGCAACAGCACCAGGCCGAGGCGGCGTTGGCATCATTCGCGTATCAGG
TCCAAAAGCAAAAGACGTTGCGCTTGCTGTTATTGGTAAAGAACTAAAACCTCGTTACGCAGAATATGCGCCATTTAAAG
ATGAAAATGGATTGGAGCTTGATCAGGGGATTGCTCTTTACTTCCCAAACCCACATTCATTTACCGGTGAAGACGTATTA
GAACTTCAAGGTCATGGTGGTCCTGTCGTCATGGATATGCTAATCAAACGCATTCTAAAGATTGATGGTATCCGTACAGC
ACGTCCAGGAGAGTTCTCAGAAAGAGCTTTCTTAAACGACAAAATGGATTTAGCTCAAGCAGAAGCGATTGCTGATTTAA
TTGATGCAAGCTCTGAAGAAGCGGCTAAATCAGCCTTAAATTCATTACAAGGTGTTTTCTCAAATAAGATTAATACCTTA
GTTGAATCGCTTATCTATCTACGTATTTACGTTGAAGCAGCAATCGACTTCCCTGAAGAGGAAATCGATTTTCTTGCTGA
TGGTAAAGTCAGTGGTGATTTACAAGGCATCATTGATAACCTTAATGCGGTTCGTAAAGAAGCGAACCAAGGTGCCATCA
TGCGTGAAGGTATGAAGGTTGTTATCGCAGGTCGTCCAAATGCAGGTAAATCTAGCCTTCTTAACGCATTGTCAGGTAAA
GATACCGCTATCGTTACCGACATAGCTGGTACAACCCGTGACGTGCTTAGAGAGCACATTCATATTGATGGTATGCCATT
GCATATTATTGATACTGCAGGCCTTCGTGAAGCGTCTGATGAGGTTGAACGTATTGGTATTGAAAGAGCTTGGGATGAAA
TAGCCCAAGCTGACCGCGTTTTATTTATGGTTGATGGAACAACAACAGACGCAACAGACCCTAAATTAATTTGGCCTGAA
TTCCTTGATCGTCTACCAAAAAACATGGGAATGACAGTGATCAGAAACAAGGCGGATCAAACCGGTGAAGATCTCGGTAT
TTGCCATGTTAATGATCCTACATTGATTCGCCTATCAGCAAAAACAGGTGAAGGGGTTGATGCACTGCGTAACCATTTAA
AAGAGTGCATGGGCTTCTCGGGTAACCAAGAAGGTGGCTTTATGGCTCGTCGACGCCATCTAGAAGCCTTAGAAAAAGCA
GCTCAACACCTCGATATAGGTCAGCAACAATTAGAAGGTTACATGGCGGGAGAAATCCTTGCAGAAGAACTTCGTATTGC
ACAACAACATTTAAGTGAAATCACCGGTGAATTTACCTCTGATGATTTACTTGGACGTATATTTACTTCATTCTGTATTG
GTAAATAA

Upstream 100 bases:

>100_bases
GTAACCTTTCACTCTTGAAGTAAAACAAAGGCGGCCATTGGTCGCCTTTTTTGTTCAATGAGCATACAATATCAGTAAGC
AATAGACATAAAGAATAATC

Downstream 100 bases:

>100_bases
AACCAATATTAATAAGATTAATAATGGGCCTATGTAGGCCCATTTTTTATATTTTTATAGGATAAATTCATGAAAAAAGT
ATCTATTATTACAGGAAGCA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTLHTDTIVAQATAPGRGGVGIIRVSGPKAKDVALAVIGKELKPRYAEYAPFKDENGLELDQGIALYFPNPHSFTGEDVL
ELQGHGGPVVMDMLIKRILKIDGIRTARPGEFSERAFLNDKMDLAQAEAIADLIDASSEEAAKSALNSLQGVFSNKINTL
VESLIYLRIYVEAAIDFPEEEIDFLADGKVSGDLQGIIDNLNAVRKEANQGAIMREGMKVVIAGRPNAGKSSLLNALSGK
DTAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWDEIAQADRVLFMVDGTTTDATDPKLIWPE
FLDRLPKNMGMTVIRNKADQTGEDLGICHVNDPTLIRLSAKTGEGVDALRNHLKECMGFSGNQEGGFMARRRHLEALEKA
AQHLDIGQQQLEGYMAGEILAEELRIAQQHLSEITGEFTSDDLLGRIFTSFCIGK

Sequences:

>Translated_455_residues
MTLHTDTIVAQATAPGRGGVGIIRVSGPKAKDVALAVIGKELKPRYAEYAPFKDENGLELDQGIALYFPNPHSFTGEDVL
ELQGHGGPVVMDMLIKRILKIDGIRTARPGEFSERAFLNDKMDLAQAEAIADLIDASSEEAAKSALNSLQGVFSNKINTL
VESLIYLRIYVEAAIDFPEEEIDFLADGKVSGDLQGIIDNLNAVRKEANQGAIMREGMKVVIAGRPNAGKSSLLNALSGK
DTAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWDEIAQADRVLFMVDGTTTDATDPKLIWPE
FLDRLPKNMGMTVIRNKADQTGEDLGICHVNDPTLIRLSAKTGEGVDALRNHLKECMGFSGNQEGGFMARRRHLEALEKA
AQHLDIGQQQLEGYMAGEILAEELRIAQQHLSEITGEFTSDDLLGRIFTSFCIGK
>Mature_454_residues
TLHTDTIVAQATAPGRGGVGIIRVSGPKAKDVALAVIGKELKPRYAEYAPFKDENGLELDQGIALYFPNPHSFTGEDVLE
LQGHGGPVVMDMLIKRILKIDGIRTARPGEFSERAFLNDKMDLAQAEAIADLIDASSEEAAKSALNSLQGVFSNKINTLV
ESLIYLRIYVEAAIDFPEEEIDFLADGKVSGDLQGIIDNLNAVRKEANQGAIMREGMKVVIAGRPNAGKSSLLNALSGKD
TAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWDEIAQADRVLFMVDGTTTDATDPKLIWPEF
LDRLPKNMGMTVIRNKADQTGEDLGICHVNDPTLIRLSAKTGEGVDALRNHLKECMGFSGNQEGGFMARRRHLEALEKAA
QHLDIGQQQLEGYMAGEILAEELRIAQQHLSEITGEFTSDDLLGRIFTSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=469, Percent_Identity=34.9680170575693, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=32.8657314629259, Blast_Score=222, Evalue=7e-58,
Organism=Homo sapiens, GI193082993, Length=459, Percent_Identity=33.9869281045752, Blast_Score=221, Evalue=1e-57,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=76.940133037694, Blast_Score=712, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=194, Percent_Identity=29.3814432989691, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=459, Percent_Identity=29.6296296296296, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6323665, Length=504, Percent_Identity=32.1428571428571, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI45550813, Length=495, Percent_Identity=33.5353535353535, Blast_Score=223, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_VIBF1 (Q5E8Z9)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203385.1
- HSSP:   P25522
- ProteinModelPortal:   Q5E8Z9
- SMR:   Q5E8Z9
- STRING:   Q5E8Z9
- GeneID:   3279794
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0002
- NMPDR:   fig|312309.3.peg.2
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q5E8Z9
- ProtClustDB:   PRK05291
- BioCyc:   VFIS312309:VF0002-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49608; Mature: 49477

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: BINDING 24-24 BINDING 81-81 BINDING 121-121 BINDING 455-455

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLHTDTIVAQATAPGRGGVGIIRVSGPKAKDVALAVIGKELKPRYAEYAPFKDENGLEL
CCCCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHCHHHCCCHHHCCCCCCCCCCCC
DQGIALYFPNPHSFTGEDVLELQGHGGPVVMDMLIKRILKIDGIRTARPGEFSERAFLND
CCCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCC
KMDLAQAEAIADLIDASSEEAAKSALNSLQGVFSNKINTLVESLIYLRIYVEAAIDFPEE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EIDFLADGKVSGDLQGIIDNLNAVRKEANQGAIMREGMKVVIAGRPNAGKSSLLNALSGK
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCHHHHHHHHCCC
DTAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWDEIAQADRV
CCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCE
LFMVDGTTTDATDPKLIWPEFLDRLPKNMGMTVIRNKADQTGEDLGICHVNDPTLIRLSA
EEEEECCCCCCCCCCEECHHHHHHCHHHCCCHHEECCCCCCCCCCEEEECCCCEEEEEEC
KTGEGVDALRNHLKECMGFSGNQEGGFMARRRHLEALEKAAQHLDIGQQQLEGYMAGEIL
CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AEELRIAQQHLSEITGEFTSDDLLGRIFTSFCIGK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLHTDTIVAQATAPGRGGVGIIRVSGPKAKDVALAVIGKELKPRYAEYAPFKDENGLEL
CCCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHCHHHCCCHHHCCCCCCCCCCCC
DQGIALYFPNPHSFTGEDVLELQGHGGPVVMDMLIKRILKIDGIRTARPGEFSERAFLND
CCCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCC
KMDLAQAEAIADLIDASSEEAAKSALNSLQGVFSNKINTLVESLIYLRIYVEAAIDFPEE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EIDFLADGKVSGDLQGIIDNLNAVRKEANQGAIMREGMKVVIAGRPNAGKSSLLNALSGK
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCHHHHHHHHCCC
DTAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWDEIAQADRV
CCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCE
LFMVDGTTTDATDPKLIWPEFLDRLPKNMGMTVIRNKADQTGEDLGICHVNDPTLIRLSA
EEEEECCCCCCCCCCEECHHHHHHCHHHCCCHHEECCCCCCCCCCEEEECCCCEEEEEEC
KTGEGVDALRNHLKECMGFSGNQEGGFMARRRHLEALEKAAQHLDIGQQQLEGYMAGEIL
CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AEELRIAQQHLSEITGEFTSDDLLGRIFTSFCIGK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA