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The map label for this gene is rpoH [H]

Identifier: 172087778

GI number: 172087778

Start: 2749830

End: 2750690

Strand: Reverse

Name: rpoH [H]

Synonym: VF_2450

Alternate gene names: 172087778

Gene position: 2750690-2749830 (Counterclockwise)

Preceding gene: 59713058

Following gene: 59713055

Centisome position: 94.93

GC content: 41.0

Gene sequence:

>861_bases
ATGTCGAAAGAAATGTATTCAATGGCTGTCGTTTCACAAGATAGTATCGATAGCTATCTACGATCTGTTAATAGTTACCC
AATGTTAACTGTGGCAGAGGAACGTGAGCTAGCGGAGCGTTTACACTACAAAGAAGAAATAGAGGCAGCGAAAGAGCTGA
TCCTATCTCATCTTCGTTTCGTCGTGCATGTTGCCCGTGGTTATTCTGGTTACGGCCTACCTTTAGCGGATCTTATTCAA
GAAGGTAACGTAGGCTTAATGAAAGCAGTAAAACGTTTTAACCCTGAAGTGGGTGTTCGTTTGGTCTCTTTTGCTGTGCA
CTGGATTAAAGCTGAAATTCATGAATACGTATTAAGAAACTGGCGTATCGTGAAAGTAGCAACAACCAAAGCGCAACGTA
AATTGTTTTTTAATTTAAGAAAGAATAAAAAACGTTTAGGTTGGTTTAACAACGGTGAAGTTGAAGCCGTTGCAAAAGAT
CTTGGTGTTGCACCTTCTGAAGTTCGAGAGATGGAAGCGCGACTTGCAGCTCAAGACTCAACGTTTGAAGCTCCAGCTGA
AGATGATGATCGTGATATGGCGTTTACGGCACCAGTTTACTATTTAGAAGATAAGCAATCTGATGTTGCATACTCTCTAG
AAGAAGACAACTGGGAAGATTATACAAATAATCGTTTAGCAAATGCGATTGCGACGCTAGATGAACGTAGTCAACATATT
GTTCAATCTCGTTGGTTAGCGGATGAGAAAGCGACACTGCAAGAGTTAGCAGATACTTATCAAGTATCGGCGGAGCGAAT
TCGTCAACTTGAGAAGAATGCGATGAAAAAATTGAAGGACGCCGTTGGTGATGTTCTTTAA

Upstream 100 bases:

>100_bases
AGTGTGGATAATTTTCCGCCTTTCACTTGCAGTCTGTTTATTTTAAGTACATTATTGCAAGGTTAAGTTAAGTAATGCCA
AGATATAAATGGGGATTTGA

Downstream 100 bases:

>100_bases
TTAAGATAAGAATTTTAAAAAAGGCTGAACTTGAAAGAGTTCAGCCTTTTTTTATACCTGAAGTAATCGAACGACTGATT
AATCGGATTGATATCATTAA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSKEMYSMAVVSQDSIDSYLRSVNSYPMLTVAEERELAERLHYKEEIEAAKELILSHLRFVVHVARGYSGYGLPLADLIQ
EGNVGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKNKKRLGWFNNGEVEAVAKD
LGVAPSEVREMEARLAAQDSTFEAPAEDDDRDMAFTAPVYYLEDKQSDVAYSLEEDNWEDYTNNRLANAIATLDERSQHI
VQSRWLADEKATLQELADTYQVSAERIRQLEKNAMKKLKDAVGDVL

Sequences:

>Translated_286_residues
MSKEMYSMAVVSQDSIDSYLRSVNSYPMLTVAEERELAERLHYKEEIEAAKELILSHLRFVVHVARGYSGYGLPLADLIQ
EGNVGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKNKKRLGWFNNGEVEAVAKD
LGVAPSEVREMEARLAAQDSTFEAPAEDDDRDMAFTAPVYYLEDKQSDVAYSLEEDNWEDYTNNRLANAIATLDERSQHI
VQSRWLADEKATLQELADTYQVSAERIRQLEKNAMKKLKDAVGDVL
>Mature_285_residues
SKEMYSMAVVSQDSIDSYLRSVNSYPMLTVAEERELAERLHYKEEIEAAKELILSHLRFVVHVARGYSGYGLPLADLIQE
GNVGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKNKKRLGWFNNGEVEAVAKDL
GVAPSEVREMEARLAAQDSTFEAPAEDDDRDMAFTAPVYYLEDKQSDVAYSLEEDNWEDYTNNRLANAIATLDERSQHIV
QSRWLADEKATLQELADTYQVSAERIRQLEKNAMKKLKDAVGDVL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=283, Percent_Identity=67.8445229681979, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1789098, Length=272, Percent_Identity=26.1029411764706, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789448, Length=270, Percent_Identity=27.037037037037, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32792; Mature: 32661

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEMYSMAVVSQDSIDSYLRSVNSYPMLTVAEERELAERLHYKEEIEAAKELILSHLRF
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVHVARGYSGYGLPLADLIQEGNVGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKNKKRLGWFNNGEVEAVAKDLGVAPSEVREMEARLAAQDS
CEEEEEEHHHHHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCC
TFEAPAEDDDRDMAFTAPVYYLEDKQSDVAYSLEEDNWEDYTNNRLANAIATLDERSQHI
CCCCCCCCCCCCEEEECCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
VQSRWLADEKATLQELADTYQVSAERIRQLEKNAMKKLKDAVGDVL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKEMYSMAVVSQDSIDSYLRSVNSYPMLTVAEERELAERLHYKEEIEAAKELILSHLRF
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVHVARGYSGYGLPLADLIQEGNVGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKNKKRLGWFNNGEVEAVAKDLGVAPSEVREMEARLAAQDS
CEEEEEEHHHHHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCC
TFEAPAEDDDRDMAFTAPVYYLEDKQSDVAYSLEEDNWEDYTNNRLANAIATLDERSQHI
CCCCCCCCCCCCEEEECCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
VQSRWLADEKATLQELADTYQVSAERIRQLEKNAMKKLKDAVGDVL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA