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The map label for this gene is glpE

Identifier: 59713055

GI number: 59713055

Start: 2749027

End: 2749350

Strand: Reverse

Name: glpE

Synonym: VF_2448

Alternate gene names: 59713055

Gene position: 2749350-2749027 (Counterclockwise)

Preceding gene: 172087778

Following gene: 59713054

Centisome position: 94.89

GC content: 37.35

Gene sequence:

>324_bases
ATGGATCAGTTTCAACATATATCAGTAGTAGACGCTCAAGAAAAATTACAACAGCAAGATCTTAATGCTGTATTGGTAGA
TATTAGGGATCCACAATCCTTCATTAGAGGGCATGTAGAAAATGCATTTCATTTAACTAATGACACGATCGTTGAGTTAA
TGAATGAGGTTGATTTTGAACAACCGGTTTTGGTTATGTGCTATCACGGACATAGTAGCCAAGGTGCAGCTCAGTATTTA
GTGAATCAAGGCTATGAAGAAGTTTATAGTGTTGATGGTGGATTTGAAGGTTGGCATAAAGCAGGGTTACCTGTGGAGAA
ATAA

Upstream 100 bases:

>100_bases
ATAACTATGTGGATAACTTTGTTTAACCCTTACGTACTCTGTGTTTTTCTTGTGGTTAATCGAGGGTACAATAAATAAAA
AACAGAGAGAAAGGCTCATA

Downstream 100 bases:

>100_bases
AAGCATATGCAAAGATTAATCGCATTATCAAACCCTAGAGCAGGACAAGCATTCATTGATTATATGGCATCAAGACATAT
CGATATCAGAATGATGCCAG

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 107; Mature: 107

Protein sequence:

>107_residues
MDQFQHISVVDAQEKLQQQDLNAVLVDIRDPQSFIRGHVENAFHLTNDTIVELMNEVDFEQPVLVMCYHGHSSQGAAQYL
VNQGYEEVYSVDGGFEGWHKAGLPVEK

Sequences:

>Translated_107_residues
MDQFQHISVVDAQEKLQQQDLNAVLVDIRDPQSFIRGHVENAFHLTNDTIVELMNEVDFEQPVLVMCYHGHSSQGAAQYL
VNQGYEEVYSVDGGFEGWHKAGLPVEK
>Mature_107_residues
MDQFQHISVVDAQEKLQQQDLNAVLVDIRDPQSFIRGHVENAFHLTNDTIVELMNEVDFEQPVLVMCYHGHSSQGAAQYL
VNQGYEEVYSVDGGFEGWHKAGLPVEK

Specific function: Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain

Homologues:

Organism=Escherichia coli, GI1789831, Length=106, Percent_Identity=62.2641509433962, Blast_Score=138, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLPE_VIBF1 (Q5E203)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205831.1
- HSSP:   P0A6V5
- ProteinModelPortal:   Q5E203
- SMR:   Q5E203
- STRING:   Q5E203
- GeneID:   3279676
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2448
- NMPDR:   fig|312309.3.peg.2448
- eggNOG:   COG0607
- HOGENOM:   HBG743107
- OMA:   FSAWHEA
- PhylomeDB:   Q5E203
- ProtClustDB:   PRK00162
- BioCyc:   VFIS312309:VF2448-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01009
- InterPro:   IPR001763
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: =2.8.1.1

Molecular weight: Translated: 12131; Mature: 12131

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS50206 RHODANESE_3

Important sites: ACT_SITE 67-67

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQFQHISVVDAQEKLQQQDLNAVLVDIRDPQSFIRGHVENAFHLTNDTIVELMNEVDFE
CCCCCCCEEECHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEECCHHHHHHHHHHCCCC
QPVLVMCYHGHSSQGAAQYLVNQGYEEVYSVDGGFEGWHKAGLPVEK
CCEEEEEECCCCCCHHHHHHHHCCHHHHHHCCCCCCCHHCCCCCCCC
>Mature Secondary Structure
MDQFQHISVVDAQEKLQQQDLNAVLVDIRDPQSFIRGHVENAFHLTNDTIVELMNEVDFE
CCCCCCCEEECHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEECCHHHHHHHHHHCCCC
QPVLVMCYHGHSSQGAAQYLVNQGYEEVYSVDGGFEGWHKAGLPVEK
CCEEEEEECCCCCCHHHHHHHHCCHHHHHHCCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA