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The map label for this gene is dusB [H]

Identifier: 172087771

GI number: 172087771

Start: 2678316

End: 2679365

Strand: Direct

Name: dusB [H]

Synonym: VF_2389

Alternate gene names: 172087771

Gene position: 2678316-2679365 (Clockwise)

Preceding gene: 59712995

Following gene: 59712998

Centisome position: 92.43

GC content: 45.14

Gene sequence:

>1050_bases
ATGCTCAAAATTTGCTCTTTTCAGGCGGGGAAAAAATGCGTAAAATGCGCGCCCTTGCTGACACAAACGTGTGTGGATGT
TTTGCAGATAGGTCAATATACATTAAAAAATAATCTTATCGTTGCACCAATGGCTGGTGTTACTGATAGGCCCTTTCGTG
AGTTGTGTATGCGTTATGGCGCAGGCATGGCTGTGAGCGAAATGATGTCTTCTAATCCGAAACTGTGGAAAACATCTAAG
TCCCAAAACCGCATGGTACATGAAGGTGAATCTGGTATTCGCTCGGTACAAATTGCAGGGGCTGATCCAAAGTTAATGGC
GGAAGCAGCGCAACACTGTGTTGAAACCGGTGCGCAAATCATCGATATCAACATGGGATGCCCTGCAAAAAAAGTGAATA
AGAAGCTAGCAGGCTCAGCACTATTACAGCGCCCAGATCTTATTGAAGAGATCTTACTGGCTGTAGTTAATGCTGTTGAT
GTTCCTGTCACTCTGAAAACTCGCACAGGCTGGGATCCAGACAATAGAAATTGTGTTCAGGTTGCAAAAATGGCTGAACA
ATGTGGCATCCAAGCTTTAGCGCTTCATGGGCGTACCAGAGCTTGTATGTACAAAGGTGAAGCTGAGTACGACAACATTA
AAGCGGTAAAAGAAGCGATTTCTATTCCGGTTATAGCGAACGGTGATATCGACTCGCCGGAGAAGGCTCGTTTTGTGCTT
GATTACACAGGCGCAGACGCTTTGATGATCGGCCGTCCTGCACAAGGTAGGCCATGGATTTTTAAAGAAATCCAACATTA
CTTAGAAACGGGCGAACTCATGCCACCTCTTCCAATTGAAGAAGTGAAGGCCACTTTACTTGGCCATGTTCATGCGCTTC
ACCAATTTTATGGTGAGTTTTTAGGTCCTCGCATTGCACGTAAGCATGTGGGTTGGTACCTAAAAGAGCATGAGCAAGCA
AATGATTTCCGCCGCAGCTTTAACGCGATAGAGGATGCTTCATTGCAACTCTCTCAACTTGAAAGCTATTTCGACCACGT
TGCATCATAA

Upstream 100 bases:

>100_bases
TGCGCTTTTTTGATAAAACTTCAATGCCACAATACTAAATTTGAGGCATGAAACCGCCTAATTGACTGAATTATAGACAA
TTTACAAAAAGAAAATGTAA

Downstream 100 bases:

>100_bases
TAAAAGAAGAGCTAAACAGAATATGTTCGATCAATCTATGACTTCAGAAGCATTAACAGTTACAACAGTTACAGCACAAG
ACCAAATTACTCAGAAACCA

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MLKICSFQAGKKCVKCAPLLTQTCVDVLQIGQYTLKNNLIVAPMAGVTDRPFRELCMRYGAGMAVSEMMSSNPKLWKTSK
SQNRMVHEGESGIRSVQIAGADPKLMAEAAQHCVETGAQIIDINMGCPAKKVNKKLAGSALLQRPDLIEEILLAVVNAVD
VPVTLKTRTGWDPDNRNCVQVAKMAEQCGIQALALHGRTRACMYKGEAEYDNIKAVKEAISIPVIANGDIDSPEKARFVL
DYTGADALMIGRPAQGRPWIFKEIQHYLETGELMPPLPIEEVKATLLGHVHALHQFYGEFLGPRIARKHVGWYLKEHEQA
NDFRRSFNAIEDASLQLSQLESYFDHVAS

Sequences:

>Translated_349_residues
MLKICSFQAGKKCVKCAPLLTQTCVDVLQIGQYTLKNNLIVAPMAGVTDRPFRELCMRYGAGMAVSEMMSSNPKLWKTSK
SQNRMVHEGESGIRSVQIAGADPKLMAEAAQHCVETGAQIIDINMGCPAKKVNKKLAGSALLQRPDLIEEILLAVVNAVD
VPVTLKTRTGWDPDNRNCVQVAKMAEQCGIQALALHGRTRACMYKGEAEYDNIKAVKEAISIPVIANGDIDSPEKARFVL
DYTGADALMIGRPAQGRPWIFKEIQHYLETGELMPPLPIEEVKATLLGHVHALHQFYGEFLGPRIARKHVGWYLKEHEQA
NDFRRSFNAIEDASLQLSQLESYFDHVAS
>Mature_349_residues
MLKICSFQAGKKCVKCAPLLTQTCVDVLQIGQYTLKNNLIVAPMAGVTDRPFRELCMRYGAGMAVSEMMSSNPKLWKTSK
SQNRMVHEGESGIRSVQIAGADPKLMAEAAQHCVETGAQIIDINMGCPAKKVNKKLAGSALLQRPDLIEEILLAVVNAVD
VPVTLKTRTGWDPDNRNCVQVAKMAEQCGIQALALHGRTRACMYKGEAEYDNIKAVKEAISIPVIANGDIDSPEKARFVL
DYTGADALMIGRPAQGRPWIFKEIQHYLETGELMPPLPIEEVKATLLGHVHALHQFYGEFLGPRIARKHVGWYLKEHEQA
NDFRRSFNAIEDASLQLSQLESYFDHVAS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=235, Percent_Identity=32.7659574468085, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI239788462, Length=256, Percent_Identity=31.640625, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=32.15859030837, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI8923374, Length=253, Percent_Identity=32.8063241106719, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI40807366, Length=273, Percent_Identity=29.3040293040293, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=70.404984423676, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI1788462, Length=307, Percent_Identity=29.6416938110749, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI145693211, Length=327, Percent_Identity=23.8532110091743, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=251, Percent_Identity=31.4741035856574, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=32.4324324324324, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=265, Percent_Identity=30.188679245283, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=263, Percent_Identity=28.1368821292776, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=32.9004329004329, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323433, Length=262, Percent_Identity=29.0076335877863, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=229, Percent_Identity=26.6375545851528, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24585320, Length=275, Percent_Identity=31.2727272727273, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24580595, Length=268, Percent_Identity=30.5970149253731, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19920448, Length=268, Percent_Identity=30.5970149253731, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45549423, Length=246, Percent_Identity=30.4878048780488, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=27.3127753303965, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38603; Mature: 38603

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKICSFQAGKKCVKCAPLLTQTCVDVLQIGQYTLKNNLIVAPMAGVTDRPFRELCMRYG
CCEECCCHHCHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCHHHHHHHHHC
AGMAVSEMMSSNPKLWKTSKSQNRMVHEGESGIRSVQIAGADPKLMAEAAQHCVETGAQI
CCHHHHHHHHCCCCEEECCCCCCCCHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEE
IDINMGCPAKKVNKKLAGSALLQRPDLIEEILLAVVNAVDVPVTLKTRTGWDPDNRNCVQ
EEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHH
VAKMAEQCGIQALALHGRTRACMYKGEAEYDNIKAVKEAISIPVIANGDIDSPEKARFVL
HHHHHHHCCCEEEECCCCCCEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEE
DYTGADALMIGRPAQGRPWIFKEIQHYLETGELMPPLPIEEVKATLLGHVHALHQFYGEF
EECCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LGPRIARKHVGWYLKEHEQANDFRRSFNAIEDASLQLSQLESYFDHVAS
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKICSFQAGKKCVKCAPLLTQTCVDVLQIGQYTLKNNLIVAPMAGVTDRPFRELCMRYG
CCEECCCHHCHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCHHHHHHHHHC
AGMAVSEMMSSNPKLWKTSKSQNRMVHEGESGIRSVQIAGADPKLMAEAAQHCVETGAQI
CCHHHHHHHHCCCCEEECCCCCCCCHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEE
IDINMGCPAKKVNKKLAGSALLQRPDLIEEILLAVVNAVDVPVTLKTRTGWDPDNRNCVQ
EEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHH
VAKMAEQCGIQALALHGRTRACMYKGEAEYDNIKAVKEAISIPVIANGDIDSPEKARFVL
HHHHHHHCCCEEEECCCCCCEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEE
DYTGADALMIGRPAQGRPWIFKEIQHYLETGELMPPLPIEEVKATLLGHVHALHQFYGEF
EECCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LGPRIARKHVGWYLKEHEQANDFRRSFNAIEDASLQLSQLESYFDHVAS
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]