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The map label for this gene is fis [H]

Identifier: 59712998

GI number: 59712998

Start: 2679310

End: 2679684

Strand: Direct

Name: fis [H]

Synonym: VF_2391

Alternate gene names: 59712998

Gene position: 2679310-2679684 (Clockwise)

Preceding gene: 172087771

Following gene: 59713001

Centisome position: 92.47

GC content: 38.4

Gene sequence:

>375_bases
ATGCTTCATTGCAACTCTCTCAACTTGAAAGCTATTTCGACCACGTTGCATCATAATAAAAGAAGAGCTAAACAGAATAT
GTTCGATCAATCTATGACTTCAGAAGCATTAACAGTTACAACAGTTACAGCACAAGACCAAATTACTCAGAAACCACTAC
GTGACTCTGTTAAGGCTTCGCTGAAAAACTACTTAGGCCAACTAAACGGTCAAGAAGTTAACGACTTATATGAATTAGTA
TTAGCTGAAGTAGAGCAACCTCTACTAGACATGATCATGCAGCATACTCGCGGCAACCAAACAAAAGCAGCAAACATGAT
GGGTATCAACCGTGGTACTCTTCGTAAGAAACTTAAAAAATACGGCATGAACTAA

Upstream 100 bases:

>100_bases
GAGTTTTTAGGTCCTCGCATTGCACGTAAGCATGTGGGTTGGTACCTAAAAGAGCATGAGCAAGCAAATGATTTCCGCCG
CAGCTTTAACGCGATAGAGG

Downstream 100 bases:

>100_bases
TCCACGCCTTATTTGAAAAATTTAAAGCAGTGTTAAGCAATTAACGCTGCTTTTTTATATCTGCCAAATAAATAATACCA
ATCGTAGTAAATAACTGATC

Product: DNA-binding protein Fis

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
MLHCNSLNLKAISTTLHHNKRRAKQNMFDQSMTSEALTVTTVTAQDQITQKPLRDSVKASLKNYLGQLNGQEVNDLYELV
LAEVEQPLLDMIMQHTRGNQTKAANMMGINRGTLRKKLKKYGMN

Sequences:

>Translated_124_residues
MLHCNSLNLKAISTTLHHNKRRAKQNMFDQSMTSEALTVTTVTAQDQITQKPLRDSVKASLKNYLGQLNGQEVNDLYELV
LAEVEQPLLDMIMQHTRGNQTKAANMMGINRGTLRKKLKKYGMN
>Mature_124_residues
MLHCNSLNLKAISTTLHHNKRRAKQNMFDQSMTSEALTVTTVTAQDQITQKPLRDSVKASLKNYLGQLNGQEVNDLYELV
LAEVEQPLLDMIMQHTRGNQTKAANMMGINRGTLRKKLKKYGMN

Specific function: Activates ribosomal RNA transcription. Plays a direct role in upstream activation of rRNA promoters [H]

COG id: COG2901

COG function: function code KL; Factor for inversion stimulation Fis, transcriptional activator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transcriptional regulatory fis family [H]

Homologues:

Organism=Escherichia coli, GI1789661, Length=98, Percent_Identity=80.6122448979592, Blast_Score=170, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005412
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197 [H]

Pfam domain/function: PF02954 HTH_8 [H]

EC number: NA

Molecular weight: Translated: 14059; Mature: 14059

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
6.5 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHCNSLNLKAISTTLHHNKRRAKQNMFDQSMTSEALTVTTVTAQDQITQKPLRDSVKAS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
LKNYLGQLNGQEVNDLYELVLAEVEQPLLDMIMQHTRGNQTKAANMMGINRGTLRKKLKK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHH
YGMN
HCCC
>Mature Secondary Structure
MLHCNSLNLKAISTTLHHNKRRAKQNMFDQSMTSEALTVTTVTAQDQITQKPLRDSVKAS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
LKNYLGQLNGQEVNDLYELVLAEVEQPLLDMIMQHTRGNQTKAANMMGINRGTLRKKLKK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHH
YGMN
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA