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The map label for this gene is amtB [C]

Identifier: 172087755

GI number: 172087755

Start: 2409535

End: 2410764

Strand: Reverse

Name: amtB [C]

Synonym: VF_2152

Alternate gene names: 172087755

Gene position: 2410764-2409535 (Counterclockwise)

Preceding gene: 59712760

Following gene: 59712758

Centisome position: 83.2

GC content: 41.71

Gene sequence:

>1230_bases
ATGGAACTTTCAACAACAGTATCAGAACTTCGTTATGCGTTAGATACCTTCTTCTTTTTGATGTCAGGTGCATTAGTTAT
GTGGATGGCTGCAGGGTTTGCCATGTTAGAAGCTGGTTTAGTTCGTTCAAAAAATACAACAGAGATCCTAACTAAAAATA
TCTGTTTATATGCAATCGCTTGTACTACATATTTAGTCGTTGGTTATAACATTATGTATGTTGATAACGGGTCTGGTGGT
TTTTTACCCTCTATAGGAACCTTAATTGGTACACAGGCTGAAGGTGCTGATCATTCATTAGAATCAGACTTTTTCTTCCA
AGTTGTGTTTGTAGCTACTGCAATGTCAGTGGTTTCAGGTGCGGTTGCTGAGCGTATGAAGCTATGGTCATTCCTTATTT
TTTCAGCGATTTTAACCGCTTTTATTTATCCAATGGAAGGTTATTGGACATGGGGTGGTGGCTTCCTATCAGAAGCAGGA
TTCAGTGACTTTGCGGGCTCTGGTATCGTTCATATGGCTGGTGCATCTGCGGCGCTTGCAGGCGTTCTATTACTAGGGGC
TCGTAAAGGTAAGTACGGTAAGAAAGGTCAAATATATCCAATTCCAGGTTCAAACATGCCTTTGGCTACACTGGGTACTT
TTATTTTATGGTTTGGTTGGTTTGGTTTTAATGGTGGTTCTCAGTTAATGGTTTCTGACTTTGAGAATGCAACTGCGGTT
GGCCAGATCTTTTTGAATACGAATGCAGCTGCGGCGGCAGGAGCAATTACGGCATTATTTGTATGTAAGAGCACATGGGG
TAAAGCTGATTTAACCATGGTATTAAATGGGGCATTAGCAGGTTTAGTTGCTATCACTGCAGATCCGTTATCACCGTCAC
CATTAGCTGCTGTAAGTGTTGGTGCAGTCGCTGGAGCTCTGGTTGTATTTAGTATTGTTGGTTTTGATAAAATTAAAATT
GATGATCCTGTAGGTGCTATTTCTGTTCATGGTGTGTGTGGCTTCTTTGGTTTAATGGTGGTTCCTTTGAGTAACTCTGA
TGCCACATTTGGAGCGCAACTACTTGGTGCTGTCGTGATCTTCGCATGGGTATTTGGCGCAAGTTTAGTTGTATGGGCGG
TATTGAAAGCGACAGTCGGCATTCGAGTAACAGAGGAAGAGGAGCTTGAAGGTATGGATGTACATGATTGTGGTATCGAT
GCTTACCCAGAGTTTGTAAGTGTAAAATAG

Upstream 100 bases:

>100_bases
TTGGTGATGGGAAGATTTTTGTTTATGACCTTCAGCAAGCAGTGCGTATTCGTACTGGTGAAACCAATACCGAAGCACTT
TAAGGATAGAGGACAATATT

Downstream 100 bases:

>100_bases
AAAGTTAATATTTCCTTACAAATTCAAGAGCCCTAATGCGCAAGTATTGGGGCTCTTTTTTATCAAAAAGAAAATTGGAT
CATTGTCTTTTTTAAATCTC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MELSTTVSELRYALDTFFFLMSGALVMWMAAGFAMLEAGLVRSKNTTEILTKNICLYAIACTTYLVVGYNIMYVDNGSGG
FLPSIGTLIGTQAEGADHSLESDFFFQVVFVATAMSVVSGAVAERMKLWSFLIFSAILTAFIYPMEGYWTWGGGFLSEAG
FSDFAGSGIVHMAGASAALAGVLLLGARKGKYGKKGQIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMVSDFENATAV
GQIFLNTNAAAAAGAITALFVCKSTWGKADLTMVLNGALAGLVAITADPLSPSPLAAVSVGAVAGALVVFSIVGFDKIKI
DDPVGAISVHGVCGFFGLMVVPLSNSDATFGAQLLGAVVIFAWVFGASLVVWAVLKATVGIRVTEEEELEGMDVHDCGID
AYPEFVSVK

Sequences:

>Translated_409_residues
MELSTTVSELRYALDTFFFLMSGALVMWMAAGFAMLEAGLVRSKNTTEILTKNICLYAIACTTYLVVGYNIMYVDNGSGG
FLPSIGTLIGTQAEGADHSLESDFFFQVVFVATAMSVVSGAVAERMKLWSFLIFSAILTAFIYPMEGYWTWGGGFLSEAG
FSDFAGSGIVHMAGASAALAGVLLLGARKGKYGKKGQIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMVSDFENATAV
GQIFLNTNAAAAAGAITALFVCKSTWGKADLTMVLNGALAGLVAITADPLSPSPLAAVSVGAVAGALVVFSIVGFDKIKI
DDPVGAISVHGVCGFFGLMVVPLSNSDATFGAQLLGAVVIFAWVFGASLVVWAVLKATVGIRVTEEEELEGMDVHDCGID
AYPEFVSVK
>Mature_409_residues
MELSTTVSELRYALDTFFFLMSGALVMWMAAGFAMLEAGLVRSKNTTEILTKNICLYAIACTTYLVVGYNIMYVDNGSGG
FLPSIGTLIGTQAEGADHSLESDFFFQVVFVATAMSVVSGAVAERMKLWSFLIFSAILTAFIYPMEGYWTWGGGFLSEAG
FSDFAGSGIVHMAGASAALAGVLLLGARKGKYGKKGQIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMVSDFENATAV
GQIFLNTNAAAAAGAITALFVCKSTWGKADLTMVLNGALAGLVAITADPLSPSPLAAVSVGAVAGALVVFSIVGFDKIKI
DDPVGAISVHGVCGFFGLMVVPLSNSDATFGAQLLGAVVIFAWVFGASLVVWAVLKATVGIRVTEEEELEGMDVHDCGID
AYPEFVSVK

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=409, Percent_Identity=30.8068459657702, Blast_Score=158, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17550180, Length=423, Percent_Identity=35.2245862884161, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17550182, Length=426, Percent_Identity=32.8638497652582, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI32564042, Length=429, Percent_Identity=27.7389277389277, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI133931100, Length=344, Percent_Identity=29.9418604651163, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6325396, Length=421, Percent_Identity=27.790973871734, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6321559, Length=421, Percent_Identity=27.0783847980998, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324187, Length=412, Percent_Identity=27.6699029126214, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078314, Length=414, Percent_Identity=32.1256038647343, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42869; Mature: 42869

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSTTVSELRYALDTFFFLMSGALVMWMAAGFAMLEAGLVRSKNTTEILTKNICLYAIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
CTTYLVVGYNIMYVDNGSGGFLPSIGTLIGTQAEGADHSLESDFFFQVVFVATAMSVVSG
HHHHHHHCEEEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AVAERMKLWSFLIFSAILTAFIYPMEGYWTWGGGFLSEAGFSDFAGSGIVHMAGASAALA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHCCCCCCCCCCCEEEECCHHHHHH
GVLLLGARKGKYGKKGQIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMVSDFENATAV
HHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEE
GQIFLNTNAAAAAGAITALFVCKSTWGKADLTMVLNGALAGLVAITADPLSPSPLAAVSV
EEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHHHH
GAVAGALVVFSIVGFDKIKIDDPVGAISVHGVCGFFGLMVVPLSNSDATFGAQLLGAVVI
HHHHHHHHHHHHHCCCEEEECCCCCEEEHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHH
FAWVFGASLVVWAVLKATVGIRVTEEEELEGMDVHDCGIDAYPEFVSVK
HHHHHHHHHHHHHHHHHHHCEEECCHHHCCCCCCCCCCCCCCCCCEECC
>Mature Secondary Structure
MELSTTVSELRYALDTFFFLMSGALVMWMAAGFAMLEAGLVRSKNTTEILTKNICLYAIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
CTTYLVVGYNIMYVDNGSGGFLPSIGTLIGTQAEGADHSLESDFFFQVVFVATAMSVVSG
HHHHHHHCEEEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AVAERMKLWSFLIFSAILTAFIYPMEGYWTWGGGFLSEAGFSDFAGSGIVHMAGASAALA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHCCCCCCCCCCCEEEECCHHHHHH
GVLLLGARKGKYGKKGQIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMVSDFENATAV
HHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEE
GQIFLNTNAAAAAGAITALFVCKSTWGKADLTMVLNGALAGLVAITADPLSPSPLAAVSV
EEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHHHH
GAVAGALVVFSIVGFDKIKIDDPVGAISVHGVCGFFGLMVVPLSNSDATFGAQLLGAVVI
HHHHHHHHHHHHHCCCEEEECCCCCEEEHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHH
FAWVFGASLVVWAVLKATVGIRVTEEEELEGMDVHDCGIDAYPEFVSVK
HHHHHHHHHHHHHHHHHHHCEEECCHHHCCCCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]