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The map label for this gene is glnK [H]

Identifier: 59712760

GI number: 59712760

Start: 2410782

End: 2411120

Strand: Reverse

Name: glnK [H]

Synonym: VF_2153

Alternate gene names: 59712760

Gene position: 2411120-2410782 (Counterclockwise)

Preceding gene: 59712761

Following gene: 172087755

Centisome position: 83.21

GC content: 41.89

Gene sequence:

>339_bases
ATGAAAATAATTAACGCCATAATTAAACCATTTAAATTAGACGACGTTAGAGAAGCGCTTGCCGATGCTGGAGTAGATGG
CATGACCGTTTCAGAAGTGAAAGGATTTGGACGTCAAAAAGGTCATACTGAACTATATCGAGGGGCTGAGTATCAAGTCG
ATTTTCTTCCAAAAGTAAAATTAGAAATAGCCGTTCAAGCGGAAGGGGTTGACCGTATTGTTGAAGCGATCAGCAAGGCG
GCGCACACAGGTAAGATTGGTGATGGGAAGATTTTTGTTTATGACCTTCAGCAAGCAGTGCGTATTCGTACTGGTGAAAC
CAATACCGAAGCACTTTAA

Upstream 100 bases:

>100_bases
TCTGCATTTTTCTATCTTATTGAATAATAATTATTTTTAATGTTGGCACACCGATTGAATTGTTAAGAATAAGTAACAAA
TAAATTCAAGGGACTGAGCT

Downstream 100 bases:

>100_bases
GGATAGAGGACAATATTATGGAACTTTCAACAACAGTATCAGAACTTCGTTATGCGTTAGATACCTTCTTCTTTTTGATG
TCAGGTGCATTAGTTATGTG

Product: nitrogen regulatory protein GlnK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKIINAIIKPFKLDDVREALADAGVDGMTVSEVKGFGRQKGHTELYRGAEYQVDFLPKVKLEIAVQAEGVDRIVEAISKA
AHTGKIGDGKIFVYDLQQAVRIRTGETNTEAL

Sequences:

>Translated_112_residues
MKIINAIIKPFKLDDVREALADAGVDGMTVSEVKGFGRQKGHTELYRGAEYQVDFLPKVKLEIAVQAEGVDRIVEAISKA
AHTGKIGDGKIFVYDLQQAVRIRTGETNTEAL
>Mature_112_residues
MKIINAIIKPFKLDDVREALADAGVDGMTVSEVKGFGRQKGHTELYRGAEYQVDFLPKVKLEIAVQAEGVDRIVEAISKA
AHTGKIGDGKIFVYDLQQAVRIRTGETNTEAL

Specific function: P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=70.5357142857143, Blast_Score=162, Evalue=6e-42,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=64.2857142857143, Blast_Score=159, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12273; Mature: 12273

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIINAIIKPFKLDDVREALADAGVDGMTVSEVKGFGRQKGHTELYRGAEYQVDFLPKVK
CCHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHCCCCCEEECCCCEE
LEIAVQAEGVDRIVEAISKAAHTGKIGDGKIFVYDLQQAVRIRTGETNTEAL
EEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIINAIIKPFKLDDVREALADAGVDGMTVSEVKGFGRQKGHTELYRGAEYQVDFLPKVK
CCHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHCCCCCEEECCCCEE
LEIAVQAEGVDRIVEAISKAAHTGKIGDGKIFVYDLQQAVRIRTGETNTEAL
EEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]