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The map label for this gene is tilS [H]

Identifier: 172087739

GI number: 172087739

Start: 2171903

End: 2173273

Strand: Reverse

Name: tilS [H]

Synonym: VF_1945

Alternate gene names: 172087739

Gene position: 2173273-2171903 (Counterclockwise)

Preceding gene: 59712553

Following gene: 59712550

Centisome position: 75.0

GC content: 37.93

Gene sequence:

>1371_bases
GTGCTCGCTTTTTTATTGGAAGAAACCTTAGGTTTAATTGATATGCTTTATTCTCATTTTTCTCAGCAAATAACATCTTA
TTCCGTTAAAAAACCACATCTTATTGTCGCATTAAGTGGTGGCGTTGATTCAATGGTGTTATTGCGTTTAGCTGGAATGT
ACGCGAAGGAAAAGCAATTGGAATGTATGGCTGTTCATGTTAATCATGGATTAAGTCTGCATGCTAATGATTGGGAAGTG
AGTTGCGCTTCTTATTGTAAAGAATTAGATATTCCGCTTTATATCGAGCGAGTAAATTTAAAAGTAAGTCCGCAAGAGAG
CTTAGAGGAAGTGGCAAGAAAAGCTCGTTATCATGCGTTAGAAAAGTATATGCAATCGAATAGTTTATTACTTACGGGTC
AACACCAAGACGATCAAGTAGAAACGTTTTTCTTAGCGCTTAAGCGTGGCAGTGGTCCTGCTGGACTCTCTTCTATGCCT
TCAATTATCCCGCTTAAAACTGGGTATAAATGCCGACCGTTATTATTTACCTCTCGTCAGCACATTACAGAGTATGCTCA
ACAGCATCAGTTAACTTGGGTTGAAGATGAAAGTAATCAAGATACTCGATTTGATCGTAATTTCTTACGTCATGAGATAA
CTCCCGTGTTATCTCAACGTTGGCCAAGTTTTGCTTCTGCTGTTTCTCGAAGTGCTCAATTATGTGCGGAGCAAGAAGCG
TTGTTGGTTGAATTATTGCAACCTAAATTGCAACTGTTTCAAAATGAATTTCAAGGTCTATCTATCAAACAAATTGAACC
AGAATCACTAGCATCTCGTAATATGTTATTGCGACTATGGCTGAAATTGTTTGCTATCCCACTTCCTAGTCAAACTCAAC
TTCATATTTTATGGAATGAGGTTGCTATGGCTCAACAAGATGCAAATCCTTGTTTAGAGTTGGGAATTGTACAAATACGA
CGTTATCAAGGTTATATTTATTGTGTGCCTAAATACCAAGATCTGTCTTTGTGGAAAAAGGCGTTAGAAGGAAAAATTGC
ACTACCAGATAATCTTGGTGAGCTAGAGCTGAGAGCTAAGTTTAGTAATCATGAGAAGAGCAGTATTTTACAATTAAGAG
CACCGTTACCTAATGAAAAAGTAGAGGTGCGTTTTAATGTTGTTGGTTTGGCGCCACATCCCGAGAAACGTCAACATAGC
CGTAAAATGAAAAAACTGTATCAAGAGTATGAAGTACCTACTTGGCAACGTTCACGATTACCAATGGTGTTTTATAATGA
TGAATTAGCTGCTGTTGCTGGATTGTTTGTTTGCAAAGCATTTTCAGGGCAAGAATGTGAGCTACTTTGGCATAAACCTG
TATCAGAATAG

Upstream 100 bases:

>100_bases
CAACGCTTAATGAGCTATGGTTACTGCTGATAGCATTTATTGATTCGCGCACAGTAGTCGCGTAATCCAATAAGATAGAG
TAAAGTGAAAGCGAGCCTGA

Downstream 100 bases:

>100_bases
ACATTCAAAACGAGCTTGGCACAATAACTTAAAATTATAAGGAGCAATGGAATGAAAAAAATAATTGGATTAGCGGTTAT
CGCATTAATGGCCTCAGCAC

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MLAFLLEETLGLIDMLYSHFSQQITSYSVKKPHLIVALSGGVDSMVLLRLAGMYAKEKQLECMAVHVNHGLSLHANDWEV
SCASYCKELDIPLYIERVNLKVSPQESLEEVARKARYHALEKYMQSNSLLLTGQHQDDQVETFFLALKRGSGPAGLSSMP
SIIPLKTGYKCRPLLFTSRQHITEYAQQHQLTWVEDESNQDTRFDRNFLRHEITPVLSQRWPSFASAVSRSAQLCAEQEA
LLVELLQPKLQLFQNEFQGLSIKQIEPESLASRNMLLRLWLKLFAIPLPSQTQLHILWNEVAMAQQDANPCLELGIVQIR
RYQGYIYCVPKYQDLSLWKKALEGKIALPDNLGELELRAKFSNHEKSSILQLRAPLPNEKVEVRFNVVGLAPHPEKRQHS
RKMKKLYQEYEVPTWQRSRLPMVFYNDELAAVAGLFVCKAFSGQECELLWHKPVSE

Sequences:

>Translated_456_residues
MLAFLLEETLGLIDMLYSHFSQQITSYSVKKPHLIVALSGGVDSMVLLRLAGMYAKEKQLECMAVHVNHGLSLHANDWEV
SCASYCKELDIPLYIERVNLKVSPQESLEEVARKARYHALEKYMQSNSLLLTGQHQDDQVETFFLALKRGSGPAGLSSMP
SIIPLKTGYKCRPLLFTSRQHITEYAQQHQLTWVEDESNQDTRFDRNFLRHEITPVLSQRWPSFASAVSRSAQLCAEQEA
LLVELLQPKLQLFQNEFQGLSIKQIEPESLASRNMLLRLWLKLFAIPLPSQTQLHILWNEVAMAQQDANPCLELGIVQIR
RYQGYIYCVPKYQDLSLWKKALEGKIALPDNLGELELRAKFSNHEKSSILQLRAPLPNEKVEVRFNVVGLAPHPEKRQHS
RKMKKLYQEYEVPTWQRSRLPMVFYNDELAAVAGLFVCKAFSGQECELLWHKPVSE
>Mature_456_residues
MLAFLLEETLGLIDMLYSHFSQQITSYSVKKPHLIVALSGGVDSMVLLRLAGMYAKEKQLECMAVHVNHGLSLHANDWEV
SCASYCKELDIPLYIERVNLKVSPQESLEEVARKARYHALEKYMQSNSLLLTGQHQDDQVETFFLALKRGSGPAGLSSMP
SIIPLKTGYKCRPLLFTSRQHITEYAQQHQLTWVEDESNQDTRFDRNFLRHEITPVLSQRWPSFASAVSRSAQLCAEQEA
LLVELLQPKLQLFQNEFQGLSIKQIEPESLASRNMLLRLWLKLFAIPLPSQTQLHILWNEVAMAQQDANPCLELGIVQIR
RYQGYIYCVPKYQDLSLWKKALEGKIALPDNLGELELRAKFSNHEKSSILQLRAPLPNEKVEVRFNVVGLAPHPEKRQHS
RKMKKLYQEYEVPTWQRSRLPMVFYNDELAAVAGLFVCKAFSGQECELLWHKPVSE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=433, Percent_Identity=41.5704387990762, Blast_Score=303, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 52347; Mature: 52347

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAFLLEETLGLIDMLYSHFSQQITSYSVKKPHLIVALSGGVDSMVLLRLAGMYAKEKQL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCC
ECMAVHVNHGLSLHANDWEVSCASYCKELDIPLYIERVNLKVSPQESLEEVARKARYHAL
EEEEEEECCCEEEECCCCCCHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHH
EKYMQSNSLLLTGQHQDDQVETFFLALKRGSGPAGLSSMPSIIPLKTGYKCRPLLFTSRQ
HHHHCCCCEEEECCCCCCHHHHHEEEEECCCCCCCCCCCCCCEEECCCCCCCEEEECCHH
HITEYAQQHQLTWVEDESNQDTRFDRNFLRHEITPVLSQRWPSFASAVSRSAQLCAEQEA
HHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LLVELLQPKLQLFQNEFQGLSIKQIEPESLASRNMLLRLWLKLFAIPLPSQTQLHILWNE
HHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHH
VAMAQQDANPCLELGIVQIRRYQGYIYCVPKYQDLSLWKKALEGKIALPDNLGELELRAK
HHHHHCCCCHHHHHHHHHHEEECCEEEEECCCCCHHHHHHHHCCCEECCCCCCCEEEEEE
FSNHEKSSILQLRAPLPNEKVEVRFNVVGLAPHPEKRQHSRKMKKLYQEYEVPTWQRSRL
CCCCCHHCEEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
PMVFYNDELAAVAGLFVCKAFSGQECELLWHKPVSE
CEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MLAFLLEETLGLIDMLYSHFSQQITSYSVKKPHLIVALSGGVDSMVLLRLAGMYAKEKQL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCC
ECMAVHVNHGLSLHANDWEVSCASYCKELDIPLYIERVNLKVSPQESLEEVARKARYHAL
EEEEEEECCCEEEECCCCCCHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHH
EKYMQSNSLLLTGQHQDDQVETFFLALKRGSGPAGLSSMPSIIPLKTGYKCRPLLFTSRQ
HHHHCCCCEEEECCCCCCHHHHHEEEEECCCCCCCCCCCCCCEEECCCCCCCEEEECCHH
HITEYAQQHQLTWVEDESNQDTRFDRNFLRHEITPVLSQRWPSFASAVSRSAQLCAEQEA
HHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LLVELLQPKLQLFQNEFQGLSIKQIEPESLASRNMLLRLWLKLFAIPLPSQTQLHILWNE
HHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHH
VAMAQQDANPCLELGIVQIRRYQGYIYCVPKYQDLSLWKKALEGKIALPDNLGELELRAK
HHHHHCCCCHHHHHHHHHHEEECCEEEEECCCCCHHHHHHHHCCCEECCCCCCCEEEEEE
FSNHEKSSILQLRAPLPNEKVEVRFNVVGLAPHPEKRQHSRKMKKLYQEYEVPTWQRSRL
CCCCCHHCEEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
PMVFYNDELAAVAGLFVCKAFSGQECELLWHKPVSE
CEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA