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The map label for this gene is wcaJ [H]

Identifier: 172087657

GI number: 172087657

Start: 382763

End: 384175

Strand: Reverse

Name: wcaJ [H]

Synonym: VF_0352

Alternate gene names: 172087657

Gene position: 384175-382763 (Counterclockwise)

Preceding gene: 59710961

Following gene: 59710958

Centisome position: 13.26

GC content: 35.17

Gene sequence:

>1413_bases
ATGAAACAACAAAATATAAAAATGATAAGTGATAGTTATCGTACCCTAACAAAGGCATTTGACTTTATAGCAATTAACTT
ATTACTGACCTCTATCCTATCCTTTCATGGGCTTGAAGAAACCGCTGTCGATCTATCTGCCGGTCTCTTATTTTCTACTA
TTTTTCTACTTATTAGTGAATATTCAGGTCTGTACACCATCAATACAAAAGTGGAGTTAAAGAAATATCTTTTAAGACTA
ACTTTCTCTTTACTCCTTACGGTATCAGTATTTTTTATATTAAAACTTCGCCTTGCAAGTTTAGAGGGCATGCAGATCAG
TAATCTAAACTCTCATATCTATCTTCTTTGGTATGTTTGTGTTTGGGGGGGTCTCTCTCTTTCTCGAACGCTTTGCACCT
TAAGCTACACCTACATTGTCATACCAAGATTAAAGATAAATAAGATCGCGATCATTGGTTTAACCCCAGCAGGACTTTCA
ATTGAACAAGCGTTGATTTCAAAATATAAGAATCAAAAAATTAGTATTGAATTTTATGATGACCGTTCAAGCAGTCGCTT
TAGTTATGTAAATAAAAGTGGTTATGCAGGAAAAGTGTCAGAGCTGATTGAAAAAGTAAAAAATGATGAAATTCAAAGTG
TGTATATTGCACTGCCTATGGTGGCTAAAGATCGTATCAAAGACATCATTCATCAATTATCAAACTCAACGGTTAATGCT
TACATTGTTCCAGATCTCTACACGTATAAATTAAGTGTTTCGCAAATAAAACGCATTGGAAATGTACATACGTTTAGTAT
TTTCAGTTCACCATTCGAAGGGTTGGGAGCTGTCATTAAGCGTATTGAAGATATCGTTATAGGTTCATTAATCACATTAC
TAATCCTACCGGTTCTATGTATTGTCGCTTTAGGCGTGAAATTAAGCTCTCCAGGTCCTATTCTATTTAAACAGGACAGG
TATGGCCTAGGTGGTAAAAAAATTAAAGTATGGAAGTTCCGTTCAATGAAAGTAATGGAAAATTCTGAAGTAGTTACTCA
AGCAACTAAAAATGATCCTAGGGTTACTCGCTTTGGAAGTTTTATCCGCAGAACATCATTAGATGAACTCCCACAGTTTA
TTAATGTATTACAAGGAAGTATGTCTATTGTTGGCCCTCGACCTCATGCTGTTGCACATAATGAGGAGTACCGCGTGTTA
GTCGACAATTACATGGTAAGACATAAAATTAAACCCGGTATTACTGGGCTAGCACAAATCAATGGCTACCGTGGAGAAAC
CGATACATTAGATAAGATGGAAAAGCGTGTAGAGTATGATATTAAATACTTACAAAACTGGTCTTTATCAATGGATCTTA
AGATTATTTTCTTAACTGTTTTCAAAGGATTTGTGAGTGATACGGCCTACTAA

Upstream 100 bases:

>100_bases
TATCAAAATATATGATGTCATTTTTTTGACTAAACGCTAACTTTACAAGTTTATTCATCATTTATAATGGTGATTACTCC
CCCTAATGCCCAGGAGCGAT

Downstream 100 bases:

>100_bases
AACGCCAATATCTTTCAACAAACTTATACTGCTTACTACTTCATTAGTAAGCAGTTCTTGTTATGCTTATTTAACACCAA
AGCCACATATCGGCCCGGCA

Product: UDP-glucose lipid carrier transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKQQNIKMISDSYRTLTKAFDFIAINLLLTSILSFHGLEETAVDLSAGLLFSTIFLLISEYSGLYTINTKVELKKYLLRL
TFSLLLTVSVFFILKLRLASLEGMQISNLNSHIYLLWYVCVWGGLSLSRTLCTLSYTYIVIPRLKINKIAIIGLTPAGLS
IEQALISKYKNQKISIEFYDDRSSSRFSYVNKSGYAGKVSELIEKVKNDEIQSVYIALPMVAKDRIKDIIHQLSNSTVNA
YIVPDLYTYKLSVSQIKRIGNVHTFSIFSSPFEGLGAVIKRIEDIVIGSLITLLILPVLCIVALGVKLSSPGPILFKQDR
YGLGGKKIKVWKFRSMKVMENSEVVTQATKNDPRVTRFGSFIRRTSLDELPQFINVLQGSMSIVGPRPHAVAHNEEYRVL
VDNYMVRHKIKPGITGLAQINGYRGETDTLDKMEKRVEYDIKYLQNWSLSMDLKIIFLTVFKGFVSDTAY

Sequences:

>Translated_470_residues
MKQQNIKMISDSYRTLTKAFDFIAINLLLTSILSFHGLEETAVDLSAGLLFSTIFLLISEYSGLYTINTKVELKKYLLRL
TFSLLLTVSVFFILKLRLASLEGMQISNLNSHIYLLWYVCVWGGLSLSRTLCTLSYTYIVIPRLKINKIAIIGLTPAGLS
IEQALISKYKNQKISIEFYDDRSSSRFSYVNKSGYAGKVSELIEKVKNDEIQSVYIALPMVAKDRIKDIIHQLSNSTVNA
YIVPDLYTYKLSVSQIKRIGNVHTFSIFSSPFEGLGAVIKRIEDIVIGSLITLLILPVLCIVALGVKLSSPGPILFKQDR
YGLGGKKIKVWKFRSMKVMENSEVVTQATKNDPRVTRFGSFIRRTSLDELPQFINVLQGSMSIVGPRPHAVAHNEEYRVL
VDNYMVRHKIKPGITGLAQINGYRGETDTLDKMEKRVEYDIKYLQNWSLSMDLKIIFLTVFKGFVSDTAY
>Mature_470_residues
MKQQNIKMISDSYRTLTKAFDFIAINLLLTSILSFHGLEETAVDLSAGLLFSTIFLLISEYSGLYTINTKVELKKYLLRL
TFSLLLTVSVFFILKLRLASLEGMQISNLNSHIYLLWYVCVWGGLSLSRTLCTLSYTYIVIPRLKINKIAIIGLTPAGLS
IEQALISKYKNQKISIEFYDDRSSSRFSYVNKSGYAGKVSELIEKVKNDEIQSVYIALPMVAKDRIKDIIHQLSNSTVNA
YIVPDLYTYKLSVSQIKRIGNVHTFSIFSSPFEGLGAVIKRIEDIVIGSLITLLILPVLCIVALGVKLSSPGPILFKQDR
YGLGGKKIKVWKFRSMKVMENSEVVTQATKNDPRVTRFGSFIRRTSLDELPQFINVLQGSMSIVGPRPHAVAHNEEYRVL
VDNYMVRHKIKPGITGLAQINGYRGETDTLDKMEKRVEYDIKYLQNWSLSMDLKIIFLTVFKGFVSDTAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=362, Percent_Identity=50.828729281768, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 53205; Mature: 53205

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQQNIKMISDSYRTLTKAFDFIAINLLLTSILSFHGLEETAVDLSAGLLFSTIFLLISE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
YSGLYTINTKVELKKYLLRLTFSLLLTVSVFFILKLRLASLEGMQISNLNSHIYLLWYVC
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHH
VWGGLSLSRTLCTLSYTYIVIPRLKINKIAIIGLTPAGLSIEQALISKYKNQKISIEFYD
HHCCCHHHHHHHHEEEEEEEEECEEECEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEC
DRSSSRFSYVNKSGYAGKVSELIEKVKNDEIQSVYIALPMVAKDRIKDIIHQLSNSTVNA
CCCCCCEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCEEE
YIVPDLYTYKLSVSQIKRIGNVHTFSIFSSPFEGLGAVIKRIEDIVIGSLITLLILPVLC
EECCCEEEEEEEHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVALGVKLSSPGPILFKQDRYGLGGKKIKVWKFRSMKVMENSEVVTQATKNDPRVTRFGS
HHHHCCEECCCCCEEEECCCCCCCCCEEEEEEEECEEEECCCHHHHHHCCCCCCHHHHHH
FIRRTSLDELPQFINVLQGSMSIVGPRPHAVAHNEEYRVLVDNYMVRHKIKPGITGLAQI
HHHHCCHHHHHHHHHHHCCCCEEECCCCCEECCCCCCEEEEHHHHHHHCCCCCCCCHHHC
NGYRGETDTLDKMEKRVEYDIKYLQNWSLSMDLKIIFLTVFKGFVSDTAY
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKQQNIKMISDSYRTLTKAFDFIAINLLLTSILSFHGLEETAVDLSAGLLFSTIFLLISE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
YSGLYTINTKVELKKYLLRLTFSLLLTVSVFFILKLRLASLEGMQISNLNSHIYLLWYVC
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHH
VWGGLSLSRTLCTLSYTYIVIPRLKINKIAIIGLTPAGLSIEQALISKYKNQKISIEFYD
HHCCCHHHHHHHHEEEEEEEEECEEECEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEC
DRSSSRFSYVNKSGYAGKVSELIEKVKNDEIQSVYIALPMVAKDRIKDIIHQLSNSTVNA
CCCCCCEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCEEE
YIVPDLYTYKLSVSQIKRIGNVHTFSIFSSPFEGLGAVIKRIEDIVIGSLITLLILPVLC
EECCCEEEEEEEHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVALGVKLSSPGPILFKQDRYGLGGKKIKVWKFRSMKVMENSEVVTQATKNDPRVTRFGS
HHHHCCEECCCCCEEEECCCCCCCCCEEEEEEEECEEEECCCHHHHHHCCCCCCHHHHHH
FIRRTSLDELPQFINVLQGSMSIVGPRPHAVAHNEEYRVLVDNYMVRHKIKPGITGLAQI
HHHHCCHHHHHHHHHHHCCCCEEECCCCCEECCCCCCEEEEHHHHHHHCCCCCCCCHHHC
NGYRGETDTLDKMEKRVEYDIKYLQNWSLSMDLKIIFLTVFKGFVSDTAY
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]