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Identifier: 172087639

GI number: 172087639

Start: 107132

End: 108133

Strand: Reverse

Name: add

Synonym: VF_0093

Alternate gene names: 172087639

Gene position: 108133-107132 (Counterclockwise)

Preceding gene: 59710701

Following gene: 172087637

Centisome position: 3.73

GC content: 41.02

Gene sequence:

>1002_bases
ATGATCATTAAACAACTTCCTTTAACGGATCTACACCGTCACCTTGATGGAAATATTCGCATTGAAACCATTTTGGATTT
AGGCCAAAAATTTGGTTTAGACCTACCTGCGTATGATATCGAAGCACTTCGCCCTCACGTACAAATTGTGGAAGCCGAGC
CAAGTCTTGTGGCTTTCTTATCGAAACTTGATTGGGGCGTTGCTGTTCTTGGTGATTTAGATGCCTGTCGTCGTGTGGCA
TATGAAAACGTTCAAGACGCAATGAATGCTCAAATTGATTACGCTGAATTACGCTTTTCACCATACTACATGGCAATGAA
ACACAATTTACCTATTGCTGGTGTTGTTGAAGCGGTTGTTGATGGTGTAGAAGCGGGTTGTCGTGATTTTGGTATTAAGG
CTAACTTAATCGGTATTATGAGTCGTACCTTTGGTCAAGATGCTTGCCAACAAGAATTAGATGGCCTACTGACTCAAAAA
CACAAATTAGTTGCAATCGATCTTGCTGGTGATGAGTTAGGTCAACCAGGTGACCTTTTTGTAAATCACTTTAAACAAGT
GAAAGACGCAGATCTTCGTGTCACTGTACACGCAGGTGAAGCAGCGGGTGCTGCAAGTATGTGGCAAGCAATTAATGAGC
TTGGCGCAGTACGAATTGGTCATGGTGTTAAAGCCATTGAAGATCCAAAACTGATGGAATACCTAGCTAAAAACAACATT
GGTATTGAGTCTTGCTTAACCTCTAACATTCAAACAAGTACGGTTGCTTCATTTGAATCTCACCCAATTAAGACATTCTT
AGATTACGGTGTTAAAGTTTGTCTTAATACAGATGACCCAGCGGTTGAAGGCATTGAATTACCTCATGAGTACGAAGTCG
CTGCACCAAAAGTTGGCTTAACGCCAGAGCAACTTAAACAAATTCAAATTAATGGTTTAGATCTGGCTTTCTTATCTGAT
TCAGAGAAACAAGCTCTACGTGAGATGGCAGCTAAGCGCTAA

Upstream 100 bases:

>100_bases
CACACTTGTCTCTATACTCTGCTGTAATATCTACTTATATTCAGTTTTATACCAATGCCGATGCACCAATGGAATTGGGA
CACTTTTTAGGAAAATAATA

Downstream 100 bases:

>100_bases
ATAAAGCAGGTTTCAGGGCGCTGCGCTTACAGGATTCAGGGGTACTACTTCTGCTCTTCCTGAAGCCTGAACCCTGAAAC
CTATCTTTTACTCTTATCGT

Product: adenosine deaminase

Products: NA

Alternate protein names: Adenosine aminohydrolase

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MIIKQLPLTDLHRHLDGNIRIETILDLGQKFGLDLPAYDIEALRPHVQIVEAEPSLVAFLSKLDWGVAVLGDLDACRRVA
YENVQDAMNAQIDYAELRFSPYYMAMKHNLPIAGVVEAVVDGVEAGCRDFGIKANLIGIMSRTFGQDACQQELDGLLTQK
HKLVAIDLAGDELGQPGDLFVNHFKQVKDADLRVTVHAGEAAGAASMWQAINELGAVRIGHGVKAIEDPKLMEYLAKNNI
GIESCLTSNIQTSTVASFESHPIKTFLDYGVKVCLNTDDPAVEGIELPHEYEVAAPKVGLTPEQLKQIQINGLDLAFLSD
SEKQALREMAAKR

Sequences:

>Translated_333_residues
MIIKQLPLTDLHRHLDGNIRIETILDLGQKFGLDLPAYDIEALRPHVQIVEAEPSLVAFLSKLDWGVAVLGDLDACRRVA
YENVQDAMNAQIDYAELRFSPYYMAMKHNLPIAGVVEAVVDGVEAGCRDFGIKANLIGIMSRTFGQDACQQELDGLLTQK
HKLVAIDLAGDELGQPGDLFVNHFKQVKDADLRVTVHAGEAAGAASMWQAINELGAVRIGHGVKAIEDPKLMEYLAKNNI
GIESCLTSNIQTSTVASFESHPIKTFLDYGVKVCLNTDDPAVEGIELPHEYEVAAPKVGLTPEQLKQIQINGLDLAFLSD
SEKQALREMAAKR
>Mature_333_residues
MIIKQLPLTDLHRHLDGNIRIETILDLGQKFGLDLPAYDIEALRPHVQIVEAEPSLVAFLSKLDWGVAVLGDLDACRRVA
YENVQDAMNAQIDYAELRFSPYYMAMKHNLPIAGVVEAVVDGVEAGCRDFGIKANLIGIMSRTFGQDACQQELDGLLTQK
HKLVAIDLAGDELGQPGDLFVNHFKQVKDADLRVTVHAGEAAGAASMWQAINELGAVRIGHGVKAIEDPKLMEYLAKNNI
GIESCLTSNIQTSTVASFESHPIKTFLDYGVKVCLNTDDPAVEGIELPHEYEVAAPKVGLTPEQLKQIQINGLDLAFLSD
SEKQALREMAAKR

Specific function: Unknown

COG id: COG1816

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenosine and AMP deaminases family

Homologues:

Organism=Homo sapiens, GI47078295, Length=343, Percent_Identity=32.6530612244898, Blast_Score=150, Evalue=1e-36,
Organism=Escherichia coli, GI1787910, Length=333, Percent_Identity=63.6636636636637, Blast_Score=461, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI32565969, Length=337, Percent_Identity=32.9376854599407, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17538512, Length=337, Percent_Identity=32.9376854599407, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI115537133, Length=322, Percent_Identity=25.1552795031056, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324188, Length=292, Percent_Identity=24.6575342465753, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24645260, Length=328, Percent_Identity=25, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45553183, Length=185, Percent_Identity=28.6486486486486, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17737615, Length=185, Percent_Identity=28.6486486486486, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ADD_VIBF1 (Q5E8Q8)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203476.2
- HSSP:   Q7RMV2
- ProteinModelPortal:   Q5E8Q8
- SMR:   Q5E8Q8
- STRING:   Q5E8Q8
- GeneID:   3277897
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0093
- NMPDR:   fig|312309.3.peg.93
- eggNOG:   COG1816
- HOGENOM:   HBG630382
- OMA:   LAGNENI
- ProtClustDB:   PRK09358
- BioCyc:   VFIS312309:VF0093-MONOMER
- HAMAP:   MF_00540
- InterPro:   IPR001365
- InterPro:   IPR006330
- TIGRFAMs:   TIGR01430

Pfam domain/function: PF00962 A_deaminase

EC number: =3.5.4.4

Molecular weight: Translated: 36466; Mature: 36466

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00485 A_DEAMINASE

Important sites: ACT_SITE 200-200 BINDING 14-14 BINDING 16-16 BINDING 170-170 BINDING 279-279

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKQLPLTDLHRHLDGNIRIETILDLGQKFGLDLPAYDIEALRPHVQIVEAEPSLVAFL
CCCCCCCHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCHHHHCCCEEEEECCHHHHHHH
SKLDWGVAVLGDLDACRRVAYENVQDAMNAQIDYAELRFSPYYMAMKHNLPIAGVVEAVV
HHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCCHHHEECCCEEEEEECCCCHHHHHHHHH
DGVEAGCRDFGIKANLIGIMSRTFGQDACQQELDGLLTQKHKLVAIDLAGDELGQPGDLF
HHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHH
VNHFKQVKDADLRVTVHAGEAAGAASMWQAINELGAVRIGHGVKAIEDPKLMEYLAKNNI
HHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHCCC
GIESCLTSNIQTSTVASFESHPIKTFLDYGVKVCLNTDDPAVEGIELPHEYEVAAPKVGL
CHHHHHCCCCCHHHHHHCCCCCHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
TPEQLKQIQINGLDLAFLSDSEKQALREMAAKR
CHHHHHEEEECCEEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure
MIIKQLPLTDLHRHLDGNIRIETILDLGQKFGLDLPAYDIEALRPHVQIVEAEPSLVAFL
CCCCCCCHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCHHHHCCCEEEEECCHHHHHHH
SKLDWGVAVLGDLDACRRVAYENVQDAMNAQIDYAELRFSPYYMAMKHNLPIAGVVEAVV
HHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCCHHHEECCCEEEEEECCCCHHHHHHHHH
DGVEAGCRDFGIKANLIGIMSRTFGQDACQQELDGLLTQKHKLVAIDLAGDELGQPGDLF
HHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHH
VNHFKQVKDADLRVTVHAGEAAGAASMWQAINELGAVRIGHGVKAIEDPKLMEYLAKNNI
HHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHCCC
GIESCLTSNIQTSTVASFESHPIKTFLDYGVKVCLNTDDPAVEGIELPHEYEVAAPKVGL
CHHHHHCCCCCHHHHHHCCCCCHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
TPEQLKQIQINGLDLAFLSDSEKQALREMAAKR
CHHHHHEEEECCEEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA