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The map label for this gene is fadA [H]

Identifier: 172087631

GI number: 172087631

Start: 25396

End: 26625

Strand: Reverse

Name: fadA [H]

Synonym: VF_0024

Alternate gene names: 172087631

Gene position: 26625-25396 (Counterclockwise)

Preceding gene: 59710632

Following gene: 59710630

Centisome position: 0.92

GC content: 43.66

Gene sequence:

>1230_bases
ATGATGAATGTTATTACCCAGCGCCTCAATCATTATCAGCACCGTCAGCCTAGGGAAGGATTAATCATGAAAAACGTAGT
TATTGTTGACTGTATTCGAACACCTATGGGTCGCTCAAAAAACGGTGTATTTCGTCATACTCGCGCTGAAGATTTATCAG
CGCACCTAATGAAAGGTTTGCTAAAACGAAATCCAAGTGTCGATCCTAATGACATTGAAGATATCTATTGGGGATGTGTA
CAACAAACGTTAGAACAAGGCTTCAATATTGCACGTAATTCCGCACTACTTGCCGGTTTACCACAAAGTATTGCCGCAAC
AACTGTTAACCGTTTATGTGGCTCATCCATGCAAGCTCTGCATGATGCATCACGTGCCATTATGGTTGGCGATGCCGAAA
TTTGCATAATTGGTGGTGTTGAACACATGGGGCATGTACCAATGAACCATGGCGTTGATTTCCATTCAGGACTTTCAAAA
AGTGTTGCTAAAGCATCAGGAATGATGGGATTAACAGCTGAAATGCTAGGTAAAATGCACGGTATATCTCGAGAGCAGCA
AGATGCCTTTGCGCTCGCATCCCATCAAAAAGCACATAAAGCAACGATTGAAGGCTATTTTGATTCGGAGATCCTTCCAA
TGGAAGGTCACGATGAAAATGGCGCTTTAACCTTAGTCACACATGATGAGGTCATTCGACCTGAAACAACATTAGAAGGA
TTAGCCGCATTACGACCAGCTTTCGATCCTGCAAATGGTACAGTAACAGCAGGCAGTTCATCGGCATTATCGGATGGCGC
ATCAGCGATGCTAGTTATGAGCGAAGAGAAAGCCAATGAGTTAGGTTTACCTATTCGAGCGAAAGTTCGCTCAATGGCGG
TATCTGGTTGCGATCCATCTATTATGGGATACGGCCCTGTTCCAGCGACGAAAAAAGCATTAAAACGTGCCGGATTATCA
CTGGATGATATAGAGCTATTCGAACTGAATGAAGCGTTTGCCGCTCAATCACTACCTTGTATTAAAGATTTAGGCTTACT
TGATGTGATGGATGAAAAGGTCAACTTAAATGGTGGTGCTATCGCTCTTGGTCATCCTCTGGGTTGCTCTGGATCTCGCA
TTGCCACCACATTAATCAATAATATGGAGCGAACTGGAGCCAAGTTAGGTGTTGCAACAATGTGTATTGGATTAGGGCAA
GGGATTGCAACTGTGTTTGAGCGCCCTTAA

Upstream 100 bases:

>100_bases
GATGCAATTGGATTAACCACTTATCTCGACATGGCAAAAGAATTTGAACATCTAGGTGCCGTTTATCAAGTACCAGAAAG
CATCAAACAAAAAGCAGCCG

Downstream 100 bases:

>100_bases
TACTAAGTAAATTCAAACGTTAAAAACAAAAAAAGTCGCTCTCTGTTCTATGAACATTGAAGCGACTTTTTCTTTCTATC
TATAGCTGAGTGCTATAAAC

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MMNVITQRLNHYQHRQPREGLIMKNVVIVDCIRTPMGRSKNGVFRHTRAEDLSAHLMKGLLKRNPSVDPNDIEDIYWGCV
QQTLEQGFNIARNSALLAGLPQSIAATTVNRLCGSSMQALHDASRAIMVGDAEICIIGGVEHMGHVPMNHGVDFHSGLSK
SVAKASGMMGLTAEMLGKMHGISREQQDAFALASHQKAHKATIEGYFDSEILPMEGHDENGALTLVTHDEVIRPETTLEG
LAALRPAFDPANGTVTAGSSSALSDGASAMLVMSEEKANELGLPIRAKVRSMAVSGCDPSIMGYGPVPATKKALKRAGLS
LDDIELFELNEAFAAQSLPCIKDLGLLDVMDEKVNLNGGAIALGHPLGCSGSRIATTLINNMERTGAKLGVATMCIGLGQ
GIATVFERP

Sequences:

>Translated_409_residues
MMNVITQRLNHYQHRQPREGLIMKNVVIVDCIRTPMGRSKNGVFRHTRAEDLSAHLMKGLLKRNPSVDPNDIEDIYWGCV
QQTLEQGFNIARNSALLAGLPQSIAATTVNRLCGSSMQALHDASRAIMVGDAEICIIGGVEHMGHVPMNHGVDFHSGLSK
SVAKASGMMGLTAEMLGKMHGISREQQDAFALASHQKAHKATIEGYFDSEILPMEGHDENGALTLVTHDEVIRPETTLEG
LAALRPAFDPANGTVTAGSSSALSDGASAMLVMSEEKANELGLPIRAKVRSMAVSGCDPSIMGYGPVPATKKALKRAGLS
LDDIELFELNEAFAAQSLPCIKDLGLLDVMDEKVNLNGGAIALGHPLGCSGSRIATTLINNMERTGAKLGVATMCIGLGQ
GIATVFERP
>Mature_409_residues
MMNVITQRLNHYQHRQPREGLIMKNVVIVDCIRTPMGRSKNGVFRHTRAEDLSAHLMKGLLKRNPSVDPNDIEDIYWGCV
QQTLEQGFNIARNSALLAGLPQSIAATTVNRLCGSSMQALHDASRAIMVGDAEICIIGGVEHMGHVPMNHGVDFHSGLSK
SVAKASGMMGLTAEMLGKMHGISREQQDAFALASHQKAHKATIEGYFDSEILPMEGHDENGALTLVTHDEVIRPETTLEG
LAALRPAFDPANGTVTAGSSSALSDGASAMLVMSEEKANELGLPIRAKVRSMAVSGCDPSIMGYGPVPATKKALKRAGLS
LDDIELFELNEAFAAQSLPCIKDLGLLDVMDEKVNLNGGAIALGHPLGCSGSRIATTLINNMERTGAKLGVATMCIGLGQ
GIATVFERP

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=394, Percent_Identity=43.6548223350254, Blast_Score=281, Evalue=8e-76,
Organism=Homo sapiens, GI167614485, Length=399, Percent_Identity=40.3508771929825, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI148539872, Length=396, Percent_Identity=39.8989898989899, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI4504327, Length=431, Percent_Identity=35.2668213457077, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI4557237, Length=409, Percent_Identity=32.0293398533007, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI194353979, Length=241, Percent_Identity=35.2697095435685, Blast_Score=118, Evalue=8e-27,
Organism=Escherichia coli, GI48994986, Length=386, Percent_Identity=73.8341968911917, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=45.049504950495, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=42.8927680798005, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1788554, Length=400, Percent_Identity=43.5, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI1788683, Length=413, Percent_Identity=33.6561743341404, Blast_Score=185, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI133906874, Length=398, Percent_Identity=38.6934673366834, Blast_Score=249, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17535921, Length=399, Percent_Identity=35.3383458646617, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI25147385, Length=398, Percent_Identity=33.1658291457286, Blast_Score=181, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17551802, Length=434, Percent_Identity=32.7188940092166, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17535917, Length=403, Percent_Identity=27.7915632754342, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6322031, Length=409, Percent_Identity=37.4083129584352, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6325229, Length=402, Percent_Identity=36.5671641791045, Blast_Score=218, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24655093, Length=402, Percent_Identity=41.044776119403, Blast_Score=278, Evalue=4e-75,
Organism=Drosophila melanogaster, GI17648125, Length=334, Percent_Identity=42.814371257485, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI17137578, Length=432, Percent_Identity=34.2592592592593, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24640423, Length=418, Percent_Identity=30.8612440191388, Blast_Score=190, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 43597; Mature: 43597

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNVITQRLNHYQHRQPREGLIMKNVVIVDCIRTPMGRSKNGVFRHTRAEDLSAHLMKGL
CHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHHHH
LKRNPSVDPNDIEDIYWGCVQQTLEQGFNIARNSALLAGLPQSIAATTVNRLCGSSMQAL
HHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCHHHHHHHHHHHHCHHHHHH
HDASRAIMVGDAEICIIGGVEHMGHVPMNHGVDFHSGLSKSVAKASGMMGLTAEMLGKMH
HHCCCEEEECCCCEEEEECHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
GISREQQDAFALASHQKAHKATIEGYFDSEILPMEGHDENGALTLVTHDEVIRPETTLEG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHH
LAALRPAFDPANGTVTAGSSSALSDGASAMLVMSEEKANELGLPIRAKVRSMAVSGCDPS
HHHHCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCHHHCCCCHHHHHHHHHHCCCCCC
IMGYGPVPATKKALKRAGLSLDDIELFELNEAFAAQSLPCIKDLGLLDVMDEKVNLNGGA
CCCCCCCCHHHHHHHHCCCCCCCCCEEECCHHHHHCCCCHHHHCCHHHHHHHHCCCCCCE
IALGHPLGCSGSRIATTLINNMERTGAKLGVATMCIGLGQGIATVFERP
EEECCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MMNVITQRLNHYQHRQPREGLIMKNVVIVDCIRTPMGRSKNGVFRHTRAEDLSAHLMKGL
CHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHHHH
LKRNPSVDPNDIEDIYWGCVQQTLEQGFNIARNSALLAGLPQSIAATTVNRLCGSSMQAL
HHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCHHHHHHHHHHHHCHHHHHH
HDASRAIMVGDAEICIIGGVEHMGHVPMNHGVDFHSGLSKSVAKASGMMGLTAEMLGKMH
HHCCCEEEECCCCEEEEECHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
GISREQQDAFALASHQKAHKATIEGYFDSEILPMEGHDENGALTLVTHDEVIRPETTLEG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHH
LAALRPAFDPANGTVTAGSSSALSDGASAMLVMSEEKANELGLPIRAKVRSMAVSGCDPS
HHHHCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCHHHCCCCHHHHHHHHHHCCCCCC
IMGYGPVPATKKALKRAGLSLDDIELFELNEAFAAQSLPCIKDLGLLDVMDEKVNLNGGA
CCCCCCCCHHHHHHHHCCCCCCCCCEEECCHHHHHCCCCHHHHCCHHHHHHHHCCCCCCE
IALGHPLGCSGSRIATTLINNMERTGAKLGVATMCIGLGQGIATVFERP
EEECCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA