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The map label for this gene is hemK [H]

Identifier: 58617581

GI number: 58617581

Start: 1366431

End: 1367273

Strand: Reverse

Name: hemK [H]

Synonym: ERGA_CDS_08540

Alternate gene names: 58617581

Gene position: 1367273-1366431 (Counterclockwise)

Preceding gene: 58617582

Following gene: 58617579

Centisome position: 91.16

GC content: 29.3

Gene sequence:

>843_bases
ATGAATACTATAGCCTCTTTATTTAATAATGCTGTTGATTTTTTAAATAACCATAATATAGACAATCCAAAGCGTGATGT
AGAAGTCATTATAAGACATTTGATGAATGTTGATGGCTTAATTGTAGTAAAAGATCCATATATTTCTCTAAATAAAACTG
ATACAGAGATATTTTGGGATATGGTAAAAAAACGTGCTTTAAATGTACCTATATCACATATTATAGGTAAACGAGAGTTT
TGGAGTATGGATTTTATTGTAAATTCTCATGTATTAGATCCAAGGCCAGATAGTGAAAGTATAATTTCTGCTGTATTTAC
AATGTATCCTTGTAAAAATCGTAGATTAGTAATAGGAGATTTTGGAACAGGCACTGGATGTTTACTTACGGTTTTACTTT
CTTATTATAAGTTAGCAGTTGGTATAGCTGTAGAAAAAAATGTTAAGGCTTATAGGGTAGCATATCAAAATTTTAAAAAT
CATAAGGTATACAATAGAATTAAGATGAGGTTATCTTCTTGGAATACTTGTTACGAAATGTGTGATCTAATAGTTAGTAA
TCCTCCTTATATTAGAAGAAATAAGATTATGACATTACAACCAGAAGTAAAGCTGTATGAGCCATATGTTGCTTTAGATG
GTGGTCCAGTAGGATTGGAAAATTATTTTAGTATTTTTGTGGTGATAAAGAGATGTTTAAAAAAAAATGGTGTTGCTGTT
CTAGAAATAGGAGAAGATCAGCTCCAGATTCATCACATAGTTTGTAAATATGGGTTAAAATTTCATTCATACTGTTATGA
TTTATCTGGCAAAAAACGTTGTATAATAATTAAACAGGTCTGA

Upstream 100 bases:

>100_bases
TCAGTTCGAACATTCTTTAGGTGTAACTGAGAATGGTGTAGAAATATTTACCTTATCTCCAAAGAATTTGCATTATCCAC
CTTATGTATGTTGATTTATA

Downstream 100 bases:

>100_bases
AATAACTAATATTATTGAAGTTTTAAACTTCCTTATTTTGTATAATAAAATATCTGTAAAAGTTTTATATAATCAACAGT
GCATTTTTATTTTTAATGTC

Product: bifunctional methyltransferase HemK/tRNA (guanine-N(7)-)-methyltransferase protoporphyrinogen oxidase

Products: NA

Alternate protein names: HemK protein homolog; M.RcoHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MNTIASLFNNAVDFLNNHNIDNPKRDVEVIIRHLMNVDGLIVVKDPYISLNKTDTEIFWDMVKKRALNVPISHIIGKREF
WSMDFIVNSHVLDPRPDSESIISAVFTMYPCKNRRLVIGDFGTGTGCLLTVLLSYYKLAVGIAVEKNVKAYRVAYQNFKN
HKVYNRIKMRLSSWNTCYEMCDLIVSNPPYIRRNKIMTLQPEVKLYEPYVALDGGPVGLENYFSIFVVIKRCLKKNGVAV
LEIGEDQLQIHHIVCKYGLKFHSYCYDLSGKKRCIIIKQV

Sequences:

>Translated_280_residues
MNTIASLFNNAVDFLNNHNIDNPKRDVEVIIRHLMNVDGLIVVKDPYISLNKTDTEIFWDMVKKRALNVPISHIIGKREF
WSMDFIVNSHVLDPRPDSESIISAVFTMYPCKNRRLVIGDFGTGTGCLLTVLLSYYKLAVGIAVEKNVKAYRVAYQNFKN
HKVYNRIKMRLSSWNTCYEMCDLIVSNPPYIRRNKIMTLQPEVKLYEPYVALDGGPVGLENYFSIFVVIKRCLKKNGVAV
LEIGEDQLQIHHIVCKYGLKFHSYCYDLSGKKRCIIIKQV
>Mature_280_residues
MNTIASLFNNAVDFLNNHNIDNPKRDVEVIIRHLMNVDGLIVVKDPYISLNKTDTEIFWDMVKKRALNVPISHIIGKREF
WSMDFIVNSHVLDPRPDSESIISAVFTMYPCKNRRLVIGDFGTGTGCLLTVLLSYYKLAVGIAVEKNVKAYRVAYQNFKN
HKVYNRIKMRLSSWNTCYEMCDLIVSNPPYIRRNKIMTLQPEVKLYEPYVALDGGPVGLENYFSIFVVIKRCLKKNGVAV
LEIGEDQLQIHHIVCKYGLKFHSYCYDLSGKKRCIIIKQV

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=258, Percent_Identity=23.6434108527132, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1787463, Length=229, Percent_Identity=28.3842794759825, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87082085, Length=214, Percent_Identity=26.6355140186916, Blast_Score=65, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24582226, Length=281, Percent_Identity=27.4021352313167, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 32274; Mature: 32274

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIASLFNNAVDFLNNHNIDNPKRDVEVIIRHLMNVDGLIVVKDPYISLNKTDTEIFWD
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCEECCCCHHHHHHH
MVKKRALNVPISHIIGKREFWSMDFIVNSHVLDPRPDSESIISAVFTMYPCKNRRLVIGD
HHHHHHCCCCHHHHHCCHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEE
FGTGTGCLLTVLLSYYKLAVGIAVEKNVKAYRVAYQNFKNHKVYNRIKMRLSSWNTCYEM
CCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHH
CDLIVSNPPYIRRNKIMTLQPEVKLYEPYVALDGGPVGLENYFSIFVVIKRCLKKNGVAV
HHHHHCCCCCEECCEEEEECCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEE
LEIGEDQLQIHHIVCKYGLKFHSYCYDLSGKKRCIIIKQV
EEECCCHHHHHHHHHHHCCHHHHHEECCCCCCEEEEEECC
>Mature Secondary Structure
MNTIASLFNNAVDFLNNHNIDNPKRDVEVIIRHLMNVDGLIVVKDPYISLNKTDTEIFWD
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCEECCCCHHHHHHH
MVKKRALNVPISHIIGKREFWSMDFIVNSHVLDPRPDSESIISAVFTMYPCKNRRLVIGD
HHHHHHCCCCHHHHHCCHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEE
FGTGTGCLLTVLLSYYKLAVGIAVEKNVKAYRVAYQNFKNHKVYNRIKMRLSSWNTCYEM
CCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHH
CDLIVSNPPYIRRNKIMTLQPEVKLYEPYVALDGGPVGLENYFSIFVVIKRCLKKNGVAV
HHHHHCCCCCEECCEEEEECCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEE
LEIGEDQLQIHHIVCKYGLKFHSYCYDLSGKKRCIIIKQV
EEECCCHHHHHHHHHHHCCHHHHHEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]