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The map label for this gene is lipB

Identifier: 58617385

GI number: 58617385

Start: 1037283

End: 1037909

Strand: Reverse

Name: lipB

Synonym: ERGA_CDS_06580

Alternate gene names: 58617385

Gene position: 1037909-1037283 (Counterclockwise)

Preceding gene: 58617386

Following gene: 58617382

Centisome position: 69.2

GC content: 31.1

Gene sequence:

>627_bases
ATGGAGTGGAAGATTGAATCCTTACCTGTACCTTATGATAAAGCTATGTGTTTTATGCAACAAAGGGTAGAGGGTATTGC
TAATAAGACACAAGATGAACTAGTATGGTTACTTGAACATTTTCCGTTATATACGGCTGGTACTAGTGCAAGGAGTGAGG
AATTACTAACCGATAGTTTATTTCCTGTATATTCTACAGGTAGAGGTGGTAAATACACTTATCATGGTCCTGGTCAAAGA
ATTGCTTATGTGATGATGGATTTAAAAGCAAGAGATAAATGTAATGTTAGGTTGTATGTTGAAACTTTGGGTGAGTGGAT
TGTTAAAACTTTAAAACATTTTTCAATACGATCATATTTTAATCCTAATTTAATAGGGGTATGGGTTAACCATAATGGTA
GTGAAAAAAAAATTGCTGCATTTGGCATTAGAATAAGAAAATGGATAACATATCATGGAGTTTCTATAAATGTTTTTACA
GATCTTTCTCATTATTCAGGAATAATTCCATGTGGTATCAAAGAATATGGAATAACTTCACTTAAAACATTAGGAGTAAA
TATTCTTTATGAAGAATTTGATGTTGTGTTGAAGAAAGAATTTAATAAGGTTTTCTGTAATTGTTAA

Upstream 100 bases:

>100_bases
CTTATCATGTTTGTATTATCAAGTGGTGATAGTTTTATATTTTAATAAATTTTGTATTGGTTTATTATAACGCTATAACA
TGAATGTTATTTGAAGTTAT

Downstream 100 bases:

>100_bases
TCTAATTTTATTATTAAATTACAATCTTATATTAGGATTGAAACGTGTGACAAGACTGGTACAGGTATTTTTAAGTATTT
GTACTATTTGTGATAAGTGA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MEWKIESLPVPYDKAMCFMQQRVEGIANKTQDELVWLLEHFPLYTAGTSARSEELLTDSLFPVYSTGRGGKYTYHGPGQR
IAYVMMDLKARDKCNVRLYVETLGEWIVKTLKHFSIRSYFNPNLIGVWVNHNGSEKKIAAFGIRIRKWITYHGVSINVFT
DLSHYSGIIPCGIKEYGITSLKTLGVNILYEEFDVVLKKEFNKVFCNC

Sequences:

>Translated_208_residues
MEWKIESLPVPYDKAMCFMQQRVEGIANKTQDELVWLLEHFPLYTAGTSARSEELLTDSLFPVYSTGRGGKYTYHGPGQR
IAYVMMDLKARDKCNVRLYVETLGEWIVKTLKHFSIRSYFNPNLIGVWVNHNGSEKKIAAFGIRIRKWITYHGVSINVFT
DLSHYSGIIPCGIKEYGITSLKTLGVNILYEEFDVVLKKEFNKVFCNC
>Mature_208_residues
MEWKIESLPVPYDKAMCFMQQRVEGIANKTQDELVWLLEHFPLYTAGTSARSEELLTDSLFPVYSTGRGGKYTYHGPGQR
IAYVMMDLKARDKCNVRLYVETLGEWIVKTLKHFSIRSYFNPNLIGVWVNHNGSEKKIAAFGIRIRKWITYHGVSINVFT
DLSHYSGIIPCGIKEYGITSLKTLGVNILYEEFDVVLKKEFNKVFCNC

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=184, Percent_Identity=30.4347826086957, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI87081767, Length=162, Percent_Identity=38.8888888888889, Blast_Score=124, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323268, Length=139, Percent_Identity=35.9712230215827, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355483, Length=175, Percent_Identity=37.1428571428571, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_EHRRG (Q5FFZ7)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196584.1
- ProteinModelPortal:   Q5FFZ7
- SMR:   Q5FFZ7
- STRING:   Q5FFZ7
- GeneID:   3268612
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_06580
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- ProtClustDB:   PRK14341
- BioCyc:   ERUM302409:ERGA_CDS_06580-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 23950; Mature: 23950

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWKIESLPVPYDKAMCFMQQRVEGIANKTQDELVWLLEHFPLYTAGTSARSEELLTDSL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCCCHHHHHHHCC
FPVYSTGRGGKYTYHGPGQRIAYVMMDLKARDKCNVRLYVETLGEWIVKTLKHFSIRSYF
CCEEECCCCCCEEEECCCCEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCC
NPNLIGVWVNHNGSEKKIAAFGIRIRKWITYHGVSINVFTDLSHYSGIIPCGIKEYGITS
CCCEEEEEEECCCCCCEEHHHHHEEEEEEEECCEEEEEEECHHHHCCCCCCCCHHHCCHH
LKTLGVNILYEEFDVVLKKEFNKVFCNC
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEWKIESLPVPYDKAMCFMQQRVEGIANKTQDELVWLLEHFPLYTAGTSARSEELLTDSL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCCCHHHHHHHCC
FPVYSTGRGGKYTYHGPGQRIAYVMMDLKARDKCNVRLYVETLGEWIVKTLKHFSIRSYF
CCEEECCCCCCEEEECCCCEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCC
NPNLIGVWVNHNGSEKKIAAFGIRIRKWITYHGVSINVFTDLSHYSGIIPCGIKEYGITS
CCCEEEEEEECCCCCCEEHHHHHEEEEEEEECCEEEEEEECHHHHCCCCCCCCHHHCCHH
LKTLGVNILYEEFDVVLKKEFNKVFCNC
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA