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The map label for this gene is purN [H]

Identifier: 58617386

GI number: 58617386

Start: 1038330

End: 1038968

Strand: Reverse

Name: purN [H]

Synonym: ERGA_CDS_06590

Alternate gene names: 58617386

Gene position: 1038968-1038330 (Counterclockwise)

Preceding gene: 58617387

Following gene: 58617385

Centisome position: 69.27

GC content: 33.96

Gene sequence:

>639_bases
ATGACTATGAAACCACTTAGGTTAGGTATTTTAATTTCAGGTAGGGGTTCTAATATGCAGGCTCTGATTAATGCTTGTCA
GCGAGATGATTTTCCTGCAAGTGTATCCTGTGTTATATCAAATAAATCAAATGCAAACGGTCTAATACTTGCTCAGCAAA
GTAATATTAAAACTTTTATAGTACAAGGTCGTCCTCTAGATTTTGATGCTATTGATAATATACTTGAAGAACATGAGGTG
GATTTAATCTGTCTTGCAGGATTTATGAGTATTGTTCCTGAAAAGTTTATTAATAAGTGGTTATATAAGGTTATTAATAT
ACATCCTTCTCTCTTGCCATCATTTAAGGGTTTAAATGCACAAGCTCAAGCATTAAAGGCTGGAGTAAAGATTGCTGGAT
GTACAGTTCATTATGTATATCCAGAAGTTGATGGTGGACCTATTATTGTTCAGGCAGCAGTTCCAGTGTTTTCATCTGAT
AGTGTTGAGGATCTTGCTAATAGAATATTGAAGATGGAACATATTTGTTACCCTAAAGCTGTGGAACTAATTGCGTATAA
TCAGCTACAACTTAACGGTAGTTTAGCTTTATCAGCAAAAACACTACACATGTTTTATAATGATGAAGCTTTTGTATAG

Upstream 100 bases:

>100_bases
TAGTAGGCTGATTTTTTGGTATTGTGATTGATTTATGAGTGTGCTATTGTGGTATAAGTTTATATTTGTAGTTATATTTA
TATTTATATAGGTTCAGTTA

Downstream 100 bases:

>100_bases
CTTGTTTTTTTATGTATTGAACCTTATTTTTGGATAAGTTGGTGAACAATCTACTTTTCCTCGTTATAATACTGTAAGCA
ACTATTTCTTTGCCTTTGTT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTMKPLRLGILISGRGSNMQALINACQRDDFPASVSCVISNKSNANGLILAQQSNIKTFIVQGRPLDFDAIDNILEEHEV
DLICLAGFMSIVPEKFINKWLYKVINIHPSLLPSFKGLNAQAQALKAGVKIAGCTVHYVYPEVDGGPIIVQAAVPVFSSD
SVEDLANRILKMEHICYPKAVELIAYNQLQLNGSLALSAKTLHMFYNDEAFV

Sequences:

>Translated_212_residues
MTMKPLRLGILISGRGSNMQALINACQRDDFPASVSCVISNKSNANGLILAQQSNIKTFIVQGRPLDFDAIDNILEEHEV
DLICLAGFMSIVPEKFINKWLYKVINIHPSLLPSFKGLNAQAQALKAGVKIAGCTVHYVYPEVDGGPIIVQAAVPVFSSD
SVEDLANRILKMEHICYPKAVELIAYNQLQLNGSLALSAKTLHMFYNDEAFV
>Mature_211_residues
TMKPLRLGILISGRGSNMQALINACQRDDFPASVSCVISNKSNANGLILAQQSNIKTFIVQGRPLDFDAIDNILEEHEVD
LICLAGFMSIVPEKFINKWLYKVINIHPSLLPSFKGLNAQAQALKAGVKIAGCTVHYVYPEVDGGPIIVQAAVPVFSSDS
VEDLANRILKMEHICYPKAVELIAYNQLQLNGSLALSAKTLHMFYNDEAFV

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=194, Percent_Identity=46.9072164948454, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI209869995, Length=194, Percent_Identity=46.9072164948454, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI209869993, Length=194, Percent_Identity=46.9072164948454, Blast_Score=179, Evalue=2e-45,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=40.9326424870466, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1787483, Length=163, Percent_Identity=28.8343558282209, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=190, Percent_Identity=40, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6320616, Length=204, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582400, Length=192, Percent_Identity=44.2708333333333, Blast_Score=156, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23247; Mature: 23116

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMKPLRLGILISGRGSNMQALINACQRDDFPASVSCVISNKSNANGLILAQQSNIKTFI
CCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCCEEEE
VQGRPLDFDAIDNILEEHEVDLICLAGFMSIVPEKFINKWLYKVINIHPSLLPSFKGLNA
EECCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCH
QAQALKAGVKIAGCTVHYVYPEVDGGPIIVQAAVPVFSSDSVEDLANRILKMEHICYPKA
HHHHHHCCCEEEEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHH
VELIAYNQLQLNGSLALSAKTLHMFYNDEAFV
HHEEEEEEEEECCEEEEEEEEEEEEECCCCCC
>Mature Secondary Structure 
TMKPLRLGILISGRGSNMQALINACQRDDFPASVSCVISNKSNANGLILAQQSNIKTFI
CCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCCEEEE
VQGRPLDFDAIDNILEEHEVDLICLAGFMSIVPEKFINKWLYKVINIHPSLLPSFKGLNA
EECCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCH
QAQALKAGVKIAGCTVHYVYPEVDGGPIIVQAAVPVFSSDSVEDLANRILKMEHICYPKA
HHHHHHCCCEEEEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHH
VELIAYNQLQLNGSLALSAKTLHMFYNDEAFV
HHEEEEEEEEECCEEEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]