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The map label for this gene is priA [H]

Identifier: 58617278

GI number: 58617278

Start: 897709

End: 899703

Strand: Reverse

Name: priA [H]

Synonym: ERGA_CDS_05510

Alternate gene names: 58617278

Gene position: 899703-897709 (Counterclockwise)

Preceding gene: 58617279

Following gene: 58617274

Centisome position: 59.98

GC content: 30.43

Gene sequence:

>1995_bases
GTGAATGTGTCAGGCATGATTGCTGAAGTTTTGCTACCAGTGCCGGTTAATAAAACGTTTTTTTATAATATTCCTTATAA
TGAGAACTTTATTGTAGGAGATTATGTTATTGTACCTTTCGGGTCTAGATTGATTGTAGGTATTGTGTTAGCTATCAGTG
ATTCTATACCTTCAACTTGTGAAGTAAATATTTCACAGTTAAAAATGGTAAATTGCAAAGGTCCTTTGCCTGGAATTTGT
AGTGCACTTATTCATTTTATAAAGTGGGTTAGTGATTATAATGTTGTTCCTGCTGGTTTAATTTTAAAAATGGTTTTTAG
TAATGTAGTAAATGTTAAATTTTTTAATAAACTTCAATTTTCTGTTGATGAAAATAAAAAGTATAGTAGTGACATAAAAA
TTAATTTATCTGATGCACAAAAACAAGCGTATAATAATATTATTGGTAAGATATCAAGTTATTCAGTCATTCTTTTAGAT
GGAGAGACTGGTTCTGGGAAGACAGAAGTTTATTGTGAAGTTATACGTGAATTAGTTACTAGAGATAATACAGCACAGGT
ATTAGTTTTGCTTCCAGAAATAGTATTAACATTACAGTTGATTAAACGAATAAAATATTATTTCGCAAACTATTTTCCAG
TGGAATGGCATTCAAATTTAACAGTAAAAAGTCGTAGAGAGTATTGGTTATCAATAGCATATGGTCAGTCTTCAATTGTG
ATAGGAGCAAGATCTGCGTTATTTCTACCTTTTAAAAATCTTAAAATGATTATTGTTGATGAAGAACATGATGCATCATT
TAAACAAGATTATGGTGTAATATATAATGCTCGTGATATGTCGATAGTTCTAGCAAAGCAGTTTAATATACCAGTTATTT
TATCTTCGGCTACTCCATCTTTAGAAACCATTAGTAATATACAAAAATCTCAGTATTATCATGTGAGGTTAACTCAAAGG
TTTGGAGATGCTAGGTTGCCTACGGTAAAGATTATTGATTTGTGTAAAAGCAAAATGGTGAATAGGTGGTTATCTTATGA
TCTTTACAGTAGTATGCTGGAGGTATTGCAAAGGCAAGATCAAGTTATGCTGTTTTTAAATCGTCGTGGGTATGCAACAT
TAAGATTATGTAAAACTTGTGGATTTAAAGTTCAATGTAAAAATTGTGCTACATGGCTTATAGAACATAAAAGGAGGAAT
GTATTATTGTGCCATTATTGTGGATATTCATGTACAATAGTAGATGAATGTTACAATTGTTTAGATAAATCATCAGTTAT
ATCTTATGGGATAGGAGTAGAAAAAGTAGCGGAAGATATAATGACACTCATACCAGATGCTAAAATTGCAATAATAAGCA
GTGATATTAGTAGTAAGGAAATTGGTGTGACTATTGATCTTATTATGCAAAATAAGGTAAACATTATTATTGGAACTCAA
GTAATAGCAAAAGGTCATAATTTTCCCAAATTGACATTAGTTGGTGTAATAGATGCTGATTTAGGTTTGAATAATAGTGA
TTTACGTGCGACAGAGAGAACATATCAGTTATTACATCAGGTAGCAGGACGTTCTGGTAGATTTGTCGATAGAAGTGAGG
TAATTTTACAGACCTATGATGCAAATAATGCTTTGATTAAATCTTTATCATCTTATAATCGTGAGTTGTTTTATGATTTG
GAACTAAAATCTAGGTTATTAACTCAAATGCCACCTTATACTAGATTAATAGGTATTATAGTTACTGGAAAAAATGAAGC
TAAAGTAATCAATGTGTCTCAACAAATAGTGAAAAGTCTTCCAACAACACTTACCGTACTTGGTCCTGCTCCTGCTCCGA
TTAGTTTGTTAAATAGACAATACAGGTATAGGATTTTAATTAAAGTACAAAATAATGTTGTAATACAAAAGCTACTTACT
AGATATAAAGAATATTATGCAAGTACGGGTAAGGTAAAAATTATTATCGATGTTGATCCAATTAATTTTATGTGA

Upstream 100 bases:

>100_bases
AGGTAATTCAGAAGTTATATCACCTTAAATACATTAGTATAGTTAATAGTGTGAATATTTTTATTTTTCTGCTATAATCT
GCTGTAAATCAGTATTTTCT

Downstream 100 bases:

>100_bases
GTTTTGTATTATTGTAGGGGGGGGGGGTTATTTTTGCTAAGGATTAAATCACATAATTTTAATTATATATAAAGCTAGTT
AATATTCAGGGTGTTCATAA

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MNVSGMIAEVLLPVPVNKTFFYNIPYNENFIVGDYVIVPFGSRLIVGIVLAISDSIPSTCEVNISQLKMVNCKGPLPGIC
SALIHFIKWVSDYNVVPAGLILKMVFSNVVNVKFFNKLQFSVDENKKYSSDIKINLSDAQKQAYNNIIGKISSYSVILLD
GETGSGKTEVYCEVIRELVTRDNTAQVLVLLPEIVLTLQLIKRIKYYFANYFPVEWHSNLTVKSRREYWLSIAYGQSSIV
IGARSALFLPFKNLKMIIVDEEHDASFKQDYGVIYNARDMSIVLAKQFNIPVILSSATPSLETISNIQKSQYYHVRLTQR
FGDARLPTVKIIDLCKSKMVNRWLSYDLYSSMLEVLQRQDQVMLFLNRRGYATLRLCKTCGFKVQCKNCATWLIEHKRRN
VLLCHYCGYSCTIVDECYNCLDKSSVISYGIGVEKVAEDIMTLIPDAKIAIISSDISSKEIGVTIDLIMQNKVNIIIGTQ
VIAKGHNFPKLTLVGVIDADLGLNNSDLRATERTYQLLHQVAGRSGRFVDRSEVILQTYDANNALIKSLSSYNRELFYDL
ELKSRLLTQMPPYTRLIGIIVTGKNEAKVINVSQQIVKSLPTTLTVLGPAPAPISLLNRQYRYRILIKVQNNVVIQKLLT
RYKEYYASTGKVKIIIDVDPINFM

Sequences:

>Translated_664_residues
MNVSGMIAEVLLPVPVNKTFFYNIPYNENFIVGDYVIVPFGSRLIVGIVLAISDSIPSTCEVNISQLKMVNCKGPLPGIC
SALIHFIKWVSDYNVVPAGLILKMVFSNVVNVKFFNKLQFSVDENKKYSSDIKINLSDAQKQAYNNIIGKISSYSVILLD
GETGSGKTEVYCEVIRELVTRDNTAQVLVLLPEIVLTLQLIKRIKYYFANYFPVEWHSNLTVKSRREYWLSIAYGQSSIV
IGARSALFLPFKNLKMIIVDEEHDASFKQDYGVIYNARDMSIVLAKQFNIPVILSSATPSLETISNIQKSQYYHVRLTQR
FGDARLPTVKIIDLCKSKMVNRWLSYDLYSSMLEVLQRQDQVMLFLNRRGYATLRLCKTCGFKVQCKNCATWLIEHKRRN
VLLCHYCGYSCTIVDECYNCLDKSSVISYGIGVEKVAEDIMTLIPDAKIAIISSDISSKEIGVTIDLIMQNKVNIIIGTQ
VIAKGHNFPKLTLVGVIDADLGLNNSDLRATERTYQLLHQVAGRSGRFVDRSEVILQTYDANNALIKSLSSYNRELFYDL
ELKSRLLTQMPPYTRLIGIIVTGKNEAKVINVSQQIVKSLPTTLTVLGPAPAPISLLNRQYRYRILIKVQNNVVIQKLLT
RYKEYYASTGKVKIIIDVDPINFM
>Mature_664_residues
MNVSGMIAEVLLPVPVNKTFFYNIPYNENFIVGDYVIVPFGSRLIVGIVLAISDSIPSTCEVNISQLKMVNCKGPLPGIC
SALIHFIKWVSDYNVVPAGLILKMVFSNVVNVKFFNKLQFSVDENKKYSSDIKINLSDAQKQAYNNIIGKISSYSVILLD
GETGSGKTEVYCEVIRELVTRDNTAQVLVLLPEIVLTLQLIKRIKYYFANYFPVEWHSNLTVKSRREYWLSIAYGQSSIV
IGARSALFLPFKNLKMIIVDEEHDASFKQDYGVIYNARDMSIVLAKQFNIPVILSSATPSLETISNIQKSQYYHVRLTQR
FGDARLPTVKIIDLCKSKMVNRWLSYDLYSSMLEVLQRQDQVMLFLNRRGYATLRLCKTCGFKVQCKNCATWLIEHKRRN
VLLCHYCGYSCTIVDECYNCLDKSSVISYGIGVEKVAEDIMTLIPDAKIAIISSDISSKEIGVTIDLIMQNKVNIIIGTQ
VIAKGHNFPKLTLVGVIDADLGLNNSDLRATERTYQLLHQVAGRSGRFVDRSEVILQTYDANNALIKSLSSYNRELFYDL
ELKSRLLTQMPPYTRLIGIIVTGKNEAKVINVSQQIVKSLPTTLTVLGPAPAPISLLNRQYRYRILIKVQNNVVIQKLLT
RYKEYYASTGKVKIIIDVDPINFM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=557, Percent_Identity=36.983842010772, Blast_Score=353, Evalue=1e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 75101; Mature: 75101

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSGMIAEVLLPVPVNKTFFYNIPYNENFIVGDYVIVPFGSRLIVGIVLAISDSIPSTC
CCCCHHHHHHEECCCCCCEEEEECCCCCCEEEECEEEECCCCHHHHHHHEEECCCCCCCE
EVNISQLKMVNCKGPLPGICSALIHFIKWVSDYNVVPAGLILKMVFSNVVNVKFFNKLQF
EEEHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEEEEEE
SVDENKKYSSDIKINLSDAQKQAYNNIIGKISSYSVILLDGETGSGKTEVYCEVIRELVT
EECCCCCCCCEEEEEECHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHC
RDNTAQVLVLLPEIVLTLQLIKRIKYYFANYFPVEWHSNLTVKSRREYWLSIAYGQSSIV
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCEEEEEEEECCCEEE
IGARSALFLPFKNLKMIIVDEEHDASFKQDYGVIYNARDMSIVLAKQFNIPVILSSATPS
EEECCEEEEEECCCEEEEEECCCCCCCCCCCCEEEECCCCEEEEEECCCCCEEEECCCCC
LETISNIQKSQYYHVRLTQRFGDARLPTVKIIDLCKSKMVNRWLSYDLYSSMLEVLQRQD
HHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QVMLFLNRRGYATLRLCKTCGFKVQCKNCATWLIEHKRRNVLLCHYCGYSCTIVDECYNC
CEEEEEECCCCEEEHHHHHCCCEEEECHHHHHHHHHCCCCEEEEEECCCEEEEHHHHHHH
LDKSSVISYGIGVEKVAEDIMTLIPDAKIAIISSDISSKEIGVTIDLIMQNKVNIIIGTQ
HCCCCHHEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCEEEEEECE
VIAKGHNFPKLTLVGVIDADLGLNNSDLRATERTYQLLHQVAGRSGRFVDRSEVILQTYD
EEECCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
ANNALIKSLSSYNRELFYDLELKSRLLTQMPPYTRLIGIIVTGKNEAKVINVSQQIVKSL
CCHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCHHEEEEEEECCCCCEEEEHHHHHHHHC
PTTLTVLGPAPAPISLLNRQYRYRILIKVQNNVVIQKLLTRYKEYYASTGKVKIIIDVDP
CCEEEEECCCCCCHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECC
INFM
CCCC
>Mature Secondary Structure
MNVSGMIAEVLLPVPVNKTFFYNIPYNENFIVGDYVIVPFGSRLIVGIVLAISDSIPSTC
CCCCHHHHHHEECCCCCCEEEEECCCCCCEEEECEEEECCCCHHHHHHHEEECCCCCCCE
EVNISQLKMVNCKGPLPGICSALIHFIKWVSDYNVVPAGLILKMVFSNVVNVKFFNKLQF
EEEHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEEEEEE
SVDENKKYSSDIKINLSDAQKQAYNNIIGKISSYSVILLDGETGSGKTEVYCEVIRELVT
EECCCCCCCCEEEEEECHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHC
RDNTAQVLVLLPEIVLTLQLIKRIKYYFANYFPVEWHSNLTVKSRREYWLSIAYGQSSIV
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCEEEEEEEECCCEEE
IGARSALFLPFKNLKMIIVDEEHDASFKQDYGVIYNARDMSIVLAKQFNIPVILSSATPS
EEECCEEEEEECCCEEEEEECCCCCCCCCCCCEEEECCCCEEEEEECCCCCEEEECCCCC
LETISNIQKSQYYHVRLTQRFGDARLPTVKIIDLCKSKMVNRWLSYDLYSSMLEVLQRQD
HHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QVMLFLNRRGYATLRLCKTCGFKVQCKNCATWLIEHKRRNVLLCHYCGYSCTIVDECYNC
CEEEEEECCCCEEEHHHHHCCCEEEECHHHHHHHHHCCCCEEEEEECCCEEEEHHHHHHH
LDKSSVISYGIGVEKVAEDIMTLIPDAKIAIISSDISSKEIGVTIDLIMQNKVNIIIGTQ
HCCCCHHEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCEEEEEECE
VIAKGHNFPKLTLVGVIDADLGLNNSDLRATERTYQLLHQVAGRSGRFVDRSEVILQTYD
EEECCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
ANNALIKSLSSYNRELFYDLELKSRLLTQMPPYTRLIGIIVTGKNEAKVINVSQQIVKSL
CCHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCHHEEEEEEECCCCCEEEEHHHHHHHHC
PTTLTVLGPAPAPISLLNRQYRYRILIKVQNNVVIQKLLTRYKEYYASTGKVKIIIDVDP
CCEEEEECCCCCCHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECC
INFM
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]