| Definition | Ehrlichia ruminantium str. Gardel, complete genome. |
|---|---|
| Accession | NC_006831 |
| Length | 1,499,920 |
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The map label for this gene is nadE [H]
Identifier: 58616998
GI number: 58616998
Start: 448747
End: 450288
Strand: Reverse
Name: nadE [H]
Synonym: ERGA_CDS_02710
Alternate gene names: 58616998
Gene position: 450288-448747 (Counterclockwise)
Preceding gene: 58616999
Following gene: 58616996
Centisome position: 30.02
GC content: 27.5
Gene sequence:
>1542_bases ATGAATAAGTTATCAATTTTTATTTCTCAACTCAATTATATACCAGAAGATCCTAATTATAATTATAATAAAATCTTAAA ATCATATAACAAATCAACAAGTTTATCAGCTGATATAGCAATATTTTCACGATATGCAATTTCAGGATATATACAAAACA TTCCAACTTTATCCAAAGACTTTTTTAATCAATGCTCAAATATTATTGAAAAATTAGCAATACATACCAAAAAACATAAT ACAGCAATACTTATTGGTAGTATAAAAGAACTTAACAATATAATACACGAAACTATTTACTTAATAAAAAATGGTATAAT TACAGAACTTGTAACATTTATAACAAATACTTTACCTGCTATTATTCCTACTACTTTTTCAATCAATAAAACAATATTTA CACTATACCCTATATCAGAAGAATTAGAATTACCCATACATTCAGAAAATACTATATTATTAATCATGGATAATACTCCA TATACTCAAAATACCAATATACGACATGAGGCAATTGTAAAACTAACACACAACAAACATGTAGTATATATAAACCAAAT GGGTGGGTATAATAGTGTAGTTTTTCATGGAAAATCCTTTTTTACTTTTAATAATCAATATAACTTTTTAAATACATGGG AAGAAGATAGTAAAATTTTCAAAGCAGATTCAAGAAAAAGTAACTATAACAATGCTGTTGTACATAGCGATAATAATATT AGATCAGATTATCAAGCACTGATGCTTGCATTAAAAGATTATGTACAAAAAAATTCTATGACCAAAGTGATACTAGGGTT ATCAGGAGGAATAGATTCTGCACTTGTAGCAACAATTGCTGCAGATGGATTAGGACCAGCCAATGTTCTTTCATTTATGT TACCAACAAAATATACATCAGAAACTAGCATGATTGACGCTCAAGAGTGTGCTGCAAGATTAGGAATTTCATATAATATA TTATCGATAGAAACAATATTTCAAACTTCACTCACAATTCTTAACAACATTTTTGCTGGCACACAAGAAGATATAACCGA AGAAAACTTACAATCCAGAATTCGGGGATTAATCTTGATGGCATTAAGTAATAAATTTAAACTGATGTTATTATCAACAG GTAATAAATCAGAATCATTAGTCGGATATGCAACACTATATGGAGATATGTGTGGAGGATTTGCTCCAATAAAAGATATA TACAAAACTAGGGTATATGAATTAAGTCAATGGAGAAACAAAAACATACCTATAAATAGTTTATGTAAGAATACAGATAT TATTCCATACCGTATAATAACAAAACATCCATCTGCTGAACTACGTTACAATCAAATAGATCAAGATACATTACCTGCTT ATGCTATATTAGACTCAATTCTCACATTATTAGTTGATCAACGTCTTTCTATAGAAGAAGTTATTAATAAAGGTCATAGT AGTAAAAATGTAAATTATGTAGCACATTTAATCAAAAAATCTGAATTCAAAAGGAAACAAGCCCCTATAGGATTAATAAT AACTCATCCACATAATAAATGA
Upstream 100 bases:
>100_bases ATAATATTATCATAGATATATTTTTCTGCAGTATAGAAACTATAATAAACTACTGTAATAAATACATACTAAATATTAAC ACTAAACTAAGCCATAATTT
Downstream 100 bases:
>100_bases CTTCTCAAGTAAAATAATAGTTTTAAATAACACGTATACAGTAGATTTTAACAAAAACATATCATAGCACTATGTAACAC AAATAATACTTAATTTTTTT
Product: putative glutamine-dependent NAD(+) synthetase
Products: NA
Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]
Number of amino acids: Translated: 513; Mature: 513
Protein sequence:
>513_residues MNKLSIFISQLNYIPEDPNYNYNKILKSYNKSTSLSADIAIFSRYAISGYIQNIPTLSKDFFNQCSNIIEKLAIHTKKHN TAILIGSIKELNNIIHETIYLIKNGIITELVTFITNTLPAIIPTTFSINKTIFTLYPISEELELPIHSENTILLIMDNTP YTQNTNIRHEAIVKLTHNKHVVYINQMGGYNSVVFHGKSFFTFNNQYNFLNTWEEDSKIFKADSRKSNYNNAVVHSDNNI RSDYQALMLALKDYVQKNSMTKVILGLSGGIDSALVATIAADGLGPANVLSFMLPTKYTSETSMIDAQECAARLGISYNI LSIETIFQTSLTILNNIFAGTQEDITEENLQSRIRGLILMALSNKFKLMLLSTGNKSESLVGYATLYGDMCGGFAPIKDI YKTRVYELSQWRNKNIPINSLCKNTDIIPYRIITKHPSAELRYNQIDQDTLPAYAILDSILTLLVDQRLSIEEVINKGHS SKNVNYVAHLIKKSEFKRKQAPIGLIITHPHNK
Sequences:
>Translated_513_residues MNKLSIFISQLNYIPEDPNYNYNKILKSYNKSTSLSADIAIFSRYAISGYIQNIPTLSKDFFNQCSNIIEKLAIHTKKHN TAILIGSIKELNNIIHETIYLIKNGIITELVTFITNTLPAIIPTTFSINKTIFTLYPISEELELPIHSENTILLIMDNTP YTQNTNIRHEAIVKLTHNKHVVYINQMGGYNSVVFHGKSFFTFNNQYNFLNTWEEDSKIFKADSRKSNYNNAVVHSDNNI RSDYQALMLALKDYVQKNSMTKVILGLSGGIDSALVATIAADGLGPANVLSFMLPTKYTSETSMIDAQECAARLGISYNI LSIETIFQTSLTILNNIFAGTQEDITEENLQSRIRGLILMALSNKFKLMLLSTGNKSESLVGYATLYGDMCGGFAPIKDI YKTRVYELSQWRNKNIPINSLCKNTDIIPYRIITKHPSAELRYNQIDQDTLPAYAILDSILTLLVDQRLSIEEVINKGHS SKNVNYVAHLIKKSEFKRKQAPIGLIITHPHNK >Mature_513_residues MNKLSIFISQLNYIPEDPNYNYNKILKSYNKSTSLSADIAIFSRYAISGYIQNIPTLSKDFFNQCSNIIEKLAIHTKKHN TAILIGSIKELNNIIHETIYLIKNGIITELVTFITNTLPAIIPTTFSINKTIFTLYPISEELELPIHSENTILLIMDNTP YTQNTNIRHEAIVKLTHNKHVVYINQMGGYNSVVFHGKSFFTFNNQYNFLNTWEEDSKIFKADSRKSNYNNAVVHSDNNI RSDYQALMLALKDYVQKNSMTKVILGLSGGIDSALVATIAADGLGPANVLSFMLPTKYTSETSMIDAQECAARLGISYNI LSIETIFQTSLTILNNIFAGTQEDITEENLQSRIRGLILMALSNKFKLMLLSTGNKSESLVGYATLYGDMCGGFAPIKDI YKTRVYELSQWRNKNIPINSLCKNTDIIPYRIITKHPSAELRYNQIDQDTLPAYAILDSILTLLVDQRLSIEEVINKGHS SKNVNYVAHLIKKSEFKRKQAPIGLIITHPHNK
Specific function: Can use both glutamine or ammonia as a nitrogen source [H]
COG id: COG0171
COG function: function code H; NAD synthase
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 CN hydrolase domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014445 - InterPro: IPR022310 - InterPro: IPR003694 - InterPro: IPR000132 - InterPro: IPR003010 - InterPro: IPR014729 [H]
Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]
EC number: =6.3.5.1 [H]
Molecular weight: Translated: 57948; Mature: 57948
Theoretical pI: Translated: 8.84; Mature: 8.84
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNKLSIFISQLNYIPEDPNYNYNKILKSYNKSTSLSADIAIFSRYAISGYIQNIPTLSKD CCHHHEEHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH FFNQCSNIIEKLAIHTKKHNTAILIGSIKELNNIIHETIYLIKNGIITELVTFITNTLPA HHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH IIPTTFSINKTIFTLYPISEELELPIHSENTILLIMDNTPYTQNTNIRHEAIVKLTHNKH HHCCCEEECCEEEEEEECCCCCCCCEECCCEEEEEECCCCCCCCCCCCCEEEEEEECCCE VVYINQMGGYNSVVFHGKSFFTFNNQYNFLNTWEEDSKIFKADSRKSNYNNAVVHSDNNI EEEEECCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCEEEECCCCC RSDYQALMLALKDYVQKNSMTKVILGLSGGIDSALVATIAADGLGPANVLSFMLPTKYTS HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCC ETSMIDAQECAARLGISYNILSIETIFQTSLTILNNIFAGTQEDITEENLQSRIRGLILM CCCCCCHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEHE ALSNKFKLMLLSTGNKSESLVGYATLYGDMCGGFAPIKDIYKTRVYELSQWRNKNIPINS EECCCEEEEEEECCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH LCKNTDIIPYRIITKHPSAELRYNQIDQDTLPAYAILDSILTLLVDQRLSIEEVINKGHS HHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCC SKNVNYVAHLIKKSEFKRKQAPIGLIITHPHNK CCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCC >Mature Secondary Structure MNKLSIFISQLNYIPEDPNYNYNKILKSYNKSTSLSADIAIFSRYAISGYIQNIPTLSKD CCHHHEEHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH FFNQCSNIIEKLAIHTKKHNTAILIGSIKELNNIIHETIYLIKNGIITELVTFITNTLPA HHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH IIPTTFSINKTIFTLYPISEELELPIHSENTILLIMDNTPYTQNTNIRHEAIVKLTHNKH HHCCCEEECCEEEEEEECCCCCCCCEECCCEEEEEECCCCCCCCCCCCCEEEEEEECCCE VVYINQMGGYNSVVFHGKSFFTFNNQYNFLNTWEEDSKIFKADSRKSNYNNAVVHSDNNI EEEEECCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCEEEECCCCC RSDYQALMLALKDYVQKNSMTKVILGLSGGIDSALVATIAADGLGPANVLSFMLPTKYTS HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCC ETSMIDAQECAARLGISYNILSIETIFQTSLTILNNIFAGTQEDITEENLQSRIRGLILM CCCCCCHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEHE ALSNKFKLMLLSTGNKSESLVGYATLYGDMCGGFAPIKDIYKTRVYELSQWRNKNIPINS EECCCEEEEEEECCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH LCKNTDIIPYRIITKHPSAELRYNQIDQDTLPAYAILDSILTLLVDQRLSIEEVINKGHS HHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCC SKNVNYVAHLIKKSEFKRKQAPIGLIITHPHNK CCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10910347 [H]