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The map label for this gene is hemB [H]

Identifier: 58616999

GI number: 58616999

Start: 451400

End: 452389

Strand: Reverse

Name: hemB [H]

Synonym: ERGA_CDS_02720

Alternate gene names: 58616999

Gene position: 452389-451400 (Counterclockwise)

Preceding gene: 58617002

Following gene: 58616998

Centisome position: 30.16

GC content: 32.32

Gene sequence:

>990_bases
ATGCAATTTCCTACCACAAGACTAAGACGTAAACGCTCACATCAATGGTTACGCAATCTAGTAAGAGAAAATAATCTATC
TGTAAATGATCTAATTCTTCCCTTATTCGTTCATGATGATGAAGGAGTATCAGAACCCATAAAACAATTACCAGATATTA
AAAGATATTCTATTCCTGAATTGTTAAATGTAGTAGCACAAGCTGCAGATTTAGGAATAAACGCAATAGCACTATTTCCA
GTAGTAAAAAACAATTTAAAATCTGAAAAGGCAGAGGAAGCGTTTAACCCTAACAATCTCATATGTAGAGCAATAAAAAT
ATTGAAAGACAACATTCCAAAAATAGGCATAATTGCCGATGTAGCACTAGATCCTTACACAATTTCAGGTCATGATGGAA
TTATTATAGATCAAAAAATTGATAATGATGCAACTGTAGCAACTTTATGTAAGCAAGCTTTAGTATTAGCAGCAGCAGGA
TGTGATATTATAGCACCTTCAGATATGATGGATGGTAGAATTAAAGAAATTAGAAACTCACTAGATAACTGTAATTTTCA
AGACATCTGTATCTTATCTTACGCAGTAAAATATTGCTCAAGTTTTTACAAACCATTTCGTGAAGCTGTTGGGTCATGCA
AAACATCTAAGACTATAGACAAAAGTACTTATCAAGTAGATGCTGGAAATATCAACGAAGCACTTATTGAAGTACAAATG
GACATTAATGAAGGAGCAGATATTGTTATGATAAAACCAGGAATGCCATATCTTGATGTGATCAAATCTGTTAGTCAAAA
ATATGAAATCCCCATATTTGCATACCAAGTAAGTGGTGAATACGCAATGATTAAAGTTGCCTCACAAAACCATTTATTAG
ATTACGATCAAGCTATATATGAATCTTTATTAAGCTTTAAACGTGCAGGAGCAAAAAGTATACTGACATATGCAGCTATG
GATGTAGCTCTTATGATTTTAAATAATTAA

Upstream 100 bases:

>100_bases
CTGTTAAGTATATTCCAGACTCATTGTCATTTTGAAAGAATAATATTGATATTCAGTATCAAAAAAAATAACATACCTGT
ATAACAAAAAATAGAACAAT

Downstream 100 bases:

>100_bases
TTCTCAGATATACCACATGTATTATTTAGTAATTCTATAAGAACATTTAGAGTACAATTCATTATAGCTACACTACAAAA
CTATCCATTTAAATTACAAT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MQFPTTRLRRKRSHQWLRNLVRENNLSVNDLILPLFVHDDEGVSEPIKQLPDIKRYSIPELLNVVAQAADLGINAIALFP
VVKNNLKSEKAEEAFNPNNLICRAIKILKDNIPKIGIIADVALDPYTISGHDGIIIDQKIDNDATVATLCKQALVLAAAG
CDIIAPSDMMDGRIKEIRNSLDNCNFQDICILSYAVKYCSSFYKPFREAVGSCKTSKTIDKSTYQVDAGNINEALIEVQM
DINEGADIVMIKPGMPYLDVIKSVSQKYEIPIFAYQVSGEYAMIKVASQNHLLDYDQAIYESLLSFKRAGAKSILTYAAM
DVALMILNN

Sequences:

>Translated_329_residues
MQFPTTRLRRKRSHQWLRNLVRENNLSVNDLILPLFVHDDEGVSEPIKQLPDIKRYSIPELLNVVAQAADLGINAIALFP
VVKNNLKSEKAEEAFNPNNLICRAIKILKDNIPKIGIIADVALDPYTISGHDGIIIDQKIDNDATVATLCKQALVLAAAG
CDIIAPSDMMDGRIKEIRNSLDNCNFQDICILSYAVKYCSSFYKPFREAVGSCKTSKTIDKSTYQVDAGNINEALIEVQM
DINEGADIVMIKPGMPYLDVIKSVSQKYEIPIFAYQVSGEYAMIKVASQNHLLDYDQAIYESLLSFKRAGAKSILTYAAM
DVALMILNN
>Mature_329_residues
MQFPTTRLRRKRSHQWLRNLVRENNLSVNDLILPLFVHDDEGVSEPIKQLPDIKRYSIPELLNVVAQAADLGINAIALFP
VVKNNLKSEKAEEAFNPNNLICRAIKILKDNIPKIGIIADVALDPYTISGHDGIIIDQKIDNDATVATLCKQALVLAAAG
CDIIAPSDMMDGRIKEIRNSLDNCNFQDICILSYAVKYCSSFYKPFREAVGSCKTSKTIDKSTYQVDAGNINEALIEVQM
DINEGADIVMIKPGMPYLDVIKSVSQKYEIPIFAYQVSGEYAMIKVASQNHLLDYDQAIYESLLSFKRAGAKSILTYAAM
DVALMILNN

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=319, Percent_Identity=34.7962382445141, Blast_Score=198, Evalue=5e-51,
Organism=Escherichia coli, GI87081728, Length=317, Percent_Identity=40.0630914826498, Blast_Score=238, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6321398, Length=312, Percent_Identity=34.9358974358974, Blast_Score=182, Evalue=6e-47,
Organism=Drosophila melanogaster, GI21358291, Length=313, Percent_Identity=34.185303514377, Blast_Score=207, Evalue=9e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36621; Mature: 36621

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFPTTRLRRKRSHQWLRNLVRENNLSVNDLILPLFVHDDEGVSEPIKQLPDIKRYSIPE
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCHHHHHHHCCCCCCCCHHH
LLNVVAQAADLGINAIALFPVVKNNLKSEKAEEAFNPNNLICRAIKILKDNIPKIGIIAD
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEE
VALDPYTISGHDGIIIDQKIDNDATVATLCKQALVLAAAGCDIIAPSDMMDGRIKEIRNS
ECCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHCHHHHHHHHH
LDNCNFQDICILSYAVKYCSSFYKPFREAVGSCKTSKTIDKSTYQVDAGNINEALIEVQM
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEEEEEEE
DINEGADIVMIKPGMPYLDVIKSVSQKYEIPIFAYQVSGEYAMIKVASQNHLLDYDQAIY
ECCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCHHHHHH
ESLLSFKRAGAKSILTYAAMDVALMILNN
HHHHHHHHCCHHHHHHHHHHHHHHEEECC
>Mature Secondary Structure
MQFPTTRLRRKRSHQWLRNLVRENNLSVNDLILPLFVHDDEGVSEPIKQLPDIKRYSIPE
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCHHHHHHHCCCCCCCCHHH
LLNVVAQAADLGINAIALFPVVKNNLKSEKAEEAFNPNNLICRAIKILKDNIPKIGIIAD
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEE
VALDPYTISGHDGIIIDQKIDNDATVATLCKQALVLAAAGCDIIAPSDMMDGRIKEIRNS
ECCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHCHHHHHHHHH
LDNCNFQDICILSYAVKYCSSFYKPFREAVGSCKTSKTIDKSTYQVDAGNINEALIEVQM
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEEEEEEE
DINEGADIVMIKPGMPYLDVIKSVSQKYEIPIFAYQVSGEYAMIKVASQNHLLDYDQAIY
ECCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCHHHHHH
ESLLSFKRAGAKSILTYAAMDVALMILNN
HHHHHHHHCCHHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]