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The map label for this gene is ispD [H]

Identifier: 58616823

GI number: 58616823

Start: 159569

End: 160297

Strand: Reverse

Name: ispD [H]

Synonym: ERGA_CDS_00960

Alternate gene names: 58616823

Gene position: 160297-159569 (Counterclockwise)

Preceding gene: 58616826

Following gene: 58616822

Centisome position: 10.69

GC content: 31.14

Gene sequence:

>729_bases
ATGTATCAGTTTGTATTACTAATTGTTGCAGCTGGCAATAGTAAGAGATTCAAAGGCAGTGAAAAACCCAAACAATATAT
GGAATTAGGTCATTATCCTATACTATATCATACAATCAACAACGTAATTACTACCCCTTATATTGGCTCTGTGAAAGTAG
TTATCAGACAAGAACATGAATACTTATATAATAGCTGTATAAAAAAAATACATAGTAGTAAGCTTTTACCATTCTCATAT
GGAGGAACAAGAAGGCAGGATTCCGTTCGCATTGGATTAGAAAGTATAAAATACCTTAATCCGGATTTTGTGATAATACA
TGACGCATGCCGTCCTTTTATTAGCACCACACTATTAGATAAAATGTTACCGCAATTAACCAACTATGTAGGAGTAATTC
CAGTATTATCGATTACTGAAACTATCCATATGATAAACGAAAACCATACTATCATTAAAAATATAAATCGAAACACATTA
AAATTAGTACAGACTCCTCAAATATATAGGTACACTGATATACTATCGTCTCATATGCTTTCCTATACCACCGACCCCAC
TAAAGAATTTCCAGATGAATCATCTCTAATGATACACTACAATTTTCCAATAGCAACAATTGAAGGTAGTAACTATAATT
TGAAAATTACAACAAATGACGATTTACATATAGCAACTTTATTATATAAATCTATGAATACTAAAGTTCCTAATCATACC
CTTAACTGA

Upstream 100 bases:

>100_bases
AAACTTATACAACTTAATAACTATTGCATAATTATAGTCAATACTTTAAAATTTACAGACTAGTAAATGAATAGAAAAAA
TCCACAAAATTAAAAAAACT

Downstream 100 bases:

>100_bases
GGGTAATACCACCATCATCAAATAATCCATAATATTTTCTAAAATTCACAATGCTATTTCTAATATTTTCATTTGACATC
ATAATCACCCAAGAATCGCT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MYQFVLLIVAAGNSKRFKGSEKPKQYMELGHYPILYHTINNVITTPYIGSVKVVIRQEHEYLYNSCIKKIHSSKLLPFSY
GGTRRQDSVRIGLESIKYLNPDFVIIHDACRPFISTTLLDKMLPQLTNYVGVIPVLSITETIHMINENHTIIKNINRNTL
KLVQTPQIYRYTDILSSHMLSYTTDPTKEFPDESSLMIHYNFPIATIEGSNYNLKITTNDDLHIATLLYKSMNTKVPNHT
LN

Sequences:

>Translated_242_residues
MYQFVLLIVAAGNSKRFKGSEKPKQYMELGHYPILYHTINNVITTPYIGSVKVVIRQEHEYLYNSCIKKIHSSKLLPFSY
GGTRRQDSVRIGLESIKYLNPDFVIIHDACRPFISTTLLDKMLPQLTNYVGVIPVLSITETIHMINENHTIIKNINRNTL
KLVQTPQIYRYTDILSSHMLSYTTDPTKEFPDESSLMIHYNFPIATIEGSNYNLKITTNDDLHIATLLYKSMNTKVPNHT
LN
>Mature_242_residues
MYQFVLLIVAAGNSKRFKGSEKPKQYMELGHYPILYHTINNVITTPYIGSVKVVIRQEHEYLYNSCIKKIHSSKLLPFSY
GGTRRQDSVRIGLESIKYLNPDFVIIHDACRPFISTTLLDKMLPQLTNYVGVIPVLSITETIHMINENHTIIKNINRNTL
KLVQTPQIYRYTDILSSHMLSYTTDPTKEFPDESSLMIHYNFPIATIEGSNYNLKITTNDDLHIATLLYKSMNTKVPNHT
LN

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=229, Percent_Identity=25.764192139738, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 27854; Mature: 27854

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQFVLLIVAAGNSKRFKGSEKPKQYMELGHYPILYHTINNVITTPYIGSVKVVIRQEHE
CCEEEEEEEECCCCCCCCCCCCCHHHHHCCCCCEEEEEHHHHCCCCCCCCEEEEEEHHHH
YLYNSCIKKIHSSKLLPFSYGGTRRQDSVRIGLESIKYLNPDFVIIHDACRPFISTTLLD
HHHHHHHHHHHCCCCCEECCCCCCCCCCEEECHHHHHCCCCCEEEEHHHHHHHHHHHHHH
KMLPQLTNYVGVIPVLSITETIHMINENHTIIKNINRNTLKLVQTPQIYRYTDILSSHML
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHH
SYTTDPTKEFPDESSLMIHYNFPIATIEGSNYNLKITTNDDLHIATLLYKSMNTKVPNHT
HCCCCCCHHCCCCCCEEEEECCCEEEEECCCEEEEEEECCCEEHHHHHHHHHCCCCCCCC
LN
CC
>Mature Secondary Structure
MYQFVLLIVAAGNSKRFKGSEKPKQYMELGHYPILYHTINNVITTPYIGSVKVVIRQEHE
CCEEEEEEEECCCCCCCCCCCCCHHHHHCCCCCEEEEEHHHHCCCCCCCCEEEEEEHHHH
YLYNSCIKKIHSSKLLPFSYGGTRRQDSVRIGLESIKYLNPDFVIIHDACRPFISTTLLD
HHHHHHHHHHHCCCCCEECCCCCCCCCCEEECHHHHHCCCCCEEEEHHHHHHHHHHHHHH
KMLPQLTNYVGVIPVLSITETIHMINENHTIIKNINRNTLKLVQTPQIYRYTDILSSHML
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHH
SYTTDPTKEFPDESSLMIHYNFPIATIEGSNYNLKITTNDDLHIATLLYKSMNTKVPNHT
HCCCCCCHHCCCCCCEEEEECCCEEEEECCCEEEEEEECCCEEHHHHHHHHHCCCCCCCC
LN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA