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The map label for this gene is glnA [H]

Identifier: 58616778

GI number: 58616778

Start: 68778

End: 70190

Strand: Reverse

Name: glnA [H]

Synonym: ERGA_CDS_00510

Alternate gene names: 58616778

Gene position: 70190-68778 (Counterclockwise)

Preceding gene: 58616781

Following gene: 58616777

Centisome position: 4.68

GC content: 31.92

Gene sequence:

>1413_bases
ATGTTCCTTTCAGCAGAGGATACTCTAAACTACATAAAGAAAAATACAATAAATTTTGTTGATTTAAGGTTTACAGATTC
TACTAGTAGGTGGCATCATATAACACGTAATGCAGAAACAATAGATACATATGTTTTATCATCTGGAGTTAATTTTGACA
GTTCATCAATTCCAGGATGGCAATCAGTAGAGAAATCGGATATGATACTTCTACCAGATGTTAGTACTGCTTTTGTTGAC
CCATTTACAGCACAACCAACATTAGTAATAATCTGTAATGTAATAAACCCATATGACAAATCTGAATATCCTAAAGATCC
ACGTTATACAGCACATAAAGCATCACAATATATGTTATCTACTGGAATTGCTGACAAATGTTACTTTGGACCAGAAGTAG
AATTTTTTGTGTTTGATAATGCAACATTTTATAATGGAATGTATCATTCCTATTTTAAAATAGAATCTTCAGAACATATT
TCTCAATTTCAAAAAAGAGAACATATTAATAATAGTCACTGTCCAAAAATCAAGGGTGGCTATATGCCAACCCCTCCTGT
TGATTCATTACATGATCTCAGATCAGAAATACTAACAATGCTTAAGGAAGTGGGTATTACACCTTTAATACACCATCATG
AAGTTGCAGCTTCACAATGTGAAGTTGGATTCCAGCATGATGAGTTAATACGCAGTGCAGATAATGTACAAAAGTGCAAA
TATATAATCCACGGAGTAGCATCATCTTATGGAAAAACTGCAACTTTTATGCCAAAACCTGTTATAGGAGATAATGGAAG
TGGGATGCATTGTCATCAGTCCTTATGGAAAGATAACATTAACATTTTTGTCAACTCAGATGGGAGTATGTCAGAAATAT
GTTTATATTACATAGGTGGGATTATTAAATACGGGAAAGCTATTAATGCCTTTACAAATCCATCAACAAATAGCTATAAA
CGTTTAGTTCCTAATTTTGAGGCACCAACTTGGCTAACATACTCTTACGAGAATAGGTCATCTGCAATTCGTATCCCATA
TGTACCAAATACTGATCTTAATGCAAAAAGAATAGAAGTACGTTTTCCAGACCCACTTGCAAATCCATATCTATGCTTTT
CTGCACAATTAATGGCTGGTTTATATGGAATAAAAAATAAAATTCACCCAGGACCTTCAATAGATAAAAATTTATATACA
TTAGATAAAAAAGAACTTCAAAGTTTTATTTCTGTTGCAAATTCTCTAGAAGAATCTTTATATGCCTTAGACAATAATAG
AGAGTTCTTGTTAGAAGGAGATGTATTTACAAATGATCAAATTGACACATATATTAAATTAAAATCATCAGAAGCAAGAG
ACTTACATTTACATCCACATCCCATTGAATTCATAAATTATTATAGTTCTTAG

Upstream 100 bases:

>100_bases
AAAAGATGAACTTCTCTTTAAGGATACCATTATTACATTGCATATTGCAAAATAGTAAGAAACTTGATATTCTAAGGGTT
TTTAAGTTGGTAATTTAATC

Downstream 100 bases:

>100_bases
ACTTTCGATATACAAAAAATATGTTGAATACAAATTTATTTTAAATTTTAATGATAAAATTCTCACAGAAGAATTTTTAG
GTGCGATAGACAACAATATA

Product: glutamine synthetase I

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MFLSAEDTLNYIKKNTINFVDLRFTDSTSRWHHITRNAETIDTYVLSSGVNFDSSSIPGWQSVEKSDMILLPDVSTAFVD
PFTAQPTLVIICNVINPYDKSEYPKDPRYTAHKASQYMLSTGIADKCYFGPEVEFFVFDNATFYNGMYHSYFKIESSEHI
SQFQKREHINNSHCPKIKGGYMPTPPVDSLHDLRSEILTMLKEVGITPLIHHHEVAASQCEVGFQHDELIRSADNVQKCK
YIIHGVASSYGKTATFMPKPVIGDNGSGMHCHQSLWKDNINIFVNSDGSMSEICLYYIGGIIKYGKAINAFTNPSTNSYK
RLVPNFEAPTWLTYSYENRSSAIRIPYVPNTDLNAKRIEVRFPDPLANPYLCFSAQLMAGLYGIKNKIHPGPSIDKNLYT
LDKKELQSFISVANSLEESLYALDNNREFLLEGDVFTNDQIDTYIKLKSSEARDLHLHPHPIEFINYYSS

Sequences:

>Translated_470_residues
MFLSAEDTLNYIKKNTINFVDLRFTDSTSRWHHITRNAETIDTYVLSSGVNFDSSSIPGWQSVEKSDMILLPDVSTAFVD
PFTAQPTLVIICNVINPYDKSEYPKDPRYTAHKASQYMLSTGIADKCYFGPEVEFFVFDNATFYNGMYHSYFKIESSEHI
SQFQKREHINNSHCPKIKGGYMPTPPVDSLHDLRSEILTMLKEVGITPLIHHHEVAASQCEVGFQHDELIRSADNVQKCK
YIIHGVASSYGKTATFMPKPVIGDNGSGMHCHQSLWKDNINIFVNSDGSMSEICLYYIGGIIKYGKAINAFTNPSTNSYK
RLVPNFEAPTWLTYSYENRSSAIRIPYVPNTDLNAKRIEVRFPDPLANPYLCFSAQLMAGLYGIKNKIHPGPSIDKNLYT
LDKKELQSFISVANSLEESLYALDNNREFLLEGDVFTNDQIDTYIKLKSSEARDLHLHPHPIEFINYYSS
>Mature_470_residues
MFLSAEDTLNYIKKNTINFVDLRFTDSTSRWHHITRNAETIDTYVLSSGVNFDSSSIPGWQSVEKSDMILLPDVSTAFVD
PFTAQPTLVIICNVINPYDKSEYPKDPRYTAHKASQYMLSTGIADKCYFGPEVEFFVFDNATFYNGMYHSYFKIESSEHI
SQFQKREHINNSHCPKIKGGYMPTPPVDSLHDLRSEILTMLKEVGITPLIHHHEVAASQCEVGFQHDELIRSADNVQKCK
YIIHGVASSYGKTATFMPKPVIGDNGSGMHCHQSLWKDNINIFVNSDGSMSEICLYYIGGIIKYGKAINAFTNPSTNSYK
RLVPNFEAPTWLTYSYENRSSAIRIPYVPNTDLNAKRIEVRFPDPLANPYLCFSAQLMAGLYGIKNKIHPGPSIDKNLYT
LDKKELQSFISVANSLEESLYALDNNREFLLEGDVFTNDQIDTYIKLKSSEARDLHLHPHPIEFINYYSS

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=425, Percent_Identity=23.2941176470588, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1790301, Length=470, Percent_Identity=50.8510638297872, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI87081870, Length=207, Percent_Identity=28.9855072463768, Blast_Score=92, Evalue=9e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 53416; Mature: 53416

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLSAEDTLNYIKKNTINFVDLRFTDSTSRWHHITRNAETIDTYVLSSGVNFDSSSIPGW
CCCCHHHHHHHHHHCCCEEEEEEEECCCCHHHHHCCCCHHHHHHHEECCCCCCCCCCCCC
QSVEKSDMILLPDVSTAFVDPFTAQPTLVIICNVINPYDKSEYPKDPRYTAHKASQYMLS
CCCCCCCEEEECCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
TGIADKCYFGPEVEFFVFDNATFYNGMYHSYFKIESSEHISQFQKREHINNSHCPKIKGG
CCCCCCCCCCCCEEEEEECCCEEECCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCC
YMPTPPVDSLHDLRSEILTMLKEVGITPLIHHHEVAASQCEVGFQHDELIRSADNVQKCK
CCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YIIHGVASSYGKTATFMPKPVIGDNGSGMHCHQSLWKDNINIFVNSDGSMSEICLYYIGG
HHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHH
IIKYGKAINAFTNPSTNSYKRLVPNFEAPTWLTYSYENRSSAIRIPYVPNTDLNAKRIEV
HHHHCCHHCCCCCCCCCCHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEE
RFPDPLANPYLCFSAQLMAGLYGIKNKIHPGPSIDKNLYTLDKKELQSFISVANSLEESL
ECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHH
YALDNNREFLLEGDVFTNDQIDTYIKLKSSEARDLHLHPHPIEFINYYSS
HHCCCCCEEEEECCEECCCCCEEEEEEECCCCCEEECCCCHHHHHHCCCC
>Mature Secondary Structure
MFLSAEDTLNYIKKNTINFVDLRFTDSTSRWHHITRNAETIDTYVLSSGVNFDSSSIPGW
CCCCHHHHHHHHHHCCCEEEEEEEECCCCHHHHHCCCCHHHHHHHEECCCCCCCCCCCCC
QSVEKSDMILLPDVSTAFVDPFTAQPTLVIICNVINPYDKSEYPKDPRYTAHKASQYMLS
CCCCCCCEEEECCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
TGIADKCYFGPEVEFFVFDNATFYNGMYHSYFKIESSEHISQFQKREHINNSHCPKIKGG
CCCCCCCCCCCCEEEEEECCCEEECCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCC
YMPTPPVDSLHDLRSEILTMLKEVGITPLIHHHEVAASQCEVGFQHDELIRSADNVQKCK
CCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YIIHGVASSYGKTATFMPKPVIGDNGSGMHCHQSLWKDNINIFVNSDGSMSEICLYYIGG
HHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHH
IIKYGKAINAFTNPSTNSYKRLVPNFEAPTWLTYSYENRSSAIRIPYVPNTDLNAKRIEV
HHHHCCHHCCCCCCCCCCHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEE
RFPDPLANPYLCFSAQLMAGLYGIKNKIHPGPSIDKNLYTLDKKELQSFISVANSLEESL
ECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHH
YALDNNREFLLEGDVFTNDQIDTYIKLKSSEARDLHLHPHPIEFINYYSS
HHCCCCCEEEEECCEECCCCCEEEEEEECCCCCEEECCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275; 2859270; 2793830 [H]