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The map label for this gene is ispB [H]

Identifier: 58616777

GI number: 58616777

Start: 67363

End: 68340

Strand: Reverse

Name: ispB [H]

Synonym: ERGA_CDS_00500

Alternate gene names: 58616777

Gene position: 68340-67363 (Counterclockwise)

Preceding gene: 58616778

Following gene: 58616771

Centisome position: 4.56

GC content: 28.02

Gene sequence:

>978_bases
ATGGCAAATGATTTTTATACAACACTAACAGAATTAAAATCTCTCATACAACAAGATTTAGATGAAATGGAACATTTAAT
TTTATATCAAGATAACAAAAATATTACTTTAATTACAGAAATTATACAGCATCTAATCAAATCTGGAGGGAAAAGAATAC
GTCCTATAATTTTCTTTATTATATGCAAAATGTTAAATTACAATAAAAATGACAAAGTATACATTGCCGCAGCTATTGAG
TTCATACATAATGCAACACTGCTACATGATGATGTTATAGATGAGAGTGAATTAAGAAGAGGAGAAAAGACATCCAATTT
CCTTTGGGGAAATAAAGCAAGCATCCTAGTTGGAGATTTCTTACTTGCTGTATCTTTTCAATGGCTCATACAATGTAAAA
ATCTTACTATACTCTCCATATTATCTAAAACATCCAACACAATTATAATAGGAGAAGTCGAACAAATGGTCGCTAGTTAT
AACATAAACATATCACAAGAAAAATATATAGACATCATTTCTGCCAAAACAGCATCTCTTTTTGAAGCAACATGTGAATC
TGCTGCAAATCTAGCTGAAGTAAATAACGAAAAAATACATGCATTAAAACAATTTGGCTATAATTTGGGTATATCATTCC
AAATTTTAGATGATATTTTAGATTACACAGCATCAGAAACACAGTTTGGAAAAAAATTAGGCAACGATCTATTAACAGGT
AAAATTACTTTACCAATCATTATCGCATATCAAAATGCAAATCATCAAGAAAAAAAATTTTGGACTCAACTATCTACTAA
TCCAGATATAAATAAAGCAATGTTTTATATAAAATCCCATTCTGCAATTGAACAAGCACTCAACATAGCATCAACATATA
TAGACAAAGCAAAATCCAGTTTAAATATTTTTGCAAATTCAGATTACAAACAAATATTAGATAAATTACTTAACTCCATC
TTATATAGAAATTTTTAA

Upstream 100 bases:

>100_bases
TTATATACAACAGGTAACAAAAATTGAGAAAAACTTGATTAGAAAGGTTATCACACTGATACTAGGTATTAAAGTAATTT
AATAACAACATGGTTCTTAT

Downstream 100 bases:

>100_bases
CTAAATAATATCCTAATCAACACAACCTAAATATAAACCATAGAGTCTAGTTATGCATTATACAAATAATCACAAGTTAG
TGTACTAATAGGTTGATAAA

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MANDFYTTLTELKSLIQQDLDEMEHLILYQDNKNITLITEIIQHLIKSGGKRIRPIIFFIICKMLNYNKNDKVYIAAAIE
FIHNATLLHDDVIDESELRRGEKTSNFLWGNKASILVGDFLLAVSFQWLIQCKNLTILSILSKTSNTIIIGEVEQMVASY
NINISQEKYIDIISAKTASLFEATCESAANLAEVNNEKIHALKQFGYNLGISFQILDDILDYTASETQFGKKLGNDLLTG
KITLPIIIAYQNANHQEKKFWTQLSTNPDINKAMFYIKSHSAIEQALNIASTYIDKAKSSLNIFANSDYKQILDKLLNSI
LYRNF

Sequences:

>Translated_325_residues
MANDFYTTLTELKSLIQQDLDEMEHLILYQDNKNITLITEIIQHLIKSGGKRIRPIIFFIICKMLNYNKNDKVYIAAAIE
FIHNATLLHDDVIDESELRRGEKTSNFLWGNKASILVGDFLLAVSFQWLIQCKNLTILSILSKTSNTIIIGEVEQMVASY
NINISQEKYIDIISAKTASLFEATCESAANLAEVNNEKIHALKQFGYNLGISFQILDDILDYTASETQFGKKLGNDLLTG
KITLPIIIAYQNANHQEKKFWTQLSTNPDINKAMFYIKSHSAIEQALNIASTYIDKAKSSLNIFANSDYKQILDKLLNSI
LYRNF
>Mature_324_residues
ANDFYTTLTELKSLIQQDLDEMEHLILYQDNKNITLITEIIQHLIKSGGKRIRPIIFFIICKMLNYNKNDKVYIAAAIEF
IHNATLLHDDVIDESELRRGEKTSNFLWGNKASILVGDFLLAVSFQWLIQCKNLTILSILSKTSNTIIIGEVEQMVASYN
INISQEKYIDIISAKTASLFEATCESAANLAEVNNEKIHALKQFGYNLGISFQILDDILDYTASETQFGKKLGNDLLTGK
ITLPIIIAYQNANHQEKKFWTQLSTNPDINKAMFYIKSHSAIEQALNIASTYIDKAKSSLNIFANSDYKQILDKLLNSIL
YRNF

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=310, Percent_Identity=30, Blast_Score=155, Evalue=7e-38,
Organism=Escherichia coli, GI1789578, Length=320, Percent_Identity=35.3125, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1786623, Length=239, Percent_Identity=27.6150627615063, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=290, Percent_Identity=30.3448275862069, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6319475, Length=295, Percent_Identity=29.8305084745763, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325188, Length=228, Percent_Identity=27.1929824561404, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24651612, Length=282, Percent_Identity=26.9503546099291, Blast_Score=134, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36967; Mature: 36836

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDFYTTLTELKSLIQQDLDEMEHLILYQDNKNITLITEIIQHLIKSGGKRIRPIIFFI
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEHHHHHHHHHHHCCCCHHHHHHHHH
ICKMLNYNKNDKVYIAAAIEFIHNATLLHDDVIDESELRRGEKTSNFLWGNKASILVGDF
HHHHHCCCCCCCEEEHHHHHHHHCCHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHH
LLAVSFQWLIQCKNLTILSILSKTSNTIIIGEVEQMVASYNINISQEKYIDIISAKTASL
HHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FEATCESAANLAEVNNEKIHALKQFGYNLGISFQILDDILDYTASETQFGKKLGNDLLTG
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHEEC
KITLPIIIAYQNANHQEKKFWTQLSTNPDINKAMFYIKSHSAIEQALNIASTYIDKAKSS
EEEEEEEEEEECCCCHHHHHHHHCCCCCCHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCC
LNIFANSDYKQILDKLLNSILYRNF
EEEEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANDFYTTLTELKSLIQQDLDEMEHLILYQDNKNITLITEIIQHLIKSGGKRIRPIIFFI
CCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEHHHHHHHHHHHCCCCHHHHHHHHH
ICKMLNYNKNDKVYIAAAIEFIHNATLLHDDVIDESELRRGEKTSNFLWGNKASILVGDF
HHHHHCCCCCCCEEEHHHHHHHHCCHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHH
LLAVSFQWLIQCKNLTILSILSKTSNTIIIGEVEQMVASYNINISQEKYIDIISAKTASL
HHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FEATCESAANLAEVNNEKIHALKQFGYNLGISFQILDDILDYTASETQFGKKLGNDLLTG
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHEEC
KITLPIIIAYQNANHQEKKFWTQLSTNPDINKAMFYIKSHSAIEQALNIASTYIDKAKSS
EEEEEEEEEEECCCCHHHHHHHHCCCCCCHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCC
LNIFANSDYKQILDKLLNSILYRNF
EEEEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]