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The map label for this gene is citA [H]

Identifier: 56965801

GI number: 56965801

Start: 4216175

End: 4217266

Strand: Direct

Name: citA [H]

Synonym: ABC4043

Alternate gene names: 56965801

Gene position: 4216175-4217266 (Clockwise)

Preceding gene: 56965792

Following gene: 56965806

Centisome position: 97.96

GC content: 50.46

Gene sequence:

>1092_bases
ATGTTGTATGCAAAAGGGTTGGAGAATGTCGTCGTCGCAGAAACGACGATTAGTGCAATCGATGGCGAACAAGGAACACT
GCTTTACCGAGGGTATCAAGCAGAAGAACTTGCGAAATCTGCTGACTTTGAAGACGTTGCTTACTTACTGTTAAACGGAC
AAAGGCCAACGGAAGCAGAAAAAGACGATTTTTCCACTGCCTTGAAAACGATGCGTCCTTTGCCAGACGATTTGAAAAAG
CTGCTCGCTGCTTTGCCGAAAGACCTTAGTATGATGGACGCATTACGAACTTTGATTTCTAGCTACACTGGCAAGGAAGG
TTGGCCCCCGCAACAACAAGAAGCGATGCGCTTAGTCGCGATCGTTCCAACGATTGTGGCTGCGTGGGCCAATAGGAGCT
GCGGCAAAGACGCGATTGAACCAGACCCATCGCTCAACCACGTTGCTAATTACTTGTATATGCTTACTGGCGAAAAGCCA
CATGCCGCCCACATTCGTGCTTTAAGCGCTTATTTAATATTGACAGCGGAACACGGCATGAATGCGTCAACGTTTGCGGC
GCGGGTGATCGCCTCTACAGAAGCGGATCTTCGTTCGGCTATTTGTGGAGCGCTTGGCGCGTTAAAAGGAAGGCTGCACG
GCGGCGCGCCCACTGGTGTACTTGAGCTGTTCAGTGATATCCAAACGGCTGACACAAGCAGCGAAACGTTGCTGCGGGAA
AAGCTCGAACGCGGCGAGCGTTTAATGGGCTTTGGCCACCGTGTTTATAAAACGACCGACCCTCGCGCCAACGCATTGCG
TGAGGTGTTAAAAAGCCTCAATAACCAAGAGACATTTTTAAGCGAAGCAGTGGCGATTGAGCAACTAGCAACGCAGCTTT
TAGCGGAATACAAGCCAGGCCGCCAGCTTCATGCGAACGTTGAATATTACGCTGCTGCAATCATGGAAGCACTTCGCCTT
AAACCCGATTGGTTCACACCGACATTTTGTGTAAGCCGCGTCGTCGGATGGTGCAGCCATGTCATCGAACAAAGCCAAGA
CAACCGGATTATGAGGCCACAACAACAGTACTGCGGTCCAAAGCCGGACTAA

Upstream 100 bases:

>100_bases
ACTGATTAAATATCTTAATCAATAATGCAGAAATAATTTAATTTCATTTAGATTACCATGGCGCTATGATAAAGAAAATG
AATTTTGGGGGGATGGAAAT

Downstream 100 bases:

>100_bases
AGGAAACAACGACGTAGCAAGTGATTGCTAAAAAGTAGAAGACCCTGTACCAAGTGGCACAGGGTCTTAGCCGTTTCGGT
CATTCAAACAAACTAGGCAA

Product: citrate synthase I

Products: NA

Alternate protein names: Citrate synthase I [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MLYAKGLENVVVAETTISAIDGEQGTLLYRGYQAEELAKSADFEDVAYLLLNGQRPTEAEKDDFSTALKTMRPLPDDLKK
LLAALPKDLSMMDALRTLISSYTGKEGWPPQQQEAMRLVAIVPTIVAAWANRSCGKDAIEPDPSLNHVANYLYMLTGEKP
HAAHIRALSAYLILTAEHGMNASTFAARVIASTEADLRSAICGALGALKGRLHGGAPTGVLELFSDIQTADTSSETLLRE
KLERGERLMGFGHRVYKTTDPRANALREVLKSLNNQETFLSEAVAIEQLATQLLAEYKPGRQLHANVEYYAAAIMEALRL
KPDWFTPTFCVSRVVGWCSHVIEQSQDNRIMRPQQQYCGPKPD

Sequences:

>Translated_363_residues
MLYAKGLENVVVAETTISAIDGEQGTLLYRGYQAEELAKSADFEDVAYLLLNGQRPTEAEKDDFSTALKTMRPLPDDLKK
LLAALPKDLSMMDALRTLISSYTGKEGWPPQQQEAMRLVAIVPTIVAAWANRSCGKDAIEPDPSLNHVANYLYMLTGEKP
HAAHIRALSAYLILTAEHGMNASTFAARVIASTEADLRSAICGALGALKGRLHGGAPTGVLELFSDIQTADTSSETLLRE
KLERGERLMGFGHRVYKTTDPRANALREVLKSLNNQETFLSEAVAIEQLATQLLAEYKPGRQLHANVEYYAAAIMEALRL
KPDWFTPTFCVSRVVGWCSHVIEQSQDNRIMRPQQQYCGPKPD
>Mature_363_residues
MLYAKGLENVVVAETTISAIDGEQGTLLYRGYQAEELAKSADFEDVAYLLLNGQRPTEAEKDDFSTALKTMRPLPDDLKK
LLAALPKDLSMMDALRTLISSYTGKEGWPPQQQEAMRLVAIVPTIVAAWANRSCGKDAIEPDPSLNHVANYLYMLTGEKP
HAAHIRALSAYLILTAEHGMNASTFAARVIASTEADLRSAICGALGALKGRLHGGAPTGVLELFSDIQTADTSSETLLRE
KLERGERLMGFGHRVYKTTDPRANALREVLKSLNNQETFLSEAVAIEQLATQLLAEYKPGRQLHANVEYYAAAIMEALRL
KPDWFTPTFCVSRVVGWCSHVIEQSQDNRIMRPQQQYCGPKPD

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=380, Percent_Identity=24.4736842105263, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1786527, Length=362, Percent_Identity=35.6353591160221, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1786939, Length=375, Percent_Identity=32, Blast_Score=193, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17555174, Length=377, Percent_Identity=26.525198938992, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319850, Length=338, Percent_Identity=27.2189349112426, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324328, Length=337, Percent_Identity=26.1127596439169, Blast_Score=92, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24640124, Length=370, Percent_Identity=25.9459459459459, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24640126, Length=370, Percent_Identity=25.9459459459459, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21356863, Length=371, Percent_Identity=21.832884097035, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 40038; Mature: 40038

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYAKGLENVVVAETTISAIDGEQGTLLYRGYQAEELAKSADFEDVAYLLLNGQRPTEAE
CCCCCCCCCEEEHHHHHHHHCCCCCEEEEECCCHHHHHHHCCHHHHHHHHCCCCCCCCCC
KDDFSTALKTMRPLPDDLKKLLAALPKDLSMMDALRTLISSYTGKEGWPPQQQEAMRLVA
HHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
IVPTIVAAWANRSCGKDAIEPDPSLNHVANYLYMLTGEKPHAAHIRALSAYLILTAEHGM
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEECCCC
NASTFAARVIASTEADLRSAICGALGALKGRLHGGAPTGVLELFSDIQTADTSSETLLRE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
KLERGERLMGFGHRVYKTTDPRANALREVLKSLNNQETFLSEAVAIEQLATQLLAEYKPG
HHHHHHHHHHCCCHHEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
RQLHANVEYYAAAIMEALRLKPDWFTPTFCVSRVVGWCSHVIEQSQDNRIMRPQQQYCGP
CEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
KPD
CCC
>Mature Secondary Structure
MLYAKGLENVVVAETTISAIDGEQGTLLYRGYQAEELAKSADFEDVAYLLLNGQRPTEAE
CCCCCCCCCEEEHHHHHHHHCCCCCEEEEECCCHHHHHHHCCHHHHHHHHCCCCCCCCCC
KDDFSTALKTMRPLPDDLKKLLAALPKDLSMMDALRTLISSYTGKEGWPPQQQEAMRLVA
HHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
IVPTIVAAWANRSCGKDAIEPDPSLNHVANYLYMLTGEKPHAAHIRALSAYLILTAEHGM
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEECCCC
NASTFAARVIASTEADLRSAICGALGALKGRLHGGAPTGVLELFSDIQTADTSSETLLRE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
KLERGERLMGFGHRVYKTTDPRANALREVLKSLNNQETFLSEAVAIEQLATQLLAEYKPG
HHHHHHHHHHCCCHHEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
RQLHANVEYYAAAIMEALRLKPDWFTPTFCVSRVVGWCSHVIEQSQDNRIMRPQQQYCGP
CEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
KPD
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9579061; 9384377 [H]