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The map label for this gene is adhB [H]

Identifier: 56965175

GI number: 56965175

Start: 3538605

End: 3539744

Strand: Direct

Name: adhB [H]

Synonym: ABC3413

Alternate gene names: 56965175

Gene position: 3538605-3539744 (Clockwise)

Preceding gene: 56965174

Following gene: 56965176

Centisome position: 82.22

GC content: 46.67

Gene sequence:

>1140_bases
ATGAAAGCAGTTACCTTTCAAGGCAAAAAGCAGATGGAACCAAAGGAAGTACCTGCACCTTCCATTCAACAAGACACAGA
CATTATCGTAAGAATTACCGCAAGTGGTATTTGCGGATCGGATTTGCATTTGTATCACGGCGGCATCGTGCCTGAAACCG
ATTATGTAGTGGGCCATGAACCGATGGGCATCGTCGAGGAAGTCGGGCCAATGGTCAAGTCGTTAAAAAAGGGCGACCGC
GTCGTCATTCCTTTTAACGTCAGCTGCGGAGAATGTTTCTTTTGCCAACACCAAATGGAAAGCCAGTGCGACAACTCTAA
CGGAAACCCTCATTCGGATGCAGGCGGTTTATTTGGTTTTGCTGAGCAGTTTGGAAATTATCCAGGCGGGCAAGCCGAGT
ATTTGCGCGTGCCTTATGGCGATTTTACGTCGTTTAAGCTACCGGCCGAGAGTGAATTGGATGACGAGCAAGTTGTTTTA
TTATCGGATGTGATCCCTACTGCTTATTGGAGCGTCAAACATGCTGGGGTTTCGACAGGCGATACGGTTATTATTTTAGG
TTGTGGGCCGATTGGCTTAATGGCACAAAAATTTGCTTGGCTTCATGGCGCCAAACGTGTTATTGCTGTTGACCAAGTCG
CACACCGCCTTGAACATGGAAAACGGACCAACTTAGTGGAAACCTATAACTTCAGCAATACGCCTGACATAGGCAAACAG
CTCTATGAAGACACAGAGGGCGGCGCCGACGTTGTCATTGATTGCGTAGGCATGGATGGCACCGTTCCACCAGGAGAAAC
ATTTGGCTCTAGCGGGGACAACCAATTCGGGACAATCCAGCCAATTGTAACTGCCAGCGAAGCTGTACGGAAATTCGGGA
CGGTGCAAATTACCGGCGTATACGGCTCCGAAGCCAATCAATTCCCAATTAATGATTTTTTCACACGCAATGTTTCTTTA
AAAATGGGGCAAGCTCCTGTTGTCCATTTAATGCCCACCCTTTACACCATGATTGAAAGAGGAACGATTGACCCAACTGA
TATCATTACGCATCGAATGGGCATTGAAGATGCCCCAGACGCTTATCGGATATTTGACCAAAAAGAAGACGGCAACATTA
AAACGATTTTTAAATTGTAA

Upstream 100 bases:

>100_bases
TTGCAGCGGCGCTTTTATTATTAGATTGTCCAAGGAAGTTTTAATCTCCGTTAGAGAAGGAATAGCCCCTTACATACTAA
ACAAGGAGGGGCATTCACAG

Downstream 100 bases:

>100_bases
GGAATGTGCCCCATCACTCCTCGTTTGTTGAGTGATGGGGCACATCCAAGTGAGCAACGATTTTTGGTGGGCAGACCATG
ACTAGATACGCTGGGTTTAC

Product: glutathione-dependent formaldehyde dehydrogenase

Products: S-formylglutathione; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKAVTFQGKKQMEPKEVPAPSIQQDTDIIVRITASGICGSDLHLYHGGIVPETDYVVGHEPMGIVEEVGPMVKSLKKGDR
VVIPFNVSCGECFFCQHQMESQCDNSNGNPHSDAGGLFGFAEQFGNYPGGQAEYLRVPYGDFTSFKLPAESELDDEQVVL
LSDVIPTAYWSVKHAGVSTGDTVIILGCGPIGLMAQKFAWLHGAKRVIAVDQVAHRLEHGKRTNLVETYNFSNTPDIGKQ
LYEDTEGGADVVIDCVGMDGTVPPGETFGSSGDNQFGTIQPIVTASEAVRKFGTVQITGVYGSEANQFPINDFFTRNVSL
KMGQAPVVHLMPTLYTMIERGTIDPTDIITHRMGIEDAPDAYRIFDQKEDGNIKTIFKL

Sequences:

>Translated_379_residues
MKAVTFQGKKQMEPKEVPAPSIQQDTDIIVRITASGICGSDLHLYHGGIVPETDYVVGHEPMGIVEEVGPMVKSLKKGDR
VVIPFNVSCGECFFCQHQMESQCDNSNGNPHSDAGGLFGFAEQFGNYPGGQAEYLRVPYGDFTSFKLPAESELDDEQVVL
LSDVIPTAYWSVKHAGVSTGDTVIILGCGPIGLMAQKFAWLHGAKRVIAVDQVAHRLEHGKRTNLVETYNFSNTPDIGKQ
LYEDTEGGADVVIDCVGMDGTVPPGETFGSSGDNQFGTIQPIVTASEAVRKFGTVQITGVYGSEANQFPINDFFTRNVSL
KMGQAPVVHLMPTLYTMIERGTIDPTDIITHRMGIEDAPDAYRIFDQKEDGNIKTIFKL
>Mature_379_residues
MKAVTFQGKKQMEPKEVPAPSIQQDTDIIVRITASGICGSDLHLYHGGIVPETDYVVGHEPMGIVEEVGPMVKSLKKGDR
VVIPFNVSCGECFFCQHQMESQCDNSNGNPHSDAGGLFGFAEQFGNYPGGQAEYLRVPYGDFTSFKLPAESELDDEQVVL
LSDVIPTAYWSVKHAGVSTGDTVIILGCGPIGLMAQKFAWLHGAKRVIAVDQVAHRLEHGKRTNLVETYNFSNTPDIGKQ
LYEDTEGGADVVIDCVGMDGTVPPGETFGSSGDNQFGTIQPIVTASEAVRKFGTVQITGVYGSEANQFPINDFFTRNVSL
KMGQAPVVHLMPTLYTMIERGTIDPTDIITHRMGIEDAPDAYRIFDQKEDGNIKTIFKL

Specific function: Unknown

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501933, Length=350, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4501929, Length=352, Percent_Identity=28.125, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI34577061, Length=350, Percent_Identity=28.2857142857143, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI71565152, Length=395, Percent_Identity=23.5443037974684, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI71743840, Length=243, Percent_Identity=29.2181069958848, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI262073058, Length=243, Percent_Identity=29.2181069958848, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI71565154, Length=268, Percent_Identity=28.7313432835821, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI156523966, Length=390, Percent_Identity=23.3333333333333, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4501939, Length=376, Percent_Identity=23.1382978723404, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786825, Length=389, Percent_Identity=38.560411311054, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI87082125, Length=388, Percent_Identity=27.0618556701031, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787863, Length=385, Percent_Identity=25.7142857142857, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790045, Length=392, Percent_Identity=26.2755102040816, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788073, Length=384, Percent_Identity=26.3020833333333, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790718, Length=356, Percent_Identity=25.561797752809, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI226510992, Length=369, Percent_Identity=25.7452574525745, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788075, Length=370, Percent_Identity=26.7567567567568, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786552, Length=393, Percent_Identity=25.1908396946565, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788407, Length=245, Percent_Identity=28.1632653061224, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081918, Length=222, Percent_Identity=28.3783783783784, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562876, Length=372, Percent_Identity=26.3440860215054, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17562878, Length=378, Percent_Identity=24.8677248677249, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71997431, Length=392, Percent_Identity=26.530612244898, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25146526, Length=393, Percent_Identity=26.972010178117, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562584, Length=296, Percent_Identity=29.3918918918919, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562582, Length=374, Percent_Identity=26.2032085561497, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17565904, Length=361, Percent_Identity=26.8698060941828, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71988145, Length=211, Percent_Identity=31.7535545023697, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323099, Length=392, Percent_Identity=25.765306122449, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319258, Length=414, Percent_Identity=25.8454106280193, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319955, Length=373, Percent_Identity=27.3458445040214, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322619, Length=373, Percent_Identity=27.3458445040214, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323729, Length=352, Percent_Identity=25, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6320033, Length=278, Percent_Identity=28.0575539568345, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324486, Length=350, Percent_Identity=24.5714285714286, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323961, Length=351, Percent_Identity=24.7863247863248, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319621, Length=362, Percent_Identity=25.9668508287293, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319257, Length=405, Percent_Identity=25.9259259259259, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI17137530, Length=379, Percent_Identity=26.3852242744063, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17737897, Length=379, Percent_Identity=24.5382585751979, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17737895, Length=397, Percent_Identity=25.9445843828715, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI45550770, Length=345, Percent_Identity=23.1884057971014, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45551930, Length=345, Percent_Identity=23.1884057971014, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI221457811, Length=343, Percent_Identity=23.0320699708455, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.2.1.1

Molecular weight: Translated: 41287; Mature: 41287

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVTFQGKKQMEPKEVPAPSIQQDTDIIVRITASGICGSDLHLYHGGIVPETDYVVGHE
CCCEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCEEECCC
PMGIVEEVGPMVKSLKKGDRVVIPFNVSCGECFFCQHQMESQCDNSNGNPHSDAGGLFGF
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEHHHHHHHCCCCCCCCCCCCCCHHHH
AEQFGNYPGGQAEYLRVPYGDFTSFKLPAESELDDEQVVLLSDVIPTAYWSVKHAGVSTG
HHHHCCCCCCCCEEEEECCCCCCCEECCCCCCCCCCEEEEEHHHHHHHHHEEEECCCCCC
DTVIILGCGPIGLMAQKFAWLHGAKRVIAVDQVAHRLEHGKRTNLVETYNFSNTPDIGKQ
CEEEEEECCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHH
LYEDTEGGADVVIDCVGMDGTVPPGETFGSSGDNQFGTIQPIVTASEAVRKFGTVQITGV
HHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEE
YGSEANQFPINDFFTRNVSLKMGQAPVVHLMPTLYTMIERGTIDPTDIITHRMGIEDAPD
ECCCCCCCCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
AYRIFDQKEDGNIKTIFKL
HHHCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MKAVTFQGKKQMEPKEVPAPSIQQDTDIIVRITASGICGSDLHLYHGGIVPETDYVVGHE
CCCEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCEEECCC
PMGIVEEVGPMVKSLKKGDRVVIPFNVSCGECFFCQHQMESQCDNSNGNPHSDAGGLFGF
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEHHHHHHHCCCCCCCCCCCCCCHHHH
AEQFGNYPGGQAEYLRVPYGDFTSFKLPAESELDDEQVVLLSDVIPTAYWSVKHAGVSTG
HHHHCCCCCCCCEEEEECCCCCCCEECCCCCCCCCCEEEEEHHHHHHHHHEEEECCCCCC
DTVIILGCGPIGLMAQKFAWLHGAKRVIAVDQVAHRLEHGKRTNLVETYNFSNTPDIGKQ
CEEEEEECCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHH
LYEDTEGGADVVIDCVGMDGTVPPGETFGSSGDNQFGTIQPIVTASEAVRKFGTVQITGV
HHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEE
YGSEANQFPINDFFTRNVSLKMGQAPVVHLMPTLYTMIERGTIDPTDIITHRMGIEDAPD
ECCCCCCCCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
AYRIFDQKEDGNIKTIFKL
HHHCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formaldehyde; glutathione; NAD+

Specific reaction: formaldehyde + glutathione + NAD+ = S-formylglutathione + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9141695; 9384377 [H]