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The map label for this gene is glpF [H]

Identifier: 56965145

GI number: 56965145

Start: 3510304

End: 3511008

Strand: Direct

Name: glpF [H]

Synonym: ABC3383

Alternate gene names: 56965145

Gene position: 3510304-3511008 (Clockwise)

Preceding gene: 56965142

Following gene: 56965146

Centisome position: 81.56

GC content: 50.35

Gene sequence:

>705_bases
ATGGCAGAAATCATGGCAGAGCTTATCGGCACTATGGTTCTTATCATTTTTGGTGCTGGCGTTGTCGCCGGTGTGACGCT
AAAACAATCAAAAGGAGAAGGGGCAGGCTGGATTGCGATTTCGGTAGGCTGGGGCTTTGCAGTAGCGCTTGGCGTTTATG
TGGCCGGCACTGTGAGTGACGCCCATTTGAACCCTGCTGTCACGCTTGGATTTGCTGCCATCGGACAGTTTCCTTGGGCG
CAAGTACCTGGCTATATAATCGGACAGTTAGCCGGAGCCTTTCTTGGTGCCGTTATTGTCTTTCTTCATTATTATCCACA
CTGGAAGGCAACGGAAGATGCAGGAGTTAAACTGGGCGTATTTGCAACTGGTCCGGCGATTCGCCACCTCCCAGCCAATT
TCTTAAGCGAATTAATTGGCACTGCTGTATTGGTATTCGGGTTATTGGCTATTGGCGCCAACACATTCAGCGAAGGATTG
AATCCAATTGTCGTTGGCTTGCTCATCATTGCGATTGGCCTTTCCCTCGGGGGCACGACTGGCTATGCCATTAACCCAGC
ACGTGACCTAGGGCCACGCCTTGCCCACGCTTTATTGCCAATTAACAAAAAAGGGCCATCCGATTGGGCTTATGCATGGG
TGCCCATTCTCGGGCCATTTGTTGGCGGTATTGTTGGCGCTGTTCTTTATACCCTTATTTTCTAA

Upstream 100 bases:

>100_bases
CTGCGCGACCCTGTGATTAAGGCTGCTCTTTTTTATGCAAATGCAGTTTCGTTTCTTCCAATTTGGCTTTCGGCTTGACA
CTATGAAGGAGGTACGAAAA

Downstream 100 bases:

>100_bases
ACAAAGGAGACGATATTGATGAAAAACCCATACATTTTATCCATCGATCAAGGAACAACTAGTTCACGCGCGATTTTATT
TGACAGCCAAGGAGAAATCA

Product: glycerol uptake facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MAEIMAELIGTMVLIIFGAGVVAGVTLKQSKGEGAGWIAISVGWGFAVALGVYVAGTVSDAHLNPAVTLGFAAIGQFPWA
QVPGYIIGQLAGAFLGAVIVFLHYYPHWKATEDAGVKLGVFATGPAIRHLPANFLSELIGTAVLVFGLLAIGANTFSEGL
NPIVVGLLIIAIGLSLGGTTGYAINPARDLGPRLAHALLPINKKGPSDWAYAWVPILGPFVGGIVGAVLYTLIF

Sequences:

>Translated_234_residues
MAEIMAELIGTMVLIIFGAGVVAGVTLKQSKGEGAGWIAISVGWGFAVALGVYVAGTVSDAHLNPAVTLGFAAIGQFPWA
QVPGYIIGQLAGAFLGAVIVFLHYYPHWKATEDAGVKLGVFATGPAIRHLPANFLSELIGTAVLVFGLLAIGANTFSEGL
NPIVVGLLIIAIGLSLGGTTGYAINPARDLGPRLAHALLPINKKGPSDWAYAWVPILGPFVGGIVGAVLYTLIF
>Mature_233_residues
AEIMAELIGTMVLIIFGAGVVAGVTLKQSKGEGAGWIAISVGWGFAVALGVYVAGTVSDAHLNPAVTLGFAAIGQFPWAQ
VPGYIIGQLAGAFLGAVIVFLHYYPHWKATEDAGVKLGVFATGPAIRHLPANFLSELIGTAVLVFGLLAIGANTFSEGLN
PIVVGLLIIAIGLSLGGTTGYAINPARDLGPRLAHALLPINKKGPSDWAYAWVPILGPFVGGIVGAVLYTLIF

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=249, Percent_Identity=41.3654618473896, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI157266307, Length=248, Percent_Identity=40.3225806451613, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI4502187, Length=247, Percent_Identity=36.0323886639676, Blast_Score=131, Evalue=4e-31,
Organism=Homo sapiens, GI22538420, Length=247, Percent_Identity=36.0323886639676, Blast_Score=131, Evalue=6e-31,
Organism=Homo sapiens, GI310133356, Length=169, Percent_Identity=35.5029585798817, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI310114181, Length=169, Percent_Identity=35.5029585798817, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1790362, Length=246, Percent_Identity=39.0243902439024, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1787101, Length=193, Percent_Identity=31.0880829015544, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17531429, Length=244, Percent_Identity=36.4754098360656, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17531431, Length=244, Percent_Identity=36.4754098360656, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71994009, Length=244, Percent_Identity=31.9672131147541, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17533613, Length=242, Percent_Identity=31.8181818181818, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32564052, Length=242, Percent_Identity=31.8181818181818, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17544068, Length=240, Percent_Identity=35.8333333333333, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71992966, Length=245, Percent_Identity=31.4285714285714, Blast_Score=106, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71992961, Length=239, Percent_Identity=29.2887029288703, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17558372, Length=236, Percent_Identity=26.6949152542373, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71993722, Length=233, Percent_Identity=26.6094420600858, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=206, Percent_Identity=39.3203883495146, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322985, Length=287, Percent_Identity=32.404181184669, Blast_Score=115, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24652747, Length=235, Percent_Identity=32.3404255319149, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI45551084, Length=235, Percent_Identity=32.3404255319149, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24762342, Length=199, Percent_Identity=30.6532663316583, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24058; Mature: 23926

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIMAELIGTMVLIIFGAGVVAGVTLKQSKGEGAGWIAISVGWGFAVALGVYVAGTVSD
CHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCEEEEEECHHHHHHHHHHHHCCCCC
AHLNPAVTLGFAAIGQFPWAQVPGYIIGQLAGAFLGAVIVFLHYYPHWKATEDAGVKLGV
CCCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
FATGPAIRHLPANFLSELIGTAVLVFGLLAIGANTFSEGLNPIVVGLLIIAIGLSLGGTT
EECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRLAHALLPINKKGPSDWAYAWVPILGPFVGGIVGAVLYTLIF
CCEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AEIMAELIGTMVLIIFGAGVVAGVTLKQSKGEGAGWIAISVGWGFAVALGVYVAGTVSD
HHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCEEEEEECHHHHHHHHHHHHCCCCC
AHLNPAVTLGFAAIGQFPWAQVPGYIIGQLAGAFLGAVIVFLHYYPHWKATEDAGVKLGV
CCCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
FATGPAIRHLPANFLSELIGTAVLVFGLLAIGANTFSEGLNPIVVGLLIIAIGLSLGGTT
EECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRLAHALLPINKKGPSDWAYAWVPILGPFVGGIVGAVLYTLIF
CCEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]