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The map label for this gene is purU [H]

Identifier: 56964545

GI number: 56964545

Start: 2915245

End: 2916108

Strand: Direct

Name: purU [H]

Synonym: ABC2781

Alternate gene names: 56964545

Gene position: 2915245-2916108 (Clockwise)

Preceding gene: 56964543

Following gene: 56964556

Centisome position: 67.74

GC content: 43.87

Gene sequence:

>864_bases
ATGGCTATTGAACAACAACGAGCGCGTTTGCTCGTTTCCTGCCAAGACCGTCCAGGCGTGGTCGCCAACATATCATCTTT
TCTATACAACCATGAGGCGAATATTGTCCAATCTGACCAATATTCGACTGACCCTGAGGGCGGCATGTTTTTCATGAGAA
TTGAATTTGCATGGCAGGAAGAAAAAACAGCGTTTTCCGAAATTAAACAAGCTTTTTCTGTCCTTGCAGACGCTGAACAT
TACCAATGGCGGATGGAGCAAGCTTCGCGAAAAAAGCGGATGGCGATTTTCGTCTCAAAAGAAAATCATTGCTTGTCAGA
GCTGTTATGGAAGTGGCGAGCTGGCGAACTTTATGCGGAAATCCCACTTGTCATTAGCAATCATCCTGACAATAAAGAAG
AAGTGGAAGCTTACGGCATTCCCTTTTTCCATATACCGTCTACAAAGGCTAATCGCCGAGAAGCAGAGGACAAAGCAATT
GAACTTCTCCATGAACACAATATTGAACTTATCGTTCTTGCCCGTTACATGCAAATTTTATCGCCAACATTTGTTAGCAC
GTTCCCTCAACAAATCATTAACATCCATCATTCGTTTTTGCCTGCCTTTATCGGCGCCAACCCTTATGCAAAAGCATTTG
AACGAGGCGTAAAACTGATTGGCGCTACCGCCCATTACGTTACAGACGATTTGGATGAGGGTCCGATTATTGAACAAGAT
GTGTTACGAGTGAACCACCGCCATACAACCGCCGACTTGCGAATCGCTGGCCGTCAAATCGAGCGGATTGCATTGGCCCG
AGCTGTAAACTGGCATTTAAATGACCAATTGATTGTTTATAACAATAAAACAATTGTCTTTTAA

Upstream 100 bases:

>100_bases
TGATTCAATAAATAAGTCAGCCTTTCCTTCTACTTTGCGCTTTTAAAAAACAAAGTAGACAGGAGGGGATGTGTAAGCTC
AAAATTGGAGGAACAACAAA

Downstream 100 bases:

>100_bases
CGTTTATAAAAGCAGAGTGGCCATCATGCCAGCTCTGCTTTTACTGCTCATACATCATTTTTCTCGTCATACCACCATCA
ATAACAATCGTTTCGCCATT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MAIEQQRARLLVSCQDRPGVVANISSFLYNHEANIVQSDQYSTDPEGGMFFMRIEFAWQEEKTAFSEIKQAFSVLADAEH
YQWRMEQASRKKRMAIFVSKENHCLSELLWKWRAGELYAEIPLVISNHPDNKEEVEAYGIPFFHIPSTKANRREAEDKAI
ELLHEHNIELIVLARYMQILSPTFVSTFPQQIINIHHSFLPAFIGANPYAKAFERGVKLIGATAHYVTDDLDEGPIIEQD
VLRVNHRHTTADLRIAGRQIERIALARAVNWHLNDQLIVYNNKTIVF

Sequences:

>Translated_287_residues
MAIEQQRARLLVSCQDRPGVVANISSFLYNHEANIVQSDQYSTDPEGGMFFMRIEFAWQEEKTAFSEIKQAFSVLADAEH
YQWRMEQASRKKRMAIFVSKENHCLSELLWKWRAGELYAEIPLVISNHPDNKEEVEAYGIPFFHIPSTKANRREAEDKAI
ELLHEHNIELIVLARYMQILSPTFVSTFPQQIINIHHSFLPAFIGANPYAKAFERGVKLIGATAHYVTDDLDEGPIIEQD
VLRVNHRHTTADLRIAGRQIERIALARAVNWHLNDQLIVYNNKTIVF
>Mature_286_residues
AIEQQRARLLVSCQDRPGVVANISSFLYNHEANIVQSDQYSTDPEGGMFFMRIEFAWQEEKTAFSEIKQAFSVLADAEHY
QWRMEQASRKKRMAIFVSKENHCLSELLWKWRAGELYAEIPLVISNHPDNKEEVEAYGIPFFHIPSTKANRREAEDKAIE
LLHEHNIELIVLARYMQILSPTFVSTFPQQIINIHHSFLPAFIGANPYAKAFERGVKLIGATAHYVTDDLDEGPIIEQDV
LRVNHRHTTADLRIAGRQIERIALARAVNWHLNDQLIVYNNKTIVF

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=180, Percent_Identity=36.1111111111111, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI209869995, Length=180, Percent_Identity=36.1111111111111, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI209869993, Length=180, Percent_Identity=36.1111111111111, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1787483, Length=283, Percent_Identity=43.8162544169611, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1788846, Length=194, Percent_Identity=28.3505154639175, Blast_Score=85, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=192, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24582400, Length=186, Percent_Identity=31.1827956989247, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33128; Mature: 32997

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEQQRARLLVSCQDRPGVVANISSFLYNHEANIVQSDQYSTDPEGGMFFMRIEFAWQE
CCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEEEEEEECH
EKTAFSEIKQAFSVLADAEHYQWRMEQASRKKRMAIFVSKENHCLSELLWKWRAGELYAE
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCCEEEE
IPLVISNHPDNKEEVEAYGIPFFHIPSTKANRREAEDKAIELLHEHNIELIVLARYMQIL
CCEEEECCCCCHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
SPTFVSTFPQQIINIHHSFLPAFIGANPYAKAFERGVKLIGATAHYVTDDLDEGPIIEQD
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECHHHHHCCCCCCCCCCHHH
VLRVNHRHTTADLRIAGRQIERIALARAVNWHLNDQLIVYNNKTIVF
HHHHCCCCCCHHEEEHHHHHHHHHHHHHHEECCCCEEEEECCCEEEC
>Mature Secondary Structure 
AIEQQRARLLVSCQDRPGVVANISSFLYNHEANIVQSDQYSTDPEGGMFFMRIEFAWQE
CCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEEEEEEECH
EKTAFSEIKQAFSVLADAEHYQWRMEQASRKKRMAIFVSKENHCLSELLWKWRAGELYAE
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCCEEEE
IPLVISNHPDNKEEVEAYGIPFFHIPSTKANRREAEDKAIELLHEHNIELIVLARYMQIL
CCEEEECCCCCHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
SPTFVSTFPQQIINIHHSFLPAFIGANPYAKAFERGVKLIGATAHYVTDDLDEGPIIEQD
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECHHHHHCCCCCCCCCCHHH
VLRVNHRHTTADLRIAGRQIERIALARAVNWHLNDQLIVYNNKTIVF
HHHHCCCCCCHHEEEHHHHHHHHHHHHHHEECCCCEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]