Click here to switch to the map view.

The map label for this gene is asnB [H]

Identifier: 56964345

GI number: 56964345

Start: 2702765

End: 2704669

Strand: Direct

Name: asnB [H]

Synonym: ABC2580

Alternate gene names: 56964345

Gene position: 2702765-2704669 (Clockwise)

Preceding gene: 56964330

Following gene: 56964360

Centisome position: 62.8

GC content: 44.88

Gene sequence:

>1905_bases
ATGTGTGGTTTTGTCGGCGATCTGCTTATTCGAAATGGCAGCCATTTGCCTGATCATTTGTGGATTCAAACGCTAGAAGC
AATTCATCACCGTGGCCCTGACAGCACCGGTACTTATAAAGATGAAACAGTCCGTTTTGGATTTAAACGCTTAAGCATTA
TTGATTTAGAGCACGGCGACCAGCCCCTTGCATATAGCAAAGGGCGTTACCATATTGTTTTTAATGGCGAAATATATAAT
TACATCGAGCTGCGTTCTCAATTAATTCAACGCGGTTACACATTTCGAACCGATTCTGATACCGAAGTACTCCTCGCTCT
TTATGCAGCAGATGGCAAAAATGCGGTTAAACAATTACGGGGAATGTATGCGTTTGCGATTTGGGACAAGCACAAAAAGG
AATTGTTTTGTGCGCGTGACCGTTTTGGCATTAAGCCGTTTTACTATCTGAAAACAGACGAACGTTTTTTCTTTGCCTCT
GAACAAAAATCAATCATGCCATTGCTTGAGCAAATCTCGATTGAGCAAGAAGCGCTCCAACATTACGCCACCTTTCAGTT
TGTTCCCGAACCTAACACGCTGACTAAAGGCATTCATAAGCTACTGCCAGGACATACATTAACGGTCAATGCAGACTTTT
GTGTGCAAATTGAAGCATATGCTGAACGGACTTTTCAGCCTAGATCGGGGCCGACGGTCACAGAGCTTGCCAACCAAACA
CGAAAATTGTTAACAGACTCTGTCGAAAAACATATGCGCTCTGATGTCCCAGTTGGGGCATTTTTATCAAGCGGCATTGA
TTCGACTTCGATTGCTGCCTTGGCGCAACAAGTGCACCCTAACCTATTGACTTTTACGGTTGGTTTCGAGACAGAGGGCT
ATAGCGAAATTGCCGTCGCAAAAGAAACAGCAAAAGCATTAGGCGTCAAAAATATTCATAAAGTCATATCTGCTGATGAA
TTTATTCAAGAACTGCCTAACATCGTGTGGCATATGGACGAGCCTGTTGCAGACCCAGCAGCAGTTCCCCTTTATTTCGT
CGCCCAAGAAGCAAGCAAACATGTAAAGGTGGTTTTGTCAGGAGAAGGCGCTGACGAATTGTTCGGAGGGTACAACATTT
ACCGGGAACCTCTTGACCTTGCCTGGTTCAAAAAGTTGCCGCCTACGTTTCACGCTGGCTTAAAGCGGGTTGTTGCCCAT
ATGCCGTCTAAGCTGAAGGGACGAAACTACATTATACGGGGAACGACTCCCCTCAACGAGCGGTACATTGGCAATGCCTT
TATTTTTTCTGAAAAAGAAAAAACACAGTTGTTCCGCGACTATGATCGAACCAAACCTTACACCAATGTAACAGCGCCAC
TGTACGAACAGGCAAAAGGCATGGATGAATCGACAATTATGCAATTTGTCGATATGCATACGTGGCTTCCTGGCGACATC
CTTGTGAAGGCTGACCGAATGACAATGGCACATTCGATTGAATTAAGAGTCCCTTTTCTCGATAAAGATGTATTTGCATT
TGCGAGAATGCTTGGCCAGGATATGAAACTTTCCCATAAGACAACTAAATTTGTGCTCCGGCAAGCCATGAAGGACCTTA
TTCCGGCAAATATTGTGCCCCGTAAAAAATTAGGTTTTCCAGTGCCGATTCGCGTATGGCTCAAAAACGAACTGTACGAT
TGGGCTAAGCAGCTTATCGAGTCAAGCAATACAGAAGACGTGTTTCATAAGCCTTTTTTGCTGCAAATGCTGCAAGCACA
CCGGGACGGCCACGGCGATTATAGCCGCCGTTTATGGACAGTCCTTACGTATATGATGTGGCATGACATCCACGTTGCCA
AGCGACTGATTAGCACACATCAGCAGAAAGACGCTGAGCGCTGTAAAACGCTGGCCCATTTTTAA

Upstream 100 bases:

>100_bases
TGTCCTACGTTATTAGTCGACTGTTACCCTTTTAGCAAAAAATTGACTTTTATATTTAATTGGAGTAAGATTAGTTCACT
AAAAAACTGGAGGAAACATT

Downstream 100 bases:

>100_bases
ACATTGCTAGGAGGGCAGTTAAACATCGGTTTAATTGCCCTCTTCGTATCCATATATGCCTAACTAGCAAGTAGTTTATT
GTTTCTGGACTTGATGTTCA

Product: asparagine synthetase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MCGFVGDLLIRNGSHLPDHLWIQTLEAIHHRGPDSTGTYKDETVRFGFKRLSIIDLEHGDQPLAYSKGRYHIVFNGEIYN
YIELRSQLIQRGYTFRTDSDTEVLLALYAADGKNAVKQLRGMYAFAIWDKHKKELFCARDRFGIKPFYYLKTDERFFFAS
EQKSIMPLLEQISIEQEALQHYATFQFVPEPNTLTKGIHKLLPGHTLTVNADFCVQIEAYAERTFQPRSGPTVTELANQT
RKLLTDSVEKHMRSDVPVGAFLSSGIDSTSIAALAQQVHPNLLTFTVGFETEGYSEIAVAKETAKALGVKNIHKVISADE
FIQELPNIVWHMDEPVADPAAVPLYFVAQEASKHVKVVLSGEGADELFGGYNIYREPLDLAWFKKLPPTFHAGLKRVVAH
MPSKLKGRNYIIRGTTPLNERYIGNAFIFSEKEKTQLFRDYDRTKPYTNVTAPLYEQAKGMDESTIMQFVDMHTWLPGDI
LVKADRMTMAHSIELRVPFLDKDVFAFARMLGQDMKLSHKTTKFVLRQAMKDLIPANIVPRKKLGFPVPIRVWLKNELYD
WAKQLIESSNTEDVFHKPFLLQMLQAHRDGHGDYSRRLWTVLTYMMWHDIHVAKRLISTHQQKDAERCKTLAHF

Sequences:

>Translated_634_residues
MCGFVGDLLIRNGSHLPDHLWIQTLEAIHHRGPDSTGTYKDETVRFGFKRLSIIDLEHGDQPLAYSKGRYHIVFNGEIYN
YIELRSQLIQRGYTFRTDSDTEVLLALYAADGKNAVKQLRGMYAFAIWDKHKKELFCARDRFGIKPFYYLKTDERFFFAS
EQKSIMPLLEQISIEQEALQHYATFQFVPEPNTLTKGIHKLLPGHTLTVNADFCVQIEAYAERTFQPRSGPTVTELANQT
RKLLTDSVEKHMRSDVPVGAFLSSGIDSTSIAALAQQVHPNLLTFTVGFETEGYSEIAVAKETAKALGVKNIHKVISADE
FIQELPNIVWHMDEPVADPAAVPLYFVAQEASKHVKVVLSGEGADELFGGYNIYREPLDLAWFKKLPPTFHAGLKRVVAH
MPSKLKGRNYIIRGTTPLNERYIGNAFIFSEKEKTQLFRDYDRTKPYTNVTAPLYEQAKGMDESTIMQFVDMHTWLPGDI
LVKADRMTMAHSIELRVPFLDKDVFAFARMLGQDMKLSHKTTKFVLRQAMKDLIPANIVPRKKLGFPVPIRVWLKNELYD
WAKQLIESSNTEDVFHKPFLLQMLQAHRDGHGDYSRRLWTVLTYMMWHDIHVAKRLISTHQQKDAERCKTLAHF
>Mature_634_residues
MCGFVGDLLIRNGSHLPDHLWIQTLEAIHHRGPDSTGTYKDETVRFGFKRLSIIDLEHGDQPLAYSKGRYHIVFNGEIYN
YIELRSQLIQRGYTFRTDSDTEVLLALYAADGKNAVKQLRGMYAFAIWDKHKKELFCARDRFGIKPFYYLKTDERFFFAS
EQKSIMPLLEQISIEQEALQHYATFQFVPEPNTLTKGIHKLLPGHTLTVNADFCVQIEAYAERTFQPRSGPTVTELANQT
RKLLTDSVEKHMRSDVPVGAFLSSGIDSTSIAALAQQVHPNLLTFTVGFETEGYSEIAVAKETAKALGVKNIHKVISADE
FIQELPNIVWHMDEPVADPAAVPLYFVAQEASKHVKVVLSGEGADELFGGYNIYREPLDLAWFKKLPPTFHAGLKRVVAH
MPSKLKGRNYIIRGTTPLNERYIGNAFIFSEKEKTQLFRDYDRTKPYTNVTAPLYEQAKGMDESTIMQFVDMHTWLPGDI
LVKADRMTMAHSIELRVPFLDKDVFAFARMLGQDMKLSHKTTKFVLRQAMKDLIPANIVPRKKLGFPVPIRVWLKNELYD
WAKQLIESSNTEDVFHKPFLLQMLQAHRDGHGDYSRRLWTVLTYMMWHDIHVAKRLISTHQQKDAERCKTLAHF

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=384, Percent_Identity=28.6458333333333, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI168229252, Length=384, Percent_Identity=28.6458333333333, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI168229250, Length=384, Percent_Identity=28.6458333333333, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI296010848, Length=372, Percent_Identity=28.2258064516129, Blast_Score=130, Evalue=5e-30,
Organism=Homo sapiens, GI296010852, Length=300, Percent_Identity=26.6666666666667, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI296010850, Length=300, Percent_Identity=26.6666666666667, Blast_Score=99, Evalue=2e-20,
Organism=Escherichia coli, GI1786889, Length=393, Percent_Identity=30.7888040712468, Blast_Score=168, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI71993933, Length=359, Percent_Identity=30.9192200557103, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25147557, Length=359, Percent_Identity=30.9192200557103, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17560178, Length=385, Percent_Identity=28.8311688311688, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI25147560, Length=294, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321563, Length=388, Percent_Identity=27.0618556701031, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325403, Length=394, Percent_Identity=26.6497461928934, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI45553209, Length=457, Percent_Identity=29.3216630196937, Blast_Score=163, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72744; Mature: 72744

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGFVGDLLIRNGSHLPDHLWIQTLEAIHHRGPDSTGTYKDETVRFGFKRLSIIDLEHGD
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEEEEECCCC
QPLAYSKGRYHIVFNGEIYNYIELRSQLIQRGYTFRTDSDTEVLLALYAADGKNAVKQLR
CCCEECCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCHHHHHHHH
GMYAFAIWDKHKKELFCARDRFGIKPFYYLKTDERFFFASEQKSIMPLLEQISIEQEALQ
HHEEEEEHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
HYATFQFVPEPNTLTKGIHKLLPGHTLTVNADFCVQIEAYAERTFQPRSGPTVTELANQT
HHHHEEEECCCCHHHHHHHHHCCCCEEEECCCEEEEEEHHHHHCCCCCCCCHHHHHHHHH
RKLLTDSVEKHMRSDVPVGAFLSSGIDSTSIAALAQQVHPNLLTFTVGFETEGYSEIAVA
HHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHH
KETAKALGVKNIHKVISADEFIQELPNIVWHMDEPVADPAAVPLYFVAQEASKHVKVVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHEEEHHCCCCEEEEEE
GEGADELFGGYNIYREPLDLAWFKKLPPTFHAGLKRVVAHMPSKLKGRNYIIRGTTPLNE
CCCHHHHHCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCHHCCCCEEEEECCCCCCC
RYIGNAFIFSEKEKTQLFRDYDRTKPYTNVTAPLYEQAKGMDESTIMQFVDMHTWLPGDI
CCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCE
LVKADRMTMAHSIELRVPFLDKDVFAFARMLGQDMKLSHKTTKFVLRQAMKDLIPANIVP
EEEECCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
RKKLGFPVPIRVWLKNELYDWAKQLIESSNTEDVFHKPFLLQMLQAHRDGHGDYSRRLWT
HHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHCCCCCHHHHHHHH
VLTYMMWHDIHVAKRLISTHQQKDAERCKTLAHF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGFVGDLLIRNGSHLPDHLWIQTLEAIHHRGPDSTGTYKDETVRFGFKRLSIIDLEHGD
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEEEEECCCC
QPLAYSKGRYHIVFNGEIYNYIELRSQLIQRGYTFRTDSDTEVLLALYAADGKNAVKQLR
CCCEECCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCHHHHHHHH
GMYAFAIWDKHKKELFCARDRFGIKPFYYLKTDERFFFASEQKSIMPLLEQISIEQEALQ
HHEEEEEHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
HYATFQFVPEPNTLTKGIHKLLPGHTLTVNADFCVQIEAYAERTFQPRSGPTVTELANQT
HHHHEEEECCCCHHHHHHHHHCCCCEEEECCCEEEEEEHHHHHCCCCCCCCHHHHHHHHH
RKLLTDSVEKHMRSDVPVGAFLSSGIDSTSIAALAQQVHPNLLTFTVGFETEGYSEIAVA
HHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHH
KETAKALGVKNIHKVISADEFIQELPNIVWHMDEPVADPAAVPLYFVAQEASKHVKVVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHEEEHHCCCCEEEEEE
GEGADELFGGYNIYREPLDLAWFKKLPPTFHAGLKRVVAHMPSKLKGRNYIIRGTTPLNE
CCCHHHHHCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCHHCCCCEEEEECCCCCCC
RYIGNAFIFSEKEKTQLFRDYDRTKPYTNVTAPLYEQAKGMDESTIMQFVDMHTWLPGDI
CCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCE
LVKADRMTMAHSIELRVPFLDKDVFAFARMLGQDMKLSHKTTKFVLRQAMKDLIPANIVP
EEEECCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
RKKLGFPVPIRVWLKNELYDWAKQLIESSNTEDVFHKPFLLQMLQAHRDGHGDYSRRLWT
HHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHCCCCCHHHHHHHH
VLTYMMWHDIHVAKRLISTHQQKDAERCKTLAHF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]