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The map label for this gene is polA [C]

Identifier: 56963870

GI number: 56963870

Start: 2224717

End: 2225625

Strand: Direct

Name: polA [C]

Synonym: ABC2105

Alternate gene names: 56963870

Gene position: 2224717-2225625 (Clockwise)

Preceding gene: 56963869

Following gene: 56963872

Centisome position: 51.69

GC content: 45.43

Gene sequence:

>909_bases
TTGGCCAATGAACAGGCTCTGCTATTAGTCGACGGCTTTAACTTATTGAGCCGCGGGTATTTTGCGACGAGCTATAACCG
CCCGATTGAAAAGTTAGAACGAAACGACGAAGGCTTATATGTGAACGGCCTTCGTGTGTTTATCCAAAAACTATTTAAGC
TAGTTACCGATTATTCGATTTCCCATTGCATCGTCACTTGGGATGTGCCTCGGGAGGAAGCCTTGCGAAGAAAAACGTTT
CCCGACTACAAATTGACGCGAAATGAGCTTCCGGAACCATTAATCCAACAATATCAACAATGTGTGAAAACACTTTCCTA
CATCGGGGTTGAGCAATTAAAAGTGCCGCCATATGAGGCAGATGACATTATCGGTGCTATTTCGGCCAAATGGGAAGCTG
AAACGAGCGGTCCTTGCTATATTTACAGCAACGATAAAGATTTGTTTCAATTGCTGAGTTCCCGCACCACTCAAATTGTT
GCTAAAAAGCAAACAGAAGTGTTATTCGATGAAGCGTCGTTCAAAGCCAGTTACGGCCTCTCCCCTACCCAGTGGGTGGA
TGTAAAGGCGCTCGTCGGCGATACAAGTGATAATATTCCAGGCTGTCCCGGAATAGGCGAAAAAACCGCCCTGCCTCTCA
TCGCTGCGTACGGGTCGATTGAATCGCTATATCAACAAATCGACACAATCCAGCCGCCATTAAAACGGGCACAGAAAAAA
CTACTAGCTGGACAAGCGTCTACTCAGCTTTCAAAAAAGCTTTCTGCCATTATCTGCGACATTCCCGAACTCCGCCACTA
TTCTATCAATGACGCTGTTCTTTCTTTGAATCGGGGCGAAGTTGACAAGGAACTTTCACGTCTTGCTATTCGCGTTAAAT
TGGCGGACGAGTTGGCTTTTATTTCTTAA

Upstream 100 bases:

>100_bases
GGAACGGCTTTGCAACGAACGTTTCTGGACGTATCCGTTCGCGTGAATCGCCTCCCAATTTCAGGGAGGCGGCTTTTTCC
ATTAAAAAGGAGGAACTGTT

Downstream 100 bases:

>100_bases
CAGACGCCTTTACCGTTGGTTTTGGCGCGTATTCCATTCTTCGCGTGTTAGCCCGTAACGAATTGAGTCATAATAGACTC
CTTGGTAATAGCGAACTTTG

Product: 5'-3' exonuclease

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MANEQALLLVDGFNLLSRGYFATSYNRPIEKLERNDEGLYVNGLRVFIQKLFKLVTDYSISHCIVTWDVPREEALRRKTF
PDYKLTRNELPEPLIQQYQQCVKTLSYIGVEQLKVPPYEADDIIGAISAKWEAETSGPCYIYSNDKDLFQLLSSRTTQIV
AKKQTEVLFDEASFKASYGLSPTQWVDVKALVGDTSDNIPGCPGIGEKTALPLIAAYGSIESLYQQIDTIQPPLKRAQKK
LLAGQASTQLSKKLSAIICDIPELRHYSINDAVLSLNRGEVDKELSRLAIRVKLADELAFIS

Sequences:

>Translated_302_residues
MANEQALLLVDGFNLLSRGYFATSYNRPIEKLERNDEGLYVNGLRVFIQKLFKLVTDYSISHCIVTWDVPREEALRRKTF
PDYKLTRNELPEPLIQQYQQCVKTLSYIGVEQLKVPPYEADDIIGAISAKWEAETSGPCYIYSNDKDLFQLLSSRTTQIV
AKKQTEVLFDEASFKASYGLSPTQWVDVKALVGDTSDNIPGCPGIGEKTALPLIAAYGSIESLYQQIDTIQPPLKRAQKK
LLAGQASTQLSKKLSAIICDIPELRHYSINDAVLSLNRGEVDKELSRLAIRVKLADELAFIS
>Mature_301_residues
ANEQALLLVDGFNLLSRGYFATSYNRPIEKLERNDEGLYVNGLRVFIQKLFKLVTDYSISHCIVTWDVPREEALRRKTFP
DYKLTRNELPEPLIQQYQQCVKTLSYIGVEQLKVPPYEADDIIGAISAKWEAETSGPCYIYSNDKDLFQLLSSRTTQIVA
KKQTEVLFDEASFKASYGLSPTQWVDVKALVGDTSDNIPGCPGIGEKTALPLIAAYGSIESLYQQIDTIQPPLKRAQKKL
LAGQASTQLSKKLSAIICDIPELRHYSINDAVLSLNRGEVDKELSRLAIRVKLADELAFIS

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=260, Percent_Identity=32.3076923076923, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI226510970, Length=258, Percent_Identity=25.5813953488372, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR020047
- InterPro:   IPR008918 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 33900; Mature: 33769

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANEQALLLVDGFNLLSRGYFATSYNRPIEKLERNDEGLYVNGLRVFIQKLFKLVTDYSI
CCCCCEEEEEECHHHHHCCCEECCCCCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCC
SHCIVTWDVPREEALRRKTFPDYKLTRNELPEPLIQQYQQCVKTLSYIGVEQLKVPPYEA
CEEEEEECCCHHHHHHHCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCH
DDIIGAISAKWEAETSGPCYIYSNDKDLFQLLSSRTTQIVAKKQTEVLFDEASFKASYGL
HHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SPTQWVDVKALVGDTSDNIPGCPGIGEKTALPLIAAYGSIESLYQQIDTIQPPLKRAQKK
CCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LLAGQASTQLSKKLSAIICDIPELRHYSINDAVLSLNRGEVDKELSRLAIRVKLADELAF
HHHCCHHHHHHHHHHHHHCCCHHHHCCCCCHHEEECCCCCHHHHHHHHHHEEEEHHHHHC
IS
CC
>Mature Secondary Structure 
ANEQALLLVDGFNLLSRGYFATSYNRPIEKLERNDEGLYVNGLRVFIQKLFKLVTDYSI
CCCCEEEEEECHHHHHCCCEECCCCCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCC
SHCIVTWDVPREEALRRKTFPDYKLTRNELPEPLIQQYQQCVKTLSYIGVEQLKVPPYEA
CEEEEEECCCHHHHHHHCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCH
DDIIGAISAKWEAETSGPCYIYSNDKDLFQLLSSRTTQIVAKKQTEVLFDEASFKASYGL
HHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SPTQWVDVKALVGDTSDNIPGCPGIGEKTALPLIAAYGSIESLYQQIDTIQPPLKRAQKK
CCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LLAGQASTQLSKKLSAIICDIPELRHYSINDAVLSLNRGEVDKELSRLAIRVKLADELAF
HHHCCHHHHHHHHHHHHHCCCHHHHCCCCCHHEEECCCCCHHHHHHHHHHEEEEHHHHHC
IS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]