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The map label for this gene is ilvK [H]

Identifier: 56963696

GI number: 56963696

Start: 2046246

End: 2047328

Strand: Direct

Name: ilvK [H]

Synonym: ABC1931

Alternate gene names: 56963696

Gene position: 2046246-2047328 (Clockwise)

Preceding gene: 56963694

Following gene: 56963697

Centisome position: 47.54

GC content: 43.77

Gene sequence:

>1083_bases
ATGACAACACAAAAACTCGAAATTATGAAATGTACGCACAAAAAACAAAAACCCGAGTCTTCTGCTTTAGAGTTTGGCAA
GTATTTTACAGACCATATGTTTATCATGGATTATTCAGAAGAGAAAGGCTGGTACAGTCCCCGTATTATTCCTTATCAAC
CATTGACCCTTGATCCTGCCGCAATGGTATTCCATTATGGGCAAACGGTTTTTGAAGGCTTAAAAGCGTACCGTTCTGAA
GATGGGAGCGTCCGCTTATTCCGCCCAGAAGAAAACTTTAAGCGTTTGAATCAATCAAGCGACCGTTTGAGCATCCCGCC
GATTGATGAAGCACAAATGCTCGCTTATTTAAAGGAATTGATCCGTATCGATAAAGACTGGATTCCAACGATGCCAGGAA
CTTCCCTTTATATTCGCCCATTTGTTATTGCGACAGAACCTACATTAGGCGTAGCGCCTTCACGTAATTATAAATTTATG
ATTATCTTGTCACCAGTCGGAGCTTACTATAAAGAAGGAATCAACCCAATTGGCATTTTTGTAGAAGACCAATATACACG
TGCCGCACCAGGGGGGACTGGGACCGCGAAAACGGCTGGCAACTACTGTGCTGCTTACAAAGCACAAGAGCGGGCTACAG
CGAACAAACAAGCACAAGTTCTATGGTTGGACGGGATGGAAAAGAAATACGTCGAAGAAGTAGGCAGCATGAATGTGTTT
TTTAAATTGAACGGAGAAGTGCATACACCTGGATTATCAGGCAGCATTTTAGAGGGCATTACACGCAAAAGCGTGATCGA
GTTGCTGAAAAGTTGGGATATCCCAGTGTTCGAACGGCGGATTTCAATTGAAGAAATTGTGAGCGCAAGCAAAAGCGGTA
CGCTTGAAGAAGTCTTCGGAACTGGTACAGCAGCGGTCATCTCTCCAGTCGGAGAACTTTTGTGGAAAGACCACAAAATC
GTTGTCAACAACAATGAAACAGGAGCGTTGTCCAAACGTTTGTACACGGAATTGACAGGCATTCAAACTGGAGAACAAAA
AGACCCGTTTGGCTGGACTGTCCGTTTGGAAGACGAGGAATAA

Upstream 100 bases:

>100_bases
TCGATCAATTTCATAAAGGGTAAGCATTTTTTTGTTTGTCGTCTTGTTCCTAAACCATATCATCGATGTTACTAGAGAAA
AAATGAAGGAGCGCTCGTTC

Downstream 100 bases:

>100_bases
CCAAACCCGGAGGCAAGTATGATACGAGCTATTTTCTTTGATTTAGATGATACGTTATTATGGGATAAAAAAAGCATAAA
GGAAGCGTTTAGGCAGACAT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 2; Vegetative protein 85; VEG85 [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTTQKLEIMKCTHKKQKPESSALEFGKYFTDHMFIMDYSEEKGWYSPRIIPYQPLTLDPAAMVFHYGQTVFEGLKAYRSE
DGSVRLFRPEENFKRLNQSSDRLSIPPIDEAQMLAYLKELIRIDKDWIPTMPGTSLYIRPFVIATEPTLGVAPSRNYKFM
IILSPVGAYYKEGINPIGIFVEDQYTRAAPGGTGTAKTAGNYCAAYKAQERATANKQAQVLWLDGMEKKYVEEVGSMNVF
FKLNGEVHTPGLSGSILEGITRKSVIELLKSWDIPVFERRISIEEIVSASKSGTLEEVFGTGTAAVISPVGELLWKDHKI
VVNNNETGALSKRLYTELTGIQTGEQKDPFGWTVRLEDEE

Sequences:

>Translated_360_residues
MTTQKLEIMKCTHKKQKPESSALEFGKYFTDHMFIMDYSEEKGWYSPRIIPYQPLTLDPAAMVFHYGQTVFEGLKAYRSE
DGSVRLFRPEENFKRLNQSSDRLSIPPIDEAQMLAYLKELIRIDKDWIPTMPGTSLYIRPFVIATEPTLGVAPSRNYKFM
IILSPVGAYYKEGINPIGIFVEDQYTRAAPGGTGTAKTAGNYCAAYKAQERATANKQAQVLWLDGMEKKYVEEVGSMNVF
FKLNGEVHTPGLSGSILEGITRKSVIELLKSWDIPVFERRISIEEIVSASKSGTLEEVFGTGTAAVISPVGELLWKDHKI
VVNNNETGALSKRLYTELTGIQTGEQKDPFGWTVRLEDEE
>Mature_359_residues
TTQKLEIMKCTHKKQKPESSALEFGKYFTDHMFIMDYSEEKGWYSPRIIPYQPLTLDPAAMVFHYGQTVFEGLKAYRSED
GSVRLFRPEENFKRLNQSSDRLSIPPIDEAQMLAYLKELIRIDKDWIPTMPGTSLYIRPFVIATEPTLGVAPSRNYKFMI
ILSPVGAYYKEGINPIGIFVEDQYTRAAPGGTGTAKTAGNYCAAYKAQERATANKQAQVLWLDGMEKKYVEEVGSMNVFF
KLNGEVHTPGLSGSILEGITRKSVIELLKSWDIPVFERRISIEEIVSASKSGTLEEVFGTGTAAVISPVGELLWKDHKIV
VNNNETGALSKRLYTELTGIQTGEQKDPFGWTVRLEDEE

Specific function: Transaminates branched-chain amino acids and ketoglutarate [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=361, Percent_Identity=38.781163434903, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI38176287, Length=361, Percent_Identity=38.781163434903, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI296010906, Length=361, Percent_Identity=38.781163434903, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI50658084, Length=349, Percent_Identity=40.974212034384, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI296010902, Length=364, Percent_Identity=34.6153846153846, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI296010900, Length=361, Percent_Identity=34.6260387811634, Blast_Score=214, Evalue=9e-56,
Organism=Homo sapiens, GI258614015, Length=284, Percent_Identity=39.7887323943662, Blast_Score=207, Evalue=9e-54,
Organism=Escherichia coli, GI48994963, Length=317, Percent_Identity=30.5993690851735, Blast_Score=125, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17568601, Length=349, Percent_Identity=38.10888252149, Blast_Score=247, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI17565728, Length=347, Percent_Identity=36.3112391930836, Blast_Score=207, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6322608, Length=360, Percent_Identity=40.2777777777778, Blast_Score=263, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6322002, Length=359, Percent_Identity=39.8328690807799, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24641779, Length=351, Percent_Identity=39.3162393162393, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 40552; Mature: 40421

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQKLEIMKCTHKKQKPESSALEFGKYFTDHMFIMDYSEEKGWYSPRIIPYQPLTLDPA
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCEEEEEEECCCCCCCCCEECCCCCCCCCHH
AMVFHYGQTVFEGLKAYRSEDGSVRLFRPEENFKRLNQSSDRLSIPPIDEAQMLAYLKEL
HHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
IRIDKDWIPTMPGTSLYIRPFVIATEPTLGVAPSRNYKFMIILSPVGAYYKEGINPIGIF
HHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHCCCCEEEEE
VEDQYTRAAPGGTGTAKTAGNYCAAYKAQERATANKQAQVLWLDGMEKKYVEEVGSMNVF
EECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCEEEE
FKLNGEVHTPGLSGSILEGITRKSVIELLKSWDIPVFERRISIEEIVSASKSGTLEEVFG
EEECCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHCCCCCCHHHHHC
TGTAAVISPVGELLWKDHKIVVNNNETGALSKRLYTELTGIQTGEQKDPFGWTVRLEDEE
CCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
TTQKLEIMKCTHKKQKPESSALEFGKYFTDHMFIMDYSEEKGWYSPRIIPYQPLTLDPA
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHCEEEEEEECCCCCCCCCEECCCCCCCCCHH
AMVFHYGQTVFEGLKAYRSEDGSVRLFRPEENFKRLNQSSDRLSIPPIDEAQMLAYLKEL
HHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
IRIDKDWIPTMPGTSLYIRPFVIATEPTLGVAPSRNYKFMIILSPVGAYYKEGINPIGIF
HHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHCCCCEEEEE
VEDQYTRAAPGGTGTAKTAGNYCAAYKAQERATANKQAQVLWLDGMEKKYVEEVGSMNVF
EECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCEEEE
FKLNGEVHTPGLSGSILEGITRKSVIELLKSWDIPVFERRISIEEIVSASKSGTLEEVFG
EEECCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHCCCCCCHHHHHC
TGTAAVISPVGELLWKDHKIVVNNNETGALSKRLYTELTGIQTGEQKDPFGWTVRLEDEE
CCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 7934828; 9298659 [H]