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The map label for this gene is trpA

Identifier: 56963665

GI number: 56963665

Start: 2016865

End: 2017674

Strand: Direct

Name: trpA

Synonym: ABC1900

Alternate gene names: 56963665

Gene position: 2016865-2017674 (Clockwise)

Preceding gene: 56963664

Following gene: 56963666

Centisome position: 46.86

GC content: 49.38

Gene sequence:

>810_bases
ATGAATAAGCGGCTTCGTGATGCTGTAAAAAAGGGCAACTTGTTTATTCCGTTCATTACCGCCGGAGACCCGGTGCGAGA
AGCAACCATCGCCCTCGCACTCTCATTACAGAGAAGTGGGGCGGATGTGCTGGAGCTTGGCATTCCTTATTCTGACCCGT
TGGCAGATGGACCGGTCATACAAAACGCCTCCAAACGTGCACTAGCAGGAGGAATGACACTGGCTAAGGCGATGGCGCTC
GTCCCGGAAATGAGAAAAGAGGGGCTTACAATTCCTGTTATTGTCTTTACTTACGCCAACCCTTTGCTACAATTTGGGTT
TGAACGTTTTTGTGAGACCGCATGCGACTACGGAATTGACGGCCTCCTCGTTCCCGATTTGCCGTTTGAAGAGAGCGAAA
CATTGGCTAATGAGTGTGAAAAACAAGGGCTTGCCCTTATATCACTTGTGGCGCCAACATCTCAGCAACGGATCAAAGCG
ATTGCCAGCCGCGCCCAAGGGTTCCTATACTGTGTGTCCTCGCTAGGCGTAACTGGCGCGAGAAAAACACTTCATCCACA
AGTAGAAGCTTTTTTAAAGCTGGTAAAAGAAGCATCGCCTGTCCCGTTTGTAGTTGGATTTGGCATTTCCAGCTACGAAC
AAGTTGAGGAAATGGGGCGCCATGCTGACGGTGTTGTGATCGGGAGTGCGATTGTTGAGCAAATTGGCCTCAGATGCGAG
GCGTTAAAACAGGCGGAATCACGAGATGAAGCAGTCAAAGAAATTGAGCAATATGTCAATTCTCTTCTTCATCCCGCTCC
GACCTATTAA

Upstream 100 bases:

>100_bases
GCGAAAACGATGACGCCAAACCAATCTGTACTCGTATGTTTATCAGGCAGAGGCGATAAGGATATGCACACATTGCAACG
GGTTTATAAGGAGGGGTACG

Downstream 100 bases:

>100_bases
ACAGTTGCTAGTACGCTTAATTTTGCGTAGGATAGCTAGTAAGACTAGGGAAAGCAGGTGCAGACACGATGTTAGCAAAA
AAACAAATACAAGGGTTGCC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNKRLRDAVKKGNLFIPFITAGDPVREATIALALSLQRSGADVLELGIPYSDPLADGPVIQNASKRALAGGMTLAKAMAL
VPEMRKEGLTIPVIVFTYANPLLQFGFERFCETACDYGIDGLLVPDLPFEESETLANECEKQGLALISLVAPTSQQRIKA
IASRAQGFLYCVSSLGVTGARKTLHPQVEAFLKLVKEASPVPFVVGFGISSYEQVEEMGRHADGVVIGSAIVEQIGLRCE
ALKQAESRDEAVKEIEQYVNSLLHPAPTY

Sequences:

>Translated_269_residues
MNKRLRDAVKKGNLFIPFITAGDPVREATIALALSLQRSGADVLELGIPYSDPLADGPVIQNASKRALAGGMTLAKAMAL
VPEMRKEGLTIPVIVFTYANPLLQFGFERFCETACDYGIDGLLVPDLPFEESETLANECEKQGLALISLVAPTSQQRIKA
IASRAQGFLYCVSSLGVTGARKTLHPQVEAFLKLVKEASPVPFVVGFGISSYEQVEEMGRHADGVVIGSAIVEQIGLRCE
ALKQAESRDEAVKEIEQYVNSLLHPAPTY
>Mature_269_residues
MNKRLRDAVKKGNLFIPFITAGDPVREATIALALSLQRSGADVLELGIPYSDPLADGPVIQNASKRALAGGMTLAKAMAL
VPEMRKEGLTIPVIVFTYANPLLQFGFERFCETACDYGIDGLLVPDLPFEESETLANECEKQGLALISLVAPTSQQRIKA
IASRAQGFLYCVSSLGVTGARKTLHPQVEAFLKLVKEASPVPFVVGFGISSYEQVEEMGRHADGVVIGSAIVEQIGLRCE
ALKQAESRDEAVKEIEQYVNSLLHPAPTY

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=228, Percent_Identity=37.719298245614, Blast_Score=142, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321412, Length=256, Percent_Identity=38.28125, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_BACSK (Q5WGS0)

Other databases:

- EMBL:   AP006627
- RefSeq:   YP_175396.1
- ProteinModelPortal:   Q5WGS0
- SMR:   Q5WGS0
- STRING:   Q5WGS0
- EnsemblBacteria:   EBBACT00000047235
- GeneID:   3203560
- GenomeReviews:   AP006627_GR
- KEGG:   bcl:ABC1900
- NMPDR:   fig|66692.3.peg.1944
- eggNOG:   COG0159
- GeneTree:   EBGT00050000001942
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   BCLA66692:ABC1900-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29049; Mature: 29049

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 45-45 ACT_SITE 56-56

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRLRDAVKKGNLFIPFITAGDPVREATIALALSLQRSGADVLELGIPYSDPLADGPVI
CCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHH
QNASKRALAGGMTLAKAMALVPEMRKEGLTIPVIVFTYANPLLQFGFERFCETACDYGID
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLLVPDLPFEESETLANECEKQGLALISLVAPTSQQRIKAIASRAQGFLYCVSSLGVTGA
CEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
RKTLHPQVEAFLKLVKEASPVPFVVGFGISSYEQVEEMGRHADGVVIGSAIVEQIGLRCE
HHHCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCCCCEEHHHHHHHHHCCHHH
ALKQAESRDEAVKEIEQYVNSLLHPAPTY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNKRLRDAVKKGNLFIPFITAGDPVREATIALALSLQRSGADVLELGIPYSDPLADGPVI
CCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHH
QNASKRALAGGMTLAKAMALVPEMRKEGLTIPVIVFTYANPLLQFGFERFCETACDYGID
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLLVPDLPFEESETLANECEKQGLALISLVAPTSQQRIKAIASRAQGFLYCVSSLGVTGA
CEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
RKTLHPQVEAFLKLVKEASPVPFVVGFGISSYEQVEEMGRHADGVVIGSAIVEQIGLRCE
HHHCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCCCCEEHHHHHHHHHCCHHH
ALKQAESRDEAVKEIEQYVNSLLHPAPTY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA