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The map label for this gene is metI [H]

Identifier: 56963632

GI number: 56963632

Start: 1983395

End: 1984501

Strand: Direct

Name: metI [H]

Synonym: ABC1867

Alternate gene names: 56963632

Gene position: 1983395-1984501 (Clockwise)

Preceding gene: 56963631

Following gene: 56963633

Centisome position: 46.08

GC content: 46.79

Gene sequence:

>1107_bases
ATGGAAAAACGAGCTGAAACCATCTTAGCGCAAATTGGCAATCGCCGCGATGAACATACTGGCGCAGTAAACACGCCTGT
TTATTTTTCAACAGCATATCGCCATCCAGGTATTGGCGAATCGACTGGGTATGATTATGCCCGTACAGGCAATCCGACAA
GGGACGTGCTCGAAAAAGCGATTGCGGAATTAGAAGAAGGTGAGCGCGGATTTGCGACTAGCTCTGGCATGGCTGCTGTT
CAAATTGTGCTTTCGTTGTTTGAGCAAGGCGATGGGATCATCTGTTCGAAAGATTTATATGGCGGTACGTACCGTCTGTT
TGAAGGAGGCTGGACAAGGTGGGGCGTGTCCTTTACATATGTGGATCCACGGAATTTGCAAGAAGTCGAACAAGCCATTC
ATTCTAACGTGAAAGCCATTTTTATCGAGACGCCGACTAACCCACTTATGCAGGAAGCCTCGATTCCAGCGCTTGCTGCT
CTCGCCAAAAAACACGATTTGCTGCTCATTGTCGATAACACATTTTATACGCCGCTTTTGCAGAAGCCACTAACGGAAGG
AGCAGACATCGTGATCCATTCGGCGACAAAGTACTTAGGAGGCCATAACGATGTCGTTGCTGGGCTTATCGTTGCGAAAG
GAGCCGATCTTTGTGAACGGCTCGCTTATTATCATAATGGTGCTGGTGGTATTTTAAGTGCATTTGATTCTTGGCTGCTT
ATTCGAGGAATGAAGACATTGGCATTGCGGATGGCCAAGCATGAGGAAAACGCTAAAAAAGTGGTGCATGCATTGGAGCA
AACAGACGGCATTGTAGACGTGCTTTATCCTGGGAGAGGCGGAATGCTGTCGTTTCGTGTTCAAAACGAAGCTTGGGTCA
ATCCGTTGCTAAAACATTTGCAATTAATTTCGTTCGCGGAAAGCCTCGGTGGTGTCGAAAGTTTAATGACATATCCAGCA
ACACAAACGCACGCTGATATTCCCGAAGAGATCAGACTTGCAAATGGAGTGGATAATGGATTGCTCCGTTTCTCAGTCGG
CATTGAAAACGGAGAAGATATTGTAGCTGATTTGCTGCAAGCAATTGCGGCCGCTAAAAAATCCTAG

Upstream 100 bases:

>100_bases
TATCTGCAAGATAAGAGAAGCATGCACGTAAGCAACCCCTTCTTCTAATCTTGGAGAAGGGTTTTTTGTTTGGCAATAGA
ACACATTGGGGGGAACTAGA

Downstream 100 bases:

>100_bases
GAGGGAATACGATGGAACTTCGCGATAAGTTAAAAACGAGCTTAATCGTTGGAGATGGGGCTATGGGCACGCTTTTATAC
GAGCAAGGTTTTGATTTATG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-acetylhomoserine (thiol)-lyase; O-acetylhomoserine sulfhydrylase; OAH sulfhydrylase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MEKRAETILAQIGNRRDEHTGAVNTPVYFSTAYRHPGIGESTGYDYARTGNPTRDVLEKAIAELEEGERGFATSSGMAAV
QIVLSLFEQGDGIICSKDLYGGTYRLFEGGWTRWGVSFTYVDPRNLQEVEQAIHSNVKAIFIETPTNPLMQEASIPALAA
LAKKHDLLLIVDNTFYTPLLQKPLTEGADIVIHSATKYLGGHNDVVAGLIVAKGADLCERLAYYHNGAGGILSAFDSWLL
IRGMKTLALRMAKHEENAKKVVHALEQTDGIVDVLYPGRGGMLSFRVQNEAWVNPLLKHLQLISFAESLGGVESLMTYPA
TQTHADIPEEIRLANGVDNGLLRFSVGIENGEDIVADLLQAIAAAKKS

Sequences:

>Translated_368_residues
MEKRAETILAQIGNRRDEHTGAVNTPVYFSTAYRHPGIGESTGYDYARTGNPTRDVLEKAIAELEEGERGFATSSGMAAV
QIVLSLFEQGDGIICSKDLYGGTYRLFEGGWTRWGVSFTYVDPRNLQEVEQAIHSNVKAIFIETPTNPLMQEASIPALAA
LAKKHDLLLIVDNTFYTPLLQKPLTEGADIVIHSATKYLGGHNDVVAGLIVAKGADLCERLAYYHNGAGGILSAFDSWLL
IRGMKTLALRMAKHEENAKKVVHALEQTDGIVDVLYPGRGGMLSFRVQNEAWVNPLLKHLQLISFAESLGGVESLMTYPA
TQTHADIPEEIRLANGVDNGLLRFSVGIENGEDIVADLLQAIAAAKKS
>Mature_368_residues
MEKRAETILAQIGNRRDEHTGAVNTPVYFSTAYRHPGIGESTGYDYARTGNPTRDVLEKAIAELEEGERGFATSSGMAAV
QIVLSLFEQGDGIICSKDLYGGTYRLFEGGWTRWGVSFTYVDPRNLQEVEQAIHSNVKAIFIETPTNPLMQEASIPALAA
LAKKHDLLLIVDNTFYTPLLQKPLTEGADIVIHSATKYLGGHNDVVAGLIVAKGADLCERLAYYHNGAGGILSAFDSWLL
IRGMKTLALRMAKHEENAKKVVHALEQTDGIVDVLYPGRGGMLSFRVQNEAWVNPLLKHLQLISFAESLGGVESLMTYPA
TQTHADIPEEIRLANGVDNGLLRFSVGIENGEDIVADLLQAIAAAKKS

Specific function: Catalyzes the transformation of O-succinylhomoserine into cystathionine, as well as O-acetylhomoserine and sulfide into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=362, Percent_Identity=40.0552486187845, Blast_Score=255, Evalue=6e-68,
Organism=Homo sapiens, GI299473759, Length=362, Percent_Identity=36.1878453038674, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI25453487, Length=361, Percent_Identity=34.9030470914127, Blast_Score=198, Evalue=6e-51,
Organism=Escherichia coli, GI1790375, Length=386, Percent_Identity=41.1917098445596, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1789383, Length=394, Percent_Identity=28.4263959390863, Blast_Score=135, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17560056, Length=363, Percent_Identity=40.771349862259, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17538055, Length=363, Percent_Identity=40.495867768595, Blast_Score=264, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI71980708, Length=369, Percent_Identity=36.3143631436314, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319307, Length=378, Percent_Identity=39.9470899470899, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6321254, Length=405, Percent_Identity=33.3333333333333, Blast_Score=202, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6323333, Length=408, Percent_Identity=32.843137254902, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321170, Length=328, Percent_Identity=30.4878048780488, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321904, Length=382, Percent_Identity=27.7486910994764, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922554, Length=368, Percent_Identity=41.0326086956522, Blast_Score=281, Evalue=6e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48; =2.5.1.49 [H]

Molecular weight: Translated: 40094; Mature: 40094

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRAETILAQIGNRRDEHTGAVNTPVYFSTAYRHPGIGESTGYDYARTGNPTRDVLEKA
CCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
IAELEEGERGFATSSGMAAVQIVLSLFEQGDGIICSKDLYGGTYRLFEGGWTRWGVSFTY
HHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCEEECEEEEE
VDPRNLQEVEQAIHSNVKAIFIETPTNPLMQEASIPALAALAKKHDLLLIVDNTFYTPLL
ECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHCCCHHHHHHHCCCCEEEEECCCCCCHHH
QKPLTEGADIVIHSATKYLGGHNDVVAGLIVAKGADLCERLAYYHNGAGGILSAFDSWLL
HHHCCCCCCEEEECHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHH
IRGMKTLALRMAKHEENAKKVVHALEQTDGIVDVLYPGRGGMLSFRVQNEAWVNPLLKHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCHHHHHHHHHHH
QLISFAESLGGVESLMTYPATQTHADIPEEIRLANGVDNGLLRFSVGIENGEDIVADLLQ
HHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHH
AIAAAKKS
HHHHHCCC
>Mature Secondary Structure
MEKRAETILAQIGNRRDEHTGAVNTPVYFSTAYRHPGIGESTGYDYARTGNPTRDVLEKA
CCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
IAELEEGERGFATSSGMAAVQIVLSLFEQGDGIICSKDLYGGTYRLFEGGWTRWGVSFTY
HHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCEEECEEEEE
VDPRNLQEVEQAIHSNVKAIFIETPTNPLMQEASIPALAALAKKHDLLLIVDNTFYTPLL
ECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHCCCHHHHHHHCCCCEEEEECCCCCCHHH
QKPLTEGADIVIHSATKYLGGHNDVVAGLIVAKGADLCERLAYYHNGAGGILSAFDSWLL
HHHCCCCCCEEEECHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHH
IRGMKTLALRMAKHEENAKKVVHALEQTDGIVDVLYPGRGGMLSFRVQNEAWVNPLLKHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCHHHHHHHHHHH
QLISFAESLGGVESLMTYPATQTHADIPEEIRLANGVDNGLLRFSVGIENGEDIVADLLQ
HHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHH
AIAAAKKS
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]