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The map label for this gene is yitJ [H]

Identifier: 56963633

GI number: 56963633

Start: 1984513

End: 1986378

Strand: Direct

Name: yitJ [H]

Synonym: ABC1868

Alternate gene names: 56963633

Gene position: 1984513-1986378 (Clockwise)

Preceding gene: 56963632

Following gene: 56963634

Centisome position: 46.11

GC content: 46.14

Gene sequence:

>1866_bases
ATGGAACTTCGCGATAAGTTAAAAACGAGCTTAATCGTTGGAGATGGGGCTATGGGCACGCTTTTATACGAGCAAGGTTT
TGATTTATGTTACGAAGAATTAAACCTTTCCCAGCCAGACGCTGTTTACCATGTGCATCGATCGTATATTGAGGCAGGTG
CGAAACTGCTACAAACCAATACGTATGCAGCAAATGCCGTCAAACTTAAAAAATATGGATTAAACGAAAGTGTGGCAGAC
ATCAATAGCGCCGCCGTCGGCATTGCGAAAAAAGCGATTGGCAACAACGAACAGCTTGCGTTAGTTGGCACAATCGGCGG
GATTCGCGGGTTCCTCCATGAAGATACGGAAAACAAGGTGATTACAGCTGCTTTACTTGAACAAATAACGGCCCTCTTGG
AAGCAGGCGTTGACGGGTTATTGTTTGAAACGTTTTATGATTTTGAAGAGGCAAAAGAAGCAGTAGCCCTGGCAAGGCGT
CTTACAGACAAGGCGATTATTATGAACGTGTCGATGGGCGATATCGGCGTCTTAAATGGCGGCATTCCCCTAGGTGAAGC
GCTCGAGCAACTGCATGAATTAGGAGCGGACGTTTTAGGAATCAATTGCCGTATGGGCCCTTATCATATGCTCCGCTCTT
TGGAACAGCTTGAATTATCGTCAGCTTTTCCACTTGCTTGCTATCCAAATGCCAGTTTGCCAGGTTACCGGGACGGGCGC
TTTGTTTATGCTTCCAATACCGATTACTTTTATGAAATGACAGGGGCGTTTATTGAACAAGGCGTCCATTTGATTGGCGG
CTGCTGTGGCACAACGCCTGCACATATTGCCGCGATCGCCAAAGCAGTCGAAACGAACACGCCGAAAACACGCTCGCTCA
TAGTCAGACGACCAAGCCAACTTCAGGAGGAAGGACCAGGAAGAGGAGAGCGGCTCAATGAGCCGTTGGACCAATTGGCA
AGGCGCAAACAAACGATTATTGTTGAACTTGATCCGCCGAAAAAATTAATGATTGCGAAATTTATGGAAGGAGCCAAAGC
GTTAAAAGAGGCAGGGGTAGATGCGTTAACGCTTGCCGACAATTCTCTTGCTAACCCTCGTGTTGACAACCAAACGTTGG
CGATGTTAGTAAGGGAACAGTTAGGGTTGCGTAGCCTTGTCCACTTAACATGCCGTGACCGCAATTTGATTGGCATGCAA
TCACACTTAATGGGGCTCCATCTAGCGGAGCTTCATGATTTACTTGTTATCACTGGCGATCCAAGCAAGATCGGTGATTT
TCCCGGAGCAACATCTGTATATGATGTATCTTCGTTAAAATTGTTGCCTTTAATTAAGCAAATGAATGAAGGGATTTCAT
TCTCTGGAAACTCCCTTGGCAGTAAAGCAACTTTTTCGACAGCGGCAGCGTTTAATCCCCATGTTAAGCATCTTGATAAA
GCGGTGCGACGTTTAGAAAAGAAAATCGAAGCTGGCGCTGATTATTTTCTGACACAGCCCATTTTCGATGAACAACAATT
TGAACAATTGTATGACGCAACGAAGCATTTGCCTGTGCCGATTTTTGTTGGCATTATGCCGCTAACAAGCAGCCGCAATG
CGGAGTTTCTTCATAATGAAGTGCCAGGAATGACATTGAGCGACGAGACAAGAGCACGGATGGCGGCATGTGGAAACGAC
AAAGAAAAAGCCGAGCAAGAAGGTTTGCAAATTGCGATGGAGCTAGCAGACGCAGCTAGTGCCTATTTTAACGGCTTGTA
TTTAGTCACACCGTTTTTACGTTATGAATTAACTGCAGCACTTGCCACCCATTGCCGAGCGATAGCGAACCCGATAAACC
ACACGATGGAAGGCGAACGAATATGA

Upstream 100 bases:

>100_bases
GATTGCTCCGTTTCTCAGTCGGCATTGAAAACGGAGAAGATATTGTAGCTGATTTGCTGCAAGCAATTGCGGCCGCTAAA
AAATCCTAGGAGGGAATACG

Downstream 100 bases:

>100_bases
CAGAATCTCTATTTGAAAAGCAGCTAAAGCAATCGATCCTTGTGCTAGATGGAGCAATGGGGACGATGTTGCAAGAAGCA
AACTTGACCGCAGCCGATTT

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MELRDKLKTSLIVGDGAMGTLLYEQGFDLCYEELNLSQPDAVYHVHRSYIEAGAKLLQTNTYAANAVKLKKYGLNESVAD
INSAAVGIAKKAIGNNEQLALVGTIGGIRGFLHEDTENKVITAALLEQITALLEAGVDGLLFETFYDFEEAKEAVALARR
LTDKAIIMNVSMGDIGVLNGGIPLGEALEQLHELGADVLGINCRMGPYHMLRSLEQLELSSAFPLACYPNASLPGYRDGR
FVYASNTDYFYEMTGAFIEQGVHLIGGCCGTTPAHIAAIAKAVETNTPKTRSLIVRRPSQLQEEGPGRGERLNEPLDQLA
RRKQTIIVELDPPKKLMIAKFMEGAKALKEAGVDALTLADNSLANPRVDNQTLAMLVREQLGLRSLVHLTCRDRNLIGMQ
SHLMGLHLAELHDLLVITGDPSKIGDFPGATSVYDVSSLKLLPLIKQMNEGISFSGNSLGSKATFSTAAAFNPHVKHLDK
AVRRLEKKIEAGADYFLTQPIFDEQQFEQLYDATKHLPVPIFVGIMPLTSSRNAEFLHNEVPGMTLSDETRARMAACGND
KEKAEQEGLQIAMELADAASAYFNGLYLVTPFLRYELTAALATHCRAIANPINHTMEGERI

Sequences:

>Translated_621_residues
MELRDKLKTSLIVGDGAMGTLLYEQGFDLCYEELNLSQPDAVYHVHRSYIEAGAKLLQTNTYAANAVKLKKYGLNESVAD
INSAAVGIAKKAIGNNEQLALVGTIGGIRGFLHEDTENKVITAALLEQITALLEAGVDGLLFETFYDFEEAKEAVALARR
LTDKAIIMNVSMGDIGVLNGGIPLGEALEQLHELGADVLGINCRMGPYHMLRSLEQLELSSAFPLACYPNASLPGYRDGR
FVYASNTDYFYEMTGAFIEQGVHLIGGCCGTTPAHIAAIAKAVETNTPKTRSLIVRRPSQLQEEGPGRGERLNEPLDQLA
RRKQTIIVELDPPKKLMIAKFMEGAKALKEAGVDALTLADNSLANPRVDNQTLAMLVREQLGLRSLVHLTCRDRNLIGMQ
SHLMGLHLAELHDLLVITGDPSKIGDFPGATSVYDVSSLKLLPLIKQMNEGISFSGNSLGSKATFSTAAAFNPHVKHLDK
AVRRLEKKIEAGADYFLTQPIFDEQQFEQLYDATKHLPVPIFVGIMPLTSSRNAEFLHNEVPGMTLSDETRARMAACGND
KEKAEQEGLQIAMELADAASAYFNGLYLVTPFLRYELTAALATHCRAIANPINHTMEGERI
>Mature_621_residues
MELRDKLKTSLIVGDGAMGTLLYEQGFDLCYEELNLSQPDAVYHVHRSYIEAGAKLLQTNTYAANAVKLKKYGLNESVAD
INSAAVGIAKKAIGNNEQLALVGTIGGIRGFLHEDTENKVITAALLEQITALLEAGVDGLLFETFYDFEEAKEAVALARR
LTDKAIIMNVSMGDIGVLNGGIPLGEALEQLHELGADVLGINCRMGPYHMLRSLEQLELSSAFPLACYPNASLPGYRDGR
FVYASNTDYFYEMTGAFIEQGVHLIGGCCGTTPAHIAAIAKAVETNTPKTRSLIVRRPSQLQEEGPGRGERLNEPLDQLA
RRKQTIIVELDPPKKLMIAKFMEGAKALKEAGVDALTLADNSLANPRVDNQTLAMLVREQLGLRSLVHLTCRDRNLIGMQ
SHLMGLHLAELHDLLVITGDPSKIGDFPGATSVYDVSSLKLLPLIKQMNEGISFSGNSLGSKATFSTAAAFNPHVKHLDK
AVRRLEKKIEAGADYFLTQPIFDEQQFEQLYDATKHLPVPIFVGIMPLTSSRNAEFLHNEVPGMTLSDETRARMAACGND
KEKAEQEGLQIAMELADAASAYFNGLYLVTPFLRYELTAALATHCRAIANPINHTMEGERI

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=337, Percent_Identity=28.1899109792285, Blast_Score=109, Evalue=9e-24,
Organism=Homo sapiens, GI13162290, Length=291, Percent_Identity=27.1477663230241, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI157266337, Length=296, Percent_Identity=29.0540540540541, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1790450, Length=333, Percent_Identity=27.027027027027, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1786456, Length=300, Percent_Identity=28.3333333333333, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535405, Length=333, Percent_Identity=28.2282282282282, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322966, Length=312, Percent_Identity=24.6794871794872, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24585077, Length=285, Percent_Identity=25.9649122807018, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 67801; Mature: 67801

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRDKLKTSLIVGDGAMGTLLYEQGFDLCYEELNLSQPDAVYHVHRSYIEAGAKLLQTN
CCHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
TYAANAVKLKKYGLNESVADINSAAVGIAKKAIGNNEQLALVGTIGGIRGFLHEDTENKV
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCCHH
ITAALLEQITALLEAGVDGLLFETFYDFEEAKEAVALARRLTDKAIIMNVSMGDIGVLNG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEECC
GIPLGEALEQLHELGADVLGINCRMGPYHMLRSLEQLELSSAFPLACYPNASLPGYRDGR
CCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCE
FVYASNTDYFYEMTGAFIEQGVHLIGGCCGTTPAHIAAIAKAVETNTPKTRSLIVRRPSQ
EEEECCCCEEHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHEEECCHH
LQEEGPGRGERLNEPLDQLARRKQTIIVELDPPKKLMIAKFMEGAKALKEAGVDALTLAD
HHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEECC
NSLANPRVDNQTLAMLVREQLGLRSLVHLTCRDRNLIGMQSHLMGLHLAELHDLLVITGD
CCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEECC
PSKIGDFPGATSVYDVSSLKLLPLIKQMNEGISFSGNSLGSKATFSTAAAFNPHVKHLDK
CHHCCCCCCCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHH
AVRRLEKKIEAGADYFLTQPIFDEQQFEQLYDATKHLPVPIFVGIMPLTSSRNAEFLHNE
HHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHC
VPGMTLSDETRARMAACGNDKEKAEQEGLQIAMELADAASAYFNGLYLVTPFLRYELTAA
CCCCEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHH
LATHCRAIANPINHTMEGERI
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MELRDKLKTSLIVGDGAMGTLLYEQGFDLCYEELNLSQPDAVYHVHRSYIEAGAKLLQTN
CCHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
TYAANAVKLKKYGLNESVADINSAAVGIAKKAIGNNEQLALVGTIGGIRGFLHEDTENKV
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCCHH
ITAALLEQITALLEAGVDGLLFETFYDFEEAKEAVALARRLTDKAIIMNVSMGDIGVLNG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEECC
GIPLGEALEQLHELGADVLGINCRMGPYHMLRSLEQLELSSAFPLACYPNASLPGYRDGR
CCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCE
FVYASNTDYFYEMTGAFIEQGVHLIGGCCGTTPAHIAAIAKAVETNTPKTRSLIVRRPSQ
EEEECCCCEEHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHEEECCHH
LQEEGPGRGERLNEPLDQLARRKQTIIVELDPPKKLMIAKFMEGAKALKEAGVDALTLAD
HHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEECC
NSLANPRVDNQTLAMLVREQLGLRSLVHLTCRDRNLIGMQSHLMGLHLAELHDLLVITGD
CCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEECC
PSKIGDFPGATSVYDVSSLKLLPLIKQMNEGISFSGNSLGSKATFSTAAAFNPHVKHLDK
CHHCCCCCCCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHH
AVRRLEKKIEAGADYFLTQPIFDEQQFEQLYDATKHLPVPIFVGIMPLTSSRNAEFLHNE
HHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHC
VPGMTLSDETRARMAACGNDKEKAEQEGLQIAMELADAASAYFNGLYLVTPFLRYELTAA
CCCCEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHH
LATHCRAIANPINHTMEGERI
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]