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The map label for this gene is goxB [H]

Identifier: 56963501

GI number: 56963501

Start: 1862741

End: 1863847

Strand: Direct

Name: goxB [H]

Synonym: ABC1736

Alternate gene names: 56963501

Gene position: 1862741-1863847 (Clockwise)

Preceding gene: 56963500

Following gene: 56963502

Centisome position: 43.28

GC content: 48.06

Gene sequence:

>1107_bases
ATGGAGCATACAATTGTTTTAGGAGGCGGCGTTATCGGGCTGTCTGTTGCTTTTCAGTTAGCAATGGACGGCAAGACCGT
TTCTGTCCTAGAAATAAACACATGCGGCGGGCAGGCATCTGGTGCAGCTGCAGGCATGCTTGCCCCGTACTCAGAAATTA
GCGAGGATCCAGATGATTTCTTTCGCCTTTGTTTAGCAAGTTTGCGCGCTTTTAAAGAATGGCAAGCATCCGTAAAACAA
GAGTCAGGCATGACGTTTGAATTTACAGAATCAGGGAGTCTTCATTGTGTGTATCATGAAGCTGATTTGTTGTCACTTGC
CACTAGAAAAGCATGGCAAGAGGAATTTGGGGCCAAAGTCGACGTATTGACACGCGAAGAGTTAAAGAAAAAAGAGCCTG
AGCTTGCCGAAGACATAATTGGTGCGATCCACTACCCAGAAGAAGCTCATGTCTATGCGCCTGATTATGTAAAGGCGTTG
CAACAGGCTTGCAGAAATCGCGGCGTCCACATTTATGAGCAGCTCAAACACGTTTCGCTCGTAAAAACAGAGCCCCACAT
CGAGCTACAGAGCGCTTCAGGGCAGCGTTTCACAGCAGACCAGCTCGTTGTGGCGACAGGTGCATGGGCGAAAGAGCTAG
AAAGCCAGCTTGGCTTGAACTTGCCCATTTACCCAATAAGAGGGCAAATTTGTGCTTATAACATTGAACCAGGCCGTATT
CGCCACATTTTGTACACAAGCCAAGGGTATCTTGTCCCAAAAGCAAATGGCACACTTGTTAATGGCGCTTCAGAAGATAT
TGCTGGCTTTCAAACAGAAATTACCGACAAGGGCATTCGCCGTCTAACAAATTGGAACCATAAGATAGTGCCCTTTTTAG
CTGAACTCACGCCGTTTCATAAATGGGCTGGCTTAAGGCCGGCGACACAAGACGGTTATCCGCTTATCGGTTTTCTTCGC
AACCATCCCCGCGTTTTTATGGCTTGTGGCCATTACCGGAACGGCATTTTATTAAGCGCGATTACGGCAAAAGTGGCGAG
TGCGCAGTTGGCTGGGCAGAAGCCGCCTGTACCAATCGCCCTTTTTGATCCAGACCGTTTTTCGTAG

Upstream 100 bases:

>100_bases
TGCTGTCCTACGAAGCAGGGCGCATTCCGATGAAGCGCTATGCGACAGCAAGCAGCCAGCTTGACCATTTATTGTCCAGT
CACAGTAAGGGGTAGTCAAA

Downstream 100 bases:

>100_bases
TCAGAGAAGGAGGCGTACGAATGCCCATTCCAACAGTCCTCTCCGTTGCTGGTTCTGATTCCAGCGGCGGTGCTGGCATT
CAAGCAGACATAAAAACGTG

Product: glycine oxidase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MEHTIVLGGGVIGLSVAFQLAMDGKTVSVLEINTCGGQASGAAAGMLAPYSEISEDPDDFFRLCLASLRAFKEWQASVKQ
ESGMTFEFTESGSLHCVYHEADLLSLATRKAWQEEFGAKVDVLTREELKKKEPELAEDIIGAIHYPEEAHVYAPDYVKAL
QQACRNRGVHIYEQLKHVSLVKTEPHIELQSASGQRFTADQLVVATGAWAKELESQLGLNLPIYPIRGQICAYNIEPGRI
RHILYTSQGYLVPKANGTLVNGASEDIAGFQTEITDKGIRRLTNWNHKIVPFLAELTPFHKWAGLRPATQDGYPLIGFLR
NHPRVFMACGHYRNGILLSAITAKVASAQLAGQKPPVPIALFDPDRFS

Sequences:

>Translated_368_residues
MEHTIVLGGGVIGLSVAFQLAMDGKTVSVLEINTCGGQASGAAAGMLAPYSEISEDPDDFFRLCLASLRAFKEWQASVKQ
ESGMTFEFTESGSLHCVYHEADLLSLATRKAWQEEFGAKVDVLTREELKKKEPELAEDIIGAIHYPEEAHVYAPDYVKAL
QQACRNRGVHIYEQLKHVSLVKTEPHIELQSASGQRFTADQLVVATGAWAKELESQLGLNLPIYPIRGQICAYNIEPGRI
RHILYTSQGYLVPKANGTLVNGASEDIAGFQTEITDKGIRRLTNWNHKIVPFLAELTPFHKWAGLRPATQDGYPLIGFLR
NHPRVFMACGHYRNGILLSAITAKVASAQLAGQKPPVPIALFDPDRFS
>Mature_368_residues
MEHTIVLGGGVIGLSVAFQLAMDGKTVSVLEINTCGGQASGAAAGMLAPYSEISEDPDDFFRLCLASLRAFKEWQASVKQ
ESGMTFEFTESGSLHCVYHEADLLSLATRKAWQEEFGAKVDVLTREELKKKEPELAEDIIGAIHYPEEAHVYAPDYVKAL
QQACRNRGVHIYEQLKHVSLVKTEPHIELQSASGQRFTADQLVVATGAWAKELESQLGLNLPIYPIRGQICAYNIEPGRI
RHILYTSQGYLVPKANGTLVNGASEDIAGFQTEITDKGIRRLTNWNHKIVPFLAELTPFHKWAGLRPATQDGYPLIGFLR
NHPRVFMACGHYRNGILLSAITAKVASAQLAGQKPPVPIALFDPDRFS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=316, Percent_Identity=21.8354430379747, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71994045, Length=386, Percent_Identity=23.0569948186528, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71994052, Length=392, Percent_Identity=23.469387755102, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 40541; Mature: 40541

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHTIVLGGGVIGLSVAFQLAMDGKTVSVLEINTCGGQASGAAAGMLAPYSEISEDPDDF
CCCEEEECCCHHEEEHHEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHH
FRLCLASLRAFKEWQASVKQESGMTFEFTESGSLHCVYHEADLLSLATRKAWQEEFGAKV
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHCCEE
DVLTREELKKKEPELAEDIIGAIHYPEEAHVYAPDYVKALQQACRNRGVHIYEQLKHVSL
EEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCEEHHHHHHHHEE
VKTEPHIELQSASGQRFTADQLVVATGAWAKELESQLGLNLPIYPIRGQICAYNIEPGRI
EECCCCEEEECCCCCEEECCCEEEECCHHHHHHHHHHCCCCCEEEECCCEEEEECCCCEE
RHILYTSQGYLVPKANGTLVNGASEDIAGFQTEITDKGIRRLTNWNHKIVPFLAELTPFH
EEEEEECCCEEEECCCCCEECCCCHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHH
KWAGLRPATQDGYPLIGFLRNHPRVFMACGHYRNGILLSAITAKVASAQLAGQKPPVPIA
HHCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEEEHHHHHHHHHHHHCCCCCCEEEE
LFDPDRFS
EECCCCCC
>Mature Secondary Structure
MEHTIVLGGGVIGLSVAFQLAMDGKTVSVLEINTCGGQASGAAAGMLAPYSEISEDPDDF
CCCEEEECCCHHEEEHHEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHH
FRLCLASLRAFKEWQASVKQESGMTFEFTESGSLHCVYHEADLLSLATRKAWQEEFGAKV
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHCCEE
DVLTREELKKKEPELAEDIIGAIHYPEEAHVYAPDYVKALQQACRNRGVHIYEQLKHVSL
EEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCEEHHHHHHHHEE
VKTEPHIELQSASGQRFTADQLVVATGAWAKELESQLGLNLPIYPIRGQICAYNIEPGRI
EECCCCEEEECCCCCEEECCCEEEECCHHHHHHHHHHCCCCCEEEECCCEEEEECCCCEE
RHILYTSQGYLVPKANGTLVNGASEDIAGFQTEITDKGIRRLTNWNHKIVPFLAELTPFH
EEEEEECCCEEEECCCCCEECCCCHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHH
KWAGLRPATQDGYPLIGFLRNHPRVFMACGHYRNGILLSAITAKVASAQLAGQKPPVPIA
HHCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEEEHHHHHHHHHHHHCCCCCCEEEE
LFDPDRFS
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 12 {D-alpha-aminobutyrate}} 6 {D-phenylalanine}} 14 {D-glutamine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 19 {D-serine}} 0.15 {cytochrome} 29 {D-alanine}} 6.4

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]