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The map label for this gene is thiG

Identifier: 56963500

GI number: 56963500

Start: 1861944

End: 1862735

Strand: Direct

Name: thiG

Synonym: ABC1735

Alternate gene names: 56963500

Gene position: 1861944-1862735 (Clockwise)

Preceding gene: 56963499

Following gene: 56963501

Centisome position: 43.26

GC content: 47.47

Gene sequence:

>792_bases
ATGAAAAAAGTACCACTTGTCATTGGCGGAAAAACGCTCTCATCGCGGTTTTTCATTGGGACAGGCCGTTATCCAAACCC
GGTTGTTCAAAATGAAACCATTAAAGCCTCCGGAGCGGAGGTTTTAACATTTGCCATTCGCCGTATTAACCTAGAACATC
CTGATGAGGATGCAATTTTACAGCATTTAGAAGGGCGTACTTTTCAATACTTGCCAAATACATCAGGAGCGAACAATGCG
GAAGAGGCAATCCGTATTGCTCGTTTGGCCCGAGCGGCTGGGCTGAGCGATTGGATCAAAGTCGAAATTAGCGTATCTGA
AAAAACGCTATTGCCTGATCCGGTCGAGACATTAAAAGCGACAGAAGCTTTAGCGAAAGAAGGCTTCACTGTATTGCCGT
ATACATCAGATGACCCAATTCTTTGCAAAAAACTTGAGGAAGTCGGCGCAGCCGCAGTTATGCCTGGGGCTTCTCCAATT
GGCACAGGCCTTGGCATATTAAACCCGTATAACCTTGGCGTCATTGTCGAGGAAGCTTCTGTACCGATTATTGTGGATGC
TGGTCTCGGTTCTGCAAGCGATGTTGCCAAGGCAATGGAATTAGGGGCAGATGGAGTATTAATGAATACGGCAGTAGCTA
AAGCAAAGGATCCAGTAAAGATGGCGCTGGCAATGAAGCAAGCGATTGAAGCCGGTTTGCTGTCCTACGAAGCAGGGCGC
ATTCCGATGAAGCGCTATGCGACAGCAAGCAGCCAGCTTGACCATTTATTGTCCAGTCACAGTAAGGGGTAG

Upstream 100 bases:

>100_bases
AAGTCAACGGGACGATTATTGACCGTGTTGACTGGGAGGCCACTTCTTTGAGTGAAGGAATGAAAATCGAGCTAGTCCAT
TTCGTAGGGGGAGGATAAAC

Downstream 100 bases:

>100_bases
TCAAAATGGAGCATACAATTGTTTTAGGAGGCGGCGTTATCGGGCTGTCTGTTGCTTTTCAGTTAGCAATGGACGGCAAG
ACCGTTTCTGTCCTAGAAAT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKKVPLVIGGKTLSSRFFIGTGRYPNPVVQNETIKASGAEVLTFAIRRINLEHPDEDAILQHLEGRTFQYLPNTSGANNA
EEAIRIARLARAAGLSDWIKVEISVSEKTLLPDPVETLKATEALAKEGFTVLPYTSDDPILCKKLEEVGAAAVMPGASPI
GTGLGILNPYNLGVIVEEASVPIIVDAGLGSASDVAKAMELGADGVLMNTAVAKAKDPVKMALAMKQAIEAGLLSYEAGR
IPMKRYATASSQLDHLLSSHSKG

Sequences:

>Translated_263_residues
MKKVPLVIGGKTLSSRFFIGTGRYPNPVVQNETIKASGAEVLTFAIRRINLEHPDEDAILQHLEGRTFQYLPNTSGANNA
EEAIRIARLARAAGLSDWIKVEISVSEKTLLPDPVETLKATEALAKEGFTVLPYTSDDPILCKKLEEVGAAAVMPGASPI
GTGLGILNPYNLGVIVEEASVPIIVDAGLGSASDVAKAMELGADGVLMNTAVAKAKDPVKMALAMKQAIEAGLLSYEAGR
IPMKRYATASSQLDHLLSSHSKG
>Mature_263_residues
MKKVPLVIGGKTLSSRFFIGTGRYPNPVVQNETIKASGAEVLTFAIRRINLEHPDEDAILQHLEGRTFQYLPNTSGANNA
EEAIRIARLARAAGLSDWIKVEISVSEKTLLPDPVETLKATEALAKEGFTVLPYTSDDPILCKKLEEVGAAAVMPGASPI
GTGLGILNPYNLGVIVEEASVPIIVDAGLGSASDVAKAMELGADGVLMNTAVAKAKDPVKMALAMKQAIEAGLLSYEAGR
IPMKRYATASSQLDHLLSSHSKG

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=254, Percent_Identity=48.4251968503937, Blast_Score=236, Evalue=9e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_BACSK (Q5WH85)

Other databases:

- EMBL:   AP006627
- RefSeq:   YP_175231.1
- ProteinModelPortal:   Q5WH85
- SMR:   Q5WH85
- STRING:   Q5WH85
- EnsemblBacteria:   EBBACT00000048427
- GeneID:   3203414
- GenomeReviews:   AP006627_GR
- KEGG:   bcl:ABC1735
- NMPDR:   fig|66692.3.peg.1683
- eggNOG:   COG2022
- GeneTree:   EBGT00050000002572
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- BioCyc:   BCLA66692:ABC1735-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27817; Mature: 27817

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: ACT_SITE 100-100 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVPLVIGGKTLSSRFFIGTGRYPNPVVQNETIKASGAEVLTFAIRRINLEHPDEDAIL
CCCCCEEECCCEECCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHCCCCCCCHHHHH
QHLEGRTFQYLPNTSGANNAEEAIRIARLARAAGLSDWIKVEISVSEKTLLPDPVETLKA
HHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHH
TEALAKEGFTVLPYTSDDPILCKKLEEVGAAAVMPGASPIGTGLGILNPYNLGVIVEEAS
HHHHHHCCCEEEEECCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCC
VPIIVDAGLGSASDVAKAMELGADGVLMNTAVAKAKDPVKMALAMKQAIEAGLLSYEAGR
CCEEEECCCCCHHHHHHHHHCCCCCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
IPMKRYATASSQLDHLLSSHSKG
CCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKVPLVIGGKTLSSRFFIGTGRYPNPVVQNETIKASGAEVLTFAIRRINLEHPDEDAIL
CCCCCEEECCCEECCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHCCCCCCCHHHHH
QHLEGRTFQYLPNTSGANNAEEAIRIARLARAAGLSDWIKVEISVSEKTLLPDPVETLKA
HHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHH
TEALAKEGFTVLPYTSDDPILCKKLEEVGAAAVMPGASPIGTGLGILNPYNLGVIVEEAS
HHHHHHCCCEEEEECCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCC
VPIIVDAGLGSASDVAKAMELGADGVLMNTAVAKAKDPVKMALAMKQAIEAGLLSYEAGR
CCEEEECCCCCHHHHHHHHHCCCCCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
IPMKRYATASSQLDHLLSSHSKG
CCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA