Click here to switch to the map view.

The map label for this gene is nfo

Identifier: 56963463

GI number: 56963463

Start: 1826684

End: 1827562

Strand: Direct

Name: nfo

Synonym: ABC1698

Alternate gene names: 56963463

Gene position: 1826684-1827562 (Clockwise)

Preceding gene: 56963462

Following gene: 56963465

Centisome position: 42.44

GC content: 43.69

Gene sequence:

>879_bases
ATGTCATTGTTAATTGGTTCCCATGTGTCGATGAGCGGAAAGAAAATGTTGCTGCAATCGAGTGAGGAAGCAGCCTCTTA
CGGTGCAACGACATTCATGATTTATACAGGGGCGCCTCAAAATACACGGCGCAAAGCGATTGAGGATTTAAACATTGAAG
CGGGGAAAGCCCATATGGCGGAGCATGGCCTATCAAACATTGTCGTCCATGCTCCATATATCATCAATATTGGCAATACG
CAAAAGCCAGAAACCTTTCGCCTCGGCGTTGATTTTTTGCGATCTGAAATCGAACGCACTGAAGCGATAGGTGCAAAACA
GATTGTACTTCATCCAGGTGCTCATGTAGGTGCAGGGGCAGAGGAAGGCATTAAAAAGATCATTGAAGGTTTAAACGAAG
TTCTTACTGAAAAGCGCGATGTACAAATCGCTCTTGAGACGATGGCTGGGAAAGGGTCTGAGTGCGGCTGTACGTTTGAG
GAACTTGCTAAAATTATAGACGGCGTTACGCACAATGAGCGTTTATCGGTTTGTTTCGATACATGCCATACGCACGATGC
AGGCTATGATATTGTCAATGATTTTGACGGCGTGCTTGATGAATTTGACCGGATCATCGGAGCAGAACGGATTAAAGTCG
TCCATATCAATGATTCTAAAAATGAACGCAGTGCTCGGAAAGACCGCCATGAAAACATTGGCCATGGCCATATTGGCCTT
AAAGCGCTTGATTATATTGTCCATCATGACCAATTTAAAGACATTCCTAAAATTCTAGAAACGCCTTTCATAGGCAAAGA
CAAGAAAGATAAAAGGCCTCCATACAAACATGAAATTGCGTTGTTGCGCCGGGAGTTGCTAGAACCTATTGAAAAATAA

Upstream 100 bases:

>100_bases
CCTGGGTATAAACAAAAAGCGAAAGCCGCTATTGCCAAACAGAAAAAACGGGAGAGACGCTTAAACGCGCGTAAAGAGAA
ATAATACGGAGGGAGATTTC

Downstream 100 bases:

>100_bases
AGTTCCCAACACAAAGATTTTTTAATAGGCGGTGTGGCCAAAATGTTTCAAGCTCACGTTCTTATATAAGACGTGAGCTT
TTTGTGTTAATCCAAATAAT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSLLIGSHVSMSGKKMLLQSSEEAASYGATTFMIYTGAPQNTRRKAIEDLNIEAGKAHMAEHGLSNIVVHAPYIINIGNT
QKPETFRLGVDFLRSEIERTEAIGAKQIVLHPGAHVGAGAEEGIKKIIEGLNEVLTEKRDVQIALETMAGKGSECGCTFE
ELAKIIDGVTHNERLSVCFDTCHTHDAGYDIVNDFDGVLDEFDRIIGAERIKVVHINDSKNERSARKDRHENIGHGHIGL
KALDYIVHHDQFKDIPKILETPFIGKDKKDKRPPYKHEIALLRRELLEPIEK

Sequences:

>Translated_292_residues
MSLLIGSHVSMSGKKMLLQSSEEAASYGATTFMIYTGAPQNTRRKAIEDLNIEAGKAHMAEHGLSNIVVHAPYIINIGNT
QKPETFRLGVDFLRSEIERTEAIGAKQIVLHPGAHVGAGAEEGIKKIIEGLNEVLTEKRDVQIALETMAGKGSECGCTFE
ELAKIIDGVTHNERLSVCFDTCHTHDAGYDIVNDFDGVLDEFDRIIGAERIKVVHINDSKNERSARKDRHENIGHGHIGL
KALDYIVHHDQFKDIPKILETPFIGKDKKDKRPPYKHEIALLRRELLEPIEK
>Mature_291_residues
SLLIGSHVSMSGKKMLLQSSEEAASYGATTFMIYTGAPQNTRRKAIEDLNIEAGKAHMAEHGLSNIVVHAPYIINIGNTQ
KPETFRLGVDFLRSEIERTEAIGAKQIVLHPGAHVGAGAEEGIKKIIEGLNEVLTEKRDVQIALETMAGKGSECGCTFEE
LAKIIDGVTHNERLSVCFDTCHTHDAGYDIVNDFDGVLDEFDRIIGAERIKVVHINDSKNERSARKDRHENIGHGHIGLK
ALDYIVHHDQFKDIPKILETPFIGKDKKDKRPPYKHEIALLRRELLEPIEK

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=291, Percent_Identity=34.3642611683849, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17531193, Length=268, Percent_Identity=35.0746268656716, Blast_Score=150, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6322735, Length=289, Percent_Identity=30.4498269896194, Blast_Score=145, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_BACSK (Q5WHC2)

Other databases:

- EMBL:   AP006627
- RefSeq:   YP_175194.1
- ProteinModelPortal:   Q5WHC2
- SMR:   Q5WHC2
- STRING:   Q5WHC2
- EnsemblBacteria:   EBBACT00000046818
- GeneID:   3201116
- GenomeReviews:   AP006627_GR
- KEGG:   bcl:ABC1698
- NMPDR:   fig|66692.3.peg.1639
- eggNOG:   COG0648
- GeneTree:   EBGT00050000001754
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   PRK01060
- BioCyc:   BCLA66692:ABC1698-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 32501; Mature: 32369

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLIGSHVSMSGKKMLLQSSEEAASYGATTFMIYTGAPQNTRRKAIEDLNIEAGKAHMA
CCEEECCCCCCCCHHHHHHCCHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCHHHHH
EHGLSNIVVHAPYIINIGNTQKPETFRLGVDFLRSEIERTEAIGAKQIVLHPGAHVGAGA
HCCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCH
EEGIKKIIEGLNEVLTEKRDVQIALETMAGKGSECGCTFEELAKIIDGVTHNERLSVCFD
HHHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
TCHTHDAGYDIVNDFDGVLDEFDRIIGAERIKVVHINDSKNERSARKDRHENIGHGHIGL
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCHHH
KALDYIVHHDQFKDIPKILETPFIGKDKKDKRPPYKHEIALLRRELLEPIEK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLLIGSHVSMSGKKMLLQSSEEAASYGATTFMIYTGAPQNTRRKAIEDLNIEAGKAHMA
CEEECCCCCCCCHHHHHHCCHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCHHHHH
EHGLSNIVVHAPYIINIGNTQKPETFRLGVDFLRSEIERTEAIGAKQIVLHPGAHVGAGA
HCCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCH
EEGIKKIIEGLNEVLTEKRDVQIALETMAGKGSECGCTFEELAKIIDGVTHNERLSVCFD
HHHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
TCHTHDAGYDIVNDFDGVLDEFDRIIGAERIKVVHINDSKNERSARKDRHENIGHGHIGL
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCHHH
KALDYIVHHDQFKDIPKILETPFIGKDKKDKRPPYKHEIALLRRELLEPIEK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA