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The map label for this gene is kinE [H]

Identifier: 56963465

GI number: 56963465

Start: 1828088

End: 1829227

Strand: Direct

Name: kinE [H]

Synonym: ABC1700

Alternate gene names: 56963465

Gene position: 1828088-1829227 (Clockwise)

Preceding gene: 56963463

Following gene: 56963466

Centisome position: 42.48

GC content: 41.32

Gene sequence:

>1140_bases
ATGTGCAAGCTTTCCCAAAATCAAAAAGCAGCCCCTATAGGGAGTAACCAAGTGCGGCAAATGGCAAAAAGCGTAGATGT
ACACGATGACCATTTACTTTACATAAAAGAACCGCCTGACGTCGCACATTCAATGTTTTCTTTTATTAAAGCAATGGATA
TTCCTTACTTAAAGGTAGACGAGTGCTTTCGACAGCTCCACTGGAGCGAATCCTTTGTTCATCTCACCGATTTGGCAGAA
AAAGACCTTGAAGAACAAACACTGGCAGAGTTAGGCGTTTCTGATCATTTGCTGCATTTTATTAATGAACAATTAAGCAT
CTCAGTCGGGCGAGGCAACCAGATTGTTAAAAACTGTTATGAAGATGACGATGCGACAATCAAAGTGACTGTATTTCCTG
AGAGAGGGACGTATGCTTTTGTGTACTATGTGCTTTTAGAAGATTTGACGGTGAAACGGAAATTCGAGGAATTAGTCACA
TTTCAACATCAAATGCAAGCAGTATCGCATATTGCTGCCAGTGTCGCCCACGAGCTCCGGAACCCGTTGTCTGTCATTAA
GGGTTTTTTGCAATTATCCCATTTGACGAGCGATTTTCAGAAGTATTACAATACGATTATTTCAGAGCTAAACCGTATGA
ATGTCATTATTGAAGATTTTCTATCGGTTTCACGCAAAAAGAACGACCGTAAATGGCAATCGCCAGCACAGTTGCTCCAA
TCACTTGCTGAACTGATGCGGGCAGAATGCTCTCTGCACAGTGTTGAATTTCAACTTGACATTGAAGAAACGTTTGCGAT
ATGCCATGTAAACGAATCGATGTTTAAGCAAGTTATGCTTAATTTGTTGCGCAATTCAATTGAAGCTTATGAAGAGCAGT
CAACTGGACGGCGGCTGGAAATAAGAAGCAGGGAAGTTGGCGAGCAAGTCGTTATTGAGCTGATCGATAATGGCAAAGGC
ATGCCTGATACTGTTTTAAGCCAACTTGGAAAACCATTTTTTACGACAAAAGAAAAAGGCACGGGCGTCGGTATCCCGTT
ATGCAAAAAGATTATTGAAGACCATGGAGGCACATTTGATATTCAGAGTGAAGCCAATGTTGGGACGCGAATTGAGATGA
TATTGCCATTATTAGTTTGA

Upstream 100 bases:

>100_bases
AGGAAAAAACGCCATTATCTCGAATAAGAATAGAGTAGCGGGGAATGTTTACGGCCATGGTTGTTAGATAAGGATCACTA
CTGAAGAAGGAGGGGCAAGG

Downstream 100 bases:

>100_bases
AAAGGATATTTATTGGTTTTGTTCCAATCATTTTTCGAGCCTGAATGAGCCTTTTATTGGCGCGGATCAAGTGAAATAAG
GCAGCAATTCGCCCTTTGAG

Product: stage II sporulation protein J, sporulation kinase A

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MCKLSQNQKAAPIGSNQVRQMAKSVDVHDDHLLYIKEPPDVAHSMFSFIKAMDIPYLKVDECFRQLHWSESFVHLTDLAE
KDLEEQTLAELGVSDHLLHFINEQLSISVGRGNQIVKNCYEDDDATIKVTVFPERGTYAFVYYVLLEDLTVKRKFEELVT
FQHQMQAVSHIAASVAHELRNPLSVIKGFLQLSHLTSDFQKYYNTIISELNRMNVIIEDFLSVSRKKNDRKWQSPAQLLQ
SLAELMRAECSLHSVEFQLDIEETFAICHVNESMFKQVMLNLLRNSIEAYEEQSTGRRLEIRSREVGEQVVIELIDNGKG
MPDTVLSQLGKPFFTTKEKGTGVGIPLCKKIIEDHGGTFDIQSEANVGTRIEMILPLLV

Sequences:

>Translated_379_residues
MCKLSQNQKAAPIGSNQVRQMAKSVDVHDDHLLYIKEPPDVAHSMFSFIKAMDIPYLKVDECFRQLHWSESFVHLTDLAE
KDLEEQTLAELGVSDHLLHFINEQLSISVGRGNQIVKNCYEDDDATIKVTVFPERGTYAFVYYVLLEDLTVKRKFEELVT
FQHQMQAVSHIAASVAHELRNPLSVIKGFLQLSHLTSDFQKYYNTIISELNRMNVIIEDFLSVSRKKNDRKWQSPAQLLQ
SLAELMRAECSLHSVEFQLDIEETFAICHVNESMFKQVMLNLLRNSIEAYEEQSTGRRLEIRSREVGEQVVIELIDNGKG
MPDTVLSQLGKPFFTTKEKGTGVGIPLCKKIIEDHGGTFDIQSEANVGTRIEMILPLLV
>Mature_379_residues
MCKLSQNQKAAPIGSNQVRQMAKSVDVHDDHLLYIKEPPDVAHSMFSFIKAMDIPYLKVDECFRQLHWSESFVHLTDLAE
KDLEEQTLAELGVSDHLLHFINEQLSISVGRGNQIVKNCYEDDDATIKVTVFPERGTYAFVYYVLLEDLTVKRKFEELVT
FQHQMQAVSHIAASVAHELRNPLSVIKGFLQLSHLTSDFQKYYNTIISELNRMNVIIEDFLSVSRKKNDRKWQSPAQLLQ
SLAELMRAECSLHSVEFQLDIEETFAICHVNESMFKQVMLNLLRNSIEAYEEQSTGRRLEIRSREVGEQVVIELIDNGKG
MPDTVLSQLGKPFFTTKEKGTGVGIPLCKKIIEDHGGTFDIQSEANVGTRIEMILPLLV

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=240, Percent_Identity=29.1666666666667, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1790436, Length=239, Percent_Identity=29.7071129707113, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1786912, Length=254, Percent_Identity=22.8346456692913, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790300, Length=220, Percent_Identity=25.4545454545455, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081816, Length=228, Percent_Identity=27.1929824561404, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI145693157, Length=265, Percent_Identity=26.4150943396226, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786600, Length=238, Percent_Identity=23.109243697479, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789149, Length=217, Percent_Identity=25.8064516129032, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI48994928, Length=239, Percent_Identity=21.7573221757322, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788713, Length=228, Percent_Identity=25, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 43247; Mature: 43247

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKLSQNQKAAPIGSNQVRQMAKSVDVHDDHLLYIKEPPDVAHSMFSFIKAMDIPYLKVD
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCHHH
ECFRQLHWSESFVHLTDLAEKDLEEQTLAELGVSDHLLHFINEQLSISVGRGNQIVKNCY
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEECCCHHHHHHHH
EDDDATIKVTVFPERGTYAFVYYVLLEDLTVKRKFEELVTFQHQMQAVSHIAASVAHELR
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NPLSVIKGFLQLSHLTSDFQKYYNTIISELNRMNVIIEDFLSVSRKKNDRKWQSPAQLLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
SLAELMRAECSLHSVEFQLDIEETFAICHVNESMFKQVMLNLLRNSIEAYEEQSTGRRLE
HHHHHHHHHHHHHHEEEEEEHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IRSREVGEQVVIELIDNGKGMPDTVLSQLGKPFFTTKEKGTGVGIPLCKKIIEDHGGTFD
EHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEE
IQSEANVGTRIEMILPLLV
ECCCCCCCHHHHHHHHHHC
>Mature Secondary Structure
MCKLSQNQKAAPIGSNQVRQMAKSVDVHDDHLLYIKEPPDVAHSMFSFIKAMDIPYLKVD
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCHHH
ECFRQLHWSESFVHLTDLAEKDLEEQTLAELGVSDHLLHFINEQLSISVGRGNQIVKNCY
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEECCCHHHHHHHH
EDDDATIKVTVFPERGTYAFVYYVLLEDLTVKRKFEELVTFQHQMQAVSHIAASVAHELR
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NPLSVIKGFLQLSHLTSDFQKYYNTIISELNRMNVIIEDFLSVSRKKNDRKWQSPAQLLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
SLAELMRAECSLHSVEFQLDIEETFAICHVNESMFKQVMLNLLRNSIEAYEEQSTGRRLE
HHHHHHHHHHHHHHEEEEEEHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IRSREVGEQVVIELIDNGKGMPDTVLSQLGKPFFTTKEKGTGVGIPLCKKIIEDHGGTFD
EHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEE
IQSEANVGTRIEMILPLLV
ECCCCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]