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The map label for this gene is nasB [H]

Identifier: 56963388

GI number: 56963388

Start: 1751168

End: 1753396

Strand: Direct

Name: nasB [H]

Synonym: ABC1623

Alternate gene names: 56963388

Gene position: 1751168-1753396 (Clockwise)

Preceding gene: 56963387

Following gene: 56963389

Centisome position: 40.69

GC content: 48.54

Gene sequence:

>2229_bases
ATGGGCTCGGAGTCAAGACAGCGCCTCGTCCTTATTGGCAATGGCATGGCAGGCGTCCGCGTGGTAGAGGAAGTGTTAAA
ACTTGCTCCGCGCCAATTTGCGATTACGATTCTATCAGAAGAAGACCGCCCGAGCTATAACCGCATCTTGCTTTCCTCCG
TTTTGCAAGGGAAACAGACGCTTCACAGCATTGGTATCCATCCGTTCCAATGGTATGTTGACCATGGCATTGAGCTGCGA
TTAGGTGAGGAAGCGACCAGAATCGATCCCGTTGCCCAGAAAGTATATACCGACAAAGGGGAACTTGCTTACGACAAGCT
AGTGATCGCTACTGGTTCGGAACCTTTTGTCCTTCCAGTGCCAGGAGTGGAGAAAGAAGGCGTATTTGTTTTTCGCACAA
TCCGTGATTGTGAACGGTTAACTGAATGGTCGACTCGTTACCAAAAAGCGGCTGTCATTGGGGGCGGTTTGCTTGGTCTT
GAAGCCGCCCAAGGGCTGGCTCATTTAGGCGTCGACGTAAGCGTCATTCATCTTTCTGACCGAATTATGGAGCGCCAGCT
CGACGAAACGGCAGCCCGGCTGTTGAAACAGAGCCTTGAGAGCAAAGGGATTCACTTTTTGTTACAAAAACAAACAGCGG
AAATTAGCGGCGAGGAGCGGGTAACAGGTCTTCTTTTTCATGATGGAACGACTCTTTCTGCTGATTTTGTTGTCATGGCA
GCTGGCGTCCAGCCGAACATTGCCATTGCCAAGGAAAGCAACATTCCAACAAAGCGAGGAATTGTAGTTGATGATCGTCT
ACGCACGGACGTTTCAGGTGTGTATGCCGTCGGCGAATGTGCCGAACATAACGGAATCAGCTATGGGTTAGTGGCCCCCT
TATATGAACAAGCAAAAGTGCTGGCCCGTGAGCTTTGTGGACTGCCAGGCCTGTATTATAATGGCACTGTGTTAGCGACG
CAATTGAAAATATCAGGAGTAAACGTCTTTTCTGCAGGCGAAACAACCTGTTCTGGCACTTGTACTGTTTTGAAAACTTT
AAACGAAATTACGGGTCAGTACAAGAAGCTTCTGTTTAAAGAGGATTGCCTTGCCGGGGCAGTATTGTTTGGCAATACGC
GACAAAGCGACCAGCTGTTGCAGTTGATTCGGGCAAACACGCCATTGTCAGATGACGAGAAGACGCAACTATTAACAGAA
GATGGAACAGCATTTGAACGAGAGCTAGCCTCTCTGCCAGCTTCCCATCTCATTTGCAATTGTAATGCTGTATCAAAGGG
CGACATTATGAGCGCAATGGCAGCCGGACAGCACACAATTGCACAGATAAAAGCGGGAACGAAAGCGGCAAGTTCATGCG
GTGGATGCGAGGTCAGAGTGGCCGCTATGCTAGAAAGCTTTAAGCATGGACAGTTGCAAGCGACTGTCGATATAGCCGAA
AGAAAACCGTTGTGCCCATGCACGTCGCTAACCGAACAACAAGTCGCCCAATCAATCGCCGAGAAACGGCTCGTTGTGTT
TTCTGACATGAGCGAAAAGCTAGGTTGGAAAAACAAAAATGGGTGCCGAGTCTGCCGAGAAGTGTCTGCTTATTATGCCG
GATTGTTCTTTCCTGGGGGAGCACCAAAGGAACGGAATGGTAAACAAGAAAACGATGGCAGTTATACGATTACACTTCCA
CACATTGCTGGTAGATTGAGCACTGACCAATTAAAACAATTTGCCCTAATGGCTGAGGAACTGCAAGTGCCGAGAATTGA
TTTTTCAGCACGCAACCAAGTGCAGCTGAAAGGGTTGACGGAAAAGCAACTGCCTTACGTCTTTGAACAGCTTGATATGC
CTCCTGCTGTTTTGGCAGGCAAGCGTTTGCAGGTTGCTATTGATGACAGCCACTATAACTGGCCGGAAGGGAGGCACTTG
GCTGAAAGACTGCTCGATTTATTTAACTGTGTGCCTTTTCCTGCGGAAACAGAAGTATGCGTTACGTTAGCTGCTCGTCG
ACACGAGCTCGCTGCTATTCGTCTCTCTCGGTCGCCATTAAATTGGGAAGTAGCGATTGGTTCTGAATTATTTTGTTCGA
ACGACTCCGTTGAAGGAACCTTTCAACTTGTTTGCGCTTTGCTGCAATACTTTAGGCAATCAGCGCAGTACAGTGAAAAT
ATCCATGCTTGGCTTGTCCGTAAAGGCGCGGTCCATGTTCGCGAAGTCGTGTTTGACGCTGCTTGCTAG

Upstream 100 bases:

>100_bases
CGCAAGGTGGAGACCAAGGAAATGTAGCTTGTTTTACTGTGAAAGTTGAAGGAGATAATATTTACGTGTTTATTTAATGC
TTGTTAAAAGGAGTGATTGG

Downstream 100 bases:

>100_bases
GCAAAATTAAATAAACAGCTAGCGGAAATAACGGCAGTTAGCCATGAGCATGCGGAACGAATGCCAGTCGTTTAAAGAGA
TAAAAATTTGGAGTGGAGGG

Product: assimilatory nitrate reductase electron transfer subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 742; Mature: 741

Protein sequence:

>742_residues
MGSESRQRLVLIGNGMAGVRVVEEVLKLAPRQFAITILSEEDRPSYNRILLSSVLQGKQTLHSIGIHPFQWYVDHGIELR
LGEEATRIDPVAQKVYTDKGELAYDKLVIATGSEPFVLPVPGVEKEGVFVFRTIRDCERLTEWSTRYQKAAVIGGGLLGL
EAAQGLAHLGVDVSVIHLSDRIMERQLDETAARLLKQSLESKGIHFLLQKQTAEISGEERVTGLLFHDGTTLSADFVVMA
AGVQPNIAIAKESNIPTKRGIVVDDRLRTDVSGVYAVGECAEHNGISYGLVAPLYEQAKVLARELCGLPGLYYNGTVLAT
QLKISGVNVFSAGETTCSGTCTVLKTLNEITGQYKKLLFKEDCLAGAVLFGNTRQSDQLLQLIRANTPLSDDEKTQLLTE
DGTAFERELASLPASHLICNCNAVSKGDIMSAMAAGQHTIAQIKAGTKAASSCGGCEVRVAAMLESFKHGQLQATVDIAE
RKPLCPCTSLTEQQVAQSIAEKRLVVFSDMSEKLGWKNKNGCRVCREVSAYYAGLFFPGGAPKERNGKQENDGSYTITLP
HIAGRLSTDQLKQFALMAEELQVPRIDFSARNQVQLKGLTEKQLPYVFEQLDMPPAVLAGKRLQVAIDDSHYNWPEGRHL
AERLLDLFNCVPFPAETEVCVTLAARRHELAAIRLSRSPLNWEVAIGSELFCSNDSVEGTFQLVCALLQYFRQSAQYSEN
IHAWLVRKGAVHVREVVFDAAC

Sequences:

>Translated_742_residues
MGSESRQRLVLIGNGMAGVRVVEEVLKLAPRQFAITILSEEDRPSYNRILLSSVLQGKQTLHSIGIHPFQWYVDHGIELR
LGEEATRIDPVAQKVYTDKGELAYDKLVIATGSEPFVLPVPGVEKEGVFVFRTIRDCERLTEWSTRYQKAAVIGGGLLGL
EAAQGLAHLGVDVSVIHLSDRIMERQLDETAARLLKQSLESKGIHFLLQKQTAEISGEERVTGLLFHDGTTLSADFVVMA
AGVQPNIAIAKESNIPTKRGIVVDDRLRTDVSGVYAVGECAEHNGISYGLVAPLYEQAKVLARELCGLPGLYYNGTVLAT
QLKISGVNVFSAGETTCSGTCTVLKTLNEITGQYKKLLFKEDCLAGAVLFGNTRQSDQLLQLIRANTPLSDDEKTQLLTE
DGTAFERELASLPASHLICNCNAVSKGDIMSAMAAGQHTIAQIKAGTKAASSCGGCEVRVAAMLESFKHGQLQATVDIAE
RKPLCPCTSLTEQQVAQSIAEKRLVVFSDMSEKLGWKNKNGCRVCREVSAYYAGLFFPGGAPKERNGKQENDGSYTITLP
HIAGRLSTDQLKQFALMAEELQVPRIDFSARNQVQLKGLTEKQLPYVFEQLDMPPAVLAGKRLQVAIDDSHYNWPEGRHL
AERLLDLFNCVPFPAETEVCVTLAARRHELAAIRLSRSPLNWEVAIGSELFCSNDSVEGTFQLVCALLQYFRQSAQYSEN
IHAWLVRKGAVHVREVVFDAAC
>Mature_741_residues
GSESRQRLVLIGNGMAGVRVVEEVLKLAPRQFAITILSEEDRPSYNRILLSSVLQGKQTLHSIGIHPFQWYVDHGIELRL
GEEATRIDPVAQKVYTDKGELAYDKLVIATGSEPFVLPVPGVEKEGVFVFRTIRDCERLTEWSTRYQKAAVIGGGLLGLE
AAQGLAHLGVDVSVIHLSDRIMERQLDETAARLLKQSLESKGIHFLLQKQTAEISGEERVTGLLFHDGTTLSADFVVMAA
GVQPNIAIAKESNIPTKRGIVVDDRLRTDVSGVYAVGECAEHNGISYGLVAPLYEQAKVLARELCGLPGLYYNGTVLATQ
LKISGVNVFSAGETTCSGTCTVLKTLNEITGQYKKLLFKEDCLAGAVLFGNTRQSDQLLQLIRANTPLSDDEKTQLLTED
GTAFERELASLPASHLICNCNAVSKGDIMSAMAAGQHTIAQIKAGTKAASSCGGCEVRVAAMLESFKHGQLQATVDIAER
KPLCPCTSLTEQQVAQSIAEKRLVVFSDMSEKLGWKNKNGCRVCREVSAYYAGLFFPGGAPKERNGKQENDGSYTITLPH
IAGRLSTDQLKQFALMAEELQVPRIDFSARNQVQLKGLTEKQLPYVFEQLDMPPAVLAGKRLQVAIDDSHYNWPEGRHLA
ERLLDLFNCVPFPAETEVCVTLAARRHELAAIRLSRSPLNWEVAIGSELFCSNDSVEGTFQLVCALLQYFRQSAQYSENI
HAWLVRKGAVHVREVVFDAAC

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=282, Percent_Identity=28.7234042553192, Blast_Score=109, Evalue=1e-23,
Organism=Homo sapiens, GI226437568, Length=282, Percent_Identity=28.7234042553192, Blast_Score=109, Evalue=1e-23,
Organism=Homo sapiens, GI65787454, Length=282, Percent_Identity=28.7234042553192, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1789765, Length=634, Percent_Identity=31.5457413249211, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1789065, Length=303, Percent_Identity=28.0528052805281, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1788892, Length=248, Percent_Identity=28.2258064516129, Blast_Score=96, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=395, Percent_Identity=22.5316455696203, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281359715, Length=320, Percent_Identity=24.0625, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281359713, Length=320, Percent_Identity=24.0625, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24639250, Length=320, Percent_Identity=24.0625, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18543267, Length=320, Percent_Identity=24.0625, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24639252, Length=320, Percent_Identity=24.0625, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24639257, Length=320, Percent_Identity=24.0625, Blast_Score=92, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585130, Length=284, Percent_Identity=23.943661971831, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI28573993, Length=311, Percent_Identity=24.7588424437299, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24581020, Length=311, Percent_Identity=24.7588424437299, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 81335; Mature: 81204

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSESRQRLVLIGNGMAGVRVVEEVLKLAPRQFAITILSEEDRPSYNRILLSSVLQGKQT
CCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHH
LHSIGIHPFQWYVDHGIELRLGEEATRIDPVAQKVYTDKGELAYDKLVIATGSEPFVLPV
HHHCCCCCEEEEECCCEEEEECCCHHHCCHHHHHHHCCCCCEEEEEEEEEECCCCEEEEC
PGVEKEGVFVFRTIRDCERLTEWSTRYQKAAVIGGGLLGLEAAQGLAHLGVDVSVIHLSD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCCHHHCCCCEEEEEHHH
RIMERQLDETAARLLKQSLESKGIHFLLQKQTAEISGEERVTGLLFHDGTTLSADFVVMA
HHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHCCCCCCCEEEEEEECCCCCCCCEEEEE
AGVQPNIAIAKESNIPTKRGIVVDDRLRTDVSGVYAVGECAEHNGISYGLVAPLYEQAKV
ECCCCCEEEEECCCCCCCCCEEECCHHHCCCCHHEEEHHHHHCCCCCCCHHHHHHHHHHH
LARELCGLPGLYYNGTVLATQLKISGVNVFSAGETTCSGTCTVLKTLNEITGQYKKLLFK
HHHHHHCCCCCEECCEEEEEEEEEECEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EDCLAGAVLFGNTRQSDQLLQLIRANTPLSDDEKTQLLTEDGTAFERELASLPASHLICN
HHHHEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHEEEECCCCHHHHHHHHCCHHHEEEE
CNAVSKGDIMSAMAAGQHTIAQIKAGTKAASSCGGCEVRVAAMLESFKHGQLQATVDIAE
CCCCCCCHHHHHHHCCCHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCCCCEEEEEEHHC
RKPLCPCTSLTEQQVAQSIAEKRLVVFSDMSEKLGWKNKNGCRVCREVSAYYAGLFFPGG
CCCCCCCCCCHHHHHHHHHHHCEEEEEECCHHHCCCCCCCHHHHHHHHHHHHCEEECCCC
APKERNGKQENDGSYTITLPHIAGRLSTDQLKQFALMAEELQVPRIDFSARNQVQLKGLT
CCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC
EKQLPYVFEQLDMPPAVLAGKRLQVAIDDSHYNWPEGRHLAERLLDLFNCVPFPAETEVC
HHHHHHHHHHCCCCHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
VTLAARRHELAAIRLSRSPLNWEVAIGSELFCSNDSVEGTFQLVCALLQYFRQSAQYSEN
HHHHHHHHHEEEEEEECCCCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
IHAWLVRKGAVHVREVVFDAAC
CEEEEEECCCHHHHHHHHHCCC
>Mature Secondary Structure 
GSESRQRLVLIGNGMAGVRVVEEVLKLAPRQFAITILSEEDRPSYNRILLSSVLQGKQT
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHH
LHSIGIHPFQWYVDHGIELRLGEEATRIDPVAQKVYTDKGELAYDKLVIATGSEPFVLPV
HHHCCCCCEEEEECCCEEEEECCCHHHCCHHHHHHHCCCCCEEEEEEEEEECCCCEEEEC
PGVEKEGVFVFRTIRDCERLTEWSTRYQKAAVIGGGLLGLEAAQGLAHLGVDVSVIHLSD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCCHHHCCCCEEEEEHHH
RIMERQLDETAARLLKQSLESKGIHFLLQKQTAEISGEERVTGLLFHDGTTLSADFVVMA
HHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHCCCCCCCEEEEEEECCCCCCCCEEEEE
AGVQPNIAIAKESNIPTKRGIVVDDRLRTDVSGVYAVGECAEHNGISYGLVAPLYEQAKV
ECCCCCEEEEECCCCCCCCCEEECCHHHCCCCHHEEEHHHHHCCCCCCCHHHHHHHHHHH
LARELCGLPGLYYNGTVLATQLKISGVNVFSAGETTCSGTCTVLKTLNEITGQYKKLLFK
HHHHHHCCCCCEECCEEEEEEEEEECEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EDCLAGAVLFGNTRQSDQLLQLIRANTPLSDDEKTQLLTEDGTAFERELASLPASHLICN
HHHHEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHEEEECCCCHHHHHHHHCCHHHEEEE
CNAVSKGDIMSAMAAGQHTIAQIKAGTKAASSCGGCEVRVAAMLESFKHGQLQATVDIAE
CCCCCCCHHHHHHHCCCHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCCCCEEEEEEHHC
RKPLCPCTSLTEQQVAQSIAEKRLVVFSDMSEKLGWKNKNGCRVCREVSAYYAGLFFPGG
CCCCCCCCCCHHHHHHHHHHHCEEEEEECCHHHCCCCCCCHHHHHHHHHHHHCEEECCCC
APKERNGKQENDGSYTITLPHIAGRLSTDQLKQFALMAEELQVPRIDFSARNQVQLKGLT
CCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC
EKQLPYVFEQLDMPPAVLAGKRLQVAIDDSHYNWPEGRHLAERLLDLFNCVPFPAETEVC
HHHHHHHHHHCCCCHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
VTLAARRHELAAIRLSRSPLNWEVAIGSELFCSNDSVEGTFQLVCALLQYFRQSAQYSEN
HHHHHHHHHEEEEEEECCCCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
IHAWLVRKGAVHVREVVFDAAC
CEEEEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]