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The map label for this gene is nasC [H]

Identifier: 56963389

GI number: 56963389

Start: 1753508

End: 1755667

Strand: Direct

Name: nasC [H]

Synonym: ABC1624

Alternate gene names: 56963389

Gene position: 1753508-1755667 (Clockwise)

Preceding gene: 56963388

Following gene: 56963390

Centisome position: 40.74

GC content: 45.42

Gene sequence:

>2160_bases
ATGCAGTCGGGATATTTGCAAGAAAAAAGACAACCGCCTAATCGTAAAATCGTCTATAACAGCCAATGCCCTTTTTGCAG
TGTACAATGCAAGATGCAATTGATTGAGCGTAAGCATGGACAGGAAACATCATACGACGTTGTTGGCATAGAGAACCCCA
CGTCAAAAGGCCGTTTATGTGTAAAAGGGCTGAATGCTCACCAACACGCCTTTCATCCCGATCGGCTTACACAACCGCTG
GCCAAAGTAAATGGAAAATGGCTGCCGATTAGTTGGGATGAAGCACTGAAACAAATCGCCGAAAACGTTCAAGCAATTCA
GCAAGAAGATGGGCATGATGCAATGGCCGTATATGGAAGCGCCTCAATTACAAATGAAGAAGCATATTTGCTCGGTAAGT
TTGCCCGTGTAGCCTTGAAAACAAGGCATATTGACTATAATGGCCGGCTCTGTATGTCGGCTGCTGCCTCCGCTGCTACA
ACAGCATTTGGAATAGATAGAGGCTTAACCAATTCCCTTGAAGACGTTCTGTTTGCGGAATGTCTGATGTTGGTAGGGAC
CAATTTAGCTGAATGCCAACCAACGCTGATGCCTTACATTGAACAAGCGAAAAAGAACGGCGCTTTTCTTATCGTCGTTG
ATCCAAGGGAAACGGCAACTGCACAGCTGGCTGACTTGCATATAAAGCTAAAACCTGGTACAGACGCTGCTTTAGCAAAT
GGGTTATTGAAATGTATCTTAGAAGGTGGCTACGAAGATGCCGCTTTCATCCAAAAACGGGTTGAAGGGTTTGCGGAAGT
CAAAGCCCATATTGCCAAGCTCAAACTTGAAAACATAGCCGAGCAAACAGGGGTCAGCGGCGATGTGATCACCAAGGCTG
CTGAAACGTTTGCTAAGGCAAGAACAGGGATGATTTTTACGGCAAGGGGCGTTGAGCAGCAAACAAACGGCTCCATGGCT
GTCCGTAATTTCCTGAATGTTCTAATCGCTACAGGGAAGTTAGGCAAACACTCATGTGGATTTGCCGCGGTTACAGGACA
AAGTAATGGCCAAGGGGCAAGGGAACATGGTCAAAAAGCCGATCAGCTTCCAGGATACCGTTCAATCGAAAATGAAGAGC
ATCGCGCTCATATTGCCGCTATTTGGGGGATTTCACCTGAACAGTTGCCAAGGAAGGGTGTCTCGGCTTTTGAAATGATG
AAAAAAGCAGACAAGGGGGAAATTACCGGAATGTTTTTAATGTGCTCCAATCCGGTCGTATCCCATCCACAGGCCGATTT
TGTCAAAAATGCTTTAAAAAAGCTGAAATTTCTGGTCGCTGTCGACTTATTTGTTTCTGAAACAGCTCAGCTTGCTGACT
TGATCTTGCCGTCCACTTCTTATTTGGAAGATGAAGGCACGATGACAAATGTCGAAGGCAGAGTGACACTGCGTGAAGCC
AGTATGCCTCCTTATGGGGAAGCCCGCCATGATTGGCAAATTATCTGTAACATTGCGGCTGTTCTTGGCAAAGGAACTGA
TTTTTCCTATTCCTGCGCGGAAGACATTTTTACTGAATTAAGAATGGCCAGCAAAGGTGGAATCGCTGATTACTATGGAA
TTACGTATGAGCGGCTTAGGAAAGAAAAGGGGATTTTTTGGCCATGCCCTAGCCTAGAACATCCAGGGACGCCACTGTTA
TTTACGGATTGTTTTTATCGGCCAAACAAAAAAGCGCTTATGACCGTTGTTCCGAACGACGCGCCTGTACCAAAAGAAAA
ACCAAGTCGAGATTACCCTCTTTATTTAACGACGGGCAGAGTGCTTGCTCACTATTTGACTGGCGTACAGACACGAAATA
GCGCCTCCCTTATGGCACGAAACTTTGAATCTTTTGTAGAAATGCATCCAGAAACCGCTAAAAAGCATGGAATTGCTGAT
CGAACGCTTATCAAGCTGACTACCAAACAAGGCAGTATGATCGTCCGTTGTCACTTTACGGAAACGATTCGTGAAGATAC
TGTATTTGTCCCTTTTCACTGGAGTGGCAAACAAAACGTCAATCGTATAATTACAGGCAATTTAGACCCTGATTGCCGCA
TGCCAGCTTTTAAAGTCAGCGCAGTAAGGGCTGAACCATTAGTTGTTCATGAGCAAATGGAAGGAGTGTTGGGAACATGA

Upstream 100 bases:

>100_bases
TAAACAGCTAGCGGAAATAACGGCAGTTAGCCATGAGCATGCGGAACGAATGCCAGTCGTTTAAAGAGATAAAAATTTGG
AGTGGAGGGATTGTACTCGA

Downstream 100 bases:

>100_bases
AACTTTCTGAGTTACGCAAAAGCGGGCATGCGCCTTCGTTGTTTTCCGCTTTTTTATATTTCGATGTAAGCTTTATGATT
TGGGTATTGCTTGGTGCCCT

Product: assimilatory nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 719; Mature: 719

Protein sequence:

>719_residues
MQSGYLQEKRQPPNRKIVYNSQCPFCSVQCKMQLIERKHGQETSYDVVGIENPTSKGRLCVKGLNAHQHAFHPDRLTQPL
AKVNGKWLPISWDEALKQIAENVQAIQQEDGHDAMAVYGSASITNEEAYLLGKFARVALKTRHIDYNGRLCMSAAASAAT
TAFGIDRGLTNSLEDVLFAECLMLVGTNLAECQPTLMPYIEQAKKNGAFLIVVDPRETATAQLADLHIKLKPGTDAALAN
GLLKCILEGGYEDAAFIQKRVEGFAEVKAHIAKLKLENIAEQTGVSGDVITKAAETFAKARTGMIFTARGVEQQTNGSMA
VRNFLNVLIATGKLGKHSCGFAAVTGQSNGQGAREHGQKADQLPGYRSIENEEHRAHIAAIWGISPEQLPRKGVSAFEMM
KKADKGEITGMFLMCSNPVVSHPQADFVKNALKKLKFLVAVDLFVSETAQLADLILPSTSYLEDEGTMTNVEGRVTLREA
SMPPYGEARHDWQIICNIAAVLGKGTDFSYSCAEDIFTELRMASKGGIADYYGITYERLRKEKGIFWPCPSLEHPGTPLL
FTDCFYRPNKKALMTVVPNDAPVPKEKPSRDYPLYLTTGRVLAHYLTGVQTRNSASLMARNFESFVEMHPETAKKHGIAD
RTLIKLTTKQGSMIVRCHFTETIREDTVFVPFHWSGKQNVNRIITGNLDPDCRMPAFKVSAVRAEPLVVHEQMEGVLGT

Sequences:

>Translated_719_residues
MQSGYLQEKRQPPNRKIVYNSQCPFCSVQCKMQLIERKHGQETSYDVVGIENPTSKGRLCVKGLNAHQHAFHPDRLTQPL
AKVNGKWLPISWDEALKQIAENVQAIQQEDGHDAMAVYGSASITNEEAYLLGKFARVALKTRHIDYNGRLCMSAAASAAT
TAFGIDRGLTNSLEDVLFAECLMLVGTNLAECQPTLMPYIEQAKKNGAFLIVVDPRETATAQLADLHIKLKPGTDAALAN
GLLKCILEGGYEDAAFIQKRVEGFAEVKAHIAKLKLENIAEQTGVSGDVITKAAETFAKARTGMIFTARGVEQQTNGSMA
VRNFLNVLIATGKLGKHSCGFAAVTGQSNGQGAREHGQKADQLPGYRSIENEEHRAHIAAIWGISPEQLPRKGVSAFEMM
KKADKGEITGMFLMCSNPVVSHPQADFVKNALKKLKFLVAVDLFVSETAQLADLILPSTSYLEDEGTMTNVEGRVTLREA
SMPPYGEARHDWQIICNIAAVLGKGTDFSYSCAEDIFTELRMASKGGIADYYGITYERLRKEKGIFWPCPSLEHPGTPLL
FTDCFYRPNKKALMTVVPNDAPVPKEKPSRDYPLYLTTGRVLAHYLTGVQTRNSASLMARNFESFVEMHPETAKKHGIAD
RTLIKLTTKQGSMIVRCHFTETIREDTVFVPFHWSGKQNVNRIITGNLDPDCRMPAFKVSAVRAEPLVVHEQMEGVLGT
>Mature_719_residues
MQSGYLQEKRQPPNRKIVYNSQCPFCSVQCKMQLIERKHGQETSYDVVGIENPTSKGRLCVKGLNAHQHAFHPDRLTQPL
AKVNGKWLPISWDEALKQIAENVQAIQQEDGHDAMAVYGSASITNEEAYLLGKFARVALKTRHIDYNGRLCMSAAASAAT
TAFGIDRGLTNSLEDVLFAECLMLVGTNLAECQPTLMPYIEQAKKNGAFLIVVDPRETATAQLADLHIKLKPGTDAALAN
GLLKCILEGGYEDAAFIQKRVEGFAEVKAHIAKLKLENIAEQTGVSGDVITKAAETFAKARTGMIFTARGVEQQTNGSMA
VRNFLNVLIATGKLGKHSCGFAAVTGQSNGQGAREHGQKADQLPGYRSIENEEHRAHIAAIWGISPEQLPRKGVSAFEMM
KKADKGEITGMFLMCSNPVVSHPQADFVKNALKKLKFLVAVDLFVSETAQLADLILPSTSYLEDEGTMTNVEGRVTLREA
SMPPYGEARHDWQIICNIAAVLGKGTDFSYSCAEDIFTELRMASKGGIADYYGITYERLRKEKGIFWPCPSLEHPGTPLL
FTDCFYRPNKKALMTVVPNDAPVPKEKPSRDYPLYLTTGRVLAHYLTGVQTRNSASLMARNFESFVEMHPETAKKHGIAD
RTLIKLTTKQGSMIVRCHFTETIREDTVFVPFHWSGKQNVNRIITGNLDPDCRMPAFKVSAVRAEPLVVHEQMEGVLGT

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=706, Percent_Identity=32.1529745042493, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788534, Length=525, Percent_Identity=27.047619047619, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI3868719, Length=613, Percent_Identity=25.4486133768352, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI3868720, Length=586, Percent_Identity=26.2798634812287, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1787870, Length=506, Percent_Identity=25.296442687747, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87081797, Length=591, Percent_Identity=24.5346869712352, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI171474008, Length=554, Percent_Identity=23.826714801444, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1787778, Length=399, Percent_Identity=24.3107769423559, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87081994, Length=674, Percent_Identity=22.4035608308605, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 79284; Mature: 79284

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1 ; PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSGYLQEKRQPPNRKIVYNSQCPFCSVQCKMQLIERKHGQETSYDVVGIENPTSKGRLC
CCCCCCCHHCCCCCCEEEECCCCCEEHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEE
VKGLNAHQHAFHPDRLTQPLAKVNGKWLPISWDEALKQIAENVQAIQQEDGHDAMAVYGS
EECCCCCCCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
ASITNEEAYLLGKFARVALKTRHIDYNGRLCMSAAASAATTAFGIDRGLTNSLEDVLFAE
CCCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
CLMLVGTNLAECQPTLMPYIEQAKKNGAFLIVVDPRETATAQLADLHIKLKPGTDAALAN
HHHHHCCCHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEEECCCCCHHHHH
GLLKCILEGGYEDAAFIQKRVEGFAEVKAHIAKLKLENIAEQTGVSGDVITKAAETFAKA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RTGMIFTARGVEQQTNGSMAVRNFLNVLIATGKLGKHSCGFAAVTGQSNGQGAREHGQKA
HCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHCCHH
DQLPGYRSIENEEHRAHIAAIWGISPEQLPRKGVSAFEMMKKADKGEITGMFLMCSNPVV
HHCCCCCCCCCCCCCEEEEEEECCCHHHHHHCCHHHHHHHHHCCCCCEEEEEEEECCCCC
SHPQADFVKNALKKLKFLVAVDLFVSETAQLADLILPSTSYLEDEGTMTNVEGRVTLREA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCEECCCCEEEEEEC
SMPPYGEARHDWQIICNIAAVLGKGTDFSYSCAEDIFTELRMASKGGIADYYGITYERLR
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHH
KEKGIFWPCPSLEHPGTPLLFTDCFYRPNKKALMTVVPNDAPVPKEKPSRDYPLYLTTGR
HHCCCEECCCCCCCCCCCEEEEECEECCCCCEEEEECCCCCCCCCCCCCCCCCEEEEHHH
VLAHYLTGVQTRNSASLMARNFESFVEMHPETAKKHGIADRTLIKLTTKQGSMIVRCHFT
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCCEEEEEEEH
ETIREDTVFVPFHWSGKQNVNRIITGNLDPDCRMPAFKVSAVRAEPLVVHEQMEGVLGT
HHHCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCEEEHHHHHHCCCC
>Mature Secondary Structure
MQSGYLQEKRQPPNRKIVYNSQCPFCSVQCKMQLIERKHGQETSYDVVGIENPTSKGRLC
CCCCCCCHHCCCCCCEEEECCCCCEEHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEE
VKGLNAHQHAFHPDRLTQPLAKVNGKWLPISWDEALKQIAENVQAIQQEDGHDAMAVYGS
EECCCCCCCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
ASITNEEAYLLGKFARVALKTRHIDYNGRLCMSAAASAATTAFGIDRGLTNSLEDVLFAE
CCCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
CLMLVGTNLAECQPTLMPYIEQAKKNGAFLIVVDPRETATAQLADLHIKLKPGTDAALAN
HHHHHCCCHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEEECCCCCHHHHH
GLLKCILEGGYEDAAFIQKRVEGFAEVKAHIAKLKLENIAEQTGVSGDVITKAAETFAKA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RTGMIFTARGVEQQTNGSMAVRNFLNVLIATGKLGKHSCGFAAVTGQSNGQGAREHGQKA
HCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHCCHH
DQLPGYRSIENEEHRAHIAAIWGISPEQLPRKGVSAFEMMKKADKGEITGMFLMCSNPVV
HHCCCCCCCCCCCCCEEEEEEECCCHHHHHHCCHHHHHHHHHCCCCCEEEEEEEECCCCC
SHPQADFVKNALKKLKFLVAVDLFVSETAQLADLILPSTSYLEDEGTMTNVEGRVTLREA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCEECCCCEEEEEEC
SMPPYGEARHDWQIICNIAAVLGKGTDFSYSCAEDIFTELRMASKGGIADYYGITYERLR
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHH
KEKGIFWPCPSLEHPGTPLLFTDCFYRPNKKALMTVVPNDAPVPKEKPSRDYPLYLTTGR
HHCCCEECCCCCCCCCCCEEEEECEECCCCCEEEEECCCCCCCCCCCCCCCCCEEEEHHH
VLAHYLTGVQTRNSASLMARNFESFVEMHPETAKKHGIADRTLIKLTTKQGSMIVRCHFT
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCCEEEEEEEH
ETIREDTVFVPFHWSGKQNVNRIITGNLDPDCRMPAFKVSAVRAEPLVVHEQMEGVLGT
HHHCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCEEEHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 7868621 [H]