Click here to switch to the map view.

The map label for this gene is aspS

Identifier: 56963342

GI number: 56963342

Start: 1699830

End: 1701599

Strand: Direct

Name: aspS

Synonym: ABC1577

Alternate gene names: 56963342

Gene position: 1699830-1701599 (Clockwise)

Preceding gene: 56963341

Following gene: 56963343

Centisome position: 39.5

GC content: 47.8

Gene sequence:

>1770_bases
ATGAAACGTACACATCATTGTGGCCAACTTGACAAAACACTCGCAGGCCAACAGGTTCAACTAAAAGGGTGGGTGCAGCG
GCGCCGTGATTTAGGCCAGGTGATTTTTTTAGATCTTCGTGATCGCTCTGGTATTGTCCAAGTTGTGTGCAGTCCGGAAG
TCAGTGAAGAAGCGTTGAAAGCAGCTGACCGTGTCCGCAATGAGTATCTTGTCGCCGTTTCAGGCATTGTGGTGGAACGG
AATGAACAGGCCGTTAATGACAAGCTGGCAACTGGTGCTATCGAAGTTCACGTTCGTGAGCTCGAAATCCTGAATGTCTC
AAAATCTTTGCCATTCCAAATTGAAGCAGACACGGATGCAGCTGAAGACGTCCGTTTGAAATACCGCTATTTGGATTTGC
GTCGCCCTGATATGCAAGAAGCGTTCGCGATGCGTCACAAAATCATGAAAAGCGTTCGTGATTTTCTTGATGCAGATGGC
TATTTGGAGATTGAGACGCCGATGCTAACCAAAAGCACGCCAGAAGGAGCGCGCGATTATTTGGTGCCGAGCCGCGTCCA
TCATGGCCAGTTTTATGCGCTGCCACAATCGCCGCAAATTTTTAAGCAACTGTTGATGGTGTCCGGTTTTGAGAAGTACT
TCCAAATTGTCCGCTGTTTCCGTGATGAAGATTTGCGCGCAGACAGGCAGCCTGAATTTACGCAAATCGATATTGAAACA
AGCTTTCTTGATACAGAGGATATCTTGGCGATGACTGAAGCCATGATGGAAAAATTGCTGAAAGAAACACACGGGCTTGA
GCTAAAACGGCCTTTCTCGCGGATGACGTACGAGGAAGCGATGAACCGCTACGGTTCTGACAAGCCTGACACTCGTTTTG
GCATGGAACTGATCGAACTGTCTGACGTGCTCAAAGACACGGAGTTTAAAGTGTTTAAAGGCGCGTTAGAAGCAGGCGGC
ATTATTAAAGGCCTTACACTGAAAGGCGGGGCTGATAAGCTTTCACGAAAAGACATTGATGCGTTAGCTGATTTTGTCAA
GCCTTATGGGGCAAAAGGGTTGGCTTGGCTCAAAGTCGATGACGAAGGGATAAAGGGCCCGATTGCCAAATTCTTTAATG
AGGCGCAAACGGCTGCATTGCTTGCGGCAATGGACGCTGAAGCAGGCGATTTGCTCTTTTTCGGAGCCGATAAAAAGCAA
GTTGTGTTTCATGCATTGGGCGCGCTGCGCTTAAAGTTTGGAAAGGAGTTTCAGCTAATTGACGAAAACGCGTTTCACTT
CCTCTGGGTAACCGATTTTCCACTTGTCGAATATGACGAACAAGCAAAACGGTTTGTCGCGCTCCATCATCCGTTTACAC
GCCCGAAAGCGGAAGACTTGGACCTGATGGAAACCCATCCAGAGCAAGTACGTGCCGAAGCATATGACCTTGTCTTAAAC
GGCTTTGAGCTTGGCGGCGGCTCCCAGCGCATCTATGAACGGGAACTGCAAGAAAAAATGTTCAAGCTCCTTGGTTTTAG
TTCAGAAGCGGCTAAAGAAGAGTTTGGCTTTTTGCTTGAAGCATTTGATTATGGAACGCCTCCACATGGCGGCATTGCCT
TAGGTCTGGACCGAATCGTCATGCTTCTTGCACGCAAAAGCAATTTACGTGAGGTGATTGCTTTTCCAAAAACGGCAAGC
GCTAGCGACCTTCTAACCGAAGCGCCAAACAAAGTGAGCGTTGAACAGCTGATTGATTTAAACTTGTCCATTATTAGCAA
GCGCAGTTGA

Upstream 100 bases:

>100_bases
AGCGCTCGTTAAACAAATGGCAACTGGAGAACAAACGGTAGTCGCCATTAGCGATTTGACATCATTTTTACAAGAACAGA
TACGAGGAGGAAACTAGGAG

Downstream 100 bases:

>100_bases
CAAATACGTGGCCTCTGCTTATAATGTGCCTAATATAAAAATGAATAAGAACTTATCAAGAGTGGCGGAGGGACCTGACC
CAATGAAGCCCGGCAACCAG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MKRTHHCGQLDKTLAGQQVQLKGWVQRRRDLGQVIFLDLRDRSGIVQVVCSPEVSEEALKAADRVRNEYLVAVSGIVVER
NEQAVNDKLATGAIEVHVRELEILNVSKSLPFQIEADTDAAEDVRLKYRYLDLRRPDMQEAFAMRHKIMKSVRDFLDADG
YLEIETPMLTKSTPEGARDYLVPSRVHHGQFYALPQSPQIFKQLLMVSGFEKYFQIVRCFRDEDLRADRQPEFTQIDIET
SFLDTEDILAMTEAMMEKLLKETHGLELKRPFSRMTYEEAMNRYGSDKPDTRFGMELIELSDVLKDTEFKVFKGALEAGG
IIKGLTLKGGADKLSRKDIDALADFVKPYGAKGLAWLKVDDEGIKGPIAKFFNEAQTAALLAAMDAEAGDLLFFGADKKQ
VVFHALGALRLKFGKEFQLIDENAFHFLWVTDFPLVEYDEQAKRFVALHHPFTRPKAEDLDLMETHPEQVRAEAYDLVLN
GFELGGGSQRIYERELQEKMFKLLGFSSEAAKEEFGFLLEAFDYGTPPHGGIALGLDRIVMLLARKSNLREVIAFPKTAS
ASDLLTEAPNKVSVEQLIDLNLSIISKRS

Sequences:

>Translated_589_residues
MKRTHHCGQLDKTLAGQQVQLKGWVQRRRDLGQVIFLDLRDRSGIVQVVCSPEVSEEALKAADRVRNEYLVAVSGIVVER
NEQAVNDKLATGAIEVHVRELEILNVSKSLPFQIEADTDAAEDVRLKYRYLDLRRPDMQEAFAMRHKIMKSVRDFLDADG
YLEIETPMLTKSTPEGARDYLVPSRVHHGQFYALPQSPQIFKQLLMVSGFEKYFQIVRCFRDEDLRADRQPEFTQIDIET
SFLDTEDILAMTEAMMEKLLKETHGLELKRPFSRMTYEEAMNRYGSDKPDTRFGMELIELSDVLKDTEFKVFKGALEAGG
IIKGLTLKGGADKLSRKDIDALADFVKPYGAKGLAWLKVDDEGIKGPIAKFFNEAQTAALLAAMDAEAGDLLFFGADKKQ
VVFHALGALRLKFGKEFQLIDENAFHFLWVTDFPLVEYDEQAKRFVALHHPFTRPKAEDLDLMETHPEQVRAEAYDLVLN
GFELGGGSQRIYERELQEKMFKLLGFSSEAAKEEFGFLLEAFDYGTPPHGGIALGLDRIVMLLARKSNLREVIAFPKTAS
ASDLLTEAPNKVSVEQLIDLNLSIISKRS
>Mature_589_residues
MKRTHHCGQLDKTLAGQQVQLKGWVQRRRDLGQVIFLDLRDRSGIVQVVCSPEVSEEALKAADRVRNEYLVAVSGIVVER
NEQAVNDKLATGAIEVHVRELEILNVSKSLPFQIEADTDAAEDVRLKYRYLDLRRPDMQEAFAMRHKIMKSVRDFLDADG
YLEIETPMLTKSTPEGARDYLVPSRVHHGQFYALPQSPQIFKQLLMVSGFEKYFQIVRCFRDEDLRADRQPEFTQIDIET
SFLDTEDILAMTEAMMEKLLKETHGLELKRPFSRMTYEEAMNRYGSDKPDTRFGMELIELSDVLKDTEFKVFKGALEAGG
IIKGLTLKGGADKLSRKDIDALADFVKPYGAKGLAWLKVDDEGIKGPIAKFFNEAQTAALLAAMDAEAGDLLFFGADKKQ
VVFHALGALRLKFGKEFQLIDENAFHFLWVTDFPLVEYDEQAKRFVALHHPFTRPKAEDLDLMETHPEQVRAEAYDLVLN
GFELGGGSQRIYERELQEKMFKLLGFSSEAAKEEFGFLLEAFDYGTPPHGGIALGLDRIVMLLARKSNLREVIAFPKTAS
ASDLLTEAPNKVSVEQLIDLNLSIISKRS

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=588, Percent_Identity=41.6666666666667, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI45439306, Length=247, Percent_Identity=29.1497975708502, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI194272210, Length=246, Percent_Identity=27.2357723577236, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI5031815, Length=246, Percent_Identity=27.2357723577236, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI4758762, Length=287, Percent_Identity=27.1777003484321, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=52.4534686971235, Blast_Score=613, Evalue=1e-177,
Organism=Escherichia coli, GI1790571, Length=289, Percent_Identity=28.719723183391, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789256, Length=320, Percent_Identity=27.8125, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32566633, Length=599, Percent_Identity=34.3906510851419, Blast_Score=321, Evalue=6e-88,
Organism=Caenorhabditis elegans, GI71994340, Length=256, Percent_Identity=23.4375, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=256, Percent_Identity=27.734375, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=256, Percent_Identity=23.4375, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=256, Percent_Identity=23.4375, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71984122, Length=310, Percent_Identity=24.8387096774194, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=633, Percent_Identity=33.6492890995261, Blast_Score=293, Evalue=6e-80,
Organism=Saccharomyces cerevisiae, GI6320242, Length=267, Percent_Identity=26.9662921348315, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=287, Percent_Identity=27.1777003484321, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=245, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24584738, Length=605, Percent_Identity=38.5123966942149, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17136276, Length=255, Percent_Identity=29.8039215686275, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24640849, Length=263, Percent_Identity=28.5171102661597, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640851, Length=263, Percent_Identity=28.5171102661597, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19921528, Length=281, Percent_Identity=25.9786476868327, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_BACSK (Q5WHP3)

Other databases:

- EMBL:   AP006627
- RefSeq:   YP_175073.1
- ProteinModelPortal:   Q5WHP3
- SMR:   Q5WHP3
- STRING:   Q5WHP3
- EnsemblBacteria:   EBBACT00000047647
- GeneID:   3201736
- GenomeReviews:   AP006627_GR
- KEGG:   bcl:ABC1577
- NMPDR:   fig|66692.3.peg.1539
- eggNOG:   COG0173
- GeneTree:   EBGT00050000000858
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   BCLA66692:ABC1577-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66683; Mature: 66683

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTHHCGQLDKTLAGQQVQLKGWVQRRRDLGQVIFLDLRDRSGIVQVVCSPEVSEEALK
CCCCCCCCCHHHHHCCCEEHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCHHHHHHH
AADRVRNEYLVAVSGIVVERNEQAVNDKLATGAIEVHVRELEILNVSKSLPFQIEADTDA
HHHHHHHCEEEEEEEEEEECCCHHHHHHHHCCEEEEEEEEEEEEECCCCCCEEEECCCCC
AEDVRLKYRYLDLRRPDMQEAFAMRHKIMKSVRDFLDADGYLEIETPMLTKSTPEGARDY
CHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHC
LVPSRVHHGQFYALPQSPQIFKQLLMVSGFEKYFQIVRCFRDEDLRADRQPEFTQIDIET
CCCCCCCCCEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SFLDTEDILAMTEAMMEKLLKETHGLELKRPFSRMTYEEAMNRYGSDKPDTRFGMELIEL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
SDVLKDTEFKVFKGALEAGGIIKGLTLKGGADKLSRKDIDALADFVKPYGAKGLAWLKVD
HHHHCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
DEGIKGPIAKFFNEAQTAALLAAMDAEAGDLLFFGADKKQVVFHALGALRLKFGKEFQLI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEE
DENAFHFLWVTDFPLVEYDEQAKRFVALHHPFTRPKAEDLDLMETHPEQVRAEAYDLVLN
CCCCEEEEEEECCCCCCCCHHHHHEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHC
GFELGGGSQRIYERELQEKMFKLLGFSSEAAKEEFGFLLEAFDYGTPPHGGIALGLDRIV
CEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
MLLARKSNLREVIAFPKTASASDLLTEAPNKVSVEQLIDLNLSIISKRS
HHHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCCHHHHHCCCHHEEECCC
>Mature Secondary Structure
MKRTHHCGQLDKTLAGQQVQLKGWVQRRRDLGQVIFLDLRDRSGIVQVVCSPEVSEEALK
CCCCCCCCCHHHHHCCCEEHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCHHHHHHH
AADRVRNEYLVAVSGIVVERNEQAVNDKLATGAIEVHVRELEILNVSKSLPFQIEADTDA
HHHHHHHCEEEEEEEEEEECCCHHHHHHHHCCEEEEEEEEEEEEECCCCCCEEEECCCCC
AEDVRLKYRYLDLRRPDMQEAFAMRHKIMKSVRDFLDADGYLEIETPMLTKSTPEGARDY
CHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHC
LVPSRVHHGQFYALPQSPQIFKQLLMVSGFEKYFQIVRCFRDEDLRADRQPEFTQIDIET
CCCCCCCCCEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SFLDTEDILAMTEAMMEKLLKETHGLELKRPFSRMTYEEAMNRYGSDKPDTRFGMELIEL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
SDVLKDTEFKVFKGALEAGGIIKGLTLKGGADKLSRKDIDALADFVKPYGAKGLAWLKVD
HHHHCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
DEGIKGPIAKFFNEAQTAALLAAMDAEAGDLLFFGADKKQVVFHALGALRLKFGKEFQLI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEE
DENAFHFLWVTDFPLVEYDEQAKRFVALHHPFTRPKAEDLDLMETHPEQVRAEAYDLVLN
CCCCEEEEEEECCCCCCCCHHHHHEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHC
GFELGGGSQRIYERELQEKMFKLLGFSSEAAKEEFGFLLEAFDYGTPPHGGIALGLDRIV
CEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
MLLARKSNLREVIAFPKTASASDLLTEAPNKVSVEQLIDLNLSIISKRS
HHHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCCHHHHHCCCHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA