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The map label for this gene is queA

Identifier: 56963322

GI number: 56963322

Start: 1679405

End: 1680433

Strand: Direct

Name: queA

Synonym: ABC1557

Alternate gene names: 56963322

Gene position: 1679405-1680433 (Clockwise)

Preceding gene: 56963321

Following gene: 56963323

Centisome position: 39.02

GC content: 46.65

Gene sequence:

>1029_bases
ATGGACGTAAATGAATTTGATTTTGATTTACCTGAAGAATTAATTGCCCAAACTCCTTTGAAGACTAGGACGAATTCCCG
TTTGCTTGTGGTTGACAAAAAAACAAAAGCGATAAGCGACCGGATGTTTCCTGATTTGATTGAAGCGCTTTCGCCAGGAG
ATTGCCTTGTCTTAAATGATACGAAAGTGATGCCTGCCCGCCTTTTAGGCGAAAAGGTAGAAACGAAAGCAGTCATCGAG
CTGTTGCTTCTAAAACAGCTTGAAGGCGACTGTTGGGAAACGCTTGTAAAGCCAGCAAAGCGAGTAAAAATAGGGACAGA
GGTTAGCTTTGGCGACGGAAAGTTAACAGCGATATGCACGGCAGTCCAACCCCAAGGGGGGAGGATTTTCGAATTCCGCT
ACAGTGGTGTGTTTTATGAATTGTTGGAGGAACTAGGGACGATGCCATTGCCTCCTTATATTAAAGAACGGCTAGAAGAT
GCCGACCGTTACCAGACTGTGTATGCGAAGCAGATAGGTTCGGCCGCTGCGCCAACGGCTGGCCTTCACTTTAGCGAGCA
ATTGCTTTTAGAGATCGAAGCAAAAGGCGTAAAAATTGTGTATATTACCCTTCATGTCGGTTTGGGGACATTTCGCCCAG
TGAGCGCTAGTTCTGTTGAAGACCACCAAATGCATGCCGAGTATTACCAGGTGACAGAAGAAGCGGCCCACACGCTGGCT
TCTGCCCGGGAGAATGGCGGCAACATTATCGCAGTTGGGACAACCTCAGCAAGAACGCTTGAAACGATCTATAGCGATCA
TGGCGCCTTTGTAGCGGCATCTGGCTGGACGTCGATCTTTATTTATCCTGGCTATAAGTACCAGGCAATTGACGGGTTGT
TGACGAATTTCCATTTGCCAAAATCAACGCTTGTCATGCTTGTTAGCGCATTGATAGGCAAAGAGGCATTAATGGATGCT
TATGCACACGCCATAAAAGAACGCTACCGATTTTTTAGCTTCGGCGACGCAATGTTTATACGACCGTAA

Upstream 100 bases:

>100_bases
TTTCTCACTCTTCCGCCGTTAGAAGTGCTTGCGCTAAATGAAAAACGCATGTATGCTAGGAAAAGTTTGATTGGAATGCG
TAAGATAGATAGGTGATGAC

Downstream 100 bases:

>100_bases
GGGGAGACAAATAGTGACAGCAGCAATCACCTATGAACACATAAAAACATGTAAGCAATCTGGCGCTCGGCTCGGTCGTG
TCCATACGCCTCATGGCACG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MDVNEFDFDLPEELIAQTPLKTRTNSRLLVVDKKTKAISDRMFPDLIEALSPGDCLVLNDTKVMPARLLGEKVETKAVIE
LLLLKQLEGDCWETLVKPAKRVKIGTEVSFGDGKLTAICTAVQPQGGRIFEFRYSGVFYELLEELGTMPLPPYIKERLED
ADRYQTVYAKQIGSAAAPTAGLHFSEQLLLEIEAKGVKIVYITLHVGLGTFRPVSASSVEDHQMHAEYYQVTEEAAHTLA
SARENGGNIIAVGTTSARTLETIYSDHGAFVAASGWTSIFIYPGYKYQAIDGLLTNFHLPKSTLVMLVSALIGKEALMDA
YAHAIKERYRFFSFGDAMFIRP

Sequences:

>Translated_342_residues
MDVNEFDFDLPEELIAQTPLKTRTNSRLLVVDKKTKAISDRMFPDLIEALSPGDCLVLNDTKVMPARLLGEKVETKAVIE
LLLLKQLEGDCWETLVKPAKRVKIGTEVSFGDGKLTAICTAVQPQGGRIFEFRYSGVFYELLEELGTMPLPPYIKERLED
ADRYQTVYAKQIGSAAAPTAGLHFSEQLLLEIEAKGVKIVYITLHVGLGTFRPVSASSVEDHQMHAEYYQVTEEAAHTLA
SARENGGNIIAVGTTSARTLETIYSDHGAFVAASGWTSIFIYPGYKYQAIDGLLTNFHLPKSTLVMLVSALIGKEALMDA
YAHAIKERYRFFSFGDAMFIRP
>Mature_342_residues
MDVNEFDFDLPEELIAQTPLKTRTNSRLLVVDKKTKAISDRMFPDLIEALSPGDCLVLNDTKVMPARLLGEKVETKAVIE
LLLLKQLEGDCWETLVKPAKRVKIGTEVSFGDGKLTAICTAVQPQGGRIFEFRYSGVFYELLEELGTMPLPPYIKERLED
ADRYQTVYAKQIGSAAAPTAGLHFSEQLLLEIEAKGVKIVYITLHVGLGTFRPVSASSVEDHQMHAEYYQVTEEAAHTLA
SARENGGNIIAVGTTSARTLETIYSDHGAFVAASGWTSIFIYPGYKYQAIDGLLTNFHLPKSTLVMLVSALIGKEALMDA
YAHAIKERYRFFSFGDAMFIRP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=351, Percent_Identity=45.2991452991453, Blast_Score=303, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_BACSK (Q5WHR3)

Other databases:

- EMBL:   AP006627
- RefSeq:   YP_175053.1
- ProteinModelPortal:   Q5WHR3
- SMR:   Q5WHR3
- STRING:   Q5WHR3
- EnsemblBacteria:   EBBACT00000047996
- GeneID:   3202840
- GenomeReviews:   AP006627_GR
- KEGG:   bcl:ABC1557
- NMPDR:   fig|66692.3.peg.1519
- eggNOG:   COG0809
- GeneTree:   EBGT00050000002867
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- BioCyc:   BCLA66692:ABC1557-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37923; Mature: 37923

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVNEFDFDLPEELIAQTPLKTRTNSRLLVVDKKTKAISDRMFPDLIEALSPGDCLVLND
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEC
TKVMPARLLGEKVETKAVIELLLLKQLEGDCWETLVKPAKRVKIGTEVSFGDGKLTAICT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEECCEEECCCCCEEEEEE
AVQPQGGRIFEFRYSGVFYELLEELGTMPLPPYIKERLEDADRYQTVYAKQIGSAAAPTA
EECCCCCEEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GLHFSEQLLLEIEAKGVKIVYITLHVGLGTFRPVSASSVEDHQMHAEYYQVTEEAAHTLA
CCCCCCEEEEEEECCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SARENGGNIIAVGTTSARTLETIYSDHGAFVAASGWTSIFIYPGYKYQAIDGLLTNFHLP
HHHCCCCCEEEEECCCHHHHHHHHCCCCCEEEECCCEEEEEECCCEEHHHHHHHHHCCCC
KSTLVMLVSALIGKEALMDAYAHAIKERYRFFSFGDAMFIRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEECC
>Mature Secondary Structure
MDVNEFDFDLPEELIAQTPLKTRTNSRLLVVDKKTKAISDRMFPDLIEALSPGDCLVLND
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEC
TKVMPARLLGEKVETKAVIELLLLKQLEGDCWETLVKPAKRVKIGTEVSFGDGKLTAICT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEECCEEECCCCCEEEEEE
AVQPQGGRIFEFRYSGVFYELLEELGTMPLPPYIKERLEDADRYQTVYAKQIGSAAAPTA
EECCCCCEEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GLHFSEQLLLEIEAKGVKIVYITLHVGLGTFRPVSASSVEDHQMHAEYYQVTEEAAHTLA
CCCCCCEEEEEEECCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SARENGGNIIAVGTTSARTLETIYSDHGAFVAASGWTSIFIYPGYKYQAIDGLLTNFHLP
HHHCCCCCEEEEECCCHHHHHHHHCCCCCEEEECCCEEEEEECCCEEHHHHHHHHHCCCC
KSTLVMLVSALIGKEALMDAYAHAIKERYRFFSFGDAMFIRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA