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The map label for this gene is tgt

Identifier: 56963323

GI number: 56963323

Start: 1680447

End: 1681598

Strand: Direct

Name: tgt

Synonym: ABC1558

Alternate gene names: 56963323

Gene position: 1680447-1681598 (Clockwise)

Preceding gene: 56963322

Following gene: 56963324

Centisome position: 39.05

GC content: 46.53

Gene sequence:

>1152_bases
GTGACAGCAGCAATCACCTATGAACACATAAAAACATGTAAGCAATCTGGCGCTCGGCTCGGTCGTGTCCATACGCCTCA
TGGCACGATTGAAACGCCGATGTTTATGCCGGTAGGTACGTTGGCGACTGTGAAAACGATGAGTCCAGAAGACTTGAAAG
CAATGGATGCACAAATTATTTTAAGCAACACCTACCACTTATGGCTTCGCCCGGGAGAAGAGATTATTAAGGAAGCTGGC
GGCCTTCATCAGTTTATGAATTGGGATAAGCCGATCTTAACGGATTCTGGTGGCTTCCAAGTCTTTTCCCTTAGTGATTT
ACGCAAGATCACGGAAGAAGGCGTCCATTTCCGCAATCATTTGAGTGGTGAAAAGCTGTTTCTTTCGCCAGAGAAAGCGA
TGCAAATCCAACATGTGCTTGGATCGGACATTATGATGGCATTCGATGAATGTCCACCTTACCCTGCCGACCATAGCTAT
ATGAAAGCTTCGGTCGAACGGACAAGCCGCTGGGCAGAACGGTGCCTTGCCGAACACAAACGTTCAGGCAAAGACAGTGT
TCAAGGGTTATTTGGTATCATTCAAGGCGGTGAATACGAAGATCTGCGCAAACAGAGTGCAAATGATTTGACAGCGCTTG
ATTTTCCAGGTTACGCTGTAGGTGGACTGTCTGTCGGAGAACCGAAAGATGTCATGAATCATGTGCTTGAGTTCACAACG
CCATTAATGCCGGAAGATAAACCTAGGTATTTAATGGGAGTCGGCTCGCCAGATTCACTCATTGACGGGGCGATAAGGGG
CATTGATATGTTTGACTGCGTCTTGCCGACCCGAATAGCGAGAAACGGTACTTGCATGACAAGTGCGGGAAGATTGGTTG
TCCGTAATGCTAAGTACGCGCGTGATTTCAGTCCCCTCGATGAAAAGTGTGACTGCCATGTGTGCCGTACGTACTCGCGG
GCGTATATTCGTCATCTCATTAAATGCGAAGAAACATTTGGGTTCCGTTTAACGACGTACCACAACCTTTATTTTCTTTT
AAATTTAATGAAGCAAGTTCGCCAAGCGATTATGGATGACTGCCTTCTTGATTTTCGCGAGCAGTTTTTTGAGGAATACG
GCTTTAACAAGGAAAACGCACGCAACTTCTAG

Upstream 100 bases:

>100_bases
GAGGCATTAATGGATGCTTATGCACACGCCATAAAAGAACGCTACCGATTTTTTAGCTTCGGCGACGCAATGTTTATACG
ACCGTAAGGGGAGACAAATA

Downstream 100 bases:

>100_bases
TTCTCTAGAAAAGGTGGCGTTAAAGCAAGGAAAGGGGGGTGTTTCATGGAATTACTTGTCAGTATGCTTCCGTTTATCGC
GATTATCGTGCTATTTTATT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTAAITYEHIKTCKQSGARLGRVHTPHGTIETPMFMPVGTLATVKTMSPEDLKAMDAQIILSNTYHLWLRPGEEIIKEAG
GLHQFMNWDKPILTDSGGFQVFSLSDLRKITEEGVHFRNHLSGEKLFLSPEKAMQIQHVLGSDIMMAFDECPPYPADHSY
MKASVERTSRWAERCLAEHKRSGKDSVQGLFGIIQGGEYEDLRKQSANDLTALDFPGYAVGGLSVGEPKDVMNHVLEFTT
PLMPEDKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSAGRLVVRNAKYARDFSPLDEKCDCHVCRTYSR
AYIRHLIKCEETFGFRLTTYHNLYFLLNLMKQVRQAIMDDCLLDFREQFFEEYGFNKENARNF

Sequences:

>Translated_383_residues
MTAAITYEHIKTCKQSGARLGRVHTPHGTIETPMFMPVGTLATVKTMSPEDLKAMDAQIILSNTYHLWLRPGEEIIKEAG
GLHQFMNWDKPILTDSGGFQVFSLSDLRKITEEGVHFRNHLSGEKLFLSPEKAMQIQHVLGSDIMMAFDECPPYPADHSY
MKASVERTSRWAERCLAEHKRSGKDSVQGLFGIIQGGEYEDLRKQSANDLTALDFPGYAVGGLSVGEPKDVMNHVLEFTT
PLMPEDKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSAGRLVVRNAKYARDFSPLDEKCDCHVCRTYSR
AYIRHLIKCEETFGFRLTTYHNLYFLLNLMKQVRQAIMDDCLLDFREQFFEEYGFNKENARNF
>Mature_382_residues
TAAITYEHIKTCKQSGARLGRVHTPHGTIETPMFMPVGTLATVKTMSPEDLKAMDAQIILSNTYHLWLRPGEEIIKEAGG
LHQFMNWDKPILTDSGGFQVFSLSDLRKITEEGVHFRNHLSGEKLFLSPEKAMQIQHVLGSDIMMAFDECPPYPADHSYM
KASVERTSRWAERCLAEHKRSGKDSVQGLFGIIQGGEYEDLRKQSANDLTALDFPGYAVGGLSVGEPKDVMNHVLEFTTP
LMPEDKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSAGRLVVRNAKYARDFSPLDEKCDCHVCRTYSRA
YIRHLIKCEETFGFRLTTYHNLYFLLNLMKQVRQAIMDDCLLDFREQFFEEYGFNKENARNF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=361, Percent_Identity=45.1523545706371, Blast_Score=331, Evalue=8e-91,
Organism=Homo sapiens, GI13375872, Length=286, Percent_Identity=24.8251748251748, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1786607, Length=357, Percent_Identity=57.1428571428571, Blast_Score=420, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17544680, Length=357, Percent_Identity=46.4985994397759, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI25146502, Length=219, Percent_Identity=26.4840182648402, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920510, Length=369, Percent_Identity=42.8184281842818, Blast_Score=311, Evalue=4e-85,
Organism=Drosophila melanogaster, GI21355311, Length=258, Percent_Identity=24.8062015503876, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_BACSK (Q5WHR2)

Other databases:

- EMBL:   AP006627
- RefSeq:   YP_175054.1
- ProteinModelPortal:   Q5WHR2
- SMR:   Q5WHR2
- STRING:   Q5WHR2
- EnsemblBacteria:   EBBACT00000046002
- GeneID:   3203410
- GenomeReviews:   AP006627_GR
- KEGG:   bcl:ABC1558
- NMPDR:   fig|66692.3.peg.1520
- eggNOG:   COG0343
- GeneTree:   EBGT00050000001431
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   BCLA66692:ABC1558-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 43432; Mature: 43301

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: ACT_SITE 95-95 BINDING 96-96

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAITYEHIKTCKQSGARLGRVHTPHGTIETPMFMPVGTLATVKTMSPEDLKAMDAQII
CCCEEEHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCHHHEECCCCHHHHHHCCEEE
LSNTYHLWLRPGEEIIKEAGGLHQFMNWDKPILTDSGGFQVFSLSDLRKITEEGVHFRNH
EECCEEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHC
LSGEKLFLSPEKAMQIQHVLGSDIMMAFDECPPYPADHSYMKASVERTSRWAERCLAEHK
CCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RSGKDSVQGLFGIIQGGEYEDLRKQSANDLTALDFPGYAVGGLSVGEPKDVMNHVLEFTT
CCCHHHHHHHHHHHCCCCHHHHHHCCCCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHC
PLMPEDKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSAGRLVVRNAKYA
CCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEECCHHH
RDFSPLDEKCDCHVCRTYSRAYIRHLIKCEETFGFRLTTYHNLYFLLNLMKQVRQAIMDD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHH
CLLDFREQFFEEYGFNKENARNF
HHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TAAITYEHIKTCKQSGARLGRVHTPHGTIETPMFMPVGTLATVKTMSPEDLKAMDAQII
CCEEEHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCHHHEECCCCHHHHHHCCEEE
LSNTYHLWLRPGEEIIKEAGGLHQFMNWDKPILTDSGGFQVFSLSDLRKITEEGVHFRNH
EECCEEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHC
LSGEKLFLSPEKAMQIQHVLGSDIMMAFDECPPYPADHSYMKASVERTSRWAERCLAEHK
CCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RSGKDSVQGLFGIIQGGEYEDLRKQSANDLTALDFPGYAVGGLSVGEPKDVMNHVLEFTT
CCCHHHHHHHHHHHCCCCHHHHHHCCCCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHC
PLMPEDKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSAGRLVVRNAKYA
CCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEECCHHH
RDFSPLDEKCDCHVCRTYSRAYIRHLIKCEETFGFRLTTYHNLYFLLNLMKQVRQAIMDD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHH
CLLDFREQFFEEYGFNKENARNF
HHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA