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The map label for this gene is nadB [H]

Identifier: 56963312

GI number: 56963312

Start: 1670038

End: 1671570

Strand: Direct

Name: nadB [H]

Synonym: ABC1547

Alternate gene names: 56963312

Gene position: 1670038-1671570 (Clockwise)

Preceding gene: 56963309

Following gene: 56963313

Centisome position: 38.8

GC content: 48.21

Gene sequence:

>1533_bases
ATGAAAAAGAAATCAGTTATTATTGTTGGCGCCGGTATAGCTGGACTAATGGCTGCCCACAAATTAGCAGATAAAGCAGA
AGTATCGCTTTTAATGAAGGCGGGCAGGTTGGACAGCAATTCTTGTATGGCTCAAGGGGGGATTGCGGCGGCAGTAGGCG
AAACCGACCATCCGACACTCCATGCGAGAGATACGTTTGTCGCAGGCAGCGGTTTTAATGCGTTTAAGCGTGCGTCTGAG
CTCGCTCGGCAGGCTCCAGCAATGATTAAAGAGCTCGATGCTTTAGGGGTGCCGTTTGAAAAAGCAGCTGGCCAATATGT
CCTTGGCCATGAAGGAGCCCATAGCAAGCGGCGCATTTTGCATGCTTATCAAGATCAAACAGGCTTAGCAGTTGTCGAAA
CATTATGGCGGACACTCAGTGAAAACGTGCATGTGTACGAAAACCAATATGTGCTGGAGTTGATTGTGAAAAATCAAACA
ATCCAAGGCGTTCGCACCGTTAATCACGCTTTTTACGGCGACTGTGTGGTTATAGCAACTGGCGGCTCTGGCCAATTGTA
TGAAAATAGTTCCAATGAGCAGAGAGCAACGGGAGACGGGTTAATGTTAGCTTATCGGGCTGGCGCTGTCCTTAAAGATC
TTGAGTTCATGCAATTCCATCCGACGATTTTAAAAGGAAAAGAACCGAGCCTTATTAGCGAAGCAGTCCGCGGTGAAGGT
GCTTACTTGGTCAATCGGGCAGGCCATCGGCTAATGGGAAAATGGGCAAAAGGGGAACTTGAAGGGCGGGACGTTGTAGC
GGCCACATTACATAAAGCGTTGCAATCTGGCGAGAATGTTTATTTAGATGCTAGGCATTTGCATGATTTTCCAAGTCGGT
TTCCTGCGATTTATCGACGTCTGCAGCGAAATGGCTATGATGTTTGCAAAGACTTAATCCCGATTGAACCAGGCGCCCAT
TTTTTATGCGGTGGCATTGAGACAGACCGATTTGGGCGCACGTCGGTAAATGGGTTGTACGCGATAGGCGAAGCAGCTTG
TACGGGTGTTCATGGCGCAAATCGTTTAGCTAGCAATTCACTCGTCGAAGCACTTGTTTACGGGCAAGCAGTGGCAGCGG
CTTTGTTGGCCGATCCACAAACAAAAGCGGGAGCGGTAAAGCCACAGCCGAATAAGGCACTGATACACGGGCTTCCTGAA
CGTAGCGAATTGCAAAAAATGATGAGCGCCTATGCAGGCATTGAACGCGACGCCTATCGATTGCAAACAGTGGCTAATTG
GCTTTCTTCGTTTCAACAAATGCCCAAGAAAATGGTTGTGCACGTTGACGATCTAGAAACGGGTACTATGTATGAGCTTG
CTACGCTTGTTGTAAAAGCAGCTTTGTTGCGTACGGAAACAAGAGGCACCCATAGGCGCAGCGATTATCCGTCAGCAGAC
GAACAATGGCGGAAGCGCTCGATTTGTTTTTCTGTACAGAATAAAGCATTCGTGAAAACAGAGGAGAGAGAATATGAACC
CGCTCAAGCTTGA

Upstream 100 bases:

>100_bases
CTAAATAATACATGGCAATCTCCATTCTTAAAAAAAGGTTGTGTTTTCTATTAGTTTATGTAAATATAGGTGACAAGACA
ACTGTAAAGTGAGTGAGTCT

Downstream 100 bases:

>100_bases
TCAGCAGTTAAGGGGATTTTTGGCAGAAGACATTGGTTATGGAGACCGTACAGCTGAAGCGCTGTTCACTGTTGAAAGAG
CAGAGGCCGTTATAGTTGCC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MKKKSVIIVGAGIAGLMAAHKLADKAEVSLLMKAGRLDSNSCMAQGGIAAAVGETDHPTLHARDTFVAGSGFNAFKRASE
LARQAPAMIKELDALGVPFEKAAGQYVLGHEGAHSKRRILHAYQDQTGLAVVETLWRTLSENVHVYENQYVLELIVKNQT
IQGVRTVNHAFYGDCVVIATGGSGQLYENSSNEQRATGDGLMLAYRAGAVLKDLEFMQFHPTILKGKEPSLISEAVRGEG
AYLVNRAGHRLMGKWAKGELEGRDVVAATLHKALQSGENVYLDARHLHDFPSRFPAIYRRLQRNGYDVCKDLIPIEPGAH
FLCGGIETDRFGRTSVNGLYAIGEAACTGVHGANRLASNSLVEALVYGQAVAAALLADPQTKAGAVKPQPNKALIHGLPE
RSELQKMMSAYAGIERDAYRLQTVANWLSSFQQMPKKMVVHVDDLETGTMYELATLVVKAALLRTETRGTHRRSDYPSAD
EQWRKRSICFSVQNKAFVKTEEREYEPAQA

Sequences:

>Translated_510_residues
MKKKSVIIVGAGIAGLMAAHKLADKAEVSLLMKAGRLDSNSCMAQGGIAAAVGETDHPTLHARDTFVAGSGFNAFKRASE
LARQAPAMIKELDALGVPFEKAAGQYVLGHEGAHSKRRILHAYQDQTGLAVVETLWRTLSENVHVYENQYVLELIVKNQT
IQGVRTVNHAFYGDCVVIATGGSGQLYENSSNEQRATGDGLMLAYRAGAVLKDLEFMQFHPTILKGKEPSLISEAVRGEG
AYLVNRAGHRLMGKWAKGELEGRDVVAATLHKALQSGENVYLDARHLHDFPSRFPAIYRRLQRNGYDVCKDLIPIEPGAH
FLCGGIETDRFGRTSVNGLYAIGEAACTGVHGANRLASNSLVEALVYGQAVAAALLADPQTKAGAVKPQPNKALIHGLPE
RSELQKMMSAYAGIERDAYRLQTVANWLSSFQQMPKKMVVHVDDLETGTMYELATLVVKAALLRTETRGTHRRSDYPSAD
EQWRKRSICFSVQNKAFVKTEEREYEPAQA
>Mature_510_residues
MKKKSVIIVGAGIAGLMAAHKLADKAEVSLLMKAGRLDSNSCMAQGGIAAAVGETDHPTLHARDTFVAGSGFNAFKRASE
LARQAPAMIKELDALGVPFEKAAGQYVLGHEGAHSKRRILHAYQDQTGLAVVETLWRTLSENVHVYENQYVLELIVKNQT
IQGVRTVNHAFYGDCVVIATGGSGQLYENSSNEQRATGDGLMLAYRAGAVLKDLEFMQFHPTILKGKEPSLISEAVRGEG
AYLVNRAGHRLMGKWAKGELEGRDVVAATLHKALQSGENVYLDARHLHDFPSRFPAIYRRLQRNGYDVCKDLIPIEPGAH
FLCGGIETDRFGRTSVNGLYAIGEAACTGVHGANRLASNSLVEALVYGQAVAAALLADPQTKAGAVKPQPNKALIHGLPE
RSELQKMMSAYAGIERDAYRLQTVANWLSSFQQMPKKMVVHVDDLETGTMYELATLVVKAALLRTETRGTHRRSDYPSAD
EQWRKRSICFSVQNKAFVKTEEREYEPAQA

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=536, Percent_Identity=31.1567164179104, Blast_Score=199, Evalue=5e-51,
Organism=Escherichia coli, GI1788928, Length=512, Percent_Identity=36.9140625, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1790597, Length=513, Percent_Identity=29.4346978557505, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786942, Length=530, Percent_Identity=30.7547169811321, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17550100, Length=540, Percent_Identity=31.6666666666667, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17505833, Length=538, Percent_Identity=29.9256505576208, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71986328, Length=450, Percent_Identity=24.6666666666667, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322701, Length=521, Percent_Identity=32.2456813819578, Blast_Score=202, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6322416, Length=542, Percent_Identity=31.549815498155, Blast_Score=194, Evalue=3e-50,
Organism=Drosophila melanogaster, GI17137288, Length=567, Percent_Identity=29.8059964726631, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24655642, Length=567, Percent_Identity=29.8059964726631, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24655647, Length=567, Percent_Identity=29.8059964726631, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24663005, Length=591, Percent_Identity=29.6108291032149, Blast_Score=187, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 55728; Mature: 55728

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKSVIIVGAGIAGLMAAHKLADKAEVSLLMKAGRLDSNSCMAQGGIAAAVGETDHPTL
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEEEECCCCCCCEE
HARDTFVAGSGFNAFKRASELARQAPAMIKELDALGVPFEKAAGQYVLGHEGAHSKRRIL
EECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEECCCCCCHHHHHH
HAYQDQTGLAVVETLWRTLSENVHVYENQYVLELIVKNQTIQGVRTVNHAFYGDCVVIAT
HHHCCCCCHHHHHHHHHHHCCCEEEECCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEE
GGSGQLYENSSNEQRATGDGLMLAYRAGAVLKDLEFMQFHPTILKGKEPSLISEAVRGEG
CCCCCEEECCCCCCCCCCCCEEEEEHHCHHHHHHHHHHHCCHHCCCCCCHHHHHHHCCCC
AYLVNRAGHRLMGKWAKGELEGRDVVAATLHKALQSGENVYLDARHLHDFPSRFPAIYRR
CEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHH
LQRNGYDVCKDLIPIEPGAHFLCGGIETDRFGRTSVNGLYAIGEAACTGVHGANRLASNS
HHHCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEHHHHHCCCCCHHHHHHHH
LVEALVYGQAVAAALLADPQTKAGAVKPQPNKALIHGLPERSELQKMMSAYAGIERDAYR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
LQTVANWLSSFQQMPKKMVVHVDDLETGTMYELATLVVKAALLRTETRGTHRRSDYPSAD
HHHHHHHHHHHHHCHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
EQWRKRSICFSVQNKAFVKTEEREYEPAQA
HHHHHHHHEEEECCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MKKKSVIIVGAGIAGLMAAHKLADKAEVSLLMKAGRLDSNSCMAQGGIAAAVGETDHPTL
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEEEECCCCCCCEE
HARDTFVAGSGFNAFKRASELARQAPAMIKELDALGVPFEKAAGQYVLGHEGAHSKRRIL
EECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEECCCCCCHHHHHH
HAYQDQTGLAVVETLWRTLSENVHVYENQYVLELIVKNQTIQGVRTVNHAFYGDCVVIAT
HHHCCCCCHHHHHHHHHHHCCCEEEECCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEE
GGSGQLYENSSNEQRATGDGLMLAYRAGAVLKDLEFMQFHPTILKGKEPSLISEAVRGEG
CCCCCEEECCCCCCCCCCCCEEEEEHHCHHHHHHHHHHHCCHHCCCCCCHHHHHHHCCCC
AYLVNRAGHRLMGKWAKGELEGRDVVAATLHKALQSGENVYLDARHLHDFPSRFPAIYRR
CEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHH
LQRNGYDVCKDLIPIEPGAHFLCGGIETDRFGRTSVNGLYAIGEAACTGVHGANRLASNS
HHHCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEHHHHHCCCCCHHHHHHHH
LVEALVYGQAVAAALLADPQTKAGAVKPQPNKALIHGLPERSELQKMMSAYAGIERDAYR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
LQTVANWLSSFQQMPKKMVVHVDDLETGTMYELATLVVKAALLRTETRGTHRRSDYPSAD
HHHHHHHHHHHHHCHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
EQWRKRSICFSVQNKAFVKTEEREYEPAQA
HHHHHHHHEEEECCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]