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The map label for this gene is ogt [H]

Identifier: 56963232

GI number: 56963232

Start: 1584997

End: 1585524

Strand: Direct

Name: ogt [H]

Synonym: ABC1467

Alternate gene names: 56963232

Gene position: 1584997-1585524 (Clockwise)

Preceding gene: 56963231

Following gene: 56963234

Centisome position: 36.83

GC content: 44.32

Gene sequence:

>528_bases
ATGAGTTCGCTTAAGCAAATGTACGTCAAAGAAATGGAGAGTCCCCTTGGATTGTTGTCCATTGTCGCATCGGAGCGAGG
AGTTTGCCAAATCCACTTTGGTGAAATAAACAAAGCACAAGTTACGGCAAAGCTGTCAAAAGCGGGAATCCGGCCTCAAT
GGATTCATTTAGACGAAAATGAAGATCTTGATATTTGTGGGCAGTTGCGCGAATACTTTAATGGTCTCCGTGTTGAGTTT
GATGCCGACATCGATTTAATCGGTACGCCGTTTCAACTAAAAGTGTGGAAAGAACTACAAAATATTGGCTATGGTAAAAC
AAAATCGTACAAAGATATTGCCCAGGCAATAGGAGCGCCAAAAGCCGTTCGCGCAGTGGGAGGCGCCAATAATCAAAACC
CCCTTCCTATATTGATCCCATGCCATAGAGTAATCGGCAGCAATGGGGCAATGGTTGGGTACGGAGGCGGCTTGACGAAA
AAGCAACTATTACTAGGCCTCGAAGGAGCATTAAAAATATCCTCATAA

Upstream 100 bases:

>100_bases
GTGCAAATTGAAATCGAAAAAGGGCTCGATCTGTTGAAAAAACAGAATGGGTCCCAATAATAAAGACGGCTTTAATCTTT
TCAGGGAAGAAGGGAATGGC

Downstream 100 bases:

>100_bases
AACAAGGCAGTGTGCTTATGCACACTGCCTTGTTTGCCTTTCCTTTAGCATCTTTTTTAACAAATACAAGTCAGATTGAG
CGTTGTACCCAGAAATGCTG

Product: methylated-DNA-[protein]-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MSSLKQMYVKEMESPLGLLSIVASERGVCQIHFGEINKAQVTAKLSKAGIRPQWIHLDENEDLDICGQLREYFNGLRVEF
DADIDLIGTPFQLKVWKELQNIGYGKTKSYKDIAQAIGAPKAVRAVGGANNQNPLPILIPCHRVIGSNGAMVGYGGGLTK
KQLLLGLEGALKISS

Sequences:

>Translated_175_residues
MSSLKQMYVKEMESPLGLLSIVASERGVCQIHFGEINKAQVTAKLSKAGIRPQWIHLDENEDLDICGQLREYFNGLRVEF
DADIDLIGTPFQLKVWKELQNIGYGKTKSYKDIAQAIGAPKAVRAVGGANNQNPLPILIPCHRVIGSNGAMVGYGGGLTK
KQLLLGLEGALKISS
>Mature_174_residues
SSLKQMYVKEMESPLGLLSIVASERGVCQIHFGEINKAQVTAKLSKAGIRPQWIHLDENEDLDICGQLREYFNGLRVEFD
ADIDLIGTPFQLKVWKELQNIGYGKTKSYKDIAQAIGAPKAVRAVGGANNQNPLPILIPCHRVIGSNGAMVGYGGGLTKK
QLLLGLEGALKISS

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=174, Percent_Identity=35.0574712643678, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=49.5238095238095, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1788542, Length=159, Percent_Identity=37.7358490566038, Blast_Score=96, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=82, Percent_Identity=51.219512195122, Blast_Score=87, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=51.219512195122, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=48.1927710843374, Blast_Score=84, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17137554, Length=87, Percent_Identity=48.2758620689655, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19035; Mature: 18904

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLKQMYVKEMESPLGLLSIVASERGVCQIHFGEINKAQVTAKLSKAGIRPQWIHLDEN
CCHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCC
EDLDICGQLREYFNGLRVEFDADIDLIGTPFQLKVWKELQNIGYGKTKSYKDIAQAIGAP
CCHHHHHHHHHHHCCEEEEECCCEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
KAVRAVGGANNQNPLPILIPCHRVIGSNGAMVGYGGGLTKKQLLLGLEGALKISS
HHHHHHCCCCCCCCCEEEEECHHHHCCCCCEEEECCCCCHHHHHHCCCCEEEECC
>Mature Secondary Structure 
SSLKQMYVKEMESPLGLLSIVASERGVCQIHFGEINKAQVTAKLSKAGIRPQWIHLDEN
CHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCC
EDLDICGQLREYFNGLRVEFDADIDLIGTPFQLKVWKELQNIGYGKTKSYKDIAQAIGAP
CCHHHHHHHHHHHCCEEEEECCCEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
KAVRAVGGANNQNPLPILIPCHRVIGSNGAMVGYGGGLTKKQLLLGLEGALKISS
HHHHHHCCCCCCCCCEEEEECHHHHCCCCCEEEECCCCCHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA