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The map label for this gene is prfA

Identifier: 55981909

GI number: 55981909

Start: 1819771

End: 1820835

Strand: Reverse

Name: prfA

Synonym: TTHA1940

Alternate gene names: 55981909

Gene position: 1820835-1819771 (Counterclockwise)

Preceding gene: 55981911

Following gene: 55981908

Centisome position: 98.44

GC content: 67.7

Gene sequence:

>1065_bases
ATGCTGGACAAGCTTGACCGCCTAGAGGAAGAGTACCGGGAGCTGGAGGCGCTCCTCTCCGACCCGGAGGTGCTGAAGGA
CAAGGGGCGCTACCAGAGCCTCTCCCGCCGCTACGCCGAGATGGGGGAGGTGATCGGCCTCATCCGGGAGTACCGGAAGG
TGCTGGAGGACCTGGAGCAGGCGGAAAGCCTTCTTGACGACCCCGAGCTCAAGGAGATGGCCAAGGCGGAGCGGGAGGCC
CTCCTCGCCCGCAAGGAGGCCCTGGAGAAGGAGCTGGAGCGCCACCTCCTGCCTAAGGACCCCATGGACGAAAGGGACGC
CATCGTAGAGATCCGGGCGGGGACGGGAGGGGAGGAGGCCGCCCTCTTCGCCCGCGACCTTTTCAACATGTACCTCCGCT
TCGCCGAGGAGATGGGCTTTGAGACGGAGGTCCTGGACTCCCACCCCACGGACCTCGGGGGCTTCTCCAAGGTGGTCTTT
GAGGTGCGGGGCCCGGGGGCCTACGGCACCTTCAAGTACGAGAGCGGGGTCCACCGGGTGCAACGGGTGCCCGTCACCGA
GACCCAGGGGCGGATCCACACCTCCACCGCCACGGTGGCCGTCCTCCCCAAGGCGGAGGAGGAGGACTTCGCCCTCAACA
TGGACGAGATCCGCATTGACGTGATGCGGGCCTCGGGGCCCGGGGGGCAGGGGGTGAACACCACGGACTCGGCGGTGCGG
GTGGTCCACCTGCCCACGGGGATCATGGTCACCTGCCAGGACTCCCGCAGCCAGATCAAGAACCGGGAGAAGGCCCTCAT
GATCCTAAGAAGCCGTCTCCTGGAGATGAAGCGGGCGGAGGAGGCGGAAAGGCTCCGGAAGACCCGCCTTGCCCAGATCG
GCACCGGGGAGCGCTCGGAGAAGATCCGCACCTACAACTTCCCCCAGTCCCGGGTCACGGACCACCGCATCGGGTTCACC
ACCCACGACCTCGAGGGCGTCCTCTCCGGCCACCTGACCCCCATCCTGGAGGCGCTCAAGCGGGCCGACCAGGAGCGCCA
GCTCGCGGCGCTGGCGGAAGGGTGA

Upstream 100 bases:

>100_bases
CCGCCCAGCAGGGCGTAGAGGAGAAAGGGCTCCATGCCCCCGAGTTTAGGCTAACCTAAAACCGGGGTCAAGGGCCGGGT
GCGCTATCCTGGAAACAGGT

Downstream 100 bases:

>100_bases
TGCGCCGGGAGATCCTGGTGGCGGCGGCCATCCTCCTGGACAGCCGGGGCCGGGTCCTCCTCGTGGGCAACGACTGGGGC
CGCCGGGGCCGGGTGCGCTA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MLDKLDRLEEEYRELEALLSDPEVLKDKGRYQSLSRRYAEMGEVIGLIREYRKVLEDLEQAESLLDDPELKEMAKAEREA
LLARKEALEKELERHLLPKDPMDERDAIVEIRAGTGGEEAALFARDLFNMYLRFAEEMGFETEVLDSHPTDLGGFSKVVF
EVRGPGAYGTFKYESGVHRVQRVPVTETQGRIHTSTATVAVLPKAEEEDFALNMDEIRIDVMRASGPGGQGVNTTDSAVR
VVHLPTGIMVTCQDSRSQIKNREKALMILRSRLLEMKRAEEAERLRKTRLAQIGTGERSEKIRTYNFPQSRVTDHRIGFT
THDLEGVLSGHLTPILEALKRADQERQLAALAEG

Sequences:

>Translated_354_residues
MLDKLDRLEEEYRELEALLSDPEVLKDKGRYQSLSRRYAEMGEVIGLIREYRKVLEDLEQAESLLDDPELKEMAKAEREA
LLARKEALEKELERHLLPKDPMDERDAIVEIRAGTGGEEAALFARDLFNMYLRFAEEMGFETEVLDSHPTDLGGFSKVVF
EVRGPGAYGTFKYESGVHRVQRVPVTETQGRIHTSTATVAVLPKAEEEDFALNMDEIRIDVMRASGPGGQGVNTTDSAVR
VVHLPTGIMVTCQDSRSQIKNREKALMILRSRLLEMKRAEEAERLRKTRLAQIGTGERSEKIRTYNFPQSRVTDHRIGFT
THDLEGVLSGHLTPILEALKRADQERQLAALAEG
>Mature_354_residues
MLDKLDRLEEEYRELEALLSDPEVLKDKGRYQSLSRRYAEMGEVIGLIREYRKVLEDLEQAESLLDDPELKEMAKAEREA
LLARKEALEKELERHLLPKDPMDERDAIVEIRAGTGGEEAALFARDLFNMYLRFAEEMGFETEVLDSHPTDLGGFSKVVF
EVRGPGAYGTFKYESGVHRVQRVPVTETQGRIHTSTATVAVLPKAEEEDFALNMDEIRIDVMRASGPGGQGVNTTDSAVR
VVHLPTGIMVTCQDSRSQIKNREKALMILRSRLLEMKRAEEAERLRKTRLAQIGTGERSEKIRTYNFPQSRVTDHRIGFT
THDLEGVLSGHLTPILEALKRADQERQLAALAEG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=307, Percent_Identity=46.9055374592834, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI34577120, Length=337, Percent_Identity=39.7626112759644, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI166795305, Length=223, Percent_Identity=44.3946188340807, Blast_Score=174, Evalue=8e-44,
Organism=Escherichia coli, GI1787462, Length=356, Percent_Identity=48.5955056179775, Blast_Score=330, Evalue=7e-92,
Organism=Escherichia coli, GI2367172, Length=345, Percent_Identity=36.231884057971, Blast_Score=195, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=282, Percent_Identity=37.5886524822695, Blast_Score=185, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=331, Percent_Identity=42.5981873111783, Blast_Score=251, Evalue=9e-68,
Organism=Drosophila melanogaster, GI19921226, Length=323, Percent_Identity=44.2724458204334, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_THET2 (Q72HB8)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_005546.1
- PDB:   3D5A
- PDB:   3D5C
- PDB:   3MR8
- PDB:   3MS0
- PDBsum:   3D5A
- PDBsum:   3D5C
- PDBsum:   3MR8
- PDBsum:   3MS0
- ProteinModelPortal:   Q72HB8
- SMR:   Q72HB8
- STRING:   Q72HB8
- GeneID:   2774745
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q72HB8
- ProtClustDB:   PRK00591
- BioCyc:   TTHE262724:TT_C1577-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40093; Mature: 40093

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKLDRLEEEYRELEALLSDPEVLKDKGRYQSLSRRYAEMGEVIGLIREYRKVLEDLEQ
CCCHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AESLLDDPELKEMAKAEREALLARKEALEKELERHLLPKDPMDERDAIVEIRAGTGGEEA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHH
ALFARDLFNMYLRFAEEMGFETEVLDSHPTDLGGFSKVVFEVRGPGAYGTFKYESGVHRV
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHH
QRVPVTETQGRIHTSTATVAVLPKAEEEDFALNMDEIRIDVMRASGPGGQGVNTTDSAVR
HCCCCCCCCCEEEECCEEEEEECCCCCCCCCCCHHHHEEEEEECCCCCCCCCCCCCCCEE
VVHLPTGIMVTCQDSRSQIKNREKALMILRSRLLEMKRAEEAERLRKTRLAQIGTGERSE
EEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KIRTYNFPQSRVTDHRIGFTTHDLEGVLSGHLTPILEALKRADQERQLAALAEG
CCEECCCCHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MLDKLDRLEEEYRELEALLSDPEVLKDKGRYQSLSRRYAEMGEVIGLIREYRKVLEDLEQ
CCCHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AESLLDDPELKEMAKAEREALLARKEALEKELERHLLPKDPMDERDAIVEIRAGTGGEEA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHH
ALFARDLFNMYLRFAEEMGFETEVLDSHPTDLGGFSKVVFEVRGPGAYGTFKYESGVHRV
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHH
QRVPVTETQGRIHTSTATVAVLPKAEEEDFALNMDEIRIDVMRASGPGGQGVNTTDSAVR
HCCCCCCCCCEEEECCEEEEEECCCCCCCCCCCHHHHEEEEEECCCCCCCCCCCCCCCEE
VVHLPTGIMVTCQDSRSQIKNREKALMILRSRLLEMKRAEEAERLRKTRLAQIGTGERSE
EEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KIRTYNFPQSRVTDHRIGFTTHDLEGVLSGHLTPILEALKRADQERQLAALAEG
CCEECCCCHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA