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The map label for this gene is purB [H]

Identifier: 55981482

GI number: 55981482

Start: 1438448

End: 1439758

Strand: Direct

Name: purB [H]

Synonym: TTHA1513

Alternate gene names: 55981482

Gene position: 1438448-1439758 (Clockwise)

Preceding gene: 55981480

Following gene: 55981484

Centisome position: 77.76

GC content: 68.19

Gene sequence:

>1311_bases
ATGATCGCCCGCTACCAGACGCCGGAGATGGCCCGCCTCTGGTCGGAGGAAAGCCGCTACCGGATGTGGGCCCGGGTGGA
GGCCTACGCCCTCGAGGCCTGGGAGGCCTTAGGGGAGGTGCCCAAGGGCCTTTCGGCGAGGCTTCTCGCCAAGCTGGAGG
AAAAGCCCCTGGACGGGGCCTTCGCCCGGAGGGTAGCGGAGCTGGAGGCGGTAACCCGCCACGACCTCGTGGCCTTCACC
CGGGCCCTCGCCGAGTGGACGGGGGACGAGGAGGTGGGGCGCTACCTCCACCTGGGCCTTACCAGCTCCGACATCGTGGA
CACGGCGCAAAACGCCCTCCTGGTGGAGGCCCTAGGCCTCGTCCTGGAGGAGCTTAAGGGGGTGGAGGAGGCCTTAAAGG
CCCTCGCCCTCCGCCACAAGCACACCCCCGCCATCGCCCGCACCCACGGGGTCCACGCCGAGCCCACGAGCTTCGGCCTC
CGCTTCCTCTCCTTCCTCGCCGCCTTCCAAAGGGACGAGGAAAGGCTTAAGCGGGCGAGGGAGACCATCGGGGTCGCCAT
GCTCTCGGGCTCCGTGGGCAACTACGCCCACGTCCCCCCCGAGGTGGAGGCCCACGTGGCCTCTAGGCTTGGCCTCAGGC
CCGAGCCCCTTTCCACCCAGGTCGTCCCCCGGGACCGGCACGCCGAGGTCATGGCGGCCCTCGCCATCCTGGGGGGGAAC
ATAGAGCGGGTGGCCGTGGAGCTACGCCACCTCCAGCGCACGGAGGTCCTCGAGGCCCAGGAGCCCTTCCACGAAGGGCA
GACGGGGAGCTCCTCCATGCCCCACAAGAAGAACCCCGTGGGCCTGGAGAACCTCACCGGGGTGGCGAGGCTTTTACGGG
GCTACCTCTTTCCCGCCCTGGAGGACATCGCCCTCTGGCACGAGCGGGACATCTCCCACTCCTCCGTGGAAAGGGTCATC
CTCCCCGACGCCACCACCCTGGCCCACTACGCCCTGAGGCGCCTCAAGGGGATCCTCGAGGGCCTCGAGGTCTTTGAAGA
GAACCTGGCCAGGAACCTGGACCTCACCCGGGGCCTCGTCTACTCCCAGCAGGTCCTCAACGCCCTCATCGCCCGGGGCC
TTTCCCGGAATAAGGCCTACGCCCTCGTCCAGCGGAACGCCTTGAGGAGCTGGAAAGAGGGGAAGAGCTTCCTGGAGCTC
TTGGAGGCCGACCCCGAAAACCCCCTCAAGGGAAAGGAGCTAAGGGCCCTCTTTGACCCCAAGCCCTTCCTCCGGCACGT
GGACGCCATCTACGCCCGGTTCGGGCTCTGA

Upstream 100 bases:

>100_bases
CTCATGGTCCGCCACCCCGAAAGGGGCCGAAGCGCCTCCGTGCCTCCAGTATACCCCCTTCCTTCGCCTCCCCTGCCCCT
TCCCTGGTACCCTTAAGGGC

Downstream 100 bases:

>100_bases
GCACCCCGCCCGCGGCTTTCGCCGCGGCGGGGGCCCCGAAAGGCTTCCCCAAAGCCCTAGTCCGGGCAATCCCCTGTGGC
AAAACCGGGGTGCAGGCGCT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MIARYQTPEMARLWSEESRYRMWARVEAYALEAWEALGEVPKGLSARLLAKLEEKPLDGAFARRVAELEAVTRHDLVAFT
RALAEWTGDEEVGRYLHLGLTSSDIVDTAQNALLVEALGLVLEELKGVEEALKALALRHKHTPAIARTHGVHAEPTSFGL
RFLSFLAAFQRDEERLKRARETIGVAMLSGSVGNYAHVPPEVEAHVASRLGLRPEPLSTQVVPRDRHAEVMAALAILGGN
IERVAVELRHLQRTEVLEAQEPFHEGQTGSSSMPHKKNPVGLENLTGVARLLRGYLFPALEDIALWHERDISHSSVERVI
LPDATTLAHYALRRLKGILEGLEVFEENLARNLDLTRGLVYSQQVLNALIARGLSRNKAYALVQRNALRSWKEGKSFLEL
LEADPENPLKGKELRALFDPKPFLRHVDAIYARFGL

Sequences:

>Translated_436_residues
MIARYQTPEMARLWSEESRYRMWARVEAYALEAWEALGEVPKGLSARLLAKLEEKPLDGAFARRVAELEAVTRHDLVAFT
RALAEWTGDEEVGRYLHLGLTSSDIVDTAQNALLVEALGLVLEELKGVEEALKALALRHKHTPAIARTHGVHAEPTSFGL
RFLSFLAAFQRDEERLKRARETIGVAMLSGSVGNYAHVPPEVEAHVASRLGLRPEPLSTQVVPRDRHAEVMAALAILGGN
IERVAVELRHLQRTEVLEAQEPFHEGQTGSSSMPHKKNPVGLENLTGVARLLRGYLFPALEDIALWHERDISHSSVERVI
LPDATTLAHYALRRLKGILEGLEVFEENLARNLDLTRGLVYSQQVLNALIARGLSRNKAYALVQRNALRSWKEGKSFLEL
LEADPENPLKGKELRALFDPKPFLRHVDAIYARFGL
>Mature_436_residues
MIARYQTPEMARLWSEESRYRMWARVEAYALEAWEALGEVPKGLSARLLAKLEEKPLDGAFARRVAELEAVTRHDLVAFT
RALAEWTGDEEVGRYLHLGLTSSDIVDTAQNALLVEALGLVLEELKGVEEALKALALRHKHTPAIARTHGVHAEPTSFGL
RFLSFLAAFQRDEERLKRARETIGVAMLSGSVGNYAHVPPEVEAHVASRLGLRPEPLSTQVVPRDRHAEVMAALAILGGN
IERVAVELRHLQRTEVLEAQEPFHEGQTGSSSMPHKKNPVGLENLTGVARLLRGYLFPALEDIALWHERDISHSSVERVI
LPDATTLAHYALRRLKGILEGLEVFEENLARNLDLTRGLVYSQQVLNALIARGLSRNKAYALVQRNALRSWKEGKSFLEL
LEADPENPLKGKELRALFDPKPFLRHVDAIYARFGL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=450, Percent_Identity=26, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI183227688, Length=387, Percent_Identity=26.6149870801034, Blast_Score=133, Evalue=4e-31,
Organism=Escherichia coli, GI1787376, Length=314, Percent_Identity=27.7070063694268, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17508577, Length=400, Percent_Identity=25.75, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI32564234, Length=348, Percent_Identity=25.8620689655172, Blast_Score=81, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323391, Length=447, Percent_Identity=25.9507829977629, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24647570, Length=441, Percent_Identity=27.437641723356, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48776; Mature: 48776

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIARYQTPEMARLWSEESRYRMWARVEAYALEAWEALGEVPKGLSARLLAKLEEKPLDGA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
FARRVAELEAVTRHDLVAFTRALAEWTGDEEVGRYLHLGLTSSDIVDTAQNALLVEALGL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEECCCCHHHHHHHHHHHHHHHHHHH
VLEELKGVEEALKALALRHKHTPAIARTHGVHAEPTSFGLRFLSFLAAFQRDEERLKRAR
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ETIGVAMLSGSVGNYAHVPPEVEAHVASRLGLRPEPLSTQVVPRDRHAEVMAALAILGGN
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
IERVAVELRHLQRTEVLEAQEPFHEGQTGSSSMPHKKNPVGLENLTGVARLLRGYLFPAL
HHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EDIALWHERDISHSSVERVILPDATTLAHYALRRLKGILEGLEVFEENLARNLDLTRGLV
HHHHHHHHCCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YSQQVLNALIARGLSRNKAYALVQRNALRSWKEGKSFLELLEADPENPLKGKELRALFDP
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCC
KPFLRHVDAIYARFGL
CHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIARYQTPEMARLWSEESRYRMWARVEAYALEAWEALGEVPKGLSARLLAKLEEKPLDGA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
FARRVAELEAVTRHDLVAFTRALAEWTGDEEVGRYLHLGLTSSDIVDTAQNALLVEALGL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEECCCCHHHHHHHHHHHHHHHHHHH
VLEELKGVEEALKALALRHKHTPAIARTHGVHAEPTSFGLRFLSFLAAFQRDEERLKRAR
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ETIGVAMLSGSVGNYAHVPPEVEAHVASRLGLRPEPLSTQVVPRDRHAEVMAALAILGGN
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
IERVAVELRHLQRTEVLEAQEPFHEGQTGSSSMPHKKNPVGLENLTGVARLLRGYLFPAL
HHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EDIALWHERDISHSSVERVILPDATTLAHYALRRLKGILEGLEVFEENLARNLDLTRGLV
HHHHHHHHCCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YSQQVLNALIARGLSRNKAYALVQRNALRSWKEGKSFLELLEADPENPLKGKELRALFDP
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCC
KPFLRHVDAIYARFGL
CHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]