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The map label for this gene is trmJ [H]

Identifier: 55981151

GI number: 55981151

Start: 1128130

End: 1128864

Strand: Reverse

Name: trmJ [H]

Synonym: TTHA1182

Alternate gene names: 55981151

Gene position: 1128864-1128130 (Counterclockwise)

Preceding gene: 55981152

Following gene: 55981150

Centisome position: 61.03

GC content: 71.7

Gene sequence:

>735_bases
GTGGAGCGGGTGAGGATCGTCCTGGTGGAGCCCCAGGAGCCGATGAACCTGGGGGCGGTGGCCCGGGCCATGCGGAACTT
CGGGCTTTCCCGCCTCTACGTGGTGAACCCCCCGCCCCGGGTGGGCCCGCCCTGGGCCAAGGAGGCCTACTGGCTCGCCG
TCCACGCCGAGGAGGTGCTGGAGAAGGCCCAGGTGGCGGGAAGCCTCCTGGAGGCCCTTCGGGGGGCGCAGCTCGTGGTG
GCCACCACGGGCCGTCCCCGGGAGGTCTACCCCGCCCCCGTCGCCCCGGCCTGGGAGGTGGCGGACCGGGTGGCGGCCTT
CCCGGGGGAGGTGGCCTTGGTCTTCGGGCGGGAGACCTTCGGCCTCACCAACGAGGAGCTGGAGCTCGCCCACCTCATCG
GCTACATCCCCACGGCCCCGGAGCAGCCCTCCTTGAACCTGGCCCAGGCGGTGGTGGTCTTCGCCTACGAGCTCTTTAAG
CGCCAGGGAAAGGGGGTGGGCCTGAGGGAGGGGGGCGAGCCCGCCCCGGTGGAGGCCCTCGAGGCCTTCTTCCAGGACCT
GGGGCGGTACATCCTGGAGATCGGCTTCACCGACGAGCGCCGCTTCCCCTACGCCATGCGCCGCCTCCGGCGGATCTTCC
ACAAGGCCGGCCTCACCCTGGGGGAGGTCCAGATGCTCCGGGGCCTCCTCCACCAGAGCCGCTTCGCCCAGAGGAGAAAG
GATGACGGAGCATAG

Upstream 100 bases:

>100_bases
GGCAAGGTGCCCTCGGGGAGGCCCCCGGAGGAAAGCCCCGCCTTGCCCCTTCCCCTCCCCCTCCTCACGCCCCCGGGGGG
CGGGCTTTAGGAGGTCTTGC

Downstream 100 bases:

>100_bases
CCTCTACCGCCTCGTCCGCCTGGCCCAAAGGCTTCTGCCCCCCGCCATCGCCCTGGTGGTCCTGGCCTTTGAGCTCGCCC
TTTCCCCCTTTGACCACACC

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MERVRIVLVEPQEPMNLGAVARAMRNFGLSRLYVVNPPPRVGPPWAKEAYWLAVHAEEVLEKAQVAGSLLEALRGAQLVV
ATTGRPREVYPAPVAPAWEVADRVAAFPGEVALVFGRETFGLTNEELELAHLIGYIPTAPEQPSLNLAQAVVVFAYELFK
RQGKGVGLREGGEPAPVEALEAFFQDLGRYILEIGFTDERRFPYAMRRLRRIFHKAGLTLGEVQMLRGLLHQSRFAQRRK
DDGA

Sequences:

>Translated_244_residues
MERVRIVLVEPQEPMNLGAVARAMRNFGLSRLYVVNPPPRVGPPWAKEAYWLAVHAEEVLEKAQVAGSLLEALRGAQLVV
ATTGRPREVYPAPVAPAWEVADRVAAFPGEVALVFGRETFGLTNEELELAHLIGYIPTAPEQPSLNLAQAVVVFAYELFK
RQGKGVGLREGGEPAPVEALEAFFQDLGRYILEIGFTDERRFPYAMRRLRRIFHKAGLTLGEVQMLRGLLHQSRFAQRRK
DDGA
>Mature_244_residues
MERVRIVLVEPQEPMNLGAVARAMRNFGLSRLYVVNPPPRVGPPWAKEAYWLAVHAEEVLEKAQVAGSLLEALRGAQLVV
ATTGRPREVYPAPVAPAWEVADRVAAFPGEVALVFGRETFGLTNEELELAHLIGYIPTAPEQPSLNLAQAVVVFAYELFK
RQGKGVGLREGGEPAPVEALEAFFQDLGRYILEIGFTDERRFPYAMRRLRRIFHKAGLTLGEVQMLRGLLHQSRFAQRRK
DDGA

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=251, Percent_Identity=39.4422310756972, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1790865, Length=161, Percent_Identity=37.888198757764, Blast_Score=104, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27166; Mature: 27166

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERVRIVLVEPQEPMNLGAVARAMRNFGLSRLYVVNPPPRVGPPWAKEAYWLAVHAEEVL
CCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEEHHHHH
EKAQVAGSLLEALRGAQLVVATTGRPREVYPAPVAPAWEVADRVAAFPGEVALVFGRETF
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCHHC
GLTNEELELAHLIGYIPTAPEQPSLNLAQAVVVFAYELFKRQGKGVGLREGGEPAPVEAL
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
EAFFQDLGRYILEIGFTDERRFPYAMRRLRRIFHKAGLTLGEVQMLRGLLHQSRFAQRRK
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
DDGA
CCCC
>Mature Secondary Structure
MERVRIVLVEPQEPMNLGAVARAMRNFGLSRLYVVNPPPRVGPPWAKEAYWLAVHAEEVL
CCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEEHHHHH
EKAQVAGSLLEALRGAQLVVATTGRPREVYPAPVAPAWEVADRVAAFPGEVALVFGRETF
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCHHC
GLTNEELELAHLIGYIPTAPEQPSLNLAQAVVVFAYELFKRQGKGVGLREGGEPAPVEAL
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
EAFFQDLGRYILEIGFTDERRFPYAMRRLRRIFHKAGLTLGEVQMLRGLLHQSRFAQRRK
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
DDGA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA