Click here to switch to the map view.

The map label for this gene is pnp [H]

Identifier: 55981108

GI number: 55981108

Start: 1079898

End: 1082039

Strand: Direct

Name: pnp [H]

Synonym: TTHA1139

Alternate gene names: 55981108

Gene position: 1079898-1082039 (Clockwise)

Preceding gene: 55981107

Following gene: 55981109

Centisome position: 58.38

GC content: 69.23

Gene sequence:

>2142_bases
ATGGAAGGCACACCCAATGTCCCCCAAGCCCACCGCTACGAGCTCACCCTGGCGGGGCGGCCCCTCGTCCTGGAGACGGG
CAAGTACGCCAAGCAGGCCTCGGGGTCCGTCCTGGTCCGCTACGCCGACACCGTGGTCCTGGCCACGGCCCAAGCCTCGG
AGACGCCGGTGGAGGCCGACTTCCTCCCCCTCACGGTGGAGTTTGAGGAAAGGCACTACGCCGTGGGGAAGATCCCGGGA
AGCTTCATGCGCCGGGAGGGGCGTCCCGGGGAGAAGGCCATCCTCTCCGCCCGCATGACGGACCGCCCCATCAGGCCCCT
CTTCCCCAAGGGGTTCCGCCACGAGGTCCAGATCATCGTCACCGTCCTCTCCGCCGACCAGAAGAACCCGCCGGACATCC
TGGGCCCCATCGCAGCGAGCGCCGCCCTCATGCTCTCGGACATCCCCTGGGAGGGCCCCATCGCCGCCGTGCGGGTGGGC
CTCATCGGCGGGAGCTTCGTCCTGAACCCCACCCTGCAGGAGCTGGAGGAAAGCCAGCTGGACCTGGTGGTGGCGGGGAG
CAAAGAGGCCATCCTCATGGTGGAGGCGGAGGCCGGGGAGGTGGACGAGGAGACCCTGGTCCAGGCCCTGGAGTTCGCCC
ACAAGGAGATGCAGCCCATCCTGGAGCTCCAGGAGGCCATGGCCCGGGAACTCGCCAAACCCAAGATGGCCTGGACCCCC
CCGGAAAGCCTCCCCGAGGAGGAGAAGGAGGCCCTCTACCGCCTGGCCCTGGAGCGGGGGCTTTCCCAGGTCCTGCAGAC
GGCGAGCAAGGGGGAAAGGAGCCGGGCCCTTGCGGAGTTCGCCGAACGCCTCATCGCCGAGGCCCTCCCCAAGGGGGAGG
ACGGCACCCCGGACGAGGGCAAGAAGCCCCTCTACGAGAGCGCCTTTGACGAGGTGGTGCGCAGGGAGCTCAGGAGGCTC
GTCCTCGAGGAGGGCAAGCGGGCGGACGGCCGCGGGCCCAAGGACCTCAGGCCCATCTGGATTGAGGTGGACGTCCTGCC
CCGGGCCCACGGCTCCGCCGTCTTCACCCGGGGCGAAACCCAGGTCCTGGGCACCGTCACCCTGGGCACGGGCCGGGACG
AGCAGATCCTGGACGACCTGGGCATAGACGAGACCGAGAAGTTCCTCGTCCACTACAACTTCCCCCCCTTCTCCACCGGG
GAGGTGCGGCGCCTACGGGGGGTTTCCCGCCGCGAGGTGGGGCACGGAAATCTGGCCAAGCGGGCCCTCAAGGCGGTGAT
GCCCAAGGAGGAGGACTTCCCTTACACCATCCGCGTGGTGGGGGACGTCCTGGAGTCCAACGGCAGTAGCTCCATGGCCA
CGGTCTGCGCCGGCTGCCTCGCCCTCATGGACGCGGGCGTGCCCATCCGGGCCCCGGTGGCCGGGGTGGCCATGGGCTTG
GTGTGGGAGGAGAACCGGGCGGTGATCCTCACGGACATCCTGGGCCTCGAGGACGCCCTCGGCGACATGGACTTCAAGGT
GGCCGGGACCCGCAAGGGGGTCACCGCCTTGCAGATGGACAACAAGGTGGGGGGCCTTCCCCGCGAGGTCCTGAAGGAGG
CCCTCCTCCAGGCCCGGGAGGCCAGGCTCAAGATCCTGGACCTGATGGAGGCCGTCCTCCCCGCCCCCCGGCCCGAGCTC
AAGCCCTTCGCCCCCCGGATCCTCTCCCTCAAGGTGCCCGTGGAGAAGATCGGGCTCGTCATCGGCCCCGGGGGCAAGAA
CGTGCGCGCCCTGGAGGAGCTTGGGGTGGAGGTGGACATTGAGGAGGACGGGACGGTCCGCATCTACTCCAGCGACCTCC
AGGCCGCCCTCGAGGCGAAGAAGCGCATAGAGGACCTCACCCGCGAGGCCAAGGTGGGCGAGATCTACGAGGGCACCGTC
ACCCGCATCACCCCCTTCGGCGCCTTCATCAGCCTCTTCCCCGGCACCGAGGGGCTTCTCCACATCAGCCAGATCGCCCC
GGGCCGCGTGGCGCGGGTGGAGGACCACCTCAAGGTGGGGGACGTGATCAAGGTCAAGGTCCACCGCATTGACGAGCGGG
GCAAGATTGACCTGATCCGCCCCGAGCTGGAAGGCAAGATCCCCCCCAGGCGGCGCAGGTAG

Upstream 100 bases:

>100_bases
TACCGGGCCCTTATTGAGAAGCTGGGCATCCGGGGTTAAACTGGGGAACAGGTCGGGGGCGGGGCCGAAAGCTCCGCCCT
TCGCTTTTGGAAGGAGCGCC

Downstream 100 bases:

>100_bases
CCCCTGGGGGCTTGACCTCACGCGCGTCCCGGCCAGGCCGGGAAGATGGGAGGCGTATGGAAAACCAAGAACGGAAGCCG
AGGAGGAGGCGCCGGCGGAG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MEGTPNVPQAHRYELTLAGRPLVLETGKYAKQASGSVLVRYADTVVLATAQASETPVEADFLPLTVEFEERHYAVGKIPG
SFMRREGRPGEKAILSARMTDRPIRPLFPKGFRHEVQIIVTVLSADQKNPPDILGPIAASAALMLSDIPWEGPIAAVRVG
LIGGSFVLNPTLQELEESQLDLVVAGSKEAILMVEAEAGEVDEETLVQALEFAHKEMQPILELQEAMARELAKPKMAWTP
PESLPEEEKEALYRLALERGLSQVLQTASKGERSRALAEFAERLIAEALPKGEDGTPDEGKKPLYESAFDEVVRRELRRL
VLEEGKRADGRGPKDLRPIWIEVDVLPRAHGSAVFTRGETQVLGTVTLGTGRDEQILDDLGIDETEKFLVHYNFPPFSTG
EVRRLRGVSRREVGHGNLAKRALKAVMPKEEDFPYTIRVVGDVLESNGSSSMATVCAGCLALMDAGVPIRAPVAGVAMGL
VWEENRAVILTDILGLEDALGDMDFKVAGTRKGVTALQMDNKVGGLPREVLKEALLQAREARLKILDLMEAVLPAPRPEL
KPFAPRILSLKVPVEKIGLVIGPGGKNVRALEELGVEVDIEEDGTVRIYSSDLQAALEAKKRIEDLTREAKVGEIYEGTV
TRITPFGAFISLFPGTEGLLHISQIAPGRVARVEDHLKVGDVIKVKVHRIDERGKIDLIRPELEGKIPPRRRR

Sequences:

>Translated_713_residues
MEGTPNVPQAHRYELTLAGRPLVLETGKYAKQASGSVLVRYADTVVLATAQASETPVEADFLPLTVEFEERHYAVGKIPG
SFMRREGRPGEKAILSARMTDRPIRPLFPKGFRHEVQIIVTVLSADQKNPPDILGPIAASAALMLSDIPWEGPIAAVRVG
LIGGSFVLNPTLQELEESQLDLVVAGSKEAILMVEAEAGEVDEETLVQALEFAHKEMQPILELQEAMARELAKPKMAWTP
PESLPEEEKEALYRLALERGLSQVLQTASKGERSRALAEFAERLIAEALPKGEDGTPDEGKKPLYESAFDEVVRRELRRL
VLEEGKRADGRGPKDLRPIWIEVDVLPRAHGSAVFTRGETQVLGTVTLGTGRDEQILDDLGIDETEKFLVHYNFPPFSTG
EVRRLRGVSRREVGHGNLAKRALKAVMPKEEDFPYTIRVVGDVLESNGSSSMATVCAGCLALMDAGVPIRAPVAGVAMGL
VWEENRAVILTDILGLEDALGDMDFKVAGTRKGVTALQMDNKVGGLPREVLKEALLQAREARLKILDLMEAVLPAPRPEL
KPFAPRILSLKVPVEKIGLVIGPGGKNVRALEELGVEVDIEEDGTVRIYSSDLQAALEAKKRIEDLTREAKVGEIYEGTV
TRITPFGAFISLFPGTEGLLHISQIAPGRVARVEDHLKVGDVIKVKVHRIDERGKIDLIRPELEGKIPPRRRR
>Mature_713_residues
MEGTPNVPQAHRYELTLAGRPLVLETGKYAKQASGSVLVRYADTVVLATAQASETPVEADFLPLTVEFEERHYAVGKIPG
SFMRREGRPGEKAILSARMTDRPIRPLFPKGFRHEVQIIVTVLSADQKNPPDILGPIAASAALMLSDIPWEGPIAAVRVG
LIGGSFVLNPTLQELEESQLDLVVAGSKEAILMVEAEAGEVDEETLVQALEFAHKEMQPILELQEAMARELAKPKMAWTP
PESLPEEEKEALYRLALERGLSQVLQTASKGERSRALAEFAERLIAEALPKGEDGTPDEGKKPLYESAFDEVVRRELRRL
VLEEGKRADGRGPKDLRPIWIEVDVLPRAHGSAVFTRGETQVLGTVTLGTGRDEQILDDLGIDETEKFLVHYNFPPFSTG
EVRRLRGVSRREVGHGNLAKRALKAVMPKEEDFPYTIRVVGDVLESNGSSSMATVCAGCLALMDAGVPIRAPVAGVAMGL
VWEENRAVILTDILGLEDALGDMDFKVAGTRKGVTALQMDNKVGGLPREVLKEALLQAREARLKILDLMEAVLPAPRPEL
KPFAPRILSLKVPVEKIGLVIGPGGKNVRALEELGVEVDIEEDGTVRIYSSDLQAALEAKKRIEDLTREAKVGEIYEGTV
TRITPFGAFISLFPGTEGLLHISQIAPGRVARVEDHLKVGDVIKVKVHRIDERGKIDLIRPELEGKIPPRRRR

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=717, Percent_Identity=39.7489539748954, Blast_Score=463, Evalue=1e-130,
Organism=Escherichia coli, GI145693187, Length=680, Percent_Identity=50.4411764705882, Blast_Score=624, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI115534063, Length=742, Percent_Identity=36.6576819407008, Blast_Score=384, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281362905, Length=717, Percent_Identity=40.1673640167364, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651641, Length=717, Percent_Identity=40.1673640167364, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651643, Length=717, Percent_Identity=40.1673640167364, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI161079377, Length=662, Percent_Identity=40.785498489426, Blast_Score=417, Evalue=1e-116,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 78148; Mature: 78148

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGTPNVPQAHRYELTLAGRPLVLETGKYAKQASGSVLVRYADTVVLATAQASETPVEAD
CCCCCCCCCCCEEEEEECCCCEEEECCCHHHHCCCCEEEEEECEEEEEECCCCCCCCCCC
FLPLTVEFEERHYAVGKIPGSFMRREGRPGEKAILSARMTDRPIRPLFPKGFRHEVQIIV
EEEEEEEECCCEEEEECCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
TVLSADQKNPPDILGPIAASAALMLSDIPWEGPIAAVRVGLIGGSFVLNPTLQELEESQL
EEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCCEEECCHHHHHHHCCC
DLVVAGSKEAILMVEAEAGEVDEETLVQALEFAHKEMQPILELQEAMARELAKPKMAWTP
CEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PESLPEEEKEALYRLALERGLSQVLQTASKGERSRALAEFAERLIAEALPKGEDGTPDEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KKPLYESAFDEVVRRELRRLVLEEGKRADGRGPKDLRPIWIEVDVLPRAHGSAVFTRGET
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCE
QVLGTVTLGTGRDEQILDDLGIDETEKFLVHYNFPPFSTGEVRRLRGVSRREVGHGNLAK
EEEEEEEECCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHCCHHHCCCCCHHHH
RALKAVMPKEEDFPYTIRVVGDVLESNGSSSMATVCAGCLALMDAGVPIRAPVAGVAMGL
HHHHHHCCCCCCCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHEEE
VWEENRAVILTDILGLEDALGDMDFKVAGTRKGVTALQMDNKVGGLPREVLKEALLQARE
EEECCCEEEEEEHHHHHHHHCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
ARLKILDLMEAVLPAPRPELKPFAPRILSLKVPVEKIGLVIGPGGKNVRALEELGVEVDI
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHCCEEECCCCCCHHHHHHCCCEEEE
EEDGTVRIYSSDLQAALEAKKRIEDLTREAKVGEIYEGTVTRITPFGAFISLFPGTEGLL
CCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCE
HISQIAPGRVARVEDHLKVGDVIKVKVHRIDERGKIDLIRPELEGKIPPRRRR
EEECCCCCCHHHHHHHCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MEGTPNVPQAHRYELTLAGRPLVLETGKYAKQASGSVLVRYADTVVLATAQASETPVEAD
CCCCCCCCCCCEEEEEECCCCEEEECCCHHHHCCCCEEEEEECEEEEEECCCCCCCCCCC
FLPLTVEFEERHYAVGKIPGSFMRREGRPGEKAILSARMTDRPIRPLFPKGFRHEVQIIV
EEEEEEEECCCEEEEECCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
TVLSADQKNPPDILGPIAASAALMLSDIPWEGPIAAVRVGLIGGSFVLNPTLQELEESQL
EEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCCEEECCHHHHHHHCCC
DLVVAGSKEAILMVEAEAGEVDEETLVQALEFAHKEMQPILELQEAMARELAKPKMAWTP
CEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PESLPEEEKEALYRLALERGLSQVLQTASKGERSRALAEFAERLIAEALPKGEDGTPDEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KKPLYESAFDEVVRRELRRLVLEEGKRADGRGPKDLRPIWIEVDVLPRAHGSAVFTRGET
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCE
QVLGTVTLGTGRDEQILDDLGIDETEKFLVHYNFPPFSTGEVRRLRGVSRREVGHGNLAK
EEEEEEEECCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHCCHHHCCCCCHHHH
RALKAVMPKEEDFPYTIRVVGDVLESNGSSSMATVCAGCLALMDAGVPIRAPVAGVAMGL
HHHHHHCCCCCCCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHEEE
VWEENRAVILTDILGLEDALGDMDFKVAGTRKGVTALQMDNKVGGLPREVLKEALLQARE
EEECCCEEEEEEHHHHHHHHCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
ARLKILDLMEAVLPAPRPELKPFAPRILSLKVPVEKIGLVIGPGGKNVRALEELGVEVDI
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHCCEEECCCCCCHHHHHHCCCEEEE
EEDGTVRIYSSDLQAALEAKKRIEDLTREAKVGEIYEGTVTRITPFGAFISLFPGTEGLL
CCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCE
HISQIAPGRVARVEDHLKVGDVIKVKVHRIDERGKIDLIRPELEGKIPPRRRR
EEECCCCCCHHHHHHHCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA