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The map label for this gene is ligA [H]

Identifier: 55981066

GI number: 55981066

Start: 1042879

End: 1044909

Strand: Reverse

Name: ligA [H]

Synonym: TTHA1097

Alternate gene names: 55981066

Gene position: 1044909-1042879 (Counterclockwise)

Preceding gene: 55981069

Following gene: 55981064

Centisome position: 56.49

GC content: 66.37

Gene sequence:

>2031_bases
ATGACCCTGGAAGAGGCGAGGAAGCGGGTAAACGAGTTACGGGACCTCATCCGCTACCACAACTACCGCTACTACGTCCT
GGCGGACCCGGAGATCTCCGACGCCGAGTACGACCGGCTTCTTAGGGAGCTCAAGGAGCTTGAGGAGCGCTTCCCCGAGC
TCAAAAGCCCGGACTCCCCCACCCTTCAGGTGGGGGCGAGGCCTTTGGAGGCCACCTTCCGCCCCGTCCGCCACCCCACC
CGCATGTACTCCTTGGACAACGCCTTTAACCTTGACGAGCTCAAGGCCTTTGAGGAGCGGATAGAACGGGCCCTGGGGCG
GAAGGGCCCCTTCGCCTACACCGTGGAGCACAAGGTGGACGGGCTTTCCGTGAACCTCTACTACGAGGAGGGGGTCCTGG
TCTACGGGGCCACCCGGGGGGACGGGGAGGTGGGGGAGGAGGTGACCCAGAACCTCCTCACCATCCCCACCATCCCGAGG
AGGCTCAAGGGGGTGCCGGAGCGCCTCGAGGTCCGGGGGGAGGTCTACATGCCCATAGAGGCCTTCCTCCGGCTCAACGA
GGAGCTGGAGGAGCGGGGGGAGAGGATCTTCAAAAACCCTAGGAATGCGGCGGCGGGTTCCTTAAGGCAAAAAGACCCCC
GCATCACCGCCAAGCGGGGCCTCAGGGCCACCTTCTACGCCTTAGGGCTTGGGCTGGAGGAGGTGGAGAGGGAAGGGGTG
GCGACCCAGTTTGCCCTCCTCCACTGGCTCAAGGAAAAAGGCTTCCCCGTGGAGCACGGCTACGCCCGGGCCGTGGGGGC
GGAAGGGGTGGAGGCGGTCTACCAGGACTGGCTCAAGAAGCGGCGGGCGCTTCCCTTTGAGGCGGACGGGGTGGTGGTGA
AGCTGGACGAGCTTGCCCTTTGGCGGGAGCTCGGCTACACCGCCCGCGCCCCCCGGTTCGCCATCGCCTACAAGTTCCCC
GCCGAGGAGAAGGAGACCCGGCTTTTGGACGTGGTCTTCCAGGTGGGGCGCACCGGGCGGGTGACCCCCGTGGGGATCCT
CGAGCCCGTCTTCCTAGAGGGCAGCGAGGTCTCCCGGGTCACCCTGCACAACGAGAGCTACATAGAGGAGTTGGACATCC
GCATCGGGGACTGGGTTTTGGTGCACAAGGCGGGCGGGGTCATCCCCGAGGTCCTCCGGGTCCTCAAGGAGAGGCGCACG
GGGGAGGAAAGGCCCATTCGCTGGCCCGAGACCTGCCCCGAGTGCGGCCACCGCCTCCTCAAGGAGGGGAAGGTCCACCG
CTGCCCCAACCCCTTGTGCCCCGCCAAGCGCTTTGAGGCCATCCGCCACTTCGCCTCCCGCAAGGCCATGGACATCCAGG
GCCTGGGGGAAAAGCTCATTGAGAGGCTTTTGGAAAAGGGGCTGGTCAAGGACGTGGCCGACCTCTACCGCTTGAGAAAG
GAAGACCTGGTGGGCCTGGAGCGCATGGGGGAGAAGAGCGCCCAAAACCTCCTCCGCCAGATAGAGGAGAGCAAGAAAAG
AGGCCTGGAGCGCCTCCTCTACGCCTTGGGGCTTCCCGGGGTGGGGGAGGTCTTGGCCCGGAACCTGGCGGCCCGCTTCG
GGAACATGGACCGCCTCCTCGAGGCCAGCCTGGAGGAGCTCCTGGAGGTGGAGGAGGTGGGGGAGCTCACGGCGAGGGCC
ATCCTGGAGACCTTGAAGGACCCCGCCTTCCGCGACCTGGTACGGAGGCTCAAGGAGGCGGGGGTGGAGATGGAGGCCAA
GGAGAAGGGCGGGGAGGCCCTTAAAGGGCTCACCTTCGTGATCACCGGGGAGCTTTCCCGCCCCCGGGAAGAGGTGAAGG
CCCTCCTAAGGCGCCTCGGGGCCAAGGTGACGGACTCCGTGAGCCGGAAGACGAGCTACCTCGTGGTGGGGGAGAACCCG
GGGAGCAAGCTGGAGAAGGCCAGGGCCCTCGGGGTCCCCACCCTCACGGAGGAGGAGCTCTACCGGCTCCTGGAGGCGCG
GACGGGGAAGAAGGCGGAGGAGCTCGTCTAA

Upstream 100 bases:

>100_bases
AGGGTGAGCAACCACAAAAGCCTCCTCATAACGCCTCCTTTCGGAATAGGGGATGCGCCCCTAGTCCAAGGGAAAGTATA
GCCCAAGGTACACTAGGGCC

Downstream 100 bases:

>100_bases
AGGGCTTCCCCTGGCCCGAAGACCTTCTCTAGGGCCTCCTTCAGGCGGCTTCCCCCAAGGCCCAAGGAGGAAAGGGCCTT
CTCGTAGACCTCAGGGGTCA

Product: DNA ligase [NAD+]

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 676; Mature: 675

Protein sequence:

>676_residues
MTLEEARKRVNELRDLIRYHNYRYYVLADPEISDAEYDRLLRELKELEERFPELKSPDSPTLQVGARPLEATFRPVRHPT
RMYSLDNAFNLDELKAFEERIERALGRKGPFAYTVEHKVDGLSVNLYYEEGVLVYGATRGDGEVGEEVTQNLLTIPTIPR
RLKGVPERLEVRGEVYMPIEAFLRLNEELEERGERIFKNPRNAAAGSLRQKDPRITAKRGLRATFYALGLGLEEVEREGV
ATQFALLHWLKEKGFPVEHGYARAVGAEGVEAVYQDWLKKRRALPFEADGVVVKLDELALWRELGYTARAPRFAIAYKFP
AEEKETRLLDVVFQVGRTGRVTPVGILEPVFLEGSEVSRVTLHNESYIEELDIRIGDWVLVHKAGGVIPEVLRVLKERRT
GEERPIRWPETCPECGHRLLKEGKVHRCPNPLCPAKRFEAIRHFASRKAMDIQGLGEKLIERLLEKGLVKDVADLYRLRK
EDLVGLERMGEKSAQNLLRQIEESKKRGLERLLYALGLPGVGEVLARNLAARFGNMDRLLEASLEELLEVEEVGELTARA
ILETLKDPAFRDLVRRLKEAGVEMEAKEKGGEALKGLTFVITGELSRPREEVKALLRRLGAKVTDSVSRKTSYLVVGENP
GSKLEKARALGVPTLTEEELYRLLEARTGKKAEELV

Sequences:

>Translated_676_residues
MTLEEARKRVNELRDLIRYHNYRYYVLADPEISDAEYDRLLRELKELEERFPELKSPDSPTLQVGARPLEATFRPVRHPT
RMYSLDNAFNLDELKAFEERIERALGRKGPFAYTVEHKVDGLSVNLYYEEGVLVYGATRGDGEVGEEVTQNLLTIPTIPR
RLKGVPERLEVRGEVYMPIEAFLRLNEELEERGERIFKNPRNAAAGSLRQKDPRITAKRGLRATFYALGLGLEEVEREGV
ATQFALLHWLKEKGFPVEHGYARAVGAEGVEAVYQDWLKKRRALPFEADGVVVKLDELALWRELGYTARAPRFAIAYKFP
AEEKETRLLDVVFQVGRTGRVTPVGILEPVFLEGSEVSRVTLHNESYIEELDIRIGDWVLVHKAGGVIPEVLRVLKERRT
GEERPIRWPETCPECGHRLLKEGKVHRCPNPLCPAKRFEAIRHFASRKAMDIQGLGEKLIERLLEKGLVKDVADLYRLRK
EDLVGLERMGEKSAQNLLRQIEESKKRGLERLLYALGLPGVGEVLARNLAARFGNMDRLLEASLEELLEVEEVGELTARA
ILETLKDPAFRDLVRRLKEAGVEMEAKEKGGEALKGLTFVITGELSRPREEVKALLRRLGAKVTDSVSRKTSYLVVGENP
GSKLEKARALGVPTLTEEELYRLLEARTGKKAEELV
>Mature_675_residues
TLEEARKRVNELRDLIRYHNYRYYVLADPEISDAEYDRLLRELKELEERFPELKSPDSPTLQVGARPLEATFRPVRHPTR
MYSLDNAFNLDELKAFEERIERALGRKGPFAYTVEHKVDGLSVNLYYEEGVLVYGATRGDGEVGEEVTQNLLTIPTIPRR
LKGVPERLEVRGEVYMPIEAFLRLNEELEERGERIFKNPRNAAAGSLRQKDPRITAKRGLRATFYALGLGLEEVEREGVA
TQFALLHWLKEKGFPVEHGYARAVGAEGVEAVYQDWLKKRRALPFEADGVVVKLDELALWRELGYTARAPRFAIAYKFPA
EEKETRLLDVVFQVGRTGRVTPVGILEPVFLEGSEVSRVTLHNESYIEELDIRIGDWVLVHKAGGVIPEVLRVLKERRTG
EERPIRWPETCPECGHRLLKEGKVHRCPNPLCPAKRFEAIRHFASRKAMDIQGLGEKLIERLLEKGLVKDVADLYRLRKE
DLVGLERMGEKSAQNLLRQIEESKKRGLERLLYALGLPGVGEVLARNLAARFGNMDRLLEASLEELLEVEEVGELTARAI
LETLKDPAFRDLVRRLKEAGVEMEAKEKGGEALKGLTFVITGELSRPREEVKALLRRLGAKVTDSVSRKTSYLVVGENPG
SKLEKARALGVPTLTEEELYRLLEARTGKKAEELV

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=88, Percent_Identity=50, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1788750, Length=673, Percent_Identity=46.5081723625557, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI87082305, Length=520, Percent_Identity=25, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 76907; Mature: 76775

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLEEARKRVNELRDLIRYHNYRYYVLADPEISDAEYDRLLRELKELEERFPELKSPDSP
CCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
TLQVGARPLEATFRPVRHPTRMYSLDNAFNLDELKAFEERIERALGRKGPFAYTVEHKVD
CEEECCCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
GLSVNLYYEEGVLVYGATRGDGEVGEEVTQNLLTIPTIPRRLKGVPERLEVRGEVYMPIE
CEEEEEEEECCEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHHCCCCEECCHH
AFLRLNEELEERGERIFKNPRNAAAGSLRQKDPRITAKRGLRATFYALGLGLEEVEREGV
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHCCHHHHHHHHCCHHHHHHCCH
ATQFALLHWLKEKGFPVEHGYARAVGAEGVEAVYQDWLKKRRALPFEADGVVVKLDELAL
HHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHH
WRELGYTARAPRFAIAYKFPAEEKETRLLDVVFQVGRTGRVTPVGILEPVFLEGSEVSRV
HHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHCCCHHHCCCCCCEEE
TLHNESYIEELDIRIGDWVLVHKAGGVIPEVLRVLKERRTGEERPIRWPETCPECGHRLL
EECCHHHHHHHCCEECCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
KEGKVHRCPNPLCPAKRFEAIRHFASRKAMDIQGLGEKLIERLLEKGLVKDVADLYRLRK
HCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDLVGLERMGEKSAQNLLRQIEESKKRGLERLLYALGLPGVGEVLARNLAARFGNMDRLL
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHH
EASLEELLEVEEVGELTARAILETLKDPAFRDLVRRLKEAGVEMEAKEKGGEALKGLTFV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEHHHCCCHHHCCEEEE
ITGELSRPREEVKALLRRLGAKVTDSVSRKTSYLVVGENPGSKLEKARALGVPTLTEEEL
EECCCCCCHHHHHHHHHHHCCHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCHHHH
YRLLEARTGKKAEELV
HHHHHHHCCCHHHHCC
>Mature Secondary Structure 
TLEEARKRVNELRDLIRYHNYRYYVLADPEISDAEYDRLLRELKELEERFPELKSPDSP
CHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
TLQVGARPLEATFRPVRHPTRMYSLDNAFNLDELKAFEERIERALGRKGPFAYTVEHKVD
CEEECCCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
GLSVNLYYEEGVLVYGATRGDGEVGEEVTQNLLTIPTIPRRLKGVPERLEVRGEVYMPIE
CEEEEEEEECCEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHHCCCCEECCHH
AFLRLNEELEERGERIFKNPRNAAAGSLRQKDPRITAKRGLRATFYALGLGLEEVEREGV
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHCCHHHHHHHHCCHHHHHHCCH
ATQFALLHWLKEKGFPVEHGYARAVGAEGVEAVYQDWLKKRRALPFEADGVVVKLDELAL
HHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHH
WRELGYTARAPRFAIAYKFPAEEKETRLLDVVFQVGRTGRVTPVGILEPVFLEGSEVSRV
HHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHCCCHHHCCCCCCEEE
TLHNESYIEELDIRIGDWVLVHKAGGVIPEVLRVLKERRTGEERPIRWPETCPECGHRLL
EECCHHHHHHHCCEECCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
KEGKVHRCPNPLCPAKRFEAIRHFASRKAMDIQGLGEKLIERLLEKGLVKDVADLYRLRK
HCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDLVGLERMGEKSAQNLLRQIEESKKRGLERLLYALGLPGVGEVLARNLAARFGNMDRLL
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHH
EASLEELLEVEEVGELTARAILETLKDPAFRDLVRRLKEAGVEMEAKEKGGEALKGLTFV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEHHHCCCHHHCCEEEE
ITGELSRPREEVKALLRRLGAKVTDSVSRKTSYLVVGENPGSKLEKARALGVPTLTEEEL
EECCCCCCHHHHHHHHHHHCCHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCHHHH
YRLLEARTGKKAEELV
HHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA